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The map label for this gene is dapF [H]

Identifier: 113968736

GI number: 113968736

Start: 440307

End: 441134

Strand: Direct

Name: dapF [H]

Synonym: Shewmr4_0392

Alternate gene names: 113968736

Gene position: 440307-441134 (Clockwise)

Preceding gene: 113968735

Following gene: 113968737

Centisome position: 9.36

GC content: 51.45

Gene sequence:

>828_bases
TTGATCCAATTCACTAAGATGCACGGACTGGGCAACGATTTTATGGTGGTCGATGGTGTCACGCAGAACGTGTTCTTTTC
GCCTGAGCAGATCCGCCGTTTAGCCGACCGTAACTTTGGTGTCGGTTTCGATCAACTTTTACTGGTTGAGCCGCCCTATG
ATCCCGATTTGGATTTTCACTATCGTATCTTTAATGCCGACGGTGGTGAGGTCGAAAACTGCGGCAATGGCGCCCGTTGT
TTTGCGCGCTTTGTTCGTAATAAAGGGCTGACGAATAAGAATAAAATTCGTGTAAGTACCTCGGCGGGTAAGATGACTCT
GCGCCTCGAACGTGACGGTACTGTGACTGTGAATATGGGCGTGCCTGTGCTCGATCCTAGCCAAATTCCTTTCAAAGCCA
AGAAGGCCGAAAAAACCTATCTGCTGCAAACGTCGCAACAAACCTTTTTATGTGGCGCGGCGTCGATGGGTAACCCCCAT
TGCGTGCTCGATGTGGAAGATGTTGCTAACGCTAATGTCGCCGAGATTGGTGCACTGCTCACTAAGCATGAGCGTTTTCC
GCGCGGAGTAAACGTCGGCTTTATGCAGGTAGTGAACTCGGGCCATATCAAGCTTAGAGTTTATGAACGCGGTGCGGCCG
AGACTTTAGCCTGTGGTACTGGGGCTTGTGCCGCTGTGGTGGTTGGGCAAATCCAAGGCAAACTCGATCAACAGGTACGT
GTCGACTTGCCCGGGGGCACCTTAACCATCAATTGGGAAGGCGAAGGCAAACCCCTGTGGATGACGGGGCCAGCGCAGCA
CGTTTACGATGGACAAATTCAGTTATGA

Upstream 100 bases:

>100_bases
CTCTGGCAAGGTGAGCAACTCCTGCCGTAAACTAGAAAATTATATTGATTCAGGCGCTTGGCTAGTTAATGGCGGCGCCT
AGGCTTAAAGGATAAGTATC

Downstream 100 bases:

>100_bases
CCGAGCCTTTGATGCCGCAGACCGATCTTCCCTTCGATGAGTTGATTATTCGCGAGTACCTACTCGATAACCCAGACTTT
TTTAATCGTTATCCCGAGCT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MIQFTKMHGLGNDFMVVDGVTQNVFFSPEQIRRLADRNFGVGFDQLLLVEPPYDPDLDFHYRIFNADGGEVENCGNGARC
FARFVRNKGLTNKNKIRVSTSAGKMTLRLERDGTVTVNMGVPVLDPSQIPFKAKKAEKTYLLQTSQQTFLCGAASMGNPH
CVLDVEDVANANVAEIGALLTKHERFPRGVNVGFMQVVNSGHIKLRVYERGAAETLACGTGACAAVVVGQIQGKLDQQVR
VDLPGGTLTINWEGEGKPLWMTGPAQHVYDGQIQL

Sequences:

>Translated_275_residues
MIQFTKMHGLGNDFMVVDGVTQNVFFSPEQIRRLADRNFGVGFDQLLLVEPPYDPDLDFHYRIFNADGGEVENCGNGARC
FARFVRNKGLTNKNKIRVSTSAGKMTLRLERDGTVTVNMGVPVLDPSQIPFKAKKAEKTYLLQTSQQTFLCGAASMGNPH
CVLDVEDVANANVAEIGALLTKHERFPRGVNVGFMQVVNSGHIKLRVYERGAAETLACGTGACAAVVVGQIQGKLDQQVR
VDLPGGTLTINWEGEGKPLWMTGPAQHVYDGQIQL
>Mature_275_residues
MIQFTKMHGLGNDFMVVDGVTQNVFFSPEQIRRLADRNFGVGFDQLLLVEPPYDPDLDFHYRIFNADGGEVENCGNGARC
FARFVRNKGLTNKNKIRVSTSAGKMTLRLERDGTVTVNMGVPVLDPSQIPFKAKKAEKTYLLQTSQQTFLCGAASMGNPH
CVLDVEDVANANVAEIGALLTKHERFPRGVNVGFMQVVNSGHIKLRVYERGAAETLACGTGACAAVVVGQIQGKLDQQVR
VDLPGGTLTINWEGEGKPLWMTGPAQHVYDGQIQL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=69.3430656934307, Blast_Score=404, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 30084; Mature: 30084

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQFTKMHGLGNDFMVVDGVTQNVFFSPEQIRRLADRNFGVGFDQLLLVEPPYDPDLDFH
CEEEEEECCCCCCEEEEECCCCCEEECHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEE
YRIFNADGGEVENCGNGARCFARFVRNKGLTNKNKIRVSTSAGKMTLRLERDGTVTVNMG
EEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEECCCEEEEECC
VPVLDPSQIPFKAKKAEKTYLLQTSQQTFLCGAASMGNPHCVLDVEDVANANVAEIGALL
CCCCCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCCEEEEEHHHHCCCCHHHHHHHH
TKHERFPRGVNVGFMQVVNSGHIKLRVYERGAAETLACGTGACAAVVVGQIQGKLDQQVR
HHHHCCCCCCCCEEEEEECCCCEEEEEEECCCCCEEECCCCHHHHHHHHHHHCCCCCEEE
VDLPGGTLTINWEGEGKPLWMTGPAQHVYDGQIQL
EECCCCEEEEEECCCCCEEEEECCCCCCCCCEEEC
>Mature Secondary Structure
MIQFTKMHGLGNDFMVVDGVTQNVFFSPEQIRRLADRNFGVGFDQLLLVEPPYDPDLDFH
CEEEEEECCCCCCEEEEECCCCCEEECHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEE
YRIFNADGGEVENCGNGARCFARFVRNKGLTNKNKIRVSTSAGKMTLRLERDGTVTVNMG
EEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEECCCEEEEECC
VPVLDPSQIPFKAKKAEKTYLLQTSQQTFLCGAASMGNPHCVLDVEDVANANVAEIGALL
CCCCCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCCEEEEEHHHHCCCCHHHHHHHH
TKHERFPRGVNVGFMQVVNSGHIKLRVYERGAAETLACGTGACAAVVVGQIQGKLDQQVR
HHHHCCCCCCCCEEEEEECCCCEEEEEEECCCCCEEECCCCHHHHHHHHHHHCCCCCEEE
VDLPGGTLTINWEGEGKPLWMTGPAQHVYDGQIQL
EECCCCEEEEEECCCCCEEEEECCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA