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The map label for this gene is mlaE [H]

Identifier: 113969024

GI number: 113969024

Start: 788036

End: 788821

Strand: Direct

Name: mlaE [H]

Synonym: Shewmr4_0680

Alternate gene names: 113969024

Gene position: 788036-788821 (Clockwise)

Preceding gene: 113969023

Following gene: 113969025

Centisome position: 16.74

GC content: 54.71

Gene sequence:

>786_bases
GTGAAGTTATTTGACAGTATTGCCGACCTCGGCCGCAGCGCTATCGAGTTAGTGCTCGGTTTTGGCCGTGCGGGCCTGAT
GCTCTGGGGCGCGATTGTTCGCTGGCCTCGGGTACGTAAGGGGTTTCCATTATTAGTGCGCCAAATCTATGTGGTCGGCG
TACAATCTATGGTCATTATTGTGGTCTCGGGCCTGTTTATCGGCATGGTATTGGCGCTGCAGGGCTACAACATCTTAGTC
GGTTTCGGTACTGAGGAGAGCTTAGGCCCCATGGTGGCCTTAAGCTTACTGCGTGAACTCGGTCCTGTGGTCACCGCGCT
ATTATTTGCGGGCCGCGCCGGCTCGGCGCTGACCGCTGAAATCGGTTTGATGAAGAGTACCGAACAGTTATCCAGCTTAG
AGATGATGGCGATTGACCCGCTGCGGCAAATCATTGCGCCCCGTTTTTGGGCTGGGGTGATCAGCATGCCGCTGTTGGCG
CTGATGTTTACCGCGGTAGGCATTTATGGCGGCCATTTAGTGGGCGTGGAATGGAAAGGTATCGATGGCGGGGCTTTTTG
GTCGATTCTGCAAGCTTCGGTCGAATGGCGCCAAGACATAGTGAACTGCCTGATTAAGAGTGGCGTATTTGCCGTGGTGG
TGACTTGGATTGCCTTATACCGTGGCTATCAAGTGGTGCCGAATCCTGAGGGGATCAGCCGTGCAACCACCTCAACCGTG
GTGCAATCGAGCCTCGCCGTACTGGCATTGGACTTTTTACTAACAGCCCTGATGTTTGGCCGCTAA

Upstream 100 bases:

>100_bases
AGCTCAAACAGTTTATTCAAGGCGCGCCCGATGGTCCCGTGCCTTTCCACTATCCAGCAAAGAATTATCAGCAAGAATTA
ATGGCTAAAGGAGTTGGCAA

Downstream 100 bases:

>100_bases
CGGATTGCAGCGCCAAGGATACAGATAGACGGATTAACAGAGTGGTAACTGAGCATGTTGACACGAAAAATTGAATTATT
AGTGGGTGTGTTTCTCTTAG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MKLFDSIADLGRSAIELVLGFGRAGLMLWGAIVRWPRVRKGFPLLVRQIYVVGVQSMVIIVVSGLFIGMVLALQGYNILV
GFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKSTEQLSSLEMMAIDPLRQIIAPRFWAGVISMPLLA
LMFTAVGIYGGHLVGVEWKGIDGGAFWSILQASVEWRQDIVNCLIKSGVFAVVVTWIALYRGYQVVPNPEGISRATTSTV
VQSSLAVLALDFLLTALMFGR

Sequences:

>Translated_261_residues
MKLFDSIADLGRSAIELVLGFGRAGLMLWGAIVRWPRVRKGFPLLVRQIYVVGVQSMVIIVVSGLFIGMVLALQGYNILV
GFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKSTEQLSSLEMMAIDPLRQIIAPRFWAGVISMPLLA
LMFTAVGIYGGHLVGVEWKGIDGGAFWSILQASVEWRQDIVNCLIKSGVFAVVVTWIALYRGYQVVPNPEGISRATTSTV
VQSSLAVLALDFLLTALMFGR
>Mature_261_residues
MKLFDSIADLGRSAIELVLGFGRAGLMLWGAIVRWPRVRKGFPLLVRQIYVVGVQSMVIIVVSGLFIGMVLALQGYNILV
GFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKSTEQLSSLEMMAIDPLRQIIAPRFWAGVISMPLLA
LMFTAVGIYGGHLVGVEWKGIDGGAFWSILQASVEWRQDIVNCLIKSGVFAVVVTWIALYRGYQVVPNPEGISRATTSTV
VQSSLAVLALDFLLTALMFGR

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=259, Percent_Identity=69.1119691119691, Blast_Score=355, Evalue=1e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 28129; Mature: 28129

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFDSIADLGRSAIELVLGFGRAGLMLWGAIVRWPRVRKGFPLLVRQIYVVGVQSMVII
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHH
VVSGLFIGMVLALQGYNILVGFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAE
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IGLMKSTEQLSSLEMMAIDPLRQIIAPRFWAGVISMPLLALMFTAVGIYGGHLVGVEWKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEC
IDGGAFWSILQASVEWRQDIVNCLIKSGVFAVVVTWIALYRGYQVVPNPEGISRATTSTV
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHH
VQSSLAVLALDFLLTALMFGR
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLFDSIADLGRSAIELVLGFGRAGLMLWGAIVRWPRVRKGFPLLVRQIYVVGVQSMVII
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHH
VVSGLFIGMVLALQGYNILVGFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAE
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IGLMKSTEQLSSLEMMAIDPLRQIIAPRFWAGVISMPLLALMFTAVGIYGGHLVGVEWKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEC
IDGGAFWSILQASVEWRQDIVNCLIKSGVFAVVVTWIALYRGYQVVPNPEGISRATTSTV
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHH
VQSSLAVLALDFLLTALMFGR
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]