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The map label for this gene is rlmI [H]

Identifier: 113969317

GI number: 113969317

Start: 1139869

End: 1141059

Strand: Direct

Name: rlmI [H]

Synonym: Shewmr4_0973

Alternate gene names: 113969317

Gene position: 1139869-1141059 (Clockwise)

Preceding gene: 113969313

Following gene: 113969319

Centisome position: 24.22

GC content: 51.55

Gene sequence:

>1191_bases
ATGGCAGTCAGAATCAAACTTAAACCCGGTCGCGAAAAGTCCCTTGAGCGTCGTCATCCTTGGGTTTTTTCCAACGGGAT
CCACAACGTTAAAGGTAAACTCGAAGCGGGCGATACCGTCGATGTGGTTGCCCATGATGGTCATTGGTTGGGCCGTGGTG
CTTGGTCTCCTGAGTCTCAAATCCAAGTGCGGATTTGGACTTTCGATCGCGAAGAAGAAATTGACCGTGAGTTTTTTAAG
CGCCGTATTCTGCGCGCCCAAGCGGGACGCGACGATCTGATTCGTGAGCAAGGCTTAACCGGTTACCGCTTAATTGCCGC
CGAGTCCGATGGTCTGCCCGGTATCACCATTGATAAATACGCTAATGTGTTGGTCTGCCAATTGCTGAGCATGGGCGCCG
ATGTATGGCGTGACACTATTGTCGATGTGCTCGCCGAGCTGTATCCCGATTGCGCGATTTACGAGCGCTCAGATGTCGAT
TCCCGTAAGAAAGAAGGCTTAGCCTCTACCATGGGACTGCTCCATGGCACGCTGCCTGAAATGCCCGTCATTATTGAAGA
AAACGGTATCAAGATTGCCGTCGATGTGACTAAGGGTCATAAGACTGGTTTCTACCTCGACCAGCGCGACAACCGTGCCA
TGGCCGCGCGGTTTGTGAAGGGCAAATCGGTGCTGAACTGTTTTTGCTACACGGGCACCTTTGGCCTGTATGCCGCTAAG
GCGGGCGCCGCCAGCATTGAAAACGTCGACGTGTCGGCACTTGCCCTCGATACCGCGCGTTTGAACATGCGAGTCAATGG
CTTAAATGATGATAATGTGCATTACAACGAAGCCGATGTGTTTAAGCTGTTACGTCAGTACCGCGATGAGGGTAAAACCT
TCGATGTGATCGTGCTCGACCCGCCAAAGTTTGCCGACAATAAGTCGCAGCTCAATGGCGCCTGTCGTGGCTATAAAGAT
ATCAATATGATTGCGCTACAATTATTAAATCCGGGCGGGGTGCTGTTGACCTTCTCTTGCTCGGGATTGATGCCTGCGGA
TCTGTTCCAAAAAATCGTTGCCGATGCGGCACTGGATGCTAAGCGTGAGATCCAATTTATCGAGCGTTTAAGTCAGGCGA
GTGATCACCCGATTGGTAGTGCCTTCCCTGAAGGTTTTTACCTCAAGGGCTTAGTAGCTCGAGTGTGGTAA

Upstream 100 bases:

>100_bases
ATTATCCGCTTCCATTTTTTGAAGACGGCGCCTGACGAACTTAACGCAGTTTGTTGTGGCGGTTCAATTTAGCAAGGCGG
TACTTTTTTAAGGCGGTACT

Downstream 100 bases:

>100_bases
TCGCTTAATGGCCTTAGTGTTATAAAAAATGAGGGTCATAAAAAATGCCGGTGCAGTTGCACTGGCATTTTTGTTTCTCG
CATGGGAGTCTTTCGACTCA

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAVRIKLKPGREKSLERRHPWVFSNGIHNVKGKLEAGDTVDVVAHDGHWLGRGAWSPESQIQVRIWTFDREEEIDREFFK
RRILRAQAGRDDLIREQGLTGYRLIAAESDGLPGITIDKYANVLVCQLLSMGADVWRDTIVDVLAELYPDCAIYERSDVD
SRKKEGLASTMGLLHGTLPEMPVIIEENGIKIAVDVTKGHKTGFYLDQRDNRAMAARFVKGKSVLNCFCYTGTFGLYAAK
AGAASIENVDVSALALDTARLNMRVNGLNDDNVHYNEADVFKLLRQYRDEGKTFDVIVLDPPKFADNKSQLNGACRGYKD
INMIALQLLNPGGVLLTFSCSGLMPADLFQKIVADAALDAKREIQFIERLSQASDHPIGSAFPEGFYLKGLVARVW

Sequences:

>Translated_396_residues
MAVRIKLKPGREKSLERRHPWVFSNGIHNVKGKLEAGDTVDVVAHDGHWLGRGAWSPESQIQVRIWTFDREEEIDREFFK
RRILRAQAGRDDLIREQGLTGYRLIAAESDGLPGITIDKYANVLVCQLLSMGADVWRDTIVDVLAELYPDCAIYERSDVD
SRKKEGLASTMGLLHGTLPEMPVIIEENGIKIAVDVTKGHKTGFYLDQRDNRAMAARFVKGKSVLNCFCYTGTFGLYAAK
AGAASIENVDVSALALDTARLNMRVNGLNDDNVHYNEADVFKLLRQYRDEGKTFDVIVLDPPKFADNKSQLNGACRGYKD
INMIALQLLNPGGVLLTFSCSGLMPADLFQKIVADAALDAKREIQFIERLSQASDHPIGSAFPEGFYLKGLVARVW
>Mature_395_residues
AVRIKLKPGREKSLERRHPWVFSNGIHNVKGKLEAGDTVDVVAHDGHWLGRGAWSPESQIQVRIWTFDREEEIDREFFKR
RILRAQAGRDDLIREQGLTGYRLIAAESDGLPGITIDKYANVLVCQLLSMGADVWRDTIVDVLAELYPDCAIYERSDVDS
RKKEGLASTMGLLHGTLPEMPVIIEENGIKIAVDVTKGHKTGFYLDQRDNRAMAARFVKGKSVLNCFCYTGTFGLYAAKA
GAASIENVDVSALALDTARLNMRVNGLNDDNVHYNEADVFKLLRQYRDEGKTFDVIVLDPPKFADNKSQLNGACRGYKDI
NMIALQLLNPGGVLLTFSCSGLMPADLFQKIVADAALDAKREIQFIERLSQASDHPIGSAFPEGFYLKGLVARVW

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=395, Percent_Identity=58.4810126582278, Blast_Score=487, Evalue=1e-139,
Organism=Escherichia coli, GI1787181, Length=248, Percent_Identity=28.6290322580645, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 44134; Mature: 44003

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVRIKLKPGREKSLERRHPWVFSNGIHNVKGKLEAGDTVDVVAHDGHWLGRGAWSPESQ
CEEEEEECCCCCHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEEECCEEECCCCCCCCCC
IQVRIWTFDREEEIDREFFKRRILRAQAGRDDLIREQGLTGYRLIAAESDGLPGITIDKY
EEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEECCCCCCCCCHHHH
ANVLVCQLLSMGADVWRDTIVDVLAELYPDCAIYERSDVDSRKKEGLASTMGLLHGTLPE
HHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCC
MPVIIEENGIKIAVDVTKGHKTGFYLDQRDNRAMAARFVKGKSVLNCFCYTGTFGLYAAK
CCEEEECCCEEEEEEEECCCCCCEEEECCCCHHHHHHHHCCCHHEEEEEECCCCHHHHHC
AGAASIENVDVSALALDTARLNMRVNGLNDDNVHYNEADVFKLLRQYRDEGKTFDVIVLD
CCCCCCCCCCEEEEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEC
PPKFADNKSQLNGACRGYKDINMIALQLLNPGGVLLTFSCSGLMPADLFQKIVADAALDA
CCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCH
KREIQFIERLSQASDHPIGSAFPEGFYLKGLVARVW
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AVRIKLKPGREKSLERRHPWVFSNGIHNVKGKLEAGDTVDVVAHDGHWLGRGAWSPESQ
EEEEEECCCCCHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEEECCEEECCCCCCCCCC
IQVRIWTFDREEEIDREFFKRRILRAQAGRDDLIREQGLTGYRLIAAESDGLPGITIDKY
EEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEECCCCCCCCCHHHH
ANVLVCQLLSMGADVWRDTIVDVLAELYPDCAIYERSDVDSRKKEGLASTMGLLHGTLPE
HHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCC
MPVIIEENGIKIAVDVTKGHKTGFYLDQRDNRAMAARFVKGKSVLNCFCYTGTFGLYAAK
CCEEEECCCEEEEEEEECCCCCCEEEECCCCHHHHHHHHCCCHHEEEEEECCCCHHHHHC
AGAASIENVDVSALALDTARLNMRVNGLNDDNVHYNEADVFKLLRQYRDEGKTFDVIVLD
CCCCCCCCCCEEEEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEC
PPKFADNKSQLNGACRGYKDINMIALQLLNPGGVLLTFSCSGLMPADLFQKIVADAALDA
CCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCH
KREIQFIERLSQASDHPIGSAFPEGFYLKGLVARVW
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA