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The map label for this gene is ampE [H]

Identifier: 113968770

GI number: 113968770

Start: 473097

End: 473948

Strand: Direct

Name: ampE [H]

Synonym: Shewmr4_0426

Alternate gene names: 113968770

Gene position: 473097-473948 (Clockwise)

Preceding gene: 113968769

Following gene: 113968771

Centisome position: 10.05

GC content: 48.71

Gene sequence:

>852_bases
ATGGCATTATTTTCATTACTGGTCGCCATTCTTGTGGAACGATTGAAGTTCCTTCCCGCCTCGTGGCAGAGCGATCGCGT
GCTGCAATCCTATCAATCGACTTTTTTTGGTGACAAAGCCTCGTTAACCAGTACCAGTATGTTGCTCGCTTTAGTGCTGC
CGGCTTTAGTGGTGCATATATTAGGTTGGGTTGCGTCAGGCATGTTTTGGGGGCTGTTAACTCTCGCCCTGTGGGTGGTA
GTGGCAATTATTTGCTTTAATCATCAAAAACAGCGCGATAGCTTTAAAAAGTATATGCAGGCGGCGTGTCGCTCCGACGT
GCAAGCCTGTTACCACTATGCCGCCGAATTAGATTGCAGTGAGTGTTTAGATACGGTGTCTGAAAAAGACTTAGGCGCCA
AGGTCGGGCAGAGTGTTGCCTGGATCAACTATCGCTACTATGGCGCCGTGGCATTATGCTTTATCTTCCTAGGCCCAGTT
GGCGCAGTCTTATATTGCACCGCGCGTTTTTACGCTGAAGAGAATATGCGAAAATCCCTCAACTTACCGTTAATTGACGA
CATTATGTTCGTTCTGGATTGGATCCCGAGTCGGATATTTTCCTTTGGCTATGCGCTCAGTGGTCAGTTTAGTGAAGGGC
TCACGGCTTGGCGTGACCATGCCTTAAATATCAATGCGAGTGCCAGAAGGGTGGTGACAGAAACGGCGTTAGCCTCCCAG
CCGCTGCCAGAGGAGTCGAGTGCACCAGTTTGTGTACAGTCGACCTTGGCATTATTAGTTTTAAGTAAACGCAACTTTAC
GCTGATGGTTGCGGTGCTGTCGCTGTTGACCATTTTTGGCCTAGTGACCTAA

Upstream 100 bases:

>100_bases
ATTTAACCGCGCTAGGCTAGCAGCAAAGAGGAGCAAGCGAGGGGGAGATATTTTCTCATCACCCTGAGCGTTTCCGGCTT
TTTTACAAGGAGTATTACCG

Downstream 100 bases:

>100_bases
CACATCTTCATTATTTAGGGTTAGGGCGTGTTGACGTTTCGAGATTAGATTTTGTTCGTTCTGGCAAGCACGTGCTCGCG
AAACGAGGAATGAAGTGTAG

Product: regulatory protein AmpE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MALFSLLVAILVERLKFLPASWQSDRVLQSYQSTFFGDKASLTSTSMLLALVLPALVVHILGWVASGMFWGLLTLALWVV
VAIICFNHQKQRDSFKKYMQAACRSDVQACYHYAAELDCSECLDTVSEKDLGAKVGQSVAWINYRYYGAVALCFIFLGPV
GAVLYCTARFYAEENMRKSLNLPLIDDIMFVLDWIPSRIFSFGYALSGQFSEGLTAWRDHALNINASARRVVTETALASQ
PLPEESSAPVCVQSTLALLVLSKRNFTLMVAVLSLLTIFGLVT

Sequences:

>Translated_283_residues
MALFSLLVAILVERLKFLPASWQSDRVLQSYQSTFFGDKASLTSTSMLLALVLPALVVHILGWVASGMFWGLLTLALWVV
VAIICFNHQKQRDSFKKYMQAACRSDVQACYHYAAELDCSECLDTVSEKDLGAKVGQSVAWINYRYYGAVALCFIFLGPV
GAVLYCTARFYAEENMRKSLNLPLIDDIMFVLDWIPSRIFSFGYALSGQFSEGLTAWRDHALNINASARRVVTETALASQ
PLPEESSAPVCVQSTLALLVLSKRNFTLMVAVLSLLTIFGLVT
>Mature_282_residues
ALFSLLVAILVERLKFLPASWQSDRVLQSYQSTFFGDKASLTSTSMLLALVLPALVVHILGWVASGMFWGLLTLALWVVV
AIICFNHQKQRDSFKKYMQAACRSDVQACYHYAAELDCSECLDTVSEKDLGAKVGQSVAWINYRYYGAVALCFIFLGPVG
AVLYCTARFYAEENMRKSLNLPLIDDIMFVLDWIPSRIFSFGYALSGQFSEGLTAWRDHALNINASARRVVTETALASQP
LPEESSAPVCVQSTLALLVLSKRNFTLMVAVLSLLTIFGLVT

Specific function: Putative signaling protein in beta-lactamase regulation. AmpE seems not to act as a direct sensor for beta-lactams [H]

COG id: COG3725

COG function: function code V; Membrane protein required for beta-lactamase induction

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786301, Length=295, Percent_Identity=27.1186440677966, Blast_Score=85, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: NA

Molecular weight: Translated: 31382; Mature: 31251

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALFSLLVAILVERLKFLPASWQSDRVLQSYQSTFFGDKASLTSTSMLLALVLPALVVHI
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LGWVASGMFWGLLTLALWVVVAIICFNHQKQRDSFKKYMQAACRSDVQACYHYAAELDCS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ECLDTVSEKDLGAKVGQSVAWINYRYYGAVALCFIFLGPVGAVLYCTARFYAEENMRKSL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NLPLIDDIMFVLDWIPSRIFSFGYALSGQFSEGLTAWRDHALNINASARRVVTETALASQ
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
PLPEESSAPVCVQSTLALLVLSKRNFTLMVAVLSLLTIFGLVT
CCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALFSLLVAILVERLKFLPASWQSDRVLQSYQSTFFGDKASLTSTSMLLALVLPALVVHI
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LGWVASGMFWGLLTLALWVVVAIICFNHQKQRDSFKKYMQAACRSDVQACYHYAAELDCS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ECLDTVSEKDLGAKVGQSVAWINYRYYGAVALCFIFLGPVGAVLYCTARFYAEENMRKSL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NLPLIDDIMFVLDWIPSRIFSFGYALSGQFSEGLTAWRDHALNINASARRVVTETALASQ
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
PLPEESSAPVCVQSTLALLVLSKRNFTLMVAVLSLLTIFGLVT
CCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2691840; 2607970; 9278503 [H]