| Definition | Shewanella sp. MR-4 chromosome, complete genome. |
|---|---|
| Accession | NC_008321 |
| Length | 4,706,287 |
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The map label for this gene is amiB [H]
Identifier: 113968939
GI number: 113968939
Start: 687264
End: 688652
Strand: Direct
Name: amiB [H]
Synonym: Shewmr4_0595
Alternate gene names: 113968939
Gene position: 687264-688652 (Clockwise)
Preceding gene: 113968938
Following gene: 113968940
Centisome position: 14.6
GC content: 48.96
Gene sequence:
>1389_bases ATGACTAAAAATAAGCATTACTTTCAGACTATTACTCTTTTTTTGTGCGCTTTTTTTGCTGTAGCAGCCCACGCGGCTAA CCAGCTTGAGGGCGTACGTATTTGGGCTGCCCCCGAGTCGACCCGTGTGGTGTTCGATTTGAGCGAAGCGCCTAATTACA CTTATTTTTCCCTCGATGGGCCCAATCGTTTGGTGGTCGACCTTAAAAAGGCTTCGACAAAACTGAGCCTGAAAAATATC GATAACAACAGTAAATTGGTTAAAGGGGTTCGCGTCAGCAAATCACCCACTAAAGGTGATTTACGTTTGGTGATCGATCT GGTTAAGCCATTAAATGCTAACCTGTTTTCTCTACCAGTGACGGCGCCCTATGGTAATCGTCTGGTGGTCGATCTCGAGG ATAAAACCTTAACCACGGCGACAGCTGTGGTGAGTTCAACTCCCGTTAAAACGGTTACTCAATCGGCGCAATCGTCCCGT GATATTGTGGTGGCCATTGATGCGGGTCATGGTGGTGATGATCCTGGTTCTATTGGGCCTTCAGGTGTTTACGAGAAAAA AGTCGCACTGGAAATAGCAAAAAGAGTGGCCAGTAAGATCAACGACACGCCGGGTATGCGCGCCGTGATGATCCGTACCG GCGATTACTTTGTGAATTTGAACAAGCGCTCCGAGCTAGCCCGGAACAGTAAGGCCGATTTACTGATTTCTATCCACGCC GATGCCTTTACCTCGCCTCAGCCAAAGGGGGCGTCTGTGTGGGTATTGTCGATGCGCCGCGCGAACAGTGAAATTGGTCG TTGGTTAGAGCAAAAAGAGAAACACTCAGAACTCTTAGGTGGTGCAGGGGAAATTATCCAAAATACCGATAATGAACAGT ACCTTGCAATGACTTTGCTCGACATGTCCATGAACAGTTCGATGGCCATAGGTCACTCTGTTGCTGGGGATATTCTCAAG GATCTCGGCTCGGTGACCGAGCTGCATAAGAGTCGCCCTGAGTCTGCGAGCCTTGCGGTATTAAAATCCCCCGATATTCC CTCCATTCTGGTGGAGACAGGATTTATCTCGAATCCGAAGGAAGAGCGGTTACTCAATAGCAGTCGCCATCAGGAAAGCA TTGCCACAGCTATCTACAAGGGCGTGAGCCGTTATTTCCACAATAATCCGCCGGCAGATACCCTGTTAGCGAAGCGTAAT AGTGGTAGTTCGACGACTAGAGCGAGCGCTAAATCCTCGAGCAATATTAAGCACAAGGTGAGCCGCGGTGAGTCACTTTC GGCAATAGCTCAACGTTATCAAGTGCCTATGGCCAGCATTAAGCGCGCCAATGGCATGAAGACGGATGTGGTGCAACTCG GACAAACACTCGTCATTCCTGAGAGTTAA
Upstream 100 bases:
>100_bases ACCTGCATTTAAATTATGTTAACCAAGGACGTGAAATCCAAATTCGAGCCTTAACAGAATCAGGTAAAAAGCTATTAGCC GCAATAAAATAAAAACAAAG
Downstream 100 bases:
>100_bases TAGGATGAAGTCTATGGGGATCCAGATCCTTCCGCCGCAACTAGCCAACCAAATCGCCGCCGGTGAGGTGGTCGAACGGC CTGCTTCCGTGGTGAAGGAA
Product: N-acetylmuramoyl-L-alanine amidase
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 462; Mature: 461
Protein sequence:
>462_residues MTKNKHYFQTITLFLCAFFAVAAHAANQLEGVRIWAAPESTRVVFDLSEAPNYTYFSLDGPNRLVVDLKKASTKLSLKNI DNNSKLVKGVRVSKSPTKGDLRLVIDLVKPLNANLFSLPVTAPYGNRLVVDLEDKTLTTATAVVSSTPVKTVTQSAQSSR DIVVAIDAGHGGDDPGSIGPSGVYEKKVALEIAKRVASKINDTPGMRAVMIRTGDYFVNLNKRSELARNSKADLLISIHA DAFTSPQPKGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLLDMSMNSSMAIGHSVAGDILK DLGSVTELHKSRPESASLAVLKSPDIPSILVETGFISNPKEERLLNSSRHQESIATAIYKGVSRYFHNNPPADTLLAKRN SGSSTTRASAKSSSNIKHKVSRGESLSAIAQRYQVPMASIKRANGMKTDVVQLGQTLVIPES
Sequences:
>Translated_462_residues MTKNKHYFQTITLFLCAFFAVAAHAANQLEGVRIWAAPESTRVVFDLSEAPNYTYFSLDGPNRLVVDLKKASTKLSLKNI DNNSKLVKGVRVSKSPTKGDLRLVIDLVKPLNANLFSLPVTAPYGNRLVVDLEDKTLTTATAVVSSTPVKTVTQSAQSSR DIVVAIDAGHGGDDPGSIGPSGVYEKKVALEIAKRVASKINDTPGMRAVMIRTGDYFVNLNKRSELARNSKADLLISIHA DAFTSPQPKGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLLDMSMNSSMAIGHSVAGDILK DLGSVTELHKSRPESASLAVLKSPDIPSILVETGFISNPKEERLLNSSRHQESIATAIYKGVSRYFHNNPPADTLLAKRN SGSSTTRASAKSSSNIKHKVSRGESLSAIAQRYQVPMASIKRANGMKTDVVQLGQTLVIPES >Mature_461_residues TKNKHYFQTITLFLCAFFAVAAHAANQLEGVRIWAAPESTRVVFDLSEAPNYTYFSLDGPNRLVVDLKKASTKLSLKNID NNSKLVKGVRVSKSPTKGDLRLVIDLVKPLNANLFSLPVTAPYGNRLVVDLEDKTLTTATAVVSSTPVKTVTQSAQSSRD IVVAIDAGHGGDDPGSIGPSGVYEKKVALEIAKRVASKINDTPGMRAVMIRTGDYFVNLNKRSELARNSKADLLISIHAD AFTSPQPKGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLLDMSMNSSMAIGHSVAGDILKD LGSVTELHKSRPESASLAVLKSPDIPSILVETGFISNPKEERLLNSSRHQESIATAIYKGVSRYFHNNPPADTLLAKRNS GSSTTRASAKSSSNIKHKVSRGESLSAIAQRYQVPMASIKRANGMKTDVVQLGQTLVIPES
Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]
COG id: COG0860
COG function: function code M; N-acetylmuramoyl-L-alanine amidase
Gene ontology:
Cell location: Periplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]
Homologues:
Organism=Escherichia coli, GI1790611, Length=425, Percent_Identity=37.1764705882353, Blast_Score=275, Evalue=5e-75, Organism=Escherichia coli, GI87082163, Length=396, Percent_Identity=34.8484848484849, Blast_Score=227, Evalue=1e-60, Organism=Escherichia coli, GI1788776, Length=225, Percent_Identity=43.1111111111111, Blast_Score=180, Evalue=2e-46,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002508 [H]
Pfam domain/function: PF01520 Amidase_3 [H]
EC number: =3.5.1.28 [H]
Molecular weight: Translated: 50054; Mature: 49922
Theoretical pI: Translated: 10.13; Mature: 10.13
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 2.4 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 2.0 %Met (Mature Protein) 2.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTKNKHYFQTITLFLCAFFAVAAHAANQLEGVRIWAAPESTRVVFDLSEAPNYTYFSLDG CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCEEEEEECC PNRLVVDLKKASTKLSLKNIDNNSKLVKGVRVSKSPTKGDLRLVIDLVKPLNANLFSLPV CCEEEEEEECCCCEEEEECCCCCCHHEECEEECCCCCCCCHHHHEEHHCCCCCCEEEEEE TAPYGNRLVVDLEDKTLTTATAVVSSTPVKTVTQSAQSSRDIVVAIDAGHGGDDPGSIGP ECCCCCEEEEEECCCCEEHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCC SGVYEKKVALEIAKRVASKINDTPGMRAVMIRTGDYFVNLNKRSELARNSKADLLISIHA CCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEECCHHHHHCCCCCCEEEEEEC DAFTSPQPKGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLL CCCCCCCCCCCEEEEEEEHHCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEEE DMSMNSSMAIGHSVAGDILKDLGSVTELHKSRPESASLAVLKSPDIPSILVETGFISNPK HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCH EERLLNSSRHQESIATAIYKGVSRYFHNNPPADTLLAKRNSGSSTTRASAKSSSNIKHKV HHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCCCCCCCCCCCCCHHHH SRGESLSAIAQRYQVPMASIKRANGMKTDVVQLGQTLVIPES HCCCHHHHHHHHHCCCHHHHHHCCCCHHHHHHCCCEEECCCC >Mature Secondary Structure TKNKHYFQTITLFLCAFFAVAAHAANQLEGVRIWAAPESTRVVFDLSEAPNYTYFSLDG CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCEEEEEECC PNRLVVDLKKASTKLSLKNIDNNSKLVKGVRVSKSPTKGDLRLVIDLVKPLNANLFSLPV CCEEEEEEECCCCEEEEECCCCCCHHEECEEECCCCCCCCHHHHEEHHCCCCCCEEEEEE TAPYGNRLVVDLEDKTLTTATAVVSSTPVKTVTQSAQSSRDIVVAIDAGHGGDDPGSIGP ECCCCCEEEEEECCCCEEHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCC SGVYEKKVALEIAKRVASKINDTPGMRAVMIRTGDYFVNLNKRSELARNSKADLLISIHA CCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEECCHHHHHCCCCCCEEEEEEC DAFTSPQPKGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLL CCCCCCCCCCCEEEEEEEHHCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEEE DMSMNSSMAIGHSVAGDILKDLGSVTELHKSRPESASLAVLKSPDIPSILVETGFISNPK HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCH EERLLNSSRHQESIATAIYKGVSRYFHNNPPADTLLAKRNSGSSTTRASAKSSSNIKHKV HHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCCCCCCCCCCCCCHHHH SRGESLSAIAQRYQVPMASIKRANGMKTDVVQLGQTLVIPES HCCCHHHHHHHHHCCCHHHHHHCCCCHHHHHHCCCEEECCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11677609; 2676972 [H]