Click here to switch to the map view.

The map label for this gene is rlmL [H]

Identifier: 113969882

GI number: 113969882

Start: 1816672

End: 1818813

Strand: Direct

Name: rlmL [H]

Synonym: Shewmr4_1540

Alternate gene names: 113969882

Gene position: 1816672-1818813 (Clockwise)

Preceding gene: 113969877

Following gene: 113969883

Centisome position: 38.6

GC content: 48.46

Gene sequence:

>2142_bases
ATGCTTAATTTTTTTGCTGCCGCCCCTAAGGGCTTTGAATATAGCTTAGCCCAAGAACTGACAGAATTTGGTGCGACTGA
GATTAAAGAAAGTGTTGCCGGTGTCTATTTTACCGCGCCGCTTGCCTTAGCTTACCGTATTACCCTGTGGACACGTTTGG
CCAGCCGTATCGTGCTGGTGATCTATAAGGGCCCCTGTGAGTCGGCGGAGCAATTATATAACGCAGCTTATTGTATTGAT
TGGTCATCCCATTTCTCCAATCGCAACACCTTTAGTATCGATTTTCACGGCACTGGCGGCTTTATCAACAACACCCAGTT
TGGTGCGTTAAAGATTAAAGATGCGATTGTTGACCGTTTCCGTGATGACGGTGATGCCCGTCCAAACGTCGCCCGTATCG
ATGCCGACATTAAAATCGACGCCCATTTCCGTAATGGGGTGATTACCATTGCGATGAACTTCTCGGGACCTTCCTTGCAT
CAACGCGGTTATCGCTCTACGACCGGTGAAGCCCCATTAAAAGAAAACCTCGCCGCCAACATGTTAGTACGCAGTGGCTG
GAAAGCCGCGCCAACCACCTTGCTCGATCCTTTCTGCGGTAGCGGTACTGTGCTGATTGAGGCCGCATTAATGGCTGCCG
ATATTGCCCCAGGATTGCAGCGCAGCCGTTTTGGTTTTGAACATTGGCGCCGCCACGATAAAGCCACCTGGCATGAGATT
TTAGAAGAGGCCAAGGCGCGCGCATCCTTAGGTGTAAAACGTTGTGACGTGAAGTTCTACGGCTCGGATATCGATTCGCG
CTTAGTGGCACTGGCAAAACGTAATGCGCAAAACGCCGGTGTACTTGAACTGATTGACTTTAAAGTGGCTAATGCGCTGA
ATGTCGAGCCGCCCGCTGGCGAAGGTTACTTGATTACTAACCCTCCCTACGGTGAACGTTTAGGCAGTGTCTCTGAGCTA
TTACAGCTGTATTACCAATTAGGCGACAAGTTTAAGAAGGAGTTTGGCGGCTGGAAAGTGGCCATGCTCTGTAGCGATAT
CGAGCTGATTTCGGCGCTTAAACTCAAAGCCGATAAGCAGATGAAGATGTTTAACGGTGCGCTAGAGTGTGCCTTTAACC
TTTATACCTTGCACGCGCAAAGTACTCGCCGTGATACGCCTGTATTGCCAGAAGGCGTGGATATTGCCGATATCGCACCA
GCTTTTGCGAACCGTATTAAGAAAAACGCTAAGCAATTAGAAAAGTGGGCGAAAAAAGAAGGTATCGACAGTTACCGTTT
ATATGATGCCGACATTCCCGAATATAACGTCGCGGTCGACCGCTATTTAGACCATATCGTGGTGCAGGAATATATGGCGC
CAGCCAGTATTCCAGAGGCCGTGACAAAACGCCGTTTAAGCGATGTGCTACTGGCTCTGCCTGCTGCTATTGGTGTCGAT
CCGCATAAGATCACTATGAAGACCCGTGAGCGTCAAAAGGGCACTAATCAATATCAAAAGCTGGATGAGCGTAAGCTTGA
ACTTATCACCACCGAATATGGCGCTAAGTTTAAGCTGAACCTGACGGGCTATTTGGATACAGGCTTGTTCCTTGACCACA
GATTAACCCGCCGTTTAGTGGGACAAAAATCGAAAGGGCGCCGGGTATTGAACCTGTTCTCTTACACTGGTTCCGCCTCT
GTGCATGCCGCATTAGGTGGCGCTAAGTCGGTCACCACGGTTGATATGTCAAACACCTATCTGGCGTGGGCGAAGGAAAA
CTTTGCGTTAAACGATCTCAGTGGCAAGCAGTATGAGTTTGTTCAGGCCGATTGTTTGCAATGGATCCGCGATAGTGCGC
TCGATAAATCCGCGCAATATGATTTGATTTTTATCGATCCGCCGACCTTCTCTAACTCCAAGCGGATGGAAGATTCCTTC
GATGTGCAACGTGACCATGTGAATTTACTCGGCATGCTCATCAAGCTGTTAAGTCCAAATGGGGAGATAGTGTTCTCCAA
CAACAAACGCAAGTTTAAGATGGACACTGAAACCTTGGCCAAGATGAAAATCAAGGTGGAAAACATCGATGATCTGACCT
TGCCTATGGATTACAAGCGCAATCCCCATATCCATAACACTTGGCTTATCACCCATGCATAA

