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The map label for this gene is pntA [H]

Identifier: 113478080

GI number: 113478080

Start: 7228305

End: 7229447

Strand: Reverse

Name: pntA [H]

Synonym: Tery_4705

Alternate gene names: 113478080

Gene position: 7229447-7228305 (Counterclockwise)

Preceding gene: 113478090

Following gene: 113478079

Centisome position: 93.28

GC content: 39.98

Gene sequence:

>1143_bases
ATGAAAATATCGGTAGCTAAGGAAATTTTGGTCAATGAATGTCGTGTGGCCTTAGTTCCTGATGTTATAGCCAAATTAGT
TAAACAAGGGCTAGAAATACAAGTAGAAGCAGGTGCAGGAGAAAAATCTTTTTTCTTTGATAGAGCTTATGAACAAGCTG
GAGCAAAAATCATTTCTGATAGTAATGCTCTGTGGGGTAAAACTAAGGTGCTCCTAAAAATTGGAGTACTAGAGGAATCA
GAAGTGGACAAATTACGGGAGGGCAGCATTGTTATTAGTTTCCTCAACCCCCTAGGAAACCCAACTATTGTAAAGCGTTT
AGCTGAAAGGAAAGTAACTGCATTTAGTATGGAGCTAATTCCTCGTACTAGTAGAGCACAAGTTATGGATGCTTTATCTT
CCCAAGCTAATATTGGTGGATATAAGGCGGTTTTGCTGGCGTCTGCTGCTTTACCTAAGTTTTTCCCAATGTTAACTACC
GCAGCAGGTACTATTAAACCTGCTAAAGTATTAGTGATGGGGGCAGGAGTTGCTGGTTTACAGGCGATCGCTACTGCTCG
TCGTCTTGGTGCTGTAGTAGAAGCATTTGATATCCGTCCAGAAGTTAAAGAACAAGTACAAAGTTTGGGAGCAAAATTTG
TAGATGTGAGCCTCAAAGAAGATACTGTTGCTGAGGGAGGATACGCAAAAGAACