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The map label for this gene is rfbB [H]

Identifier: 113969661

GI number: 113969661

Start: 1527742

End: 1528821

Strand: Direct

Name: rfbB [H]

Synonym: Shewmr4_1318

Alternate gene names: 113969661

Gene position: 1527742-1528821 (Clockwise)

Preceding gene: 113969660

Following gene: 113969662

Centisome position: 32.46

GC content: 43.15

Gene sequence:

>1080_bases
ATGAAAATTCTAGTTACAGGTGGAGCAGGTTTTATTGGCTCTGCAGTGGTTCGTCATATTATCAGCAATACTCAAGATAG
CGTAATTAACCTTGATAAACTGACATATGCAGGCAATTTAGAATCGCTTGTAAGCGTTGAGGCTAGTGAGCGTTATGCAT
TTGAGCAAGTCGATATTTGTGACCGTGCTGAGCTGGATAGAGTCTTTTCACAATATAAACCTGATGCAGTAATGCATTTG
GCGGCAGAGTCACATGTTGACCGTTCAATTACCGGTCCGGCTGATTTTATTCAAACGAATATAGTAGGGACTTACACACT
ACTCGAAGCTGCTCGCCATTACTGGATGCAACTTGATGCTGAACGCAAAGTAGCATTTCGCTTTCACCATATCTCAACGG
ATGAAGTGTATGGTGATTTGCCACATCCTGATGAACAAGAAGGCCAAGTGGTCAATCAAGAGCTGCCTTTATTTACCGAG
ACCACGCCTTATGCACCAAGCAGCCCTTATTCTGCCTCTAAAGCTTCCAGTGACCATTTAGTTCGCGCTTGGCTGCGTAC
CTATGGCTTACCAACGATTGTAACCAACTGCTCAAATAACTACGGTCCTTATCATTTTCCAGAAAAGTTGATCCCGCTGG
TTATCCTCAATGCCCTTGAAGGCAAGCAATTACCCATTTACGGCAAAGGCGACCAGATCCGTGATTGGCTGTATGTTGAA
GACCATGCTCGTGCGCTATACAAAGTGGTTACAGAAGGTCAAATTGGCGAAACTTACAATATCGGCGGACATAACGAGAA
ACAAAATTTAGAAGTAGTGCAGACTATTTGCACTATTCTTGATGCTCTCGTTCCAAAGGCAAGTTCGTATGCAGAGCAAA
TAACTTATGTAACTGATAGACCTGGCCACGATCGCCGTTATGCCATTGATGCTTCTAAAATCAGCAATGAGCTAAATTGG
CAACCACAAGAAACCTTTGAAACTGGCCTGTGTAAAACAGTTGAATGGTATTTAGCAAATCAAGAATGGTGTCAGCATGT
TCAAGATGGCACCTATCAGCGAGAGCGTTTAGGCATTTAA

Upstream 100 bases:

>100_bases
CTGGTTTGTAAAGCTGAGATGGAAAAGTTAAAACGCTCATTATTTATCACTCAAATATAGCCATTTTATATAGTTAAACT
CATCGGATTTGGTAGTAAAA

Downstream 100 bases:

>100_bases
TCTCTTTAACAACTTTATTTTAAAGTCCTAGTTTTTACCTAGGGCCTATTTACCTAGGAACTTGATTTAAATGAAAGGAA
TTATTTTAGCTGGCGGCTCA

Product: dTDP-glucose 4,6 dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MKILVTGGAGFIGSAVVRHIISNTQDSVINLDKLTYAGNLESLVSVEASERYAFEQVDICDRAELDRVFSQYKPDAVMHL
AAESHVDRSITGPADFIQTNIVGTYTLLEAARHYWMQLDAERKVAFRFHHISTDEVYGDLPHPDEQEGQVVNQELPLFTE
TTPYAPSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKQLPIYGKGDQIRDWLYVE
DHARALYKVVTEGQIGETYNIGGHNEKQNLEVVQTICTILDALVPKASSYAEQITYVTDRPGHDRRYAIDASKISNELNW
QPQETFETGLCKTVEWYLANQEWCQHVQDGTYQRERLGI

Sequences:

>Translated_359_residues
MKILVTGGAGFIGSAVVRHIISNTQDSVINLDKLTYAGNLESLVSVEASERYAFEQVDICDRAELDRVFSQYKPDAVMHL
AAESHVDRSITGPADFIQTNIVGTYTLLEAARHYWMQLDAERKVAFRFHHISTDEVYGDLPHPDEQEGQVVNQELPLFTE
TTPYAPSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKQLPIYGKGDQIRDWLYVE
DHARALYKVVTEGQIGETYNIGGHNEKQNLEVVQTICTILDALVPKASSYAEQITYVTDRPGHDRRYAIDASKISNELNW
QPQETFETGLCKTVEWYLANQEWCQHVQDGTYQRERLGI
>Mature_359_residues
MKILVTGGAGFIGSAVVRHIISNTQDSVINLDKLTYAGNLESLVSVEASERYAFEQVDICDRAELDRVFSQYKPDAVMHL
AAESHVDRSITGPADFIQTNIVGTYTLLEAARHYWMQLDAERKVAFRFHHISTDEVYGDLPHPDEQEGQVVNQELPLFTE
TTPYAPSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKQLPIYGKGDQIRDWLYVE
DHARALYKVVTEGQIGETYNIGGHNEKQNLEVVQTICTILDALVPKASSYAEQITYVTDRPGHDRRYAIDASKISNELNW
QPQETFETGLCKTVEWYLANQEWCQHVQDGTYQRERLGI

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=343, Percent_Identity=36.1516034985423, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI42516563, Length=345, Percent_Identity=23.1884057971014, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI56237023, Length=348, Percent_Identity=28.1609195402299, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI56118217, Length=348, Percent_Identity=28.1609195402299, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI189083684, Length=348, Percent_Identity=28.1609195402299, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1788353, Length=353, Percent_Identity=78.7535410764873, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI48994969, Length=362, Percent_Identity=71.8232044198895, Blast_Score=546, Evalue=1e-157,
Organism=Escherichia coli, GI1786974, Length=362, Percent_Identity=25.9668508287293, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788366, Length=201, Percent_Identity=29.8507462686567, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788365, Length=356, Percent_Identity=21.6292134831461, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17568069, Length=348, Percent_Identity=36.4942528735632, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI115532424, Length=334, Percent_Identity=33.2335329341317, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI71982035, Length=378, Percent_Identity=28.042328042328, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71982038, Length=380, Percent_Identity=27.8947368421053, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17539532, Length=345, Percent_Identity=25.7971014492754, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI133901790, Length=257, Percent_Identity=25.2918287937743, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=373, Percent_Identity=27.0777479892761, Blast_Score=96, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21356223, Length=341, Percent_Identity=25.5131964809384, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19923002, Length=364, Percent_Identity=26.6483516483516, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24158427, Length=286, Percent_Identity=25.5244755244755, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40572; Mature: 40572

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGGAGFIGSAVVRHIISNTQDSVINLDKLTYAGNLESLVSVEASERYAFEQVDIC
CEEEEECCCCHHHHHHHHHHHCCCCCCEEEHHHHEECCCHHHHHHHHHHHHHHHHHCCCH
DRAELDRVFSQYKPDAVMHLAAESHVDRSITGPADFIQTNIVGTYTLLEAARHYWMQLDA
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
ERKVAFRFHHISTDEVYGDLPHPDEQEGQVVNQELPLFTETTPYAPSSPYSASKASSDHL
CCEEEEEEEECCHHHHCCCCCCCCCCCCCEECCCCCCEECCCCCCCCCCCCCCCCCCHHH
VRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKQLPIYGKGDQIRDWLYVE
HHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCEEEEH
DHARALYKVVTEGQIGETYNIGGHNEKQNLEVVQTICTILDALVPKASSYAEQITYVTDR
HHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEECC
PGHDRRYAIDASKISNELNWQPQETFETGLCKTVEWYLANQEWCQHVQDGTYQRERLGI
CCCCCEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure
MKILVTGGAGFIGSAVVRHIISNTQDSVINLDKLTYAGNLESLVSVEASERYAFEQVDIC
CEEEEECCCCHHHHHHHHHHHCCCCCCEEEHHHHEECCCHHHHHHHHHHHHHHHHHCCCH
DRAELDRVFSQYKPDAVMHLAAESHVDRSITGPADFIQTNIVGTYTLLEAARHYWMQLDA
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
ERKVAFRFHHISTDEVYGDLPHPDEQEGQVVNQELPLFTETTPYAPSSPYSASKASSDHL
CCEEEEEEEECCHHHHCCCCCCCCCCCCCEECCCCCCEECCCCCCCCCCCCCCCCCCHHH
VRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKQLPIYGKGDQIRDWLYVE
HHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCEEEEH
DHARALYKVVTEGQIGETYNIGGHNEKQNLEVVQTICTILDALVPKASSYAEQITYVTDR
HHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEECC
PGHDRRYAIDASKISNELNWQPQETFETGLCKTVEWYLANQEWCQHVQDGTYQRERLGI
CCCCCEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609; 11796113 [H]