Upstream 100 bases:

>100_bases
ACTGCCTGACTAAATCGAGTTTCGGCTAATAACTGTTTATCTTTTATGTCGCTTCACAGGTATAATGTCGGTTTGATTTT
GACTGCGTATTGATTATCCC

Downstream 100 bases:

>100_bases
TGTACTGCAAGCGGGTTACATTCTATACCACACCGAAGGGTGTCACTTATGTGAACTGGCCGCGGCATTGCTTAATGCCG
CCGAGGTGCCCTTTACGGCC

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 713; Mature: 713

Protein sequence:

>713_residues
MLNFFAAAPKGFEYSLAQELTEFGATEIKESVAGVYFTAPLALAYRITLWTRLASRIVLVIYKGPCESAEQLYNAAYCID
WSSHFSNRNTFSIDFHGTGGFINNTQFGALKIKDAIVDRFRDDGDARPNVARIDADIKIDAHFRNGVITIAMNFSGPSLH
QRGYRSTTGEAPLKENLAANMLVRSGWKAAPTTLLDPFCGSGTVLIEAALMAADIAPGLQRSRFGFEHWRRHDKATWHEI
LEEAKARASLGVKRCDVKFYGSDIDSRLVALAKRNAQNAGVLELIDFKVANALNVEPPAGEGYLITNPPYGERLGSVSEL
LQLYYQLGDKFKKEFGGWKVAMLCSDIELISALKLKADKQMKMFNGALECAFNLYTLHAQSTRRDTPVLPEGVDIADIAP
AFANRIKKNAKQLEKWAKKEGIDSYRLYDADIPEYNVAVDRYLDHIVVQEYMAPASIPEAVTKRRLSDVLLALPAAIGVD
PHKITMKTRERQKGTNQYQKLDERKLELITTEYGAKFKLNLTGYLDTGLFLDHRLTRRLVGQKSKGRRVLNLFSYTGSAS
VHAALGGAKSVTTVDMSNTYLAWAKENFALNDLSGKQYEFVQADCLQWIRDSALDKSAQYDLIFIDPPTFSNSKRMEDSF
DVQRDHVNLLGMLIKLLSPNGEIVFSNNKRKFKMDTETLAKMKIKVENIDDLTLPMDYKRNPHIHNTWLITHA

Sequences:

>Translated_713_residues
MLNFFAAAPKGFEYSLAQELTEFGATEIKESVAGVYFTAPLALAYRITLWTRLASRIVLVIYKGPCESAEQLYNAAYCID
WSSHFSNRNTFSIDFHGTGGFINNTQFGALKIKDAIVDRFRDDGDARPNVARIDADIKIDAHFRNGVITIAMNFSGPSLH
QRGYRSTTGEAPLKENLAANMLVRSGWKAAPTTLLDPFCGSGTVLIEAALMAADIAPGLQRSRFGFEHWRRHDKATWHEI
LEEAKARASLGVKRCDVKFYGSDIDSRLVALAKRNAQNAGVLELIDFKVANALNVEPPAGEGYLITNPPYGERLGSVSEL
LQLYYQLGDKFKKEFGGWKVAMLCSDIELISALKLKADKQMKMFNGALECAFNLYTLHAQSTRRDTPVLPEGVDIADIAP
AFANRIKKNAKQLEKWAKKEGIDSYRLYDADIPEYNVAVDRYLDHIVVQEYMAPASIPEAVTKRRLSDVLLALPAAIGVD
PHKITMKTRERQKGTNQYQKLDERKLELITTEYGAKFKLNLTGYLDTGLFLDHRLTRRLVGQKSKGRRVLNLFSYTGSAS
VHAALGGAKSVTTVDMSNTYLAWAKENFALNDLSGKQYEFVQADCLQWIRDSALDKSAQYDLIFIDPPTFSNSKRMEDSF
DVQRDHVNLLGMLIKLLSPNGEIVFSNNKRKFKMDTETLAKMKIKVENIDDLTLPMDYKRNPHIHNTWLITHA
>Mature_713_residues
MLNFFAAAPKGFEYSLAQELTEFGATEIKESVAGVYFTAPLALAYRITLWTRLASRIVLVIYKGPCESAEQLYNAAYCID
WSSHFSNRNTFSIDFHGTGGFINNTQFGALKIKDAIVDRFRDDGDARPNVARIDADIKIDAHFRNGVITIAMNFSGPSLH
QRGYRSTTGEAPLKENLAANMLVRSGWKAAPTTLLDPFCGSGTVLIEAALMAADIAPGLQRSRFGFEHWRRHDKATWHEI
LEEAKARASLGVKRCDVKFYGSDIDSRLVALAKRNAQNAGVLELIDFKVANALNVEPPAGEGYLITNPPYGERLGSVSEL
LQLYYQLGDKFKKEFGGWKVAMLCSDIELISALKLKADKQMKMFNGALECAFNLYTLHAQSTRRDTPVLPEGVDIADIAP
AFANRIKKNAKQLEKWAKKEGIDSYRLYDADIPEYNVAVDRYLDHIVVQEYMAPASIPEAVTKRRLSDVLLALPAAIGVD
PHKITMKTRERQKGTNQYQKLDERKLELITTEYGAKFKLNLTGYLDTGLFLDHRLTRRLVGQKSKGRRVLNLFSYTGSAS
VHAALGGAKSVTTVDMSNTYLAWAKENFALNDLSGKQYEFVQADCLQWIRDSALDKSAQYDLIFIDPPTFSNSKRMEDSF
DVQRDHVNLLGMLIKLLSPNGEIVFSNNKRKFKMDTETLAKMKIKVENIDDLTLPMDYKRNPHIHNTWLITHA

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=716, Percent_Identity=49.5810055865922, Blast_Score=690, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=274, Percent_Identity=28.8321167883212, Blast_Score=102, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 80033; Mature: 80033

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNFFAAAPKGFEYSLAQELTEFGATEIKESVAGVYFTAPLALAYRITLWTRLASRIVLV
CCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCEEEE
IYKGPCESAEQLYNAAYCIDWSSHFSNRNTFSIDFHGTGGFINNTQFGALKIKDAIVDRF
EECCCCHHHHHHHCEEEEEEHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEHHHHHHHHH
RDDGDARPNVARIDADIKIDAHFRNGVITIAMNFSGPSLHQRGYRSTTGEAPLKENLAAN
CCCCCCCCCEEEECCEEEEEEEECCCEEEEEEECCCCCHHHCCCCCCCCCCCHHHHHHHH
MLVRSGWKAAPTTLLDPFCGSGTVLIEAALMAADIAPGLQRSRFGFEHWRRHDKATWHEI
HHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCHHHHHHCCCHHHHHH
LEEAKARASLGVKRCDVKFYGSDIDSRLVALAKRNAQNAGVLELIDFKVANALNVEPPAG
HHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEEEEHHHHCCCCCCCCCC
EGYLITNPPYGERLGSVSELLQLYYQLGDKFKKEFGGWKVAMLCSDIELISALKLKADKQ
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHCCCHH
MKMFNGALECAFNLYTLHAQSTRRDTPVLPEGVDIADIAPAFANRIKKNAKQLEKWAKKE
HHHHHHHHHHEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GIDSYRLYDADIPEYNVAVDRYLDHIVVQEYMAPASIPEAVTKRRLSDVLLALPAAIGVD
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PHKITMKTRERQKGTNQYQKLDERKLELITTEYGAKFKLNLTGYLDTGLFLDHRLTRRLV
CCEEEEHHHHHHCCCHHHHHHHHHHHEEEEECCCCEEEEEEEEEECCCHHHHHHHHHHHH
GQKSKGRRVLNLFSYTGSASVHAALGGAKSVTTVDMSNTYLAWAKENFALNDLSGKQYEF
CCCCCCCEEEEEEECCCCCCEEEECCCCCCEEEEECCCCEEEEECCCCEEECCCCCCCHH
VQADCLQWIRDSALDKSAQYDLIFIDPPTFSNSKRMEDSFDVQRDHVNLLGMLIKLLSPN
HHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
GEIVFSNNKRKFKMDTETLAKMKIKVENIDDLTLPMDYKRNPHIHNTWLITHA
CCEEECCCCEEEEECHHHHEEEEEEEECCCCEECCCCCCCCCCCCEEEEEEEC
>Mature Secondary Structure
MLNFFAAAPKGFEYSLAQELTEFGATEIKESVAGVYFTAPLALAYRITLWTRLASRIVLV
CCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCEEEE
IYKGPCESAEQLYNAAYCIDWSSHFSNRNTFSIDFHGTGGFINNTQFGALKIKDAIVDRF
EECCCCHHHHHHHCEEEEEEHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEHHHHHHHHH
RDDGDARPNVARIDADIKIDAHFRNGVITIAMNFSGPSLHQRGYRSTTGEAPLKENLAAN
CCCCCCCCCEEEECCEEEEEEEECCCEEEEEEECCCCCHHHCCCCCCCCCCCHHHHHHHH
MLVRSGWKAAPTTLLDPFCGSGTVLIEAALMAADIAPGLQRSRFGFEHWRRHDKATWHEI
HHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCHHHHHHCCCHHHHHH
LEEAKARASLGVKRCDVKFYGSDIDSRLVALAKRNAQNAGVLELIDFKVANALNVEPPAG
HHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEEEEHHHHCCCCCCCCCC
EGYLITNPPYGERLGSVSELLQLYYQLGDKFKKEFGGWKVAMLCSDIELISALKLKADKQ
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHCCCHH
MKMFNGALECAFNLYTLHAQSTRRDTPVLPEGVDIADIAPAFANRIKKNAKQLEKWAKKE
HHHHHHHHHHEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GIDSYRLYDADIPEYNVAVDRYLDHIVVQEYMAPASIPEAVTKRRLSDVLLALPAAIGVD
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PHKITMKTRERQKGTNQYQKLDERKLELITTEYGAKFKLNLTGYLDTGLFLDHRLTRRLV
CCEEEEHHHHHHCCCHHHHHHHHHHHEEEEECCCCEEEEEEEEEECCCHHHHHHHHHHHH
GQKSKGRRVLNLFSYTGSASVHAALGGAKSVTTVDMSNTYLAWAKENFALNDLSGKQYEF
CCCCCCCEEEEEEECCCCCCEEEECCCCCCEEEEECCCCEEEEECCCCEEECCCCCCCHH
VQADCLQWIRDSALDKSAQYDLIFIDPPTFSNSKRMEDSFDVQRDHVNLLGMLIKLLSPN
HHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
GEIVFSNNKRKFKMDTETLAKMKIKVENIDDLTLPMDYKRNPHIHNTWLITHA
CCEEECCCCEEEEECHHHHEEEEEEEECCCCEECCCCCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA