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The map label for this gene is rep [H]

Identifier: 113968720

GI number: 113968720

Start: 401004

End: 403019

Strand: Direct

Name: rep [H]

Synonym: Shewmr4_0376

Alternate gene names: 113968720

Gene position: 401004-403019 (Clockwise)

Preceding gene: 113968716

Following gene: 113968733

Centisome position: 8.52

GC content: 49.95

Gene sequence:

>2016_bases
ATGAAACTCAATCCTGCCCAAAATGATGCTGTTCATTATGTTTCGGGTCCCTGCTTAGTGTTAGCGGGCGCGGGCAGTGG
CAAAACACGCGTAATTATCAACAAGATTGCCTACCTAGTGCAAAAGTGTGGCTACAATGCGCGCAATATTGCGGCGGTAA
CCTTTACCAACAAAGCGGCGCGGGAGATGAAGGAGCGGGTTGCGCAGTCGATGGGGCGCAAAGAGGCTAGAGGGCTGTGG
ATTTCGACCTTCCACACACTGGGGCTTGAGATTGTAAAACGTGAGCATAAAGTGCTGGGACTAAAGCCTGGGTTCTCGTT
ATTCGATGATCAAGACACGCTCGCGCTCCTTAAAGAGCTGACTCAAAAAGAATTACAGGAAGATAAAGACTTACTCAAAG
CTCTTGCGGGGGCAATTTCCAACTGGAAAGGTGGGCTGATTATTCCCGACCAAGCGCGCAAGTTGGCAAAGGATGAACAA
CAGCAGTTGTTTGCCATGTTGTATCAGCGTTATGCCCAACATATGAAAGCCTACAACGCCTTAGATTTTGATGACTTAAT
TCTGCTACCGACCTTATTGCTAAGGCATCACGAGGAAGTGCGTACGCGCTGGCAAACCAAGATCCAATACCTGCTGGTGG
ACGAATATCAGGATACCAACACTTCCCAGTATGAAATGGTCAAATTACTGGTGGGCGAGCGCGCGCGTTTTACCGTAGTG
GGGGACGACGACCAGTCCATCTATTCATGGCGCGGGGCTAAACCGCAGAACTTAGTGTTGTTGGGCAAAGATTTTCCCAA
GCTTAAGCTGATTAAACTTGAGCAGAATTATCGTTCTAGCCAACGTATTTTGCGGGCGGCTAACATCCTTATCGCCAACA
ATCCCCATGTGTACGATAAGTCGTTATTTAGCGAGTTGGCTTATGGTGAGCCGATGCGGGTGCTGTTTGCCGCCAATGAA
GAGCAAGAAGCCGAGCGTGTCGTGGCCGAAATCATTCGCCACAAGTTTGTTGGTCGTACCCAATTTGGTGATTACGCGAT
TCTGTATCGTGGGAACCACCAGTCTCGCCTGCTCGAACGGGCGCTGATGACTAACCGTATCCCCTACAAGCTAAGTGGCG
GCACCTCGTTTTTTGCCCGTGCCGAGATTAAAGACATCATGGGCTACCTCAAATTAGTGGTAAATCCCGATGATGATAAC
GCCTTTTTGCGAGTGGTAAATTTGCCTAAGCGCGGCATTGGTCCATCGACCTTAGAGCGTTTGGGTAACTTCTCCAACGA
GAAACATATTTCCATGTTTGAGGCGATTTTTGACCCTGAGCTGAATCATCATTTACCGCCTAATGCCATGTCGGCCTTGT
ATCAATTTGGCCGTTTTATTGTCGATACCGGGGAGCACGCCGAGCGAGGCGAACCAGTTGAGGCGGTAAAACAATTGATC
CGTAAGATTAATTACGAAGACTATTTATACGAGACCAGTACCAGCGCTAAGGCCGCCGAGATGCGGATGAAAAACATCTC
CGAGCTCTATCGCTGGGTGACGGAAATGCTCTCGGGGGATGAGTTAGAAGAACCCATGACCTTGCCTGAGGTGGTTAACC
GCCTGACGCTTAGGGATATGATGGAGCGTAACAGCGAGGATGAAGGCGGCGATCAAGTGCAGTTAATGACGCTGCATGCC
TCTAAAGGGTTGGAATTCCCCTTCGTGTTTATGGTGGGGGTTGAGGAGCGCATTCTGCCGCATCAAACCAGCATAGATGA
AGATAACGTCGACGAGGAACGTCGTTTGGCGTATGTGGGGATTACGCGTGCCCAGCGCGAGCTGTGGTTTACTCTTTGCC
GAGAGCGTCGCCAGTTTGGTGAAACCATGCGCTGCGAGCCGAGCCGTTTTCTGCATGAGTTACCGCAGGATGACCTTATT
TGGGAGAACCGTAAACCGCAGCAAACCGAACAGGAGCGCATGCAGACTGGCCGCAGCCATATCTCAAATTTAAGGGATAT
GTTTAAAAAGCAGTAA

Upstream 100 bases:

>100_bases
GGAGTAATAACGATTGCAAGGGGATTTGTTGGCCTTAAAACCTTAGCTTCTGCTATGATCCCATCCCCCTTCTGAAGACT
TGTTTGGATATTGCGCGAAG

Downstream 100 bases:

>100_bases
TCACAAGATTCATCATGTGATTACTGCATTCAGGCACAGACCTGTTGTGTGGTGTCTGACAATAAAAAAGGTGAGGCTAA
GGCACCGCCTTGGCCAAAGC

Product: ATP-dependent DNA helicase Rep

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 671; Mature: 671

Protein sequence:

>671_residues
MKLNPAQNDAVHYVSGPCLVLAGAGSGKTRVIINKIAYLVQKCGYNARNIAAVTFTNKAAREMKERVAQSMGRKEARGLW
ISTFHTLGLEIVKREHKVLGLKPGFSLFDDQDTLALLKELTQKELQEDKDLLKALAGAISNWKGGLIIPDQARKLAKDEQ
QQLFAMLYQRYAQHMKAYNALDFDDLILLPTLLLRHHEEVRTRWQTKIQYLLVDEYQDTNTSQYEMVKLLVGERARFTVV
GDDDQSIYSWRGAKPQNLVLLGKDFPKLKLIKLEQNYRSSQRILRAANILIANNPHVYDKSLFSELAYGEPMRVLFAANE
EQEAERVVAEIIRHKFVGRTQFGDYAILYRGNHQSRLLERALMTNRIPYKLSGGTSFFARAEIKDIMGYLKLVVNPDDDN
AFLRVVNLPKRGIGPSTLERLGNFSNEKHISMFEAIFDPELNHHLPPNAMSALYQFGRFIVDTGEHAERGEPVEAVKQLI
RKINYEDYLYETSTSAKAAEMRMKNISELYRWVTEMLSGDELEEPMTLPEVVNRLTLRDMMERNSEDEGGDQVQLMTLHA
SKGLEFPFVFMVGVEERILPHQTSIDEDNVDEERRLAYVGITRAQRELWFTLCRERRQFGETMRCEPSRFLHELPQDDLI
WENRKPQQTEQERMQTGRSHISNLRDMFKKQ

Sequences:

>Translated_671_residues
MKLNPAQNDAVHYVSGPCLVLAGAGSGKTRVIINKIAYLVQKCGYNARNIAAVTFTNKAAREMKERVAQSMGRKEARGLW
ISTFHTLGLEIVKREHKVLGLKPGFSLFDDQDTLALLKELTQKELQEDKDLLKALAGAISNWKGGLIIPDQARKLAKDEQ
QQLFAMLYQRYAQHMKAYNALDFDDLILLPTLLLRHHEEVRTRWQTKIQYLLVDEYQDTNTSQYEMVKLLVGERARFTVV
GDDDQSIYSWRGAKPQNLVLLGKDFPKLKLIKLEQNYRSSQRILRAANILIANNPHVYDKSLFSELAYGEPMRVLFAANE
EQEAERVVAEIIRHKFVGRTQFGDYAILYRGNHQSRLLERALMTNRIPYKLSGGTSFFARAEIKDIMGYLKLVVNPDDDN
AFLRVVNLPKRGIGPSTLERLGNFSNEKHISMFEAIFDPELNHHLPPNAMSALYQFGRFIVDTGEHAERGEPVEAVKQLI
RKINYEDYLYETSTSAKAAEMRMKNISELYRWVTEMLSGDELEEPMTLPEVVNRLTLRDMMERNSEDEGGDQVQLMTLHA
SKGLEFPFVFMVGVEERILPHQTSIDEDNVDEERRLAYVGITRAQRELWFTLCRERRQFGETMRCEPSRFLHELPQDDLI
WENRKPQQTEQERMQTGRSHISNLRDMFKKQ
>Mature_671_residues
MKLNPAQNDAVHYVSGPCLVLAGAGSGKTRVIINKIAYLVQKCGYNARNIAAVTFTNKAAREMKERVAQSMGRKEARGLW
ISTFHTLGLEIVKREHKVLGLKPGFSLFDDQDTLALLKELTQKELQEDKDLLKALAGAISNWKGGLIIPDQARKLAKDEQ
QQLFAMLYQRYAQHMKAYNALDFDDLILLPTLLLRHHEEVRTRWQTKIQYLLVDEYQDTNTSQYEMVKLLVGERARFTVV
GDDDQSIYSWRGAKPQNLVLLGKDFPKLKLIKLEQNYRSSQRILRAANILIANNPHVYDKSLFSELAYGEPMRVLFAANE
EQEAERVVAEIIRHKFVGRTQFGDYAILYRGNHQSRLLERALMTNRIPYKLSGGTSFFARAEIKDIMGYLKLVVNPDDDN
AFLRVVNLPKRGIGPSTLERLGNFSNEKHISMFEAIFDPELNHHLPPNAMSALYQFGRFIVDTGEHAERGEPVEAVKQLI
RKINYEDYLYETSTSAKAAEMRMKNISELYRWVTEMLSGDELEEPMTLPEVVNRLTLRDMMERNSEDEGGDQVQLMTLHA
SKGLEFPFVFMVGVEERILPHQTSIDEDNVDEERRLAYVGITRAQRELWFTLCRERRQFGETMRCEPSRFLHELPQDDLI
WENRKPQQTEQERMQTGRSHISNLRDMFKKQ

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=668, Percent_Identity=67.6646706586826, Blast_Score=917, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=638, Percent_Identity=38.5579937304075, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1787196, Length=346, Percent_Identity=28.3236994219653, Blast_Score=102, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6322369, Length=710, Percent_Identity=27.7464788732394, Blast_Score=215, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77495; Mature: 77495

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNPAQNDAVHYVSGPCLVLAGAGSGKTRVIINKIAYLVQKCGYNARNIAAVTFTNKAA
CCCCCCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHH
REMKERVAQSMGRKEARGLWISTFHTLGLEIVKREHKVLGLKPGFSLFDDQDTLALLKEL
HHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHH
TQKELQEDKDLLKALAGAISNWKGGLIIPDQARKLAKDEQQQLFAMLYQRYAQHMKAYNA
HHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LDFDDLILLPTLLLRHHEEVRTRWQTKIQYLLVDEYQDTNTSQYEMVKLLVGERARFTVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCCEEEEE
GDDDQSIYSWRGAKPQNLVLLGKDFPKLKLIKLEQNYRSSQRILRAANILIANNPHVYDK
CCCCCHHHHCCCCCCCCEEEECCCCCCEEEEEEHHHHHHHHHHHHHHCEEEECCCCCHHH
SLFSELAYGEPMRVLFAANEEQEAERVVAEIIRHKFVGRTQFGDYAILYRGNHQSRLLER
HHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHH
ALMTNRIPYKLSGGTSFFARAEIKDIMGYLKLVVNPDDDNAFLRVVNLPKRGIGPSTLER
HHHHCCCCEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCCEEEEEECCCCCCCHHHHHH
LGNFSNEKHISMFEAIFDPELNHHLPPNAMSALYQFGRFIVDTGEHAERGEPVEAVKQLI
HHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHH
RKINYEDYLYETSTSAKAAEMRMKNISELYRWVTEMLSGDELEEPMTLPEVVNRLTLRDM
HHCCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
MERNSEDEGGDQVQLMTLHASKGLEFPFVFMVGVEERILPHQTSIDEDNVDEERRLAYVG
HHCCCCCCCCCEEEEEEEECCCCCCCCEEEEECCHHHHCCCCCCCCCCCCCHHHHEEEEE
ITRAQRELWFTLCRERRQFGETMRCEPSRFLHELPQDDLIWENRKPQQTEQERMQTGRSH
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
ISNLRDMFKKQ
HHHHHHHHHCC
>Mature Secondary Structure
MKLNPAQNDAVHYVSGPCLVLAGAGSGKTRVIINKIAYLVQKCGYNARNIAAVTFTNKAA
CCCCCCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHH
REMKERVAQSMGRKEARGLWISTFHTLGLEIVKREHKVLGLKPGFSLFDDQDTLALLKEL
HHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHH
TQKELQEDKDLLKALAGAISNWKGGLIIPDQARKLAKDEQQQLFAMLYQRYAQHMKAYNA
HHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LDFDDLILLPTLLLRHHEEVRTRWQTKIQYLLVDEYQDTNTSQYEMVKLLVGERARFTVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCCEEEEE
GDDDQSIYSWRGAKPQNLVLLGKDFPKLKLIKLEQNYRSSQRILRAANILIANNPHVYDK
CCCCCHHHHCCCCCCCCEEEECCCCCCEEEEEEHHHHHHHHHHHHHHCEEEECCCCCHHH
SLFSELAYGEPMRVLFAANEEQEAERVVAEIIRHKFVGRTQFGDYAILYRGNHQSRLLER
HHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHH
ALMTNRIPYKLSGGTSFFARAEIKDIMGYLKLVVNPDDDNAFLRVVNLPKRGIGPSTLER
HHHHCCCCEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCCEEEEEECCCCCCCHHHHHH
LGNFSNEKHISMFEAIFDPELNHHLPPNAMSALYQFGRFIVDTGEHAERGEPVEAVKQLI
HHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHH
RKINYEDYLYETSTSAKAAEMRMKNISELYRWVTEMLSGDELEEPMTLPEVVNRLTLRDM
HHCCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
MERNSEDEGGDQVQLMTLHASKGLEFPFVFMVGVEERILPHQTSIDEDNVDEERRLAYVG
HHCCCCCCCCCEEEEEEEECCCCCCCCEEEEECCHHHHCCCCCCCCCCCCCHHHHEEEEE
ITRAQRELWFTLCRERRQFGETMRCEPSRFLHELPQDDLIWENRKPQQTEQERMQTGRSH
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
ISNLRDMFKKQ
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]