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The map label for this gene is cobS [H]

Identifier: 113969187

GI number: 113969187

Start: 998408

End: 999196

Strand: Direct

Name: cobS [H]

Synonym: Shewmr4_0843

Alternate gene names: 113969187

Gene position: 998408-999196 (Clockwise)

Preceding gene: 113969186

Following gene: 113969188

Centisome position: 21.21

GC content: 52.47

Gene sequence:

>789_bases
ATGTCAGAACGCGAAAGCTGGCACAAAGAGATAGATTTGTTTTTAGTGGCCATGGGCTATTTTACCCGCATTCCTATGCC
CAAATGGGTTGAGGTCGATGCCGACAAACTCAATAAAGCCAGCCGTTACTTTGGGTTAGTGGGTCTGCTGGTGGGGCTAC
TCAGTGCCATCGTGTTTTGGCTTACCCAAAACTGGTTGCCCGCCGGCGTGTCTGTACTGCTGTCGATGGTCACAGGCGTG
CTGCTCACGGGCGGTTTTCACGAGGACGGTTTAGCCGATACCTTCGATGGTTTCGGTGGCGGTTGGACGGCGGAAGACAA
GCTGCGGATCATGAAAGACTCTCGCCTAGGCAGCTACGGCGCCTTGGCTTTGATGCTCGTGCTGATGCTCAAGTGGCAAT
TATTAGTGGAACTCGCGCTTTATGATCCTGTGGTTGCAGGCTCGGCGATGATAGTCGCCCACACTGTGAGCCGTGTCGTT
GCCGCCAGCCTTATCTTTACCGAGAAATACGTGCGCGACGATGAATCCAGTAAATCCAAGCCGTTAGCGCAGCATCAAGG
TATTAATGAACTGTTTATTTTGATCGCCAGCGGTGTGTTAGTGCTGTTGGTGCTTAAGGGCATTGCCGCGTTGTCGTTGT
TACTGGTGATGATTGGCCTACGCCGTTTGATTGTGGTGATCTTTAGACGCCAAATCGGTGGCTACACGGGTGATACCTTA
GGCGCGGCGCAACAGATCTGCGAAATCGTCTGTTACTTCGTGCTGCTCGTTGTGGGTAGCATACTGTGA

Upstream 100 bases:

>100_bases
ATGATGTGCCCTGCTAAGTGTCTTATTTAAAATAATAACTTTTAGCTAACCGTTTTAATCTTAAACCTTGCAGCAAGTGT
TAATTCACTAAGGATGCTTT

Downstream 100 bases:

>100_bases
TCCATTTAGTGCTAGGCGGCGCGCGTAGCGGTAAGAGCCGCTACGGCGAAGCCTTAGTGCGCCAGTATACCGCCCTCGGA
TTTGATGCCTGCTATCTGGC

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MSERESWHKEIDLFLVAMGYFTRIPMPKWVEVDADKLNKASRYFGLVGLLVGLLSAIVFWLTQNWLPAGVSVLLSMVTGV
LLTGGFHEDGLADTFDGFGGGWTAEDKLRIMKDSRLGSYGALALMLVLMLKWQLLVELALYDPVVAGSAMIVAHTVSRVV
AASLIFTEKYVRDDESSKSKPLAQHQGINELFILIASGVLVLLVLKGIAALSLLLVMIGLRRLIVVIFRRQIGGYTGDTL
GAAQQICEIVCYFVLLVVGSIL

Sequences:

>Translated_262_residues
MSERESWHKEIDLFLVAMGYFTRIPMPKWVEVDADKLNKASRYFGLVGLLVGLLSAIVFWLTQNWLPAGVSVLLSMVTGV
LLTGGFHEDGLADTFDGFGGGWTAEDKLRIMKDSRLGSYGALALMLVLMLKWQLLVELALYDPVVAGSAMIVAHTVSRVV
AASLIFTEKYVRDDESSKSKPLAQHQGINELFILIASGVLVLLVLKGIAALSLLLVMIGLRRLIVVIFRRQIGGYTGDTL
GAAQQICEIVCYFVLLVVGSIL
>Mature_261_residues
SERESWHKEIDLFLVAMGYFTRIPMPKWVEVDADKLNKASRYFGLVGLLVGLLSAIVFWLTQNWLPAGVSVLLSMVTGVL
LTGGFHEDGLADTFDGFGGGWTAEDKLRIMKDSRLGSYGALALMLVLMLKWQLLVELALYDPVVAGSAMIVAHTVSRVVA
ASLIFTEKYVRDDESSKSKPLAQHQGINELFILIASGVLVLLVLKGIAALSLLLVMIGLRRLIVVIFRRQIGGYTGDTLG
AAQQICEIVCYFVLLVVGSIL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=247, Percent_Identity=35.2226720647773, Blast_Score=130, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 28675; Mature: 28543

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SERESWHKEIDLFLVAMGYFTRIPMPKWVEVDADKLNKASRYFGLVGLLVGLLSAIVFW
CCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTQNWLPAGVSVLLSMVTGVLLTGGFHEDGLADTFDGFGGGWTAEDKLRIMKDSRLGSYG
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHCCCCCHH
ALALMLVLMLKWQLLVELALYDPVVAGSAMIVAHTVSRVVAASLIFTEKYVRDDESSKSK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PLAQHQGINELFILIASGVLVLLVLKGIAALSLLLVMIGLRRLIVVIFRRQIGGYTGDTL
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
GAAQQICEIVCYFVLLVVGSIL
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA