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The map label for this gene is mscM [H]

Identifier: 113968462

GI number: 113968462

Start: 129392

End: 130660

Strand: Direct

Name: mscM [H]

Synonym: Shewmr4_0117

Alternate gene names: 113968462

Gene position: 129392-130660 (Clockwise)

Preceding gene: 113968461

Following gene: 113968465

Centisome position: 2.75

GC content: 50.59

Gene sequence:

>1269_bases
GTGGATCAAGAAATTCGCCTTCAAATCTCGAACTGGCTATCGAGTATCGGGATCGACAGCCAACCATCCGACGGCATATC
GACCAGCATTATGCTGCTTGCCTGTGTGCTGGTGGCGGCCATTGCCTATTTTATTATGCGCCGCGGGGTGATCCGTGCGG
TCAACATGGTGATCCTGCGCTCCAAGGCGACGTGGGACGACGTATTTATGCGTTACCGGGTGCTGGAAAAGTTGGCAATG
TTAGTGCCTGCTATCGTGCTAAACCTTCTGGTGCCTATAACCTTAACCGAGCATCCCGTACTCAGCAGTTTAGTCGACCG
TCTATTGAGTATTTGGTTGGTGATCCTGATGATCCGCGCCATTTACGCAGGGCTCGATGCGGTTAATGAGATTTCCGATG
TCAATTTGGTCAGCCGCCGCTTGCCGGTAAAAAGCTTTGTGCAGCTGATTAAACTGTTTCTGTTCTTTGTCGGTCTGATC
GTGTCGATTTCTGTACTGGCGGATCAATCACCCGTGTACTTCCTCAGCGGTTTAGGTGTGGCAACGGGTTTTGTGATGTT
AGTGTTCCGCGACACCATTTTAGGCTTCGTGGCGGGGATCCAGCTGGCGGCTAACCGTATGGTCAGCAAGGGCGACTGGA
TCCAGATGGACAAATACGGCGCCGACGGTGCGGTGGAGGAAGTGTCCTTAACTACGGTCAAAGTGCGTAACTGGGACAAG
ACCATCACTATGATCCCCGCCTATGCCTTAGTGTCGGATGCGTTTCGTAACTGGCGAGGTATGTCGGAGTCGGGCGGTCG
CCGTATCAAGCGTGCGGTCAATATCGATATCAACAGCATTAAGTTTTTAACCGAGGAAGAGCGTAGTCGACTCAGTAAAA
TCAATTGCTTGAAAGAATATTTCCCTGCCAAGATCAGTGAGATCCAAGAGTCGAATGCCAAAGTGTCGGATCTGGATATG
AAGGTCAATGGCCGCCATCTGACCAACGTTGGTACCTTCCGCGCTTATTTGCAGGAGTACCTGCAACGCCATGATAAAGT
GCACAAAGATATGACACTGATGGTGCGCCAACTGGCTCCAACGACCGAAGGTTTGCCGATTGAGATTTATATCTTTACCA
ATGATACACGTTGGGCCTTTTATGAGGCGATACAAGCGGATATCTTCGATCATATCTTTGCCGTGTTGCCCGAATTTGGT
TTACAGGCCTTCCAAGCGCCGACGGGCAACGATATCCGCAGTCTAAAATCGGTGAAAGTCGAGGGCTAA

Upstream 100 bases:

>100_bases
CAGATGCCGCAGTCGCACGTCATGTGCTCGCAACCTTAAGCCCTTATCTTGGCATGGCTGACCTGGGCAGATAACCCAGC
CGTTTTTCTAGGAGTAGATT

Downstream 100 bases:

>100_bases
TCCCGACTGACTAAGATGGCGGCGAGGGTGAGAAACACGCCGCCGCTGGTACGGTCAAACCAATGCAGTTTGTTGCTGGC
CTTTAGGCTCGGGGCCAGCA

Product: mechanosensitive ion channel protein MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MDQEIRLQISNWLSSIGIDSQPSDGISTSIMLLACVLVAAIAYFIMRRGVIRAVNMVILRSKATWDDVFMRYRVLEKLAM
LVPAIVLNLLVPITLTEHPVLSSLVDRLLSIWLVILMIRAIYAGLDAVNEISDVNLVSRRLPVKSFVQLIKLFLFFVGLI
VSISVLADQSPVYFLSGLGVATGFVMLVFRDTILGFVAGIQLAANRMVSKGDWIQMDKYGADGAVEEVSLTTVKVRNWDK
TITMIPAYALVSDAFRNWRGMSESGGRRIKRAVNIDINSIKFLTEEERSRLSKINCLKEYFPAKISEIQESNAKVSDLDM
KVNGRHLTNVGTFRAYLQEYLQRHDKVHKDMTLMVRQLAPTTEGLPIEIYIFTNDTRWAFYEAIQADIFDHIFAVLPEFG
LQAFQAPTGNDIRSLKSVKVEG

Sequences:

>Translated_422_residues
MDQEIRLQISNWLSSIGIDSQPSDGISTSIMLLACVLVAAIAYFIMRRGVIRAVNMVILRSKATWDDVFMRYRVLEKLAM
LVPAIVLNLLVPITLTEHPVLSSLVDRLLSIWLVILMIRAIYAGLDAVNEISDVNLVSRRLPVKSFVQLIKLFLFFVGLI
VSISVLADQSPVYFLSGLGVATGFVMLVFRDTILGFVAGIQLAANRMVSKGDWIQMDKYGADGAVEEVSLTTVKVRNWDK
TITMIPAYALVSDAFRNWRGMSESGGRRIKRAVNIDINSIKFLTEEERSRLSKINCLKEYFPAKISEIQESNAKVSDLDM
KVNGRHLTNVGTFRAYLQEYLQRHDKVHKDMTLMVRQLAPTTEGLPIEIYIFTNDTRWAFYEAIQADIFDHIFAVLPEFG
LQAFQAPTGNDIRSLKSVKVEG
>Mature_422_residues
MDQEIRLQISNWLSSIGIDSQPSDGISTSIMLLACVLVAAIAYFIMRRGVIRAVNMVILRSKATWDDVFMRYRVLEKLAM
LVPAIVLNLLVPITLTEHPVLSSLVDRLLSIWLVILMIRAIYAGLDAVNEISDVNLVSRRLPVKSFVQLIKLFLFFVGLI
VSISVLADQSPVYFLSGLGVATGFVMLVFRDTILGFVAGIQLAANRMVSKGDWIQMDKYGADGAVEEVSLTTVKVRNWDK
TITMIPAYALVSDAFRNWRGMSESGGRRIKRAVNIDINSIKFLTEEERSRLSKINCLKEYFPAKISEIQESNAKVSDLDM
KVNGRHLTNVGTFRAYLQEYLQRHDKVHKDMTLMVRQLAPTTEGLPIEIYIFTNDTRWAFYEAIQADIFDHIFAVLPEFG
LQAFQAPTGNDIRSLKSVKVEG

Specific function: Mechanosensitive channel of miniconductance that confers protection against mild hypoosmotic shock. Overexpression confers protection against severe shocks [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786791, Length=393, Percent_Identity=45.5470737913486, Blast_Score=360, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 47519; Mature: 47519

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQEIRLQISNWLSSIGIDSQPSDGISTSIMLLACVLVAAIAYFIMRRGVIRAVNMVILR
CCCHHEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SKATWDDVFMRYRVLEKLAMLVPAIVLNLLVPITLTEHPVLSSLVDRLLSIWLVILMIRA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
IYAGLDAVNEISDVNLVSRRLPVKSFVQLIKLFLFFVGLIVSISVLADQSPVYFLSGLGV
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ATGFVMLVFRDTILGFVAGIQLAANRMVSKGDWIQMDKYGADGAVEEVSLTTVKVRNWDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHEEEEEEEEECCCC
TITMIPAYALVSDAFRNWRGMSESGGRRIKRAVNIDINSIKFLTEEERSRLSKINCLKEY
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
FPAKISEIQESNAKVSDLDMKVNGRHLTNVGTFRAYLQEYLQRHDKVHKDMTLMVRQLAP
HHHHHHHHHHCCCCEEECEEEECCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TTEGLPIEIYIFTNDTRWAFYEAIQADIFDHIFAVLPEFGLQAFQAPTGNDIRSLKSVKV
CCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCC
EG
CC
>Mature Secondary Structure
MDQEIRLQISNWLSSIGIDSQPSDGISTSIMLLACVLVAAIAYFIMRRGVIRAVNMVILR
CCCHHEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SKATWDDVFMRYRVLEKLAMLVPAIVLNLLVPITLTEHPVLSSLVDRLLSIWLVILMIRA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
IYAGLDAVNEISDVNLVSRRLPVKSFVQLIKLFLFFVGLIVSISVLADQSPVYFLSGLGV
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ATGFVMLVFRDTILGFVAGIQLAANRMVSKGDWIQMDKYGADGAVEEVSLTTVKVRNWDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHEEEEEEEEECCCC
TITMIPAYALVSDAFRNWRGMSESGGRRIKRAVNIDINSIKFLTEEERSRLSKINCLKEY
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
FPAKISEIQESNAKVSDLDMKVNGRHLTNVGTFRAYLQEYLQRHDKVHKDMTLMVRQLAP
HHHHHHHHHHCCCCEEECEEEECCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TTEGLPIEIYIFTNDTRWAFYEAIQADIFDHIFAVLPEFGLQAFQAPTGNDIRSLKSVKV
CCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCC
EG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 7813889 [H]