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The map label for this gene is ppsA [H]

Identifier: 113969933

GI number: 113969933

Start: 1881795

End: 1884164

Strand: Direct

Name: ppsA [H]

Synonym: Shewmr4_1592

Alternate gene names: 113969933

Gene position: 1881795-1884164 (Clockwise)

Preceding gene: 113969928

Following gene: 113969934

Centisome position: 39.98

GC content: 48.27

Gene sequence:

>2370_bases
GTGCAGCAATACGTACTCTGGTATCAGGAATTAGGCATGGGTGACGTCAACTTGGTTGGCGGTAAAAATGCTTCTCTTGG
CGAGATGATCAGCAATCTAGCTAATGCCGGTGTTCAAGTACCTGGCGGATTTGCGACTACTTCTTATGCGTTCAATGAGT
TCCTTGAACAAAGTGGAGTAAACCAGAAGATTTATGACATCCTAGCAACATTGGATGTAGATGATGTCAATGCACTGGCA
CAAGTAGGTGCACAGATCAGACAATGGGTTATCGACACCCCGTTCCAACCAGCACTCGAGCAAGCGATTCGTGAAGCTTA
CGACAAACTTGCGGCTGAAACGAGCGATGCATCATTCGCGGTTCGTTCTTCTGCCACCGCAGAAGATATGCCAGATGCTT
CTTTTGCTGGTCAGCAAGAAACCTTCTTAAACGTGAAAGGATTTGACTCAGTACTCGTAGCCATTAAGCACGTGTTTGCG
TCACTCTTTAACGACCGTGCAATTTCATACCGTGTTCACCAAGGTTATGAGCACCACGGTGTTGCCCTGTCTGCCGGCGT
ACAACGCATGGTTCGTTCAGACAAAGCGGCATCAGGTGTGATGTTCACCATGGACACTGAATCAGGCAACAACGATGTGG
TGTTTATCACTTCATCTTTCGGTCTGGGTGAAATGGTTGTTCAAGGTGCGGTAAACCCTGACGAATTCTATGTTCACAAA
CCCATCCTCACTCAAGGCCATAAAGCCGTTGTTCGCCGTAATATCGGTAGCAAGCTCATCCAGATGGTGTATTCCGATGA
TGCTTCTCACGGCAAACAAGTGAAAATTGAAGACGTTGCTGCTGAAAAACGTCGTCAATTCTCTATCAACGACGCCGAAG
TGATGGAATTGGCTAAACAAGCTATGGTTATCGAAAAACACTACGGTCGTCCAATGGACATCGAGTGGGCAAAAGACGGT
AACGATGGCAAACTTTATATCGTTCAAGCGCGTCCAGAAACGGTTCGTAGCCGTGAAGATGTACAATTAATTGAACGTTA
CCACTTAAAGAGCCGTGGCGCGGTGATCTCTGAAGGTCGTGCTATCGGTCATAAAGTCGGTTCAGGTGTGGCTAAGGTAT
TAACCTCTATCGCGGATATGGATCAAATCCAACCCGGCGATGTGTTAGTAACCGACATGACTGACCCAGATTGGGAACCT
ATCATGAAGCGCGCCAGCGCCATCGTGACTAACCGTGGCGGCCGTACCTGTCACGCGGCGATTATTGCCCGTGAATTAGG
TGTACCAGCAGTGGTAGGTTGTGGTGACGTGACCGACCGCATCAAGAATGGTCAGTTAGTGACTGTTTCTTGCGCCGAAG
GCGACACAGGTTACATCTACGAAGGTAAGCAAGAGTTCGAAGTGGTTTCAAACCGCGTTGACGCACTGCCTGAATTACCG
ATGAAGATCATGATGAACGTGGGTAACCCAGATCGCGCCTTCGACTTCGCTCGTTTACCAAACGAAGGTGTGGGTCTAGC
GCGTTTAGAATTCATCATCAACCGCATGATCGGTATTCACCCGAAAGCGCTGCTTGAATTCAACCAACAAGACGCTGCGC
TGCAGGAAGAAATCAACGAGATGATCGCAGGTTACGATTCTCCAGTTGAGTTCTACATTGCCCGCTTAGTCGAAGGTATC
GCCTCAATCGGTTCTGCTTTCTATCCGAAGAAAGTGATCGTGCGTATGTCAGACTTCAAGTCTAACGAATACGCTAACTT
AGTCGGTGGTGACCGTTACGAGCCAGAGGAAGAAAACCCAATGTTGGGCTTCCGTGGTGCGAGCCGTTATATCTCTGAAT
CATTCCGCGATTGTTTCGCGTTAGAATGTGAAGCGATTAAACGTGTTCGTAATGAGATGGGTCTGAAAAACGTCGAAGTG
ATGATCCCATTCGTACGTACTGTTAAAGAAGCCGAGCAAGTGATTGGACTGCTGAAAGAGCAGGGCTTAGAGCGCGGTAA
AGATGGTCTACGTGTCATCATGATGTGTGAAGTACCGTCAAACGCGCTGTTAGCGGATCAATTCCTTGAGCACTTCGATG
GTTTCTCTATCGGTTCAAACGATTTAACTCAGTTATCGCTCGGTCTTGACCGCGACTCTGGCATCATCAGCCACCTGTTC
GATGAGCGTAACGAAGCCGTTAAGATCCTACTGTCTATGGCCATTAAAGCGGCTAAGGCGAAGGGCGCTTACATCGGTAT
CTGTGGTCAAGGACCTTCAGATCACGCTGATTTCGCCGCCTGGTTAGTTGAGCAAGGCATCGATACTGTATCGTTAAACC
CAGATACCGTGATTGATACTTGGTTGTATTTAGCCGAAGCGCACGCTTGA

Upstream 100 bases:

>100_bases
TACAAATAAAATTATAGATTTACGCTGCTTCGGCGGGAGTGTAAAATGCCGCTGCCCTCGTGTGAAGGGGCCACTGAAAT
AAACTTGCGGAGATAGAACT

Downstream 100 bases:

>100_bases
TCTAGATTGTAATTTTATAAAGGTGTTAAAAAAGCCGCTTTTTAGCGGCTTTTTTTTGTTTATAATGGGCCGCATAACAA
TAATTGACTGAAGCTGCTCA

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 789; Mature: 789

Protein sequence:

>789_residues
MQQYVLWYQELGMGDVNLVGGKNASLGEMISNLANAGVQVPGGFATTSYAFNEFLEQSGVNQKIYDILATLDVDDVNALA
QVGAQIRQWVIDTPFQPALEQAIREAYDKLAAETSDASFAVRSSATAEDMPDASFAGQQETFLNVKGFDSVLVAIKHVFA
SLFNDRAISYRVHQGYEHHGVALSAGVQRMVRSDKAASGVMFTMDTESGNNDVVFITSSFGLGEMVVQGAVNPDEFYVHK
PILTQGHKAVVRRNIGSKLIQMVYSDDASHGKQVKIEDVAAEKRRQFSINDAEVMELAKQAMVIEKHYGRPMDIEWAKDG
NDGKLYIVQARPETVRSREDVQLIERYHLKSRGAVISEGRAIGHKVGSGVAKVLTSIADMDQIQPGDVLVTDMTDPDWEP
IMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDVTDRIKNGQLVTVSCAEGDTGYIYEGKQEFEVVSNRVDALPELP
MKIMMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNQQDAALQEEINEMIAGYDSPVEFYIARLVEGI
ASIGSAFYPKKVIVRMSDFKSNEYANLVGGDRYEPEEENPMLGFRGASRYISESFRDCFALECEAIKRVRNEMGLKNVEV
MIPFVRTVKEAEQVIGLLKEQGLERGKDGLRVIMMCEVPSNALLADQFLEHFDGFSIGSNDLTQLSLGLDRDSGIISHLF
DERNEAVKILLSMAIKAAKAKGAYIGICGQGPSDHADFAAWLVEQGIDTVSLNPDTVIDTWLYLAEAHA

Sequences:

