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The map label for this gene is fliA [H]

Identifier: 113969642

GI number: 113969642

Start: 1506549

End: 1507262

Strand: Direct

Name: fliA [H]

Synonym: Shewmr4_1299

Alternate gene names: 113969642

Gene position: 1506549-1507262 (Clockwise)

Preceding gene: 113969641

Following gene: 113969643

Centisome position: 32.01

GC content: 44.4

Gene sequence:

>714_bases
GTGAATAAAGCCGCAGCGTATACTTGTTTAGACAGTAAAACGTCAATCGTTGAACAGTATGCACCGTTGGTTAAAAGAAT
TGCACATCACTTGCTTGCCAGATTACCTGCCTCAGTGCAGCTCGATGACTTGCTTCAAGCGGGGATGATGGGGTTATTAG
AAGCATCATCCAAGTTTGATGGTGGCAAAGGTGCAAAGTTTGAAACCTTCGCCGGCATAAGGATCCGTGGCGCCATGTTG
GATGAGATCCGTCGTGGCGATTGGGTTCCCCGTTCTGTTCATCGTAATAATCGTCGAGTTGCTCAAGCCATTGATGAGTT
AGAGCAAGTGTTGGGTCGTGATGCTCGCGACACAGAAATTGCAGAAAAACTTGATATGACGCTCGATGAGTACCATCATA
TTCTTAACGATGTTTCTGTTGGTAAAATCATAGGGATAGAAGACTTAGGCGTATCACAGGATATACTCACTCCGAGCGAT
AATGTGAGTGATGGTACATTTGAAGCACTAGCAGAGACCCAGTTTCATTCAGCATTAGTAGAGGCGATAAAATTATTGCC
CGAAAGAGATGCGCTAGTGCTATCGTTATACTACGATGAAGCATTGAATTTAAAAGAAATTGGCGCCATTCTTGAGGTGA
GCGAGTCGCGAGTTAGCCAGATATTAAGTCAGGCAATGTTGCGACTCAAAGGCAAACTCAAGCATTGGACATAA

Upstream 100 bases:

>100_bases
AAGATCATGAGTTGGCCAGTACCACAGCAACCTGGTGGGCATTTGGAGTTTTTCGTTGAGCGTTTAGTTCAGCGACCAGA
TTTTCAAGAGGAAAAAACGA

Downstream 100 bases:

>100_bases
GTATAATTATTTATTGAGCACAGAATGTCAGCTCACCGGAGGAAACCTTGGACAAGAATATGAAGATTCTCATCGTTGAT
GACTTTTCGACAATGAGACG

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MNKAAAYTCLDSKTSIVEQYAPLVKRIAHHLLARLPASVQLDDLLQAGMMGLLEASSKFDGGKGAKFETFAGIRIRGAML
DEIRRGDWVPRSVHRNNRRVAQAIDELEQVLGRDARDTEIAEKLDMTLDEYHHILNDVSVGKIIGIEDLGVSQDILTPSD
NVSDGTFEALAETQFHSALVEAIKLLPERDALVLSLYYDEALNLKEIGAILEVSESRVSQILSQAMLRLKGKLKHWT

Sequences:

>Translated_237_residues
MNKAAAYTCLDSKTSIVEQYAPLVKRIAHHLLARLPASVQLDDLLQAGMMGLLEASSKFDGGKGAKFETFAGIRIRGAML
DEIRRGDWVPRSVHRNNRRVAQAIDELEQVLGRDARDTEIAEKLDMTLDEYHHILNDVSVGKIIGIEDLGVSQDILTPSD
NVSDGTFEALAETQFHSALVEAIKLLPERDALVLSLYYDEALNLKEIGAILEVSESRVSQILSQAMLRLKGKLKHWT
>Mature_237_residues
MNKAAAYTCLDSKTSIVEQYAPLVKRIAHHLLARLPASVQLDDLLQAGMMGLLEASSKFDGGKGAKFETFAGIRIRGAML
DEIRRGDWVPRSVHRNNRRVAQAIDELEQVLGRDARDTEIAEKLDMTLDEYHHILNDVSVGKIIGIEDLGVSQDILTPSD
NVSDGTFEALAETQFHSALVEAIKLLPERDALVLSLYYDEALNLKEIGAILEVSESRVSQILSQAMLRLKGKLKHWT

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=237, Percent_Identity=49.3670886075949, Blast_Score=211, Evalue=2e-56,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 26304; Mature: 26304

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKAAAYTCLDSKTSIVEQYAPLVKRIAHHLLARLPASVQLDDLLQAGMMGLLEASSKFD
CCCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
GGKGAKFETFAGIRIRGAMLDEIRRGDWVPRSVHRNNRRVAQAIDELEQVLGRDARDTEI
CCCCCCEEHCCCCEEHHHHHHHHHCCCCCCHHHHHCHHHHHHHHHHHHHHHCCCCCHHHH
AEKLDMTLDEYHHILNDVSVGKIIGIEDLGVSQDILTPSDNVSDGTFEALAETQFHSALV
HHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EAIKLLPERDALVLSLYYDEALNLKEIGAILEVSESRVSQILSQAMLRLKGKLKHWT
HHHHHCCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNKAAAYTCLDSKTSIVEQYAPLVKRIAHHLLARLPASVQLDDLLQAGMMGLLEASSKFD
CCCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
GGKGAKFETFAGIRIRGAMLDEIRRGDWVPRSVHRNNRRVAQAIDELEQVLGRDARDTEI
CCCCCCEEHCCCCEEHHHHHHHHHCCCCCCHHHHHCHHHHHHHHHHHHHHHCCCCCHHHH
AEKLDMTLDEYHHILNDVSVGKIIGIEDLGVSQDILTPSDNVSDGTFEALAETQFHSALV
HHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EAIKLLPERDALVLSLYYDEALNLKEIGAILEVSESRVSQILSQAMLRLKGKLKHWT
HHHHHCCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1560774; 10984043 [H]