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The map label for this gene is pdxB [H]

Identifier: 113969765

GI number: 113969765

Start: 1661702

End: 1662832

Strand: Direct

Name: pdxB [H]

Synonym: Shewmr4_1423

Alternate gene names: 113969765

Gene position: 1661702-1662832 (Clockwise)

Preceding gene: 113969764

Following gene: 113969766

Centisome position: 35.31

GC content: 50.13

Gene sequence:

>1131_bases
ATGAAGATTGTTGTCGATGAAAATATGCCCTATGTCGAACCCTTATTTGGGGCGTTAGGCGAGATAATCCCAGTCAATGG
CCGCACGCTAACGCCCGAGCAGGTGCAAGATGCCGATGTGCTACTGGTGCGCTCGGTCACTCGAGTCAATGCCGCATTGT
TAGATGCCAATCCAAAGCTTAAGTTTGTCGGCAGCGCCACTATAGGGACGGATCATGTGGATCTGGCCTACCTTGCAAGG
CGTGGTATTCCCTTTTCGAATGCACCCGGCTGTAATGCGACCGCAGTCGGGGAGTTTGCCTTTATTGCGATGTTAGAACT
GGCGGCGCGGTTTAACTCGCCGCTCAAGGGCAAAGTTGTGGGGATTGTCGGCGCGGGCAATACTGGCAGCGCGACGGCCA
AATGCCTCGAAGCCTACGGCATTAAGGTATTACTCAATGACCCCATCAAAGCGGCCGAGGGCGATCCCCGTCATTTTGTA
AGCCTTGAGACTCTATTACACCAAGCGGATATCATCAGCCTACATGTGCCCATCACTCGCAGTGGCGAGCATAAGACGCT
GCATTTATTCGATGAAGCACGCCTGATGAGTCTTAAACCCAATACCTGGCTGGTGAACTGTTGCCGTGGTGATGTAATTG
ATAATCAAGCCTTAATTAAGGTTAAGCAGCAAAGGGATGACCTTAAATTGGTGCTCGATGTATGGGAAGGGGAGCCGCAT
CCCATGCCTGAGCTGGTACCTTTTGCTGAGTTTGCGACTCCGCATATCGCCGGTTACAGCTTAGAAGGTAAGGCCCGTGG
CACTTTTATGCTGTACCAAAAGTTGTGTGAGTTGTTAGCCATTCCGGCCAACAAACGCTTATCCGAGCTCTTGCCGCCAT
TTCATTTTAAGGCGGTCGAACTCGAACAAACTCCCGATGAAAAAGCCTTATTACAGCTCGCAAGATTCGTCTACGACCTG
CGTGATGACGATGCCGTATTCCGAAATGGCTTTGCAAAGGAGAATGGCTTCGACATTATGAGAAAAAATCACAAACATAG
ACGAGAATTTAGTGCATTGGCGCTGGCTTATCACGGCCAATCTGAGGTAGATTGGTTGTCCAATTTAGGTTTTTCAGGAG
TCGGTCGGTAA

Upstream 100 bases:

>100_bases
AATACGATTGGTATAATGCGACTCAGGGATTGGGATTTATCTCTAGAGTCTTGTTAGAATCCTGAATATCTTGATATTGC
TGTTTATCAGAAAGGTCCCC

Downstream 100 bases:

>100_bases
TTATGTCGCAGGAATTTAATGTTGTCGTTTTAGGTGCATCGGGCGCAGTGGGTCAAACTATGATTGAGATCCTCGAAGAG
CGTAACTTCCCGGTAGCGAA

Product: D-isomer specific 2-hydroxyacid dehydrogenase, catalytic region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKIVVDENMPYVEPLFGALGEIIPVNGRTLTPEQVQDADVLLVRSVTRVNAALLDANPKLKFVGSATIGTDHVDLAYLAR
RGIPFSNAPGCNATAVGEFAFIAMLELAARFNSPLKGKVVGIVGAGNTGSATAKCLEAYGIKVLLNDPIKAAEGDPRHFV
SLETLLHQADIISLHVPITRSGEHKTLHLFDEARLMSLKPNTWLVNCCRGDVIDNQALIKVKQQRDDLKLVLDVWEGEPH
PMPELVPFAEFATPHIAGYSLEGKARGTFMLYQKLCELLAIPANKRLSELLPPFHFKAVELEQTPDEKALLQLARFVYDL
RDDDAVFRNGFAKENGFDIMRKNHKHRREFSALALAYHGQSEVDWLSNLGFSGVGR

Sequences:

>Translated_376_residues
MKIVVDENMPYVEPLFGALGEIIPVNGRTLTPEQVQDADVLLVRSVTRVNAALLDANPKLKFVGSATIGTDHVDLAYLAR
RGIPFSNAPGCNATAVGEFAFIAMLELAARFNSPLKGKVVGIVGAGNTGSATAKCLEAYGIKVLLNDPIKAAEGDPRHFV
SLETLLHQADIISLHVPITRSGEHKTLHLFDEARLMSLKPNTWLVNCCRGDVIDNQALIKVKQQRDDLKLVLDVWEGEPH
PMPELVPFAEFATPHIAGYSLEGKARGTFMLYQKLCELLAIPANKRLSELLPPFHFKAVELEQTPDEKALLQLARFVYDL
RDDDAVFRNGFAKENGFDIMRKNHKHRREFSALALAYHGQSEVDWLSNLGFSGVGR
>Mature_376_residues
MKIVVDENMPYVEPLFGALGEIIPVNGRTLTPEQVQDADVLLVRSVTRVNAALLDANPKLKFVGSATIGTDHVDLAYLAR
RGIPFSNAPGCNATAVGEFAFIAMLELAARFNSPLKGKVVGIVGAGNTGSATAKCLEAYGIKVLLNDPIKAAEGDPRHFV
SLETLLHQADIISLHVPITRSGEHKTLHLFDEARLMSLKPNTWLVNCCRGDVIDNQALIKVKQQRDDLKLVLDVWEGEPH
PMPELVPFAEFATPHIAGYSLEGKARGTFMLYQKLCELLAIPANKRLSELLPPFHFKAVELEQTPDEKALLQLARFVYDL
RDDDAVFRNGFAKENGFDIMRKNHKHRREFSALALAYHGQSEVDWLSNLGFSGVGR

Specific function: Catalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=255, Percent_Identity=29.4117647058824, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI145580578, Length=226, Percent_Identity=26.5486725663717, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI4557499, Length=226, Percent_Identity=26.5486725663717, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI145580575, Length=159, Percent_Identity=32.0754716981132, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI61743967, Length=235, Percent_Identity=25.1063829787234, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI4557497, Length=235, Percent_Identity=25.1063829787234, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1788660, Length=374, Percent_Identity=47.3262032085562, Blast_Score=302, Evalue=3e-83,
Organism=Escherichia coli, GI1789279, Length=257, Percent_Identity=29.9610894941634, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787645, Length=199, Percent_Identity=28.643216080402, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082289, Length=268, Percent_Identity=27.9850746268657, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17532191, Length=252, Percent_Identity=28.968253968254, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320925, Length=266, Percent_Identity=28.1954887218045, Blast_Score=106, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6322116, Length=266, Percent_Identity=28.1954887218045, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324055, Length=225, Percent_Identity=25.3333333333333, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24646446, Length=239, Percent_Identity=28.4518828451883, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24646448, Length=239, Percent_Identity=28.4518828451883, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24646452, Length=239, Percent_Identity=28.4518828451883, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24646450, Length=239, Percent_Identity=28.4518828451883, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI62472511, Length=239, Percent_Identity=28.4518828451883, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19921140, Length=265, Percent_Identity=26.0377358490566, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28574286, Length=266, Percent_Identity=26.6917293233083, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28574284, Length=272, Percent_Identity=25.7352941176471, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24585514, Length=272, Percent_Identity=25.7352941176471, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28574282, Length=272, Percent_Identity=25.7352941176471, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45552429, Length=272, Percent_Identity=25.7352941176471, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45551003, Length=272, Percent_Identity=25.7352941176471, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28571528, Length=287, Percent_Identity=25.7839721254355, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR020921
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.290 [H]

Molecular weight: Translated: 41483; Mature: 41483

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVVDENMPYVEPLFGALGEIIPVNGRTLTPEQVQDADVLLVRSVTRVNAALLDANPKL
CEEEEECCCCHHHHHHHHHCCEEECCCCEECHHHHCCCCEEEHHHHHHHHEEEECCCCCE
KFVGSATIGTDHVDLAYLARRGIPFSNAPGCNATAVGEFAFIAMLELAARFNSPLKGKVV
EEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
GIVGAGNTGSATAKCLEAYGIKVLLNDPIKAAEGDPRHFVSLETLLHQADIISLHVPITR
EEEECCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEEEEC
SGEHKTLHLFDEARLMSLKPNTWLVNCCRGDVIDNQALIKVKQQRDDLKLVLDVWEGEPH
CCCCCEEEEECCCEEEEECCCCEEEEECCCCCCCCHHHEEECCCCCCEEEEEEEECCCCC
PMPELVPFAEFATPHIAGYSLEGKARGTFMLYQKLCELLAIPANKRLSELLPPFHFKAVE
CCCCCCCHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCEEEEE
LEQTPDEKALLQLARFVYDLRDDDAVFRNGFAKENGFDIMRKNHKHRREFSALALAYHGQ
ECCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHCCHHHHHHHHHEEEEECCC
SEVDWLSNLGFSGVGR
CHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKIVVDENMPYVEPLFGALGEIIPVNGRTLTPEQVQDADVLLVRSVTRVNAALLDANPKL
CEEEEECCCCHHHHHHHHHCCEEECCCCEECHHHHCCCCEEEHHHHHHHHEEEECCCCCE
KFVGSATIGTDHVDLAYLARRGIPFSNAPGCNATAVGEFAFIAMLELAARFNSPLKGKVV
EEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
GIVGAGNTGSATAKCLEAYGIKVLLNDPIKAAEGDPRHFVSLETLLHQADIISLHVPITR
EEEECCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEEEEC
SGEHKTLHLFDEARLMSLKPNTWLVNCCRGDVIDNQALIKVKQQRDDLKLVLDVWEGEPH
CCCCCEEEEECCCEEEEECCCCEEEEECCCCCCCCHHHEEECCCCCCEEEEEEEECCCCC
PMPELVPFAEFATPHIAGYSLEGKARGTFMLYQKLCELLAIPANKRLSELLPPFHFKAVE
CCCCCCCHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCEEEEE
LEQTPDEKALLQLARFVYDLRDDDAVFRNGFAKENGFDIMRKNHKHRREFSALALAYHGQ
ECCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHCCHHHHHHHHHEEEEECCC
SEVDWLSNLGFSGVGR
CHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]