>Translated_789_residues
MQQYVLWYQELGMGDVNLVGGKNASLGEMISNLANAGVQVPGGFATTSYAFNEFLEQSGVNQKIYDILATLDVDDVNALA
QVGAQIRQWVIDTPFQPALEQAIREAYDKLAAETSDASFAVRSSATAEDMPDASFAGQQETFLNVKGFDSVLVAIKHVFA
SLFNDRAISYRVHQGYEHHGVALSAGVQRMVRSDKAASGVMFTMDTESGNNDVVFITSSFGLGEMVVQGAVNPDEFYVHK
PILTQGHKAVVRRNIGSKLIQMVYSDDASHGKQVKIEDVAAEKRRQFSINDAEVMELAKQAMVIEKHYGRPMDIEWAKDG
NDGKLYIVQARPETVRSREDVQLIERYHLKSRGAVISEGRAIGHKVGSGVAKVLTSIADMDQIQPGDVLVTDMTDPDWEP
IMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDVTDRIKNGQLVTVSCAEGDTGYIYEGKQEFEVVSNRVDALPELP
MKIMMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNQQDAALQEEINEMIAGYDSPVEFYIARLVEGI
ASIGSAFYPKKVIVRMSDFKSNEYANLVGGDRYEPEEENPMLGFRGASRYISESFRDCFALECEAIKRVRNEMGLKNVEV
MIPFVRTVKEAEQVIGLLKEQGLERGKDGLRVIMMCEVPSNALLADQFLEHFDGFSIGSNDLTQLSLGLDRDSGIISHLF
DERNEAVKILLSMAIKAAKAKGAYIGICGQGPSDHADFAAWLVEQGIDTVSLNPDTVIDTWLYLAEAHA
>Mature_789_residues
MQQYVLWYQELGMGDVNLVGGKNASLGEMISNLANAGVQVPGGFATTSYAFNEFLEQSGVNQKIYDILATLDVDDVNALA
QVGAQIRQWVIDTPFQPALEQAIREAYDKLAAETSDASFAVRSSATAEDMPDASFAGQQETFLNVKGFDSVLVAIKHVFA
SLFNDRAISYRVHQGYEHHGVALSAGVQRMVRSDKAASGVMFTMDTESGNNDVVFITSSFGLGEMVVQGAVNPDEFYVHK
PILTQGHKAVVRRNIGSKLIQMVYSDDASHGKQVKIEDVAAEKRRQFSINDAEVMELAKQAMVIEKHYGRPMDIEWAKDG
NDGKLYIVQARPETVRSREDVQLIERYHLKSRGAVISEGRAIGHKVGSGVAKVLTSIADMDQIQPGDVLVTDMTDPDWEP
IMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDVTDRIKNGQLVTVSCAEGDTGYIYEGKQEFEVVSNRVDALPELP
MKIMMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNQQDAALQEEINEMIAGYDSPVEFYIARLVEGI
ASIGSAFYPKKVIVRMSDFKSNEYANLVGGDRYEPEEENPMLGFRGASRYISESFRDCFALECEAIKRVRNEMGLKNVEV
MIPFVRTVKEAEQVIGLLKEQGLERGKDGLRVIMMCEVPSNALLADQFLEHFDGFSIGSNDLTQLSLGLDRDSGIISHLF
DERNEAVKILLSMAIKAAKAKGAYIGICGQGPSDHADFAAWLVEQGIDTVSLNPDTVIDTWLYLAEAHA

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=782, Percent_Identity=74.9360613810742, Blast_Score=1258, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=457, Percent_Identity=28.0087527352298, Blast_Score=131, Evalue=2e-31,
Organism=Escherichia coli, GI1788726, Length=190, Percent_Identity=32.6315789473684, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI48994992, Length=402, Percent_Identity=26.6169154228856, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1789193, Length=403, Percent_Identity=25.3101736972705, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17564524, Length=329, Percent_Identity=26.4437689969605, Blast_Score=94, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 86723; Mature: 86723

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQYVLWYQELGMGDVNLVGGKNASLGEMISNLANAGVQVPGGFATTSYAFNEFLEQSGV
CCCCEEEHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
NQKIYDILATLDVDDVNALAQVGAQIRQWVIDTPFQPALEQAIREAYDKLAAETSDASFA
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEE
VRSSATAEDMPDASFAGQQETFLNVKGFDSVLVAIKHVFASLFNDRAISYRVHQGYEHHG
EECCCCCCCCCCCCCCCCCHHEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
VALSAGVQRMVRSDKAASGVMFTMDTESGNNDVVFITSSFGLGEMVVQGAVNPDEFYVHK
CHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHCCCCCCCEEEEC
PILTQGHKAVVRRNIGSKLIQMVYSDDASHGKQVKIEDVAAEKRRQFSINDAEVMELAKQ
CHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHH
AMVIEKHYGRPMDIEWAKDGNDGKLYIVQARPETVRSREDVQLIERYHLKSRGAVISEGR
HHHHHHHCCCCCCEEECCCCCCCEEEEEECCCHHHCCHHHHHHHHHHHHHHCCCEECCCC
AIGHKVGSGVAKVLTSIADMDQIQPGDVLVTDMTDPDWEPIMKRASAIVTNRGGRTCHAA
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHH
IIARELGVPAVVGCGDVTDRIKNGQLVTVSCAEGDTGYIYEGKQEFEVVSNRVDALPELP
HHHHHCCCCEEEECCHHHHHHCCCCEEEEEECCCCCCEEECCHHHHHHHHHHHHCCCCCC
MKIMMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNQQDAALQEEINE
HHHHEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHH
MIAGYDSPVEFYIARLVEGIASIGSAFYPKKVIVRMSDFKSNEYANLVGGDRYEPEEENP
HHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHEEEECCCCCCCCHHCCCCCCCCCCCCCC
MLGFRGASRYISESFRDCFALECEAIKRVRNEMGLKNVEVMIPFVRTVKEAEQVIGLLKE
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QGLERGKDGLRVIMMCEVPSNALLADQFLEHFDGFSIGSNDLTQLSLGLDRDSGIISHLF
HHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHEEEECCCCCCHHHHHHH
DERNEAVKILLSMAIKAAKAKGAYIGICGQGPSDHADFAAWLVEQGIDTVSLNPDTVIDT
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEECCCCHHHHH
WLYLAEAHA
HHHHHHCCC
>Mature Secondary Structure
MQQYVLWYQELGMGDVNLVGGKNASLGEMISNLANAGVQVPGGFATTSYAFNEFLEQSGV
CCCCEEEHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
NQKIYDILATLDVDDVNALAQVGAQIRQWVIDTPFQPALEQAIREAYDKLAAETSDASFA
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEE
VRSSATAEDMPDASFAGQQETFLNVKGFDSVLVAIKHVFASLFNDRAISYRVHQGYEHHG
EECCCCCCCCCCCCCCCCCHHEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
VALSAGVQRMVRSDKAASGVMFTMDTESGNNDVVFITSSFGLGEMVVQGAVNPDEFYVHK
CHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHCCCCCCCEEEEC
PILTQGHKAVVRRNIGSKLIQMVYSDDASHGKQVKIEDVAAEKRRQFSINDAEVMELAKQ
CHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHH
AMVIEKHYGRPMDIEWAKDGNDGKLYIVQARPETVRSREDVQLIERYHLKSRGAVISEGR
HHHHHHHCCCCCCEEECCCCCCCEEEEEECCCHHHCCHHHHHHHHHHHHHHCCCEECCCC
AIGHKVGSGVAKVLTSIADMDQIQPGDVLVTDMTDPDWEPIMKRASAIVTNRGGRTCHAA
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHH
IIARELGVPAVVGCGDVTDRIKNGQLVTVSCAEGDTGYIYEGKQEFEVVSNRVDALPELP
HHHHHCCCCEEEECCHHHHHHCCCCEEEEEECCCCCCEEECCHHHHHHHHHHHHCCCCCC
MKIMMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNQQDAALQEEINE
HHHHEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHH
MIAGYDSPVEFYIARLVEGIASIGSAFYPKKVIVRMSDFKSNEYANLVGGDRYEPEEENP
HHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHEEEECCCCCCCCHHCCCCCCCCCCCCCC
MLGFRGASRYISESFRDCFALECEAIKRVRNEMGLKNVEVMIPFVRTVKEAEQVIGLLKE
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QGLERGKDGLRVIMMCEVPSNALLADQFLEHFDGFSIGSNDLTQLSLGLDRDSGIISHLF
HHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHEEEECCCCCCHHHHHHH
DERNEAVKILLSMAIKAAKAKGAYIGICGQGPSDHADFAAWLVEQGIDTVSLNPDTVIDT
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEECCCCHHHHH
WLYLAEAHA
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1310524; 9097039; 9278503 [H]