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The map label for this gene is tuf1 [H]

Identifier: 113968542

GI number: 113968542

Start: 227874

End: 229058

Strand: Direct

Name: tuf1 [H]

Synonym: Shewmr4_0197

Alternate gene names: 113968542

Gene position: 227874-229058 (Clockwise)

Preceding gene: 113968541

Following gene: 113968543

Centisome position: 4.84

GC content: 47.76

Gene sequence:

>1185_bases
GTGGCAAAAGCTAAATTTGAACGTAATAAGCCTCACGTAAACGTGGGCACCATCGGTCACGTTGACCATGGTAAAACCAC
TCTGACTGCAGCTATCTCTCACGTACTGGCTAAGACCTACGGTGGTGAAGCTAAAGACTTCTCTCAAATCGATAACGCTC
CAGAAGAGCGTGAGCGCGGTATTACCATCAATACCTCTCACATCGAATACGATACACCAACTCGTCACTACGCACACGTA
GACTGCCCAGGCCACGCTGACTATGTTAAAAACATGATCACTGGTGCTGCACAGATGGACGGCGCGATCCTGGTAGTAGC
TTCTACTGACGGTCCAATGCCACAAACTCGTGAGCACATCCTGCTGTCTCGCCAAGTAGGCGTACCTTTCATCATCGTAT
TCATGAACAAGTGTGACATGGTTGACGATGCTGAACTGTTAGAATTAGTAGAAATGGAAGTACGTGAACTGTTATCAGAA
TACGACTTCCCAGGTGATGACTTACCAGTTATCCAAGGTTCTGCTCTGAAAGCGCTAGAAGGCGAGCCAGAGTGGGAAGC
TAAGATCATCGAATTAGCAGCGGCACTGGATTCTTACATTCCAGAACCAGAGCGTGATATCGATAAGCCATTCCTGATGC
CAATCGAAGACGTATTCTCAATCTCTGGCCGTGGTACAGTAGTAACTGGCCGTGTTGAGCGTGGTATCGTACGTGTTGGT
GACGAAGTAGAAATCGTTGGTATCCGTACAACGACTAAGACAACCTGTACTGGTGTTGAAATGTTCCGTAAGCTGCTTGA
CGAAGGTCGTGCAGGTGAGAACTGTGGTATCCTGTTACGTGGTACTAAGCGTGATGACGTAGAACGTGGTCAAGTATTAT
CTAAGCCAGGTTCAATCAACCCACACACTACTTTTGAATCAGAAGTATACGTTCTGTCAAAAGAAGAAGGTGGTCGTCAC
ACTCCATTCTTCAAAGGCTACCGTCCACAGTTCTACTTCCGTACAACTGACGTGACTGGTACCATCGAACTGCCAGAAGG
CGTAGAGATGGTAATGCCAGGCGACAACATCAAGATGGTTGTTACGCTGATCTGCCCAATCGCGATGGACGAAGGTTTAC
GCTTCGCAATCCGTGAAGGTGGCCGTACAGTAGGTGCAGGTGTTGTAGCTAAGATCATCGCTTAA

Upstream 100 bases:

>100_bases
CGAAAGCGATTATTGAATCTCGTAGCTAATTTCAAGTTACGACATAAATGTAATATGGTCCTGTTGATACAGGATTATTC
TGAAAAGAAAGGAATATATC

Downstream 100 bases:

>100_bases
TTGCGAACTTAGATATAGTACTGAAAAAGGAAGCTTAGGCTTCCTTTTTTATTTTGGGCTGGCGTTATTCTGAAATTTGT
GTAAAATGCTCGCCACTCTG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAKAKFERNKPHVNVGTIGHVDHGKTTLTAAISHVLAKTYGGEAKDFSQIDNAPEERERGITINTSHIEYDTPTRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHILLSRQVGVPFIIVFMNKCDMVDDAELLELVEMEVRELLSE
YDFPGDDLPVIQGSALKALEGEPEWEAKIIELAAALDSYIPEPERDIDKPFLMPIEDVFSISGRGTVVTGRVERGIVRVG
DEVEIVGIRTTTKTTCTGVEMFRKLLDEGRAGENCGILLRGTKRDDVERGQVLSKPGSINPHTTFESEVYVLSKEEGGRH
TPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLICPIAMDEGLRFAIREGGRTVGAGVVAKIIA

Sequences:

>Translated_394_residues
MAKAKFERNKPHVNVGTIGHVDHGKTTLTAAISHVLAKTYGGEAKDFSQIDNAPEERERGITINTSHIEYDTPTRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHILLSRQVGVPFIIVFMNKCDMVDDAELLELVEMEVRELLSE
YDFPGDDLPVIQGSALKALEGEPEWEAKIIELAAALDSYIPEPERDIDKPFLMPIEDVFSISGRGTVVTGRVERGIVRVG
DEVEIVGIRTTTKTTCTGVEMFRKLLDEGRAGENCGILLRGTKRDDVERGQVLSKPGSINPHTTFESEVYVLSKEEGGRH
TPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLICPIAMDEGLRFAIREGGRTVGAGVVAKIIA
>Mature_393_residues
AKAKFERNKPHVNVGTIGHVDHGKTTLTAAISHVLAKTYGGEAKDFSQIDNAPEERERGITINTSHIEYDTPTRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHILLSRQVGVPFIIVFMNKCDMVDDAELLELVEMEVRELLSEY
DFPGDDLPVIQGSALKALEGEPEWEAKIIELAAALDSYIPEPERDIDKPFLMPIEDVFSISGRGTVVTGRVERGIVRVGD
EVEIVGIRTTTKTTCTGVEMFRKLLDEGRAGENCGILLRGTKRDDVERGQVLSKPGSINPHTTFESEVYVLSKEEGGRHT
PFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLICPIAMDEGLRFAIREGGRTVGAGVVAKIIA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=56.7088607594937, Blast_Score=472, Evalue=1e-133,
Organism=Homo sapiens, GI4503475, Length=356, Percent_Identity=32.8651685393258, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI223555963, Length=443, Percent_Identity=29.1196388261851, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI5729864, Length=443, Percent_Identity=29.1196388261851, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI4503471, Length=449, Percent_Identity=28.2850779510022, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI54607086, Length=345, Percent_Identity=28.9855072463768, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI194018522, Length=343, Percent_Identity=29.4460641399417, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI194018520, Length=343, Percent_Identity=29.4460641399417, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI194097354, Length=343, Percent_Identity=29.4460641399417, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI46094014, Length=345, Percent_Identity=29.2753623188406, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI4503507, Length=368, Percent_Identity=28.2608695652174, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI53729339, Length=232, Percent_Identity=31.0344827586207, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI53729337, Length=232, Percent_Identity=31.0344827586207, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI157426893, Length=250, Percent_Identity=27.2, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=86.5482233502538, Blast_Score=688, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=86.5482233502538, Blast_Score=687, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=377, Percent_Identity=29.1777188328912, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI48994988, Length=252, Percent_Identity=30.5555555555556, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789108, Length=322, Percent_Identity=27.639751552795, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=51.6290726817043, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25141371, Length=386, Percent_Identity=46.1139896373057, Blast_Score=343, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI17552884, Length=450, Percent_Identity=29.5555555555556, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17569207, Length=450, Percent_Identity=29.5555555555556, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI32566303, Length=435, Percent_Identity=28.0459770114943, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI115532067, Length=436, Percent_Identity=27.2935779816514, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=436, Percent_Identity=27.2935779816514, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17506081, Length=271, Percent_Identity=30.6273062730627, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI32566629, Length=342, Percent_Identity=27.7777777777778, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=35.7142857142857, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=447, Percent_Identity=25.9507829977629, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=235, Percent_Identity=28.5106382978723, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=63.727959697733, Blast_Score=520, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6325337, Length=447, Percent_Identity=30.4250559284116, Blast_Score=153, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=447, Percent_Identity=30.4250559284116, Blast_Score=153, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6322937, Length=460, Percent_Identity=22.8260869565217, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320377, Length=337, Percent_Identity=28.7833827893175, Blast_Score=90, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=291, Percent_Identity=27.8350515463918, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=269, Percent_Identity=30.4832713754647, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=56.7430025445293, Blast_Score=470, Evalue=1e-133,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=56.7430025445293, Blast_Score=470, Evalue=1e-133,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=51.2953367875648, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI45553807, Length=449, Percent_Identity=27.8396436525612, Blast_Score=154, Evalue=9e-38,
Organism=Drosophila melanogaster, GI45553816, Length=449, Percent_Identity=27.8396436525612, Blast_Score=154, Evalue=9e-38,
Organism=Drosophila melanogaster, GI24651721, Length=449, Percent_Identity=27.8396436525612, Blast_Score=154, Evalue=9e-38,
Organism=Drosophila melanogaster, GI17864154, Length=449, Percent_Identity=27.8396436525612, Blast_Score=154, Evalue=9e-38,
Organism=Drosophila melanogaster, GI24652838, Length=451, Percent_Identity=28.3813747228381, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI17137572, Length=451, Percent_Identity=28.3813747228381, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI19922690, Length=302, Percent_Identity=29.8013245033113, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI45550900, Length=437, Percent_Identity=28.1464530892449, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17137380, Length=335, Percent_Identity=31.3432835820896, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24647048, Length=417, Percent_Identity=25.8992805755396, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24647046, Length=417, Percent_Identity=25.8992805755396, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28572034, Length=226, Percent_Identity=32.7433628318584, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43326; Mature: 43195

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERNKPHVNVGTIGHVDHGKTTLTAAISHVLAKTYGGEAKDFSQIDNAPEERERG
CCCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHCC
ITINTSHIEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHI
EEEEECEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLSRQVGVPFIIVFMNKCDMVDDAELLELVEMEVRELLSEYDFPGDDLPVIQGSALKALE
EEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHCC
GEPEWEAKIIELAAALDSYIPEPERDIDKPFLMPIEDVFSISGRGTVVTGRVERGIVRVG
CCCCHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHCCCCCCEEEECHHCCCEEEEC
DEVEIVGIRTTTKTTCTGVEMFRKLLDEGRAGENCGILLRGTKRDDVERGQVLSKPGSIN
CEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHCCEECCCCCCC
PHTTFESEVYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMV
CCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCCEEE
VTLICPIAMDEGLRFAIREGGRTVGAGVVAKIIA
EEEHHHHHHCCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKAKFERNKPHVNVGTIGHVDHGKTTLTAAISHVLAKTYGGEAKDFSQIDNAPEERERG
CCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHCC
ITINTSHIEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHI
EEEEECEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLSRQVGVPFIIVFMNKCDMVDDAELLELVEMEVRELLSEYDFPGDDLPVIQGSALKALE
EEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHCC
GEPEWEAKIIELAAALDSYIPEPERDIDKPFLMPIEDVFSISGRGTVVTGRVERGIVRVG
CCCCHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHCCCCCCEEEECHHCCCEEEEC
DEVEIVGIRTTTKTTCTGVEMFRKLLDEGRAGENCGILLRGTKRDDVERGQVLSKPGSIN
CEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHCCEECCCCCCC
PHTTFESEVYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMV
CCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCCEEE
VTLICPIAMDEGLRFAIREGGRTVGAGVVAKIIA
EEEHHHHHHCCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA