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The map label for this gene is ubiF [H]

Identifier: 113969350

GI number: 113969350

Start: 1173177

End: 1174403

Strand: Direct

Name: ubiF [H]

Synonym: Shewmr4_1006

Alternate gene names: 113969350

Gene position: 1173177-1174403 (Clockwise)

Preceding gene: 113969342

Following gene: 113969351

Centisome position: 24.93

GC content: 51.26

Gene sequence:

>1227_bases
ATGAGTCAAGCGATAAGCCAAACACTGCAGCCTGAACAGACAACCTCAGACACTCGCGTATTTGATGTGATTTTAGTTGG
TGGCGGCATGGTGGGGGCGGCGACGGCCATAGGGCTGGCAAAGCAAGGATTACAGGTGGCCGTGATTGAATCATTTGCCC
CCGAGGCTTATTCCCCTGAGCAGGCATTAGATGTGCGTGTGTCGGCGATAAGCGTGGCCTCTGAACAGTTGCTCGGGCAG
TTAGGCGCGCTCGAGAGCCTGCTTAACATGCGTAATGTGCCTTATCTGGGCTTAGAAACCTGGGAGCTTGATGGCTGCAT
TACTCAATTCCATAGCTCACAAATCGGCGCAAGCCATTTAGGGCATATTGTTGAAAACCGTTTGGTGCAACTTGCCCTGT
GGGAGCAAATGCAGCAATGGGATGCGATTAAATTGTTTTGCCCCGAACGTGTGGCGACATTTTCGCGCCTCACAGACGGG
GTGAGTGTGCACTTACAATCCGGTATTCAACTTGAGGCAAAGCTGCTCATCGGAGCTGATGGCGCCAACTCGCAGGTGCG
CCAATGGGCGGGGATTGGCATCTCTGGGTGGGACTATGCCCAATCGGCGATGCTGATTAATATCCAAACCGCCCAAGGTC
AGCAAGATGTCACTTGGCAACAATTTACCCCTAACGGCCCGCGCTCGTTATTACCTCTCCCCGGCAATCATGCGTCGTTA
GTCTGGTATGACGATGCCAATCGCATTAAACAGTTAATGCAGCTGAATCATAAGCAGCTTGCCGACCAAATTCGCCAACA
TTTTCCCGCTCGCTTAGACAGCGACTTTACCGTTGAAGCCAAAGGCAGTTTTGCCCTCACGCGCCGCCACGCGAATGCCT
ATTTTAAGTCGAATGTGGTGATTTTAGGGGATGCGGCGCACACAATTAACCCGTTGGCTGGCCAAGGGGTTAACCTCGGC
TTTAAAGATGTCGAGGCCTTACTAACGGTAATCAAGGCTGCATTAACCGAGGATAAACCTTGGTGGTCCACCGAGGTATT
AAACGCCTATCAAGCCAAGCGTTATCGCGATAATCAGCTGATGATGACGACCATGGATGTGTTTTACGCAGGCTTTAGTA
ACGACATTCTGCCACTTAAATTACTGCGTAATGGCGCGTTAAAACTGGCCAATATTAACTCCCCCATCAAGCGAACCGTG
CTGAAGTATGCGATGGGCCTCAATTAG

Upstream 100 bases:

>100_bases
GATTAAGAGTGATGTGCTTTAACTGAGATAAAGTGATGTGCTTTAACTGAGATAAAGTGCGTCAGATAAATGGAAAGAAC
AGTAAAGAGTGGTAACTAAG

Downstream 100 bases:

>100_bases
GCCACATCGAGAGTGCTGACTCACTCGGCATTTGTTGACTTACGCATTGAGTGCTAAAATGCCGCGTTTTTAACGAGCAT
AAGCGGCATTATTAACTCAA

Product: 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MSQAISQTLQPEQTTSDTRVFDVILVGGGMVGAATAIGLAKQGLQVAVIESFAPEAYSPEQALDVRVSAISVASEQLLGQ
LGALESLLNMRNVPYLGLETWELDGCITQFHSSQIGASHLGHIVENRLVQLALWEQMQQWDAIKLFCPERVATFSRLTDG
VSVHLQSGIQLEAKLLIGADGANSQVRQWAGIGISGWDYAQSAMLINIQTAQGQQDVTWQQFTPNGPRSLLPLPGNHASL
VWYDDANRIKQLMQLNHKQLADQIRQHFPARLDSDFTVEAKGSFALTRRHANAYFKSNVVILGDAAHTINPLAGQGVNLG
FKDVEALLTVIKAALTEDKPWWSTEVLNAYQAKRYRDNQLMMTTMDVFYAGFSNDILPLKLLRNGALKLANINSPIKRTV
LKYAMGLN

Sequences:

>Translated_408_residues
MSQAISQTLQPEQTTSDTRVFDVILVGGGMVGAATAIGLAKQGLQVAVIESFAPEAYSPEQALDVRVSAISVASEQLLGQ
LGALESLLNMRNVPYLGLETWELDGCITQFHSSQIGASHLGHIVENRLVQLALWEQMQQWDAIKLFCPERVATFSRLTDG
VSVHLQSGIQLEAKLLIGADGANSQVRQWAGIGISGWDYAQSAMLINIQTAQGQQDVTWQQFTPNGPRSLLPLPGNHASL
VWYDDANRIKQLMQLNHKQLADQIRQHFPARLDSDFTVEAKGSFALTRRHANAYFKSNVVILGDAAHTINPLAGQGVNLG
FKDVEALLTVIKAALTEDKPWWSTEVLNAYQAKRYRDNQLMMTTMDVFYAGFSNDILPLKLLRNGALKLANINSPIKRTV
LKYAMGLN
>Mature_407_residues
SQAISQTLQPEQTTSDTRVFDVILVGGGMVGAATAIGLAKQGLQVAVIESFAPEAYSPEQALDVRVSAISVASEQLLGQL
GALESLLNMRNVPYLGLETWELDGCITQFHSSQIGASHLGHIVENRLVQLALWEQMQQWDAIKLFCPERVATFSRLTDGV
SVHLQSGIQLEAKLLIGADGANSQVRQWAGIGISGWDYAQSAMLINIQTAQGQQDVTWQQFTPNGPRSLLPLPGNHASLV
WYDDANRIKQLMQLNHKQLADQIRQHFPARLDSDFTVEAKGSFALTRRHANAYFKSNVVILGDAAHTINPLAGQGVNLGF
KDVEALLTVIKAALTEDKPWWSTEVLNAYQAKRYRDNQLMMTTMDVFYAGFSNDILPLKLLRNGALKLANINSPIKRTVL
KYAMGLN

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=388, Percent_Identity=31.4432989690722, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI32967307, Length=331, Percent_Identity=31.7220543806647, Blast_Score=152, Evalue=5e-37,
Organism=Escherichia coli, GI1786883, Length=386, Percent_Identity=48.1865284974093, Blast_Score=355, Evalue=3e-99,
Organism=Escherichia coli, GI1789273, Length=390, Percent_Identity=37.6923076923077, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI1789274, Length=402, Percent_Identity=27.363184079602, Blast_Score=161, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI25146631, Length=434, Percent_Identity=27.6497695852535, Blast_Score=155, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6321694, Length=456, Percent_Identity=24.780701754386, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19920760, Length=438, Percent_Identity=29.4520547945205, Blast_Score=149, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 44865; Mature: 44734

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQAISQTLQPEQTTSDTRVFDVILVGGGMVGAATAIGLAKQGLQVAVIESFAPEAYSPE
CCHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCC
QALDVRVSAISVASEQLLGQLGALESLLNMRNVPYLGLETWELDGCITQFHSSQIGASHL
HHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCEEEEHHHHHHHHHHCCCCHHHH
GHIVENRLVQLALWEQMQQWDAIKLFCPERVATFSRLTDGVSVHLQSGIQLEAKLLIGAD
HHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEECCCCEEEEEEEEECC
GANSQVRQWAGIGISGWDYAQSAMLINIQTAQGQQDVTWQQFTPNGPRSLLPLPGNHASL
CCCHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCEECCCCCCCCCCCCCCCCEEE
VWYDDANRIKQLMQLNHKQLADQIRQHFPARLDSDFTVEAKGSFALTRRHANAYFKSNVV
EEECCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEHHHHHHHHCCEE
ILGDAAHTINPLAGQGVNLGFKDVEALLTVIKAALTEDKPWWSTEVLNAYQAKRYRDNQL
EEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEE
MMTTMDVFYAGFSNDILPLKLLRNGALKLANINSPIKRTVLKYAMGLN
EEEEHHHHHHCCCCCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQAISQTLQPEQTTSDTRVFDVILVGGGMVGAATAIGLAKQGLQVAVIESFAPEAYSPE
CHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCC
QALDVRVSAISVASEQLLGQLGALESLLNMRNVPYLGLETWELDGCITQFHSSQIGASHL
HHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCEEEEHHHHHHHHHHCCCCHHHH
GHIVENRLVQLALWEQMQQWDAIKLFCPERVATFSRLTDGVSVHLQSGIQLEAKLLIGAD
HHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEECCCCEEEEEEEEECC
GANSQVRQWAGIGISGWDYAQSAMLINIQTAQGQQDVTWQQFTPNGPRSLLPLPGNHASL
CCCHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCEECCCCCCCCCCCCCCCCEEE
VWYDDANRIKQLMQLNHKQLADQIRQHFPARLDSDFTVEAKGSFALTRRHANAYFKSNVV
EEECCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEHHHHHHHHCCEE
ILGDAAHTINPLAGQGVNLGFKDVEALLTVIKAALTEDKPWWSTEVLNAYQAKRYRDNQL
EEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEE
MMTTMDVFYAGFSNDILPLKLLRNGALKLANINSPIKRTVLKYAMGLN
EEEEHHHHHHCCCCCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]