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The map label for this gene is glgX [H]

Identifier: 113478340

GI number: 113478340

Start: 7666501

End: 7668621

Strand: Reverse

Name: glgX [H]

Synonym: Tery_5016

Alternate gene names: 113478340

Gene position: 7668621-7666501 (Counterclockwise)

Preceding gene: 113478341

Following gene: 113478339

Centisome position: 98.95

GC content: 39.98

Gene sequence:

>2121_bases
ATGTTACAAGAGCGTATTGATATCCATCCTACCCACAGTCATGGTGACTTTAAGCTCCGTAGTGGAAAACCTTTTCCATT
CGGTGCAACTATGGTGTCTGGAGGAGTAAACTTTTCCATCTTCTCTTCTTATGCTACATCTTGTACATTGGTTTTGTTTC
AAAAAGGTGCTTTAAAACCAATGGCAGAAATTCCTTTTCCTGAAGAATTTCGGATAGGGAATGTTTATTGTATGATTGTC
TTTGATCTTGATGTTGAGAACATCGAATATGGCTATCGCATGGATGGACCGAATAATTTCCATGAGGGTCAATGGTTCGA
CAAAAGTAAAATTCTCATGGACCCCTATGCTAAGATTATTGGGGGTAGGGATGTTTGGGGAAAAACACCAAACTGGGATG
ATATTTATCAACACCGGGGGAGAATTCCCTATGATGATTTTGACTGGGAAAATGACCGCCCACTAGAAATTCCACCACAA
GATCAGATTATCTATGAAATGCACGTTCGTAGCTTTACGGCTCATCCTTCTTCTGGTGTCAAACATCCAGGCACATTTGC
TGCTATTCGCGAAAAAATTCCTTACCTCAAAGAATTAGGTGTTAACGCGGTGGAGTTGATGCCTATCTATGAATTTGATG
AGTTTGAAAATTCTCGGCCCAGCCCCATCACAGGAGAAATGCTCTACAATTATTGGGGTTATAGTACAGTCGGGTTTTTT
GCTCCTAAGGCAGGGTACGCAGCGACAGGTAAGTTAGGAATGCAGATGGATGAGTTAAAAACTCTAGTTAAAGACCTGCA
CAAAAATGGGATTGAAGTAATTCTTGACGTTGTTTTCAACCACACAGCAGAAGGAAATGAAAACGGTCCGACAATTTCTT
TCCGGGGTATTGACAATAAGACATATTATATGCTAACACCAGAAGGTTATTATTTTAACTTTAGTGGTTGTGGCAATACT
ATTAATTGTAATAACCCCATTGTTCGTAATGTGGTGTTAGATTGTTTGCGCTATTGGGCAAGTGAATATCATATTGATGG
TTTCAGATTTGATTTGGCTTCAATTCTTGGACGTGACCCCTGGGGCGCACCTTTAGCAAATCCTCCATTATTAGAAACTT
TGGCTTTTGACCCAATTTTAGCTAACTGTAAGTTGATTGCAGAAGCTTGGGATGCTGGTGGTTTATATCAAGTGGGTTCC
TTCCCTGCTTTTGGACGTTGGGCCGAATGGAATGGTAAGTATCGCGATGCAATTCGCAAGTTTATTAAAGGTGATTGTAC
TGTAGGGGAAATGGCACAAAGGATACAGGGTTCTCCAGACCTCTATGCTTGGCAAGGTCGTGGACCGGCAACATCTATTA
ATTTTATTACTGCCCACGATGGTTTTACTTTGGCTGATTTGGTTTCCTACAATGATAAGCATAATGAAGCTAATGGTGAA
AATAATAATGATGGTGCTAATGATAACGAAAGTTGGAACTGTGGGGCAGAGGGTTGGAGTGATGACCCTGGAATAAATGC
TTTGCGTAGCCGTCAGATGCGAAATGCGATCGCAATTATGATGGTTAGTCAAGGTGTTCCTATGTTGCTGATGGGAGATG
AAATAGGGCGGAGTAAAAATGGTAATAATAATACTTATTGCCATGACTCAGAACTAAATTGGCTAAATTGGGAGCTTCTG
GAAACTAATGCTGAGTTATTTAGGTTTGTGAAGAATTGTGTGGCATTTCGGAAAGCTCACCCTATATTGAAAAATCAGAA
CCATTTCCGAAATCAAGATTATGTAGGTAGTGGAAAATCTGATATTACTTGGCATGGTACTCAAGCTTGGAATGCTGACT
GGTCAGATAATTCTCGAACTTTGGCTTTTATGCTTTGTGGTAAACATGCTACAGAGGTGACAAAGCAAGATAATGATATT
TATGTAGCAATGAATATGCACTGGGACTCTCTCTGGTTTAACATTCCCGGTTTATCAGATGGGAAACATTGGCATATTTT
TGTTAATACTGCTTGTGCTTCGCCAGAAGATGTTTGGGAACTTGGGAGCGAACCTGTTTTAGAAAATCAACATGGGTTAT
TACTTGGGGATCGATCTATAGTTATTTTAGTAAGTAAATAA

Upstream 100 bases:

>100_bases
GAAGAAACAAACGAACCCAAATAAACTAACACTATGCTAGTTGATTTAAACAACAAAACTTCTATCGTGCTCGTACTAAA
AAATATATTCACAAAGATTC

Downstream 100 bases:

>100_bases
AGTCGGAAATGATATTATAGAGCTTATGATCATATCTATGAATTGGGAGTAGGGAGGTTGAATAGAATATCTGTATAGAT
TAGAGGATATCAGGTGTATT

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 706; Mature: 706

Protein sequence:

>706_residues
MLQERIDIHPTHSHGDFKLRSGKPFPFGATMVSGGVNFSIFSSYATSCTLVLFQKGALKPMAEIPFPEEFRIGNVYCMIV
FDLDVENIEYGYRMDGPNNFHEGQWFDKSKILMDPYAKIIGGRDVWGKTPNWDDIYQHRGRIPYDDFDWENDRPLEIPPQ
DQIIYEMHVRSFTAHPSSGVKHPGTFAAIREKIPYLKELGVNAVELMPIYEFDEFENSRPSPITGEMLYNYWGYSTVGFF
APKAGYAATGKLGMQMDELKTLVKDLHKNGIEVILDVVFNHTAEGNENGPTISFRGIDNKTYYMLTPEGYYFNFSGCGNT
INCNNPIVRNVVLDCLRYWASEYHIDGFRFDLASILGRDPWGAPLANPPLLETLAFDPILANCKLIAEAWDAGGLYQVGS
FPAFGRWAEWNGKYRDAIRKFIKGDCTVGEMAQRIQGSPDLYAWQGRGPATSINFITAHDGFTLADLVSYNDKHNEANGE
NNNDGANDNESWNCGAEGWSDDPGINALRSRQMRNAIAIMMVSQGVPMLLMGDEIGRSKNGNNNTYCHDSELNWLNWELL
ETNAELFRFVKNCVAFRKAHPILKNQNHFRNQDYVGSGKSDITWHGTQAWNADWSDNSRTLAFMLCGKHATEVTKQDNDI
YVAMNMHWDSLWFNIPGLSDGKHWHIFVNTACASPEDVWELGSEPVLENQHGLLLGDRSIVILVSK

Sequences:

>Translated_706_residues
MLQERIDIHPTHSHGDFKLRSGKPFPFGATMVSGGVNFSIFSSYATSCTLVLFQKGALKPMAEIPFPEEFRIGNVYCMIV
FDLDVENIEYGYRMDGPNNFHEGQWFDKSKILMDPYAKIIGGRDVWGKTPNWDDIYQHRGRIPYDDFDWENDRPLEIPPQ
DQIIYEMHVRSFTAHPSSGVKHPGTFAAIREKIPYLKELGVNAVELMPIYEFDEFENSRPSPITGEMLYNYWGYSTVGFF
APKAGYAATGKLGMQMDELKTLVKDLHKNGIEVILDVVFNHTAEGNENGPTISFRGIDNKTYYMLTPEGYYFNFSGCGNT
INCNNPIVRNVVLDCLRYWASEYHIDGFRFDLASILGRDPWGAPLANPPLLETLAFDPILANCKLIAEAWDAGGLYQVGS
FPAFGRWAEWNGKYRDAIRKFIKGDCTVGEMAQRIQGSPDLYAWQGRGPATSINFITAHDGFTLADLVSYNDKHNEANGE
NNNDGANDNESWNCGAEGWSDDPGINALRSRQMRNAIAIMMVSQGVPMLLMGDEIGRSKNGNNNTYCHDSELNWLNWELL
ETNAELFRFVKNCVAFRKAHPILKNQNHFRNQDYVGSGKSDITWHGTQAWNADWSDNSRTLAFMLCGKHATEVTKQDNDI
YVAMNMHWDSLWFNIPGLSDGKHWHIFVNTACASPEDVWELGSEPVLENQHGLLLGDRSIVILVSK
>Mature_706_residues
MLQERIDIHPTHSHGDFKLRSGKPFPFGATMVSGGVNFSIFSSYATSCTLVLFQKGALKPMAEIPFPEEFRIGNVYCMIV
FDLDVENIEYGYRMDGPNNFHEGQWFDKSKILMDPYAKIIGGRDVWGKTPNWDDIYQHRGRIPYDDFDWENDRPLEIPPQ
DQIIYEMHVRSFTAHPSSGVKHPGTFAAIREKIPYLKELGVNAVELMPIYEFDEFENSRPSPITGEMLYNYWGYSTVGFF
APKAGYAATGKLGMQMDELKTLVKDLHKNGIEVILDVVFNHTAEGNENGPTISFRGIDNKTYYMLTPEGYYFNFSGCGNT
INCNNPIVRNVVLDCLRYWASEYHIDGFRFDLASILGRDPWGAPLANPPLLETLAFDPILANCKLIAEAWDAGGLYQVGS
FPAFGRWAEWNGKYRDAIRKFIKGDCTVGEMAQRIQGSPDLYAWQGRGPATSINFITAHDGFTLADLVSYNDKHNEANGE
NNNDGANDNESWNCGAEGWSDDPGINALRSRQMRNAIAIMMVSQGVPMLLMGDEIGRSKNGNNNTYCHDSELNWLNWELL
ETNAELFRFVKNCVAFRKAHPILKNQNHFRNQDYVGSGKSDITWHGTQAWNADWSDNSRTLAFMLCGKHATEVTKQDNDI
YVAMNMHWDSLWFNIPGLSDGKHWHIFVNTACASPEDVWELGSEPVLENQHGLLLGDRSIVILVSK

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=214, Percent_Identity=28.5046728971963, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI2367229, Length=580, Percent_Identity=40.5172413793103, Blast_Score=401, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6320826, Length=225, Percent_Identity=28.4444444444444, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 79868; Mature: 79868

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQERIDIHPTHSHGDFKLRSGKPFPFGATMVSGGVNFSIFSSYATSCTLVLFQKGALKP
CCCCCCCCCCCCCCCCEEECCCCCCCCCHHEECCCCCEEEHHHHHCEEEEEEEECCCCCC
MAEIPFPEEFRIGNVYCMIVFDLDVENIEYGYRMDGPNNFHEGQWFDKSKILMDPYAKII
HHHCCCCCCEEECCEEEEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCEECCCHHHHH
GGRDVWGKTPNWDDIYQHRGRIPYDDFDWENDRPLEIPPQDQIIYEMHVRSFTAHPSSGV
CCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHEECCCCCCC
KHPGTFAAIREKIPYLKELGVNAVELMPIYEFDEFENSRPSPITGEMLYNYWGYSTVGFF
CCCCHHHHHHHHCCHHHHHCCCEEEEEEEEECHHHCCCCCCCCCHHHHHHHCCCCCCEEE
APKAGYAATGKLGMQMDELKTLVKDLHKNGIEVILDVVFNHTAEGNENGPTISFRGIDNK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCC
TYYMLTPEGYYFNFSGCGNTINCNNPIVRNVVLDCLRYWASEYHIDGFRFDLASILGRDP
EEEEECCCCEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
WGAPLANPPLLETLAFDPILANCKLIAEAWDAGGLYQVGSFPAFGRWAEWNGKYRDAIRK
CCCCCCCCCHHHHHHCCCHHHCCEEEEECCCCCCEEECCCCCCCCCCCCCCCHHHHHHHH
FIKGDCTVGEMAQRIQGSPDLYAWQGRGPATSINFITAHDGFTLADLVSYNDKHNEANGE
HHCCCCCHHHHHHHHCCCCCEEEECCCCCCCEEEEEEECCCCCHHHHHHCCCCCCCCCCC
NNNDGANDNESWNCGAEGWSDDPGINALRSRQMRNAIAIMMVSQGVPMLLMGDEIGRSKN
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEEECCCCCEEEECCHHCCCCC
GNNNTYCHDSELNWLNWELLETNAELFRFVKNCVAFRKAHPILKNQNHFRNQDYVGSGKS
CCCCCEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
DITWHGTQAWNADWSDNSRTLAFMLCGKHATEVTKQDNDIYVAMNMHWDSLWFNIPGLSD
CEEECCCCCCCCCCCCCCCEEEEEEECCCHHHHHCCCCCEEEEEEEEECEEEEECCCCCC
GKHWHIFVNTACASPEDVWELGSEPVLENQHGLLLGDRSIVILVSK
CCEEEEEEEECCCCHHHHHHCCCCCCCCCCCCEEEECCEEEEEEEC
>Mature Secondary Structure
MLQERIDIHPTHSHGDFKLRSGKPFPFGATMVSGGVNFSIFSSYATSCTLVLFQKGALKP
CCCCCCCCCCCCCCCCEEECCCCCCCCCHHEECCCCCEEEHHHHHCEEEEEEEECCCCCC
MAEIPFPEEFRIGNVYCMIVFDLDVENIEYGYRMDGPNNFHEGQWFDKSKILMDPYAKII
HHHCCCCCCEEECCEEEEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCEECCCHHHHH
GGRDVWGKTPNWDDIYQHRGRIPYDDFDWENDRPLEIPPQDQIIYEMHVRSFTAHPSSGV
CCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHEECCCCCCC
KHPGTFAAIREKIPYLKELGVNAVELMPIYEFDEFENSRPSPITGEMLYNYWGYSTVGFF
CCCCHHHHHHHHCCHHHHHCCCEEEEEEEEECHHHCCCCCCCCCHHHHHHHCCCCCCEEE
APKAGYAATGKLGMQMDELKTLVKDLHKNGIEVILDVVFNHTAEGNENGPTISFRGIDNK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCC
TYYMLTPEGYYFNFSGCGNTINCNNPIVRNVVLDCLRYWASEYHIDGFRFDLASILGRDP
EEEEECCCCEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
WGAPLANPPLLETLAFDPILANCKLIAEAWDAGGLYQVGSFPAFGRWAEWNGKYRDAIRK
CCCCCCCCCHHHHHHCCCHHHCCEEEEECCCCCCEEECCCCCCCCCCCCCCCHHHHHHHH
FIKGDCTVGEMAQRIQGSPDLYAWQGRGPATSINFITAHDGFTLADLVSYNDKHNEANGE
HHCCCCCHHHHHHHHCCCCCEEEECCCCCCCEEEEEEECCCCCHHHHHHCCCCCCCCCCC
NNNDGANDNESWNCGAEGWSDDPGINALRSRQMRNAIAIMMVSQGVPMLLMGDEIGRSKN
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEEECCCCCEEEECCHHCCCCC
GNNNTYCHDSELNWLNWELLETNAELFRFVKNCVAFRKAHPILKNQNHFRNQDYVGSGKS
CCCCCEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
DITWHGTQAWNADWSDNSRTLAFMLCGKHATEVTKQDNDIYVAMNMHWDSLWFNIPGLSD
CEEECCCCCCCCCCCCCCCEEEEEEECCCHHHHHCCCCCEEEEEEEEECEEEEECCCCCC
GKHWHIFVNTACASPEDVWELGSEPVLENQHGLLLGDRSIVILVSK
CCEEEEEEEECCCCHHHHHHCCCCCCCCCCCCEEEECCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]