| Definition | Shewanella sp. MR-4 chromosome, complete genome. |
|---|---|
| Accession | NC_008321 |
| Length | 4,706,287 |
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The map label for this gene is mrcB [H]
Identifier: 113968968
GI number: 113968968
Start: 722630
End: 724924
Strand: Direct
Name: mrcB [H]
Synonym: Shewmr4_0624
Alternate gene names: 113968968
Gene position: 722630-724924 (Clockwise)
Preceding gene: 113968967
Following gene: 113968969
Centisome position: 15.35
GC content: 52.37
Gene sequence:
>2295_bases ATGACAACTAAGACGACAAAAAAAGCGAAAGCGAAGCGCAGCCGTGGCCTGTTAGGGCAAATGTGGTCGCTCACTTGGAA ACTAGCCTTAATCGGCCTAGCCGTTGTGACCTTCTACAGTATTTACCTCGATCAAATTATTGCCCGCAAGTTTGAGGGGC AAAAATGGCACTTACCCGCGCAGGTATTTAGCCGCTCGATGGCCTTATATCCCGGCGCTGCCGTAAGTCATCCACAGTTA ATGGCCGAACTCAAACTCTTAGGTTATCGCAAAGTTGCCAATCCCCGTGAGGTCGGTGAGTTCTCGGCATCCAGCACCAA AATCGAATTATGGCGTCGTCCCTTCTTACATCCTGAGGGCGATCAGGCCGAGCAGCGGGTGATGATTAGCTTCGACAGCG ATGGGATCAGCTCGATTGCGCGTATGTCAGATAAGCGCCAGTTGGCGATATTCCATTTAGAACCTGTCTTGCTCGACCGC ATTATTGCGGGTGACGGCGAAGACAGATTGTTTGTCCCCACAGAAGAAATGCCCAAGGCGATTGTAAAAGCCTTGCTGCT GGTGGAAGACCGCAGTTTTTACGAGCATCACGGGGTCAACCCCTTCGCGATTCTGCGCGCCGCTTTTGTGAATATCAGTG CGGGGCGAACTGTGCAGGGCGGCTCGACACTCACCCAGCAGTTGGCGAAAAACTTCTTCCTCTCGAGCGAACGCTCTTTG CTGCGTAAAATTCGCGAAGCCTTAATGGCGGTGATTATCGATTTTCGTTATAGCAAGGAAGAAATCCTCGAGGCCTACCT CAACGAAGTGTATATGGGGCAAGATAAATCCCGCGCCGTGCATGGGATGGGGCTGGCTTCACAGTTCTATTTTGGTCGAC CCATCGGCGAGCTAACCACGGCGCAGCAAGCGTTTTTAGTGGCGGCGATTAAGGGACCTTCCTATTACAACCCATGGCGT TATCCTGAGCGCAGCCAAGAGCGTCGCGATTTAGTGCTGCGTCTCTTGATGGAAGCGGGTGAACTGAGCACCGCGCAATA CAAGGTGGCGGTAGAGTCGCCCTTAGGCTTACGTAACGCCAATAAACCAGTGCATCAAAAGCTACCTGCATTTTATGCCT TGGTTAAACAAGAGCTTAACGAGCGCTATGGTGATGCGTTATTAAAACAATCTGGAGTGAAGATTTACACCACACTCGAT CCAATGGCGCAGGAAGCCGCCGAAATCGCCGTGACTCAAACCTTTAAGAGCTTAGATAAAGGCAATAAGTCGTTGCAGAT TGGTATGGTGGTTACAGACAAATACACTGGCGGTATCGCAGCTATGGTCGGTGATAAGACGCCAGGCTTTGATGGTTATA ACCGCGCGGTTGAGATCCGCAGACCCATAGGCTCGCTCGTCAAGCCATTTGTGTATGCGACCGCATTGGCACCGAATAGC CAGTTTACCTTGGCTTCGCCACTTAAAGATCAGCCGATTACCCTAAAGAATGAGCAGGGCAAAACTTGGTCACCGCAGAA CTTTGATAAGAAGTTTAGCGGACAAGTCTCGTTATTAACGGCGCTGAAAAAGTCGATGAACGTACCTACGGTGAACCTCG GGATTGCCGTGGGGGTGGATGCGGTGGCAACCACACTAGCGAAATCTGGCTGGCAAGAGCCGCTTAACGAATACCCCTCT ATGTTATTGGGCGCCGTGAATGGTTCGCCCTTGATGGTGGCGCAGGTTTATCAGACACTGGCCGATAGTGGCGCCTATCG TAAACTCACTACGGTCACGGCGGTGCTCGATAGTCACAATCAGCCATTACCTGTTACACGCTCACCGAAGGAGCAAGCCA TTACGCCGGATACTGATTTCTTGGTGCAATATGCTATGCAGCAAGTGGTGCGCTCGGGTACGGCAACACGTTTAGGCAAT GCCTTCCCAGGCGTAGCACTGGCGGGTAAAACCGGTACCAGTAACGATAGCCGCGACTCTTGGTTCGCGGGTTTTGATGA GCGTAACGTCGCGGCGATTTGGGTCGGACGCGACGATAACGGCAAGACCAGCCTCTACGGTAGCAGTGGCGCGATGGCGG TGTATCAAGCCTTCTTGAAGGAGCGTCCACCGATTGGGCTACGCTCGATTCCGCCAACGGGCGTGATCCAAGGTTACTTT GACCGTGATACCGGTGAAGCTAAGGAGGCGGGATGCCGCAATACCGAGGCATTGCCAGCCCTTAGAGGTACCTATAATCC TGCGAAAAACTGCGGTGAGCCGTTGCAGTGGTGGCAGAAGATTTTAGGTCAGTAA
Upstream 100 bases:
>100_bases CCTTAGGTTTATCTCAGTCATAGCCTTTAGACTGCTTTGATTTACAACCTTGTGTTGTGCCTGTGACTGATGCCGTTAAC GCATAAATGATGAAGTAGAT
Downstream 100 bases:
>100_bases TACTTATTCTTGATAGGTAAAGCGATAACAGCGGTGCAGCAAAATGGCACCGCTGTCTTTTAAAAAGAGAGTACCAGATG TATAAAACCGCAGCCGCAGT
Product: penicillin-binding protein 1B
Products: NA
Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]
Number of amino acids: Translated: 764; Mature: 763
Protein sequence:
>764_residues MTTKTTKKAKAKRSRGLLGQMWSLTWKLALIGLAVVTFYSIYLDQIIARKFEGQKWHLPAQVFSRSMALYPGAAVSHPQL MAELKLLGYRKVANPREVGEFSASSTKIELWRRPFLHPEGDQAEQRVMISFDSDGISSIARMSDKRQLAIFHLEPVLLDR IIAGDGEDRLFVPTEEMPKAIVKALLLVEDRSFYEHHGVNPFAILRAAFVNISAGRTVQGGSTLTQQLAKNFFLSSERSL LRKIREALMAVIIDFRYSKEEILEAYLNEVYMGQDKSRAVHGMGLASQFYFGRPIGELTTAQQAFLVAAIKGPSYYNPWR YPERSQERRDLVLRLLMEAGELSTAQYKVAVESPLGLRNANKPVHQKLPAFYALVKQELNERYGDALLKQSGVKIYTTLD PMAQEAAEIAVTQTFKSLDKGNKSLQIGMVVTDKYTGGIAAMVGDKTPGFDGYNRAVEIRRPIGSLVKPFVYATALAPNS QFTLASPLKDQPITLKNEQGKTWSPQNFDKKFSGQVSLLTALKKSMNVPTVNLGIAVGVDAVATTLAKSGWQEPLNEYPS MLLGAVNGSPLMVAQVYQTLADSGAYRKLTTVTAVLDSHNQPLPVTRSPKEQAITPDTDFLVQYAMQQVVRSGTATRLGN AFPGVALAGKTGTSNDSRDSWFAGFDERNVAAIWVGRDDNGKTSLYGSSGAMAVYQAFLKERPPIGLRSIPPTGVIQGYF DRDTGEAKEAGCRNTEALPALRGTYNPAKNCGEPLQWWQKILGQ
Sequences:
>Translated_764_residues MTTKTTKKAKAKRSRGLLGQMWSLTWKLALIGLAVVTFYSIYLDQIIARKFEGQKWHLPAQVFSRSMALYPGAAVSHPQL MAELKLLGYRKVANPREVGEFSASSTKIELWRRPFLHPEGDQAEQRVMISFDSDGISSIARMSDKRQLAIFHLEPVLLDR IIAGDGEDRLFVPTEEMPKAIVKALLLVEDRSFYEHHGVNPFAILRAAFVNISAGRTVQGGSTLTQQLAKNFFLSSERSL LRKIREALMAVIIDFRYSKEEILEAYLNEVYMGQDKSRAVHGMGLASQFYFGRPIGELTTAQQAFLVAAIKGPSYYNPWR YPERSQERRDLVLRLLMEAGELSTAQYKVAVESPLGLRNANKPVHQKLPAFYALVKQELNERYGDALLKQSGVKIYTTLD PMAQEAAEIAVTQTFKSLDKGNKSLQIGMVVTDKYTGGIAAMVGDKTPGFDGYNRAVEIRRPIGSLVKPFVYATALAPNS QFTLASPLKDQPITLKNEQGKTWSPQNFDKKFSGQVSLLTALKKSMNVPTVNLGIAVGVDAVATTLAKSGWQEPLNEYPS MLLGAVNGSPLMVAQVYQTLADSGAYRKLTTVTAVLDSHNQPLPVTRSPKEQAITPDTDFLVQYAMQQVVRSGTATRLGN AFPGVALAGKTGTSNDSRDSWFAGFDERNVAAIWVGRDDNGKTSLYGSSGAMAVYQAFLKERPPIGLRSIPPTGVIQGYF DRDTGEAKEAGCRNTEALPALRGTYNPAKNCGEPLQWWQKILGQ >Mature_763_residues TTKTTKKAKAKRSRGLLGQMWSLTWKLALIGLAVVTFYSIYLDQIIARKFEGQKWHLPAQVFSRSMALYPGAAVSHPQLM AELKLLGYRKVANPREVGEFSASSTKIELWRRPFLHPEGDQAEQRVMISFDSDGISSIARMSDKRQLAIFHLEPVLLDRI IAGDGEDRLFVPTEEMPKAIVKALLLVEDRSFYEHHGVNPFAILRAAFVNISAGRTVQGGSTLTQQLAKNFFLSSERSLL RKIREALMAVIIDFRYSKEEILEAYLNEVYMGQDKSRAVHGMGLASQFYFGRPIGELTTAQQAFLVAAIKGPSYYNPWRY PERSQERRDLVLRLLMEAGELSTAQYKVAVESPLGLRNANKPVHQKLPAFYALVKQELNERYGDALLKQSGVKIYTTLDP MAQEAAEIAVTQTFKSLDKGNKSLQIGMVVTDKYTGGIAAMVGDKTPGFDGYNRAVEIRRPIGSLVKPFVYATALAPNSQ FTLASPLKDQPITLKNEQGKTWSPQNFDKKFSGQVSLLTALKKSMNVPTVNLGIAVGVDAVATTLAKSGWQEPLNEYPSM LLGAVNGSPLMVAQVYQTLADSGAYRKLTTVTAVLDSHNQPLPVTRSPKEQAITPDTDFLVQYAMQQVVRSGTATRLGNA FPGVALAGKTGTSNDSRDSWFAGFDERNVAAIWVGRDDNGKTSLYGSSGAMAVYQAFLKERPPIGLRSIPPTGVIQGYFD RDTGEAKEAGCRNTEALPALRGTYNPAKNCGEPLQWWQKILGQ
Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal
COG id: COG0744
COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)
Gene ontology:
Cell location: Cell inner membrane; Single-pass type II membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI1786343, Length=736, Percent_Identity=43.6141304347826, Blast_Score=586, Evalue=1e-168, Organism=Escherichia coli, GI87082258, Length=260, Percent_Identity=38.8461538461538, Blast_Score=182, Evalue=6e-47, Organism=Escherichia coli, GI1788867, Length=539, Percent_Identity=30.7977736549165, Blast_Score=178, Evalue=1e-45, Organism=Escherichia coli, GI1789601, Length=170, Percent_Identity=31.1764705882353, Blast_Score=75, Evalue=1e-14,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011813 - InterPro: IPR001460 [H]
Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: =2.4.1.129 [H]
Molecular weight: Translated: 84327; Mature: 84196
Theoretical pI: Translated: 9.91; Mature: 9.91
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.3 %Cys (Translated Protein) 2.5 %Met (Translated Protein) 2.7 %Cys+Met (Translated Protein) 0.3 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 2.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTTKTTKKAKAKRSRGLLGQMWSLTWKLALIGLAVVTFYSIYLDQIIARKFEGQKWHLPA CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCH QVFSRSMALYPGAAVSHPQLMAELKLLGYRKVANPREVGEFSASSTKIELWRRPFLHPEG HHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHCCCCCCCC DQAEQRVMISFDSDGISSIARMSDKRQLAIFHLEPVLLDRIIAGDGEDRLFVPTEEMPKA CCCCCEEEEEECCCHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCCEEEECHHHHHHH IVKALLLVEDRSFYEHHGVNPFAILRAAFVNISAGRTVQGGSTLTQQLAKNFFLSSERSL HHHHHHHHHCCHHHHHCCCCHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHCCCHHHH LRKIREALMAVIIDFRYSKEEILEAYLNEVYMGQDKSRAVHGMGLASQFYFGRPIGELTT HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHCCCCHHHHHH AQQAFLVAAIKGPSYYNPWRYPERSQERRDLVLRLLMEAGELSTAQYKVAVESPLGLRNA HHHEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCC NKPVHQKLPAFYALVKQELNERYGDALLKQSGVKIYTTLDPMAQEAAEIAVTQTFKSLDK CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHC GNKSLQIGMVVTDKYTGGIAAMVGDKTPGFDGYNRAVEIRRPIGSLVKPFVYATALAPNS CCCEEEEEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC QFTLASPLKDQPITLKNEQGKTWSPQNFDKKFSGQVSLLTALKKSMNVPTVNLGIAVGVD CEEECCCCCCCCEEEECCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCEEEEHHHHHHH AVATTLAKSGWQEPLNEYPSMLLGAVNGSPLMVAQVYQTLADSGAYRKLTTVTAVLDSHN HHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC QPLPVTRSPKEQAITPDTDFLVQYAMQQVVRSGTATRLGNAFPGVALAGKTGTSNDSRDS CCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCEEECCCCCCCCCCCC WFAGFDERNVAAIWVGRDDNGKTSLYGSSGAMAVYQAFLKERPPIGLRSIPPTGVIQGYF CCCCCCCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCEECCC DRDTGEAKEAGCRNTEALPALRGTYNPAKNCGEPLQWWQKILGQ CCCCCCHHHCCCCCCCCCCHHHCCCCCHHHCCHHHHHHHHHHCC >Mature Secondary Structure TTKTTKKAKAKRSRGLLGQMWSLTWKLALIGLAVVTFYSIYLDQIIARKFEGQKWHLPA CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCH QVFSRSMALYPGAAVSHPQLMAELKLLGYRKVANPREVGEFSASSTKIELWRRPFLHPEG HHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHCCCCCCCC DQAEQRVMISFDSDGISSIARMSDKRQLAIFHLEPVLLDRIIAGDGEDRLFVPTEEMPKA CCCCCEEEEEECCCHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCCEEEECHHHHHHH IVKALLLVEDRSFYEHHGVNPFAILRAAFVNISAGRTVQGGSTLTQQLAKNFFLSSERSL HHHHHHHHHCCHHHHHCCCCHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHCCCHHHH LRKIREALMAVIIDFRYSKEEILEAYLNEVYMGQDKSRAVHGMGLASQFYFGRPIGELTT HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHCCCCHHHHHH AQQAFLVAAIKGPSYYNPWRYPERSQERRDLVLRLLMEAGELSTAQYKVAVESPLGLRNA HHHEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCC NKPVHQKLPAFYALVKQELNERYGDALLKQSGVKIYTTLDPMAQEAAEIAVTQTFKSLDK CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHC GNKSLQIGMVVTDKYTGGIAAMVGDKTPGFDGYNRAVEIRRPIGSLVKPFVYATALAPNS CCCEEEEEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC QFTLASPLKDQPITLKNEQGKTWSPQNFDKKFSGQVSLLTALKKSMNVPTVNLGIAVGVD CEEECCCCCCCCEEEECCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCEEEEHHHHHHH AVATTLAKSGWQEPLNEYPSMLLGAVNGSPLMVAQVYQTLADSGAYRKLTTVTAVLDSHN HHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC QPLPVTRSPKEQAITPDTDFLVQYAMQQVVRSGTATRLGNAFPGVALAGKTGTSNDSRDS CCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCEEECCCCCCCCCCCC WFAGFDERNVAAIWVGRDDNGKTSLYGSSGAMAVYQAFLKERPPIGLRSIPPTGVIQGYF CCCCCCCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCEECCC DRDTGEAKEAGCRNTEALPALRGTYNPAKNCGEPLQWWQKILGQ CCCCCCHHHCCCCCCCCCCHHHCCCCCHHHCCHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 10952301 [H]