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The map label for this gene is folC [H]

Identifier: 113969769

GI number: 113969769

Start: 1668361

End: 1669659

Strand: Direct

Name: folC [H]

Synonym: Shewmr4_1427

Alternate gene names: 113969769

Gene position: 1668361-1669659 (Clockwise)

Preceding gene: 113969768

Following gene: 113969770

Centisome position: 35.45

GC content: 52.19

Gene sequence:

>1299_bases
ATGACCACAGCCCCGACTGAACAGACAGCCTTAACTGTGCCGAGTGCGAATGCGCCATTGGCACAATGGCTAGATTATTT
ATTATCGATTCACCCAACCGAAATCGATATGGGATTAACCCGGGTTTCTAAGGTTGCCACGCGCCTGCATTTATTGGATT
TGGGGCAAAGTAAAGTGGTCACTGTGGCGGGCACCAATGGCAAAGGCACCACCTGCGCCATGCTGGAAAACATCCTACGT
CTATCTGGGCTGACAGTGGGCGTTTACAGCTCGCCCCATATGCTCAAGTACAACGAAAGGGTGCGCATCAACGGTCAAGA
TGCCAGCGATGAAGCCTTTGTGGCGGCCTTTGAACAGATTGAAGCTGCGCGCGGCGAGATTTCGCTGACTTTCTTCGAAT
ATGCCACCTTGGCGGGCTTGATCCTGTTTAAAGCGGCCAAGTTGGATGTGGTGATATTAGAAGTCGGTCTTGGCGGCCGT
TTAGATGCCACCAACATGATTGATGCCGATATCAGCGTGGTGACGTCCGTCGATTTAGACCACGAGGCGTATTTGGGTAA
TACCCGCGAGCTGGTCGGCCGTGAAAAGGCGGGTATTTTTCGCCAAGGTCGCCCTGCGATTGTCGGTGAGCCGAACTTAC
CCCATACGGTCAATGATGTCGCCCAAGATGTTGGCGCCAAGCTCTATCGTGTGGGCGAGGAGTTTAGTTACCAGCAAGAT
GGCCAGACGTGGGATTACCAAGGCCAACTATTCACCCTTAAGCAGCTGAGTTTGCCGACACTGCCATTACCGAACGCCGC
GACTGTGATTGCGATTGTTGAGCATGGCTGGCCGAATATTGCAGCCGATATCATAGCCAAAGGCATCAGCAGCGCGCGCT
TAACCGGGCGTTTAGAACAAGTTAACGAGCAGCCGCTGATTTTACTCGATGTGGCGCACAATCCCCATGCGGCGCGCTTT
TTAGTCAAACAGCTCACGCCTATGGTGACGGGGAAGCGAGTGTTTGCCCTGTGCGGCATGTTAAAGGATAAGGATATTGC
AGGCGTATTGCCTGTGGTTTCGCCCTTAATCGATACTTGGTACTTAGTGAGTTTACAGGGTGAGCGCGGCGCGAATGCAA
GCCTGCTGCGCCAAACCTTAGCGCAAGACACTAAGGCCCATGAGTTTGACGATATCGCCTCGGCATGGACGGCATTAAAA
GCGCAAATTCAGCCCGATGATGTGGTAATTGTGTTTGGCTCCTTTTACACTGTGGCAGGTTTTAAGTCTCTCTTTAAGCA
GGATAACCGTTTTGTCTAG

Upstream 100 bases:

>100_bases
GTATCAACTGCCGAAGTTGGCTTTAGGGCCATTGTTTATGCTGGATTAATCGCCGTTTGGCCGATTAAGTGAAGCGCGTA
TCGAAGGAAGGAAAGAACAG

Downstream 100 bases:

>100_bases
CCAGTTTCATAATCGATTAGTCGGCACTGTGGTGCTGGTCGCCCTCGGGGTGATTTTTCTGCCGGATATTTTAGATGGCA
AGAAGGACCGCCAAGAGGAA

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTTAPTEQTALTVPSANAPLAQWLDYLLSIHPTEIDMGLTRVSKVATRLHLLDLGQSKVVTVAGTNGKGTTCAMLENILR
LSGLTVGVYSSPHMLKYNERVRINGQDASDEAFVAAFEQIEAARGEISLTFFEYATLAGLILFKAAKLDVVILEVGLGGR
LDATNMIDADISVVTSVDLDHEAYLGNTRELVGREKAGIFRQGRPAIVGEPNLPHTVNDVAQDVGAKLYRVGEEFSYQQD
GQTWDYQGQLFTLKQLSLPTLPLPNAATVIAIVEHGWPNIAADIIAKGISSARLTGRLEQVNEQPLILLDVAHNPHAARF
LVKQLTPMVTGKRVFALCGMLKDKDIAGVLPVVSPLIDTWYLVSLQGERGANASLLRQTLAQDTKAHEFDDIASAWTALK
AQIQPDDVVIVFGSFYTVAGFKSLFKQDNRFV

Sequences:

>Translated_432_residues
MTTAPTEQTALTVPSANAPLAQWLDYLLSIHPTEIDMGLTRVSKVATRLHLLDLGQSKVVTVAGTNGKGTTCAMLENILR
LSGLTVGVYSSPHMLKYNERVRINGQDASDEAFVAAFEQIEAARGEISLTFFEYATLAGLILFKAAKLDVVILEVGLGGR
LDATNMIDADISVVTSVDLDHEAYLGNTRELVGREKAGIFRQGRPAIVGEPNLPHTVNDVAQDVGAKLYRVGEEFSYQQD
GQTWDYQGQLFTLKQLSLPTLPLPNAATVIAIVEHGWPNIAADIIAKGISSARLTGRLEQVNEQPLILLDVAHNPHAARF
LVKQLTPMVTGKRVFALCGMLKDKDIAGVLPVVSPLIDTWYLVSLQGERGANASLLRQTLAQDTKAHEFDDIASAWTALK
AQIQPDDVVIVFGSFYTVAGFKSLFKQDNRFV
>Mature_431_residues
TTAPTEQTALTVPSANAPLAQWLDYLLSIHPTEIDMGLTRVSKVATRLHLLDLGQSKVVTVAGTNGKGTTCAMLENILRL
SGLTVGVYSSPHMLKYNERVRINGQDASDEAFVAAFEQIEAARGEISLTFFEYATLAGLILFKAAKLDVVILEVGLGGRL
DATNMIDADISVVTSVDLDHEAYLGNTRELVGREKAGIFRQGRPAIVGEPNLPHTVNDVAQDVGAKLYRVGEEFSYQQDG
QTWDYQGQLFTLKQLSLPTLPLPNAATVIAIVEHGWPNIAADIIAKGISSARLTGRLEQVNEQPLILLDVAHNPHAARFL
VKQLTPMVTGKRVFALCGMLKDKDIAGVLPVVSPLIDTWYLVSLQGERGANASLLRQTLAQDTKAHEFDDIASAWTALKA
QIQPDDVVIVFGSFYTVAGFKSLFKQDNRFV

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=191, Percent_Identity=40.3141361256544, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI66932990, Length=191, Percent_Identity=39.2670157068063, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1788654, Length=406, Percent_Identity=52.2167487684729, Blast_Score=404, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17553148, Length=160, Percent_Identity=37.5, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17553150, Length=160, Percent_Identity=37.5, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI71984923, Length=160, Percent_Identity=37.5, Blast_Score=121, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6323760, Length=315, Percent_Identity=30.1587301587302, Blast_Score=123, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6324815, Length=181, Percent_Identity=32.0441988950276, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24641571, Length=185, Percent_Identity=36.2162162162162, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24581568, Length=187, Percent_Identity=33.6898395721925, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 46977; Mature: 46846

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAPTEQTALTVPSANAPLAQWLDYLLSIHPTEIDMGLTRVSKVATRLHLLDLGQSKVV
CCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCEEE
TVAGTNGKGTTCAMLENILRLSGLTVGVYSSPHMLKYNERVRINGQDASDEAFVAAFEQI
EEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEECCEEEECCCCCCCHHHHHHHHHH
EAARGEISLTFFEYATLAGLILFKAAKLDVVILEVGLGGRLDATNMIDADISVVTSVDLD
HHCCCCEEEEHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEECCCEEEEEEECCC
HEAYLGNTRELVGREKAGIFRQGRPAIVGEPNLPHTVNDVAQDVGAKLYRVGEEFSYQQD
CHHHCCCHHHHHCCHHCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
GQTWDYQGQLFTLKQLSLPTLPLPNAATVIAIVEHGWPNIAADIIAKGISSARLTGRLEQ
CCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHH
VNEQPLILLDVAHNPHAARFLVKQLTPMVTGKRVFALCGMLKDKDIAGVLPVVSPLIDTW
CCCCCEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCE
YLVSLQGERGANASLLRQTLAQDTKAHEFDDIASAWTALKAQIQPDDVVIVFGSFYTVAG
EEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEECCCCEEEEECCHHHHHH
FKSLFKQDNRFV
HHHHHHCCCCCC
>Mature Secondary Structure 
TTAPTEQTALTVPSANAPLAQWLDYLLSIHPTEIDMGLTRVSKVATRLHLLDLGQSKVV
CCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCEEE
TVAGTNGKGTTCAMLENILRLSGLTVGVYSSPHMLKYNERVRINGQDASDEAFVAAFEQI
EEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEECCEEEECCCCCCCHHHHHHHHHH
EAARGEISLTFFEYATLAGLILFKAAKLDVVILEVGLGGRLDATNMIDADISVVTSVDLD
HHCCCCEEEEHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEECCCEEEEEEECCC
HEAYLGNTRELVGREKAGIFRQGRPAIVGEPNLPHTVNDVAQDVGAKLYRVGEEFSYQQD
CHHHCCCHHHHHCCHHCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
GQTWDYQGQLFTLKQLSLPTLPLPNAATVIAIVEHGWPNIAADIIAKGISSARLTGRLEQ
CCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHH
VNEQPLILLDVAHNPHAARFLVKQLTPMVTGKRVFALCGMLKDKDIAGVLPVVSPLIDTW
CCCCCEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCE
YLVSLQGERGANASLLRQTLAQDTKAHEFDDIASAWTALKAQIQPDDVVIVFGSFYTVAG
EEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEECCCCEEEEECCHHHHHH
FKSLFKQDNRFV
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]