| Definition | Shewanella sp. MR-4 chromosome, complete genome. |
|---|---|
| Accession | NC_008321 |
| Length | 4,706,287 |
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The map label for this gene is dnaN [H]
Identifier: 113968348
GI number: 113968348
Start: 1482
End: 2582
Strand: Direct
Name: dnaN [H]
Synonym: Shewmr4_0002
Alternate gene names: 113968348
Gene position: 1482-2582 (Clockwise)
Preceding gene: 113968347
Following gene: 113968349
Centisome position: 0.03
GC content: 45.96
Gene sequence:
>1101_bases ATGAAATTTTCAATTGATAGGGACGCCCTATTAAAGCCGCTTCAATTGGTTTGTGGTGCGGTAGAAAGACGCCATAACTT GCCTATTTTGGCAAATCTCCTTGTAGAAGTTAGTGGTCACTCACTCAAAATGACAGGTACTGACCTCGAAGTTGAGTTAG TGGGCCAGGCGGTCATTCATGGTGATATCGAAGAAGGTCGTACTACAGTTCCAGCTAAGAAGCTGTTAGACATAGTTAAA TCCTTACCCGAGCAAAGCGAGCTTAAGGTCGAGCAACAAGATAACAAATGGTTGTTACGCTCTGGCCGTAGCCGTTTCTC GCTGGCAACCCTGCCCGCTGAAGAATACCCCAATGTGGAAGCCTTTCAGGCGGAAATCGAATTTACCCTTAAGCAAGGCG TGCTGAAGTCACTGATTGATGCGACTCAGTTTTCGATGGCGAACCAAGACGTTCGTTACTATCTGAATGGTTTATTGCTC GAAACCGAAGGGAATGTGCTGCGCGCCATTGCTACCGACGGTCATCGCTTAGCCTTAAGTCACAGAACCATAGATGCGCA ATTACCTGAAAAACAAGTGATCGTGCCCCGTAAAGGCGTGATGGAAATGGCGCGTTTGTTGGAAGCCGATGATTTAGATA TCGCTATTGCCATTGGTGATAATGCTATTCGTGCGACCACCAGCACCACAGTCTTTACCAGCAAATTAGTCGATGGTCGT TTCCCCGATTATCGCCGTGTGTTACCTAAGGGCGGTGATAAAATCGTTATCGCCAGCCGTAACCACTTCAAGCAAGCCTT AACCCGTGCTTCTATTTTATCGAATGAGAAGTTTCGCGGTGTACGCATTCAGTTAGAAGCGGGATTACTGAAAATCACCG CCAATAACCCAGAGCAGGAAGAAGCGGAAGAAATCATCGATGTGGATTACAACAATCAGCCACTCGAAATTGGTTTCAAC GTCAGTTACTTATTGGATGTGCTAAACAACCTTAAGTCCGATGATGTACGTATCACCTTAATCGATGGTAACTCGAGTGC TTTATTGGAAAACCACCTGGAAGAAGATTCCATGTATGTGGTTATGCCGATGCGCTTATAG
Upstream 100 bases:
>100_bases GTAAGATTGCTCAGCTACGGGAAGAGAGTCACGACATTAAAGAAGATTATGCTAACTTGATTAGAACCTTATCTTCTTAA AATCAAGGAATTTGGACACT
Downstream 100 bases:
>100_bases TATTCCATTGCCTACTTTGTGCCATACGCCGCTTTTTAGCGGCGTATATGTCTTCAAGCCTTATCCTTTGTTGCCTTATT TACTCGGATGGATGTGAATT
Product: DNA polymerase III subunit beta
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 366; Mature: 366
Protein sequence:
>366_residues MKFSIDRDALLKPLQLVCGAVERRHNLPILANLLVEVSGHSLKMTGTDLEVELVGQAVIHGDIEEGRTTVPAKKLLDIVK SLPEQSELKVEQQDNKWLLRSGRSRFSLATLPAEEYPNVEAFQAEIEFTLKQGVLKSLIDATQFSMANQDVRYYLNGLLL ETEGNVLRAIATDGHRLALSHRTIDAQLPEKQVIVPRKGVMEMARLLEADDLDIAIAIGDNAIRATTSTTVFTSKLVDGR FPDYRRVLPKGGDKIVIASRNHFKQALTRASILSNEKFRGVRIQLEAGLLKITANNPEQEEAEEIIDVDYNNQPLEIGFN VSYLLDVLNNLKSDDVRITLIDGNSSALLENHLEEDSMYVVMPMRL
Sequences:
>Translated_366_residues MKFSIDRDALLKPLQLVCGAVERRHNLPILANLLVEVSGHSLKMTGTDLEVELVGQAVIHGDIEEGRTTVPAKKLLDIVK SLPEQSELKVEQQDNKWLLRSGRSRFSLATLPAEEYPNVEAFQAEIEFTLKQGVLKSLIDATQFSMANQDVRYYLNGLLL ETEGNVLRAIATDGHRLALSHRTIDAQLPEKQVIVPRKGVMEMARLLEADDLDIAIAIGDNAIRATTSTTVFTSKLVDGR FPDYRRVLPKGGDKIVIASRNHFKQALTRASILSNEKFRGVRIQLEAGLLKITANNPEQEEAEEIIDVDYNNQPLEIGFN VSYLLDVLNNLKSDDVRITLIDGNSSALLENHLEEDSMYVVMPMRL >Mature_366_residues MKFSIDRDALLKPLQLVCGAVERRHNLPILANLLVEVSGHSLKMTGTDLEVELVGQAVIHGDIEEGRTTVPAKKLLDIVK SLPEQSELKVEQQDNKWLLRSGRSRFSLATLPAEEYPNVEAFQAEIEFTLKQGVLKSLIDATQFSMANQDVRYYLNGLLL ETEGNVLRAIATDGHRLALSHRTIDAQLPEKQVIVPRKGVMEMARLLEADDLDIAIAIGDNAIRATTSTTVFTSKLVDGR FPDYRRVLPKGGDKIVIASRNHFKQALTRASILSNEKFRGVRIQLEAGLLKITANNPEQEEAEEIIDVDYNNQPLEIGFN VSYLLDVLNNLKSDDVRITLIDGNSSALLENHLEEDSMYVVMPMRL
Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped
COG id: COG0592
COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: NA
Homologues:
Organism=Escherichia coli, GI1790136, Length=366, Percent_Identity=57.3770491803279, Blast_Score=426, Evalue=1e-120,
Paralogues:
None
Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001001 - InterPro: IPR022635 - InterPro: IPR022637 - InterPro: IPR022634 [H]
Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]
EC number: =2.7.7.7 [H]
Molecular weight: Translated: 40876; Mature: 40876
Theoretical pI: Translated: 5.00; Mature: 5.00
Prosite motif: PS00639 THIOL_PROTEASE_HIS
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.3 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 2.5 %Cys+Met (Translated Protein) 0.3 %Cys (Mature Protein) 2.2 %Met (Mature Protein) 2.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKFSIDRDALLKPLQLVCGAVERRHNLPILANLLVEVSGHSLKMTGTDLEVELVGQAVIH CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECCCEEEEEECEEEEE GDIEEGRTTVPAKKLLDIVKSLPEQSELKVEQQDNKWLLRSGRSRFSLATLPAEEYPNVE CCHHCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCEEHHHCCCCCEEEEECCHHHCCCHH AFQAEIEFTLKQGVLKSLIDATQFSMANQDVRYYLNGLLLETEGNVLRAIATDGHRLALS EEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEECEEEEEECCCEEEEEECCCEEEEEE HRTIDAQLPEKQVIVPRKGVMEMARLLEADDLDIAIAIGDNAIRATTSTTVFTSKLVDGR ECEECCCCCCCEEECCCHHHHHHHHHHCCCCCEEEEEECCCCEEEECCHHEEEHHHHCCC FPDYRRVLPKGGDKIVIASRNHFKQALTRASILSNEKFRGVRIQLEAGLLKITANNPEQE CCHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEECCCCCHH EAEEIIDVDYNNQPLEIGFNVSYLLDVLNNLKSDDVRITLIDGNSSALLENHLEEDSMYV HHHHHEECCCCCCCEEECCCHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCCEEE VMPMRL EEECCC >Mature Secondary Structure MKFSIDRDALLKPLQLVCGAVERRHNLPILANLLVEVSGHSLKMTGTDLEVELVGQAVIH CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECCCEEEEEECEEEEE GDIEEGRTTVPAKKLLDIVKSLPEQSELKVEQQDNKWLLRSGRSRFSLATLPAEEYPNVE CCHHCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCEEHHHCCCCCEEEEECCHHHCCCHH AFQAEIEFTLKQGVLKSLIDATQFSMANQDVRYYLNGLLLETEGNVLRAIATDGHRLALS EEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEECEEEEEECCCEEEEEECCCEEEEEE HRTIDAQLPEKQVIVPRKGVMEMARLLEADDLDIAIAIGDNAIRATTSTTVFTSKLVDGR ECEECCCCCCCEEECCCHHHHHHHHHHCCCCCEEEEEECCCCEEEECCHHEEEHHHHCCC FPDYRRVLPKGGDKIVIASRNHFKQALTRASILSNEKFRGVRIQLEAGLLKITANNPEQE CCHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEECCCCCHH EAEEIIDVDYNNQPLEIGFNVSYLLDVLNNLKSDDVRITLIDGNSSALLENHLEEDSMYV HHHHHEECCCCCCCEEECCCHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCCEEE VMPMRL EEECCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796 [H]