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The map label for this gene is hisS [H]

Identifier: 113969573

GI number: 113969573

Start: 1429150

End: 1430427

Strand: Direct

Name: hisS [H]

Synonym: Shewmr4_1229

Alternate gene names: 113969573

Gene position: 1429150-1430427 (Clockwise)

Preceding gene: 113969572

Following gene: 113969574

Centisome position: 30.37

GC content: 49.3

Gene sequence:

>1278_bases
GTGGCAAAACAGATCCAAGCGATTCGCGGAATGAACGACATTCTGCCAACTCAAAGTCCTCTATGGCAAAAAGTGGAAGC
GGTACTACGTGCGAGCGTGGCTGCTTATGGTTACAGTGAGATCCGCACTCCAATAGTGGAAAATACCGATCTTTTTAAAC
GCTCTATCGGTGAAGTGACTGATATTGTTGAAAAAGAAATGTATACCTTCGAGGACCGTAACGGTGACAGTTTAACCCTA
CGTCCTGAAGGCACCGCCTCAACCGTGCGTGCGGGTAATGAGCATGGTCTGCTCTATAACCAAGAACAACGCCTGTGGTA
CATGGGCCCAATGTTCCGCCACGAACGTCCGCAAAAAGGTCGTTATCGCCAATTCCACCAGTTTGGTGTGGAAGTCTACG
GCATTGGTAGCGCCGATATCGACGCCGAAGTGTTGATGTTATCGGCCCGTCTATGGGAAAAACTCGGCATTAGCGAGCAT
GTGACGCTTGAGCTGAACACCTTAGGTGACCCTGCTGAGCGCGCCGCTTACCGTGAAGCCTTGATTGCCTTCTTAGAGCA
ACACAAAGACAAATTAGATGAAGATAGCCAGCGCCGTATGTATAGCAATCCGCTGCGGGTATTGGACTCAAAAGATCCCC
AAGTGCAAAGCATTTTAGGTGATGCGCCTGCGCTGATGGATTACTTAGGTGAAGAATCTTCACAACATTTTGCACAATTG
CGTGAACTCCTCGACGCGGTTGGCATCCAATACCGAGTTAACCCCCGCTTAGTCCGTGGTTTAGATTACTACAATCGCAC
GGTTTTTGAATGGGTTACCAATAGCTTAGGCTCTCAAGGTACTGTCCTTGCGGGCGGTCGTTACGATGGTTTAGTCGCTC
AATTGGGCGGTAAAGATACGCCAGCCGTCGGTTTTGCGATGGGATTAGAGCGCATCGTTCTATTGCTTGAAACCTTAGAA
CTGACCCAAGATATCCCTGCGGCTGTCGATGTGTATGTCGCAGCGATGGGGGATAGCTGTTTGGTTGAAGCCATAAAAGT
GGCACAGGAGTTACGCTCAACCTTACCGACACTGCGTGTCATGAGCCACTGCGGCGGCGGTAACTTCAAGAAGCAAATTA
AGCGTGCCGATAAAAGCGGCGCACAAGTGGCCTTACTCATCGGTGAAGAAGAACTCGCCGAAGGTGTGGTTACCGTTAAA
TATTTACGCAATGACAACGAACAACAACGGGTCGCTCGAAATGCACTAAGCGCATTTTTAGCTGAACTTACCAAATAA

Upstream 100 bases:

>100_bases
AGACCCCGAAACCGACGGGGTCATTTTACGTTTAGTGTTGAACAGCCTATAATGCCTGACATTTTTTATTAATTTAGCGA
AAATCGGACGAGTCGATACA

Downstream 100 bases:

>100_bases
GGGGACGTGCGCGTGGAAATTTATAGCACAGAAGAACAACAAGTTGATGCTATCAAGCAGTTCTGGAAAGACTATGGAAC
CTCGATTGTTATCGGGGCGG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MAKQIQAIRGMNDILPTQSPLWQKVEAVLRASVAAYGYSEIRTPIVENTDLFKRSIGEVTDIVEKEMYTFEDRNGDSLTL
RPEGTASTVRAGNEHGLLYNQEQRLWYMGPMFRHERPQKGRYRQFHQFGVEVYGIGSADIDAEVLMLSARLWEKLGISEH
VTLELNTLGDPAERAAYREALIAFLEQHKDKLDEDSQRRMYSNPLRVLDSKDPQVQSILGDAPALMDYLGEESSQHFAQL
RELLDAVGIQYRVNPRLVRGLDYYNRTVFEWVTNSLGSQGTVLAGGRYDGLVAQLGGKDTPAVGFAMGLERIVLLLETLE
LTQDIPAAVDVYVAAMGDSCLVEAIKVAQELRSTLPTLRVMSHCGGGNFKKQIKRADKSGAQVALLIGEEELAEGVVTVK
YLRNDNEQQRVARNALSAFLAELTK

Sequences:

>Translated_425_residues
MAKQIQAIRGMNDILPTQSPLWQKVEAVLRASVAAYGYSEIRTPIVENTDLFKRSIGEVTDIVEKEMYTFEDRNGDSLTL
RPEGTASTVRAGNEHGLLYNQEQRLWYMGPMFRHERPQKGRYRQFHQFGVEVYGIGSADIDAEVLMLSARLWEKLGISEH
VTLELNTLGDPAERAAYREALIAFLEQHKDKLDEDSQRRMYSNPLRVLDSKDPQVQSILGDAPALMDYLGEESSQHFAQL
RELLDAVGIQYRVNPRLVRGLDYYNRTVFEWVTNSLGSQGTVLAGGRYDGLVAQLGGKDTPAVGFAMGLERIVLLLETLE
LTQDIPAAVDVYVAAMGDSCLVEAIKVAQELRSTLPTLRVMSHCGGGNFKKQIKRADKSGAQVALLIGEEELAEGVVTVK
YLRNDNEQQRVARNALSAFLAELTK
>Mature_424_residues
AKQIQAIRGMNDILPTQSPLWQKVEAVLRASVAAYGYSEIRTPIVENTDLFKRSIGEVTDIVEKEMYTFEDRNGDSLTLR
PEGTASTVRAGNEHGLLYNQEQRLWYMGPMFRHERPQKGRYRQFHQFGVEVYGIGSADIDAEVLMLSARLWEKLGISEHV
TLELNTLGDPAERAAYREALIAFLEQHKDKLDEDSQRRMYSNPLRVLDSKDPQVQSILGDAPALMDYLGEESSQHFAQLR
ELLDAVGIQYRVNPRLVRGLDYYNRTVFEWVTNSLGSQGTVLAGGRYDGLVAQLGGKDTPAVGFAMGLERIVLLLETLEL
TQDIPAAVDVYVAAMGDSCLVEAIKVAQELRSTLPTLRVMSHCGGGNFKKQIKRADKSGAQVALLIGEEELAEGVVTVKY
LRNDNEQQRVARNALSAFLAELTK

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI15029520, Length=461, Percent_Identity=24.7288503253796, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI6996014, Length=442, Percent_Identity=25.7918552036199, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1788861, Length=423, Percent_Identity=67.1394799054374, Blast_Score=589, Evalue=1e-169,
Organism=Caenorhabditis elegans, GI71993693, Length=459, Percent_Identity=26.797385620915, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71993686, Length=459, Percent_Identity=26.797385620915, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6325290, Length=455, Percent_Identity=24.3956043956044, Blast_Score=89, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 47482; Mature: 47350

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQIQAIRGMNDILPTQSPLWQKVEAVLRASVAAYGYSEIRTPIVENTDLFKRSIGEVT
CCCHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHH
DIVEKEMYTFEDRNGDSLTLRPEGTASTVRAGNEHGLLYNQEQRLWYMGPMFRHERPQKG
HHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCCCCCCEECCCCCEEEECCHHHCCCCCCC
RYRQFHQFGVEVYGIGSADIDAEVLMLSARLWEKLGISEHVTLELNTLGDPAERAAYREA
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHH
LIAFLEQHKDKLDEDSQRRMYSNPLRVLDSKDPQVQSILGDAPALMDYLGEESSQHFAQL
HHHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCCHHHHHHCCHHHHHHHHCCCHHHHHHHH
RELLDAVGIQYRVNPRLVRGLDYYNRTVFEWVTNSLGSQGTVLAGGRYDGLVAQLGGKDT
HHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHCCCCCC
PAVGFAMGLERIVLLLETLELTQDIPAAVDVYVAAMGDSCLVEAIKVAQELRSTLPTLRV
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
MSHCGGGNFKKQIKRADKSGAQVALLIGEEELAEGVVTVKYLRNDNEQQRVARNALSAFL
HHHCCCCCHHHHHHHHCCCCCEEEEEECHHHHHCHHEEEHHHHCCCHHHHHHHHHHHHHH
AELTK
HHHCC
>Mature Secondary Structure 
AKQIQAIRGMNDILPTQSPLWQKVEAVLRASVAAYGYSEIRTPIVENTDLFKRSIGEVT
CCHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHH
DIVEKEMYTFEDRNGDSLTLRPEGTASTVRAGNEHGLLYNQEQRLWYMGPMFRHERPQKG
HHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCCCCCCEECCCCCEEEECCHHHCCCCCCC
RYRQFHQFGVEVYGIGSADIDAEVLMLSARLWEKLGISEHVTLELNTLGDPAERAAYREA
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHH
LIAFLEQHKDKLDEDSQRRMYSNPLRVLDSKDPQVQSILGDAPALMDYLGEESSQHFAQL
HHHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCCHHHHHHCCHHHHHHHHCCCHHHHHHHH
RELLDAVGIQYRVNPRLVRGLDYYNRTVFEWVTNSLGSQGTVLAGGRYDGLVAQLGGKDT
HHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHCCCCCC
PAVGFAMGLERIVLLLETLELTQDIPAAVDVYVAAMGDSCLVEAIKVAQELRSTLPTLRV
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
MSHCGGGNFKKQIKRADKSGAQVALLIGEEELAEGVVTVKYLRNDNEQQRVARNALSAFL
HHHCCCCCHHHHHHHHCCCCCEEEEEECHHHHHCHHEEEHHHHCCCHHHHHHHHHHHHHH
AELTK
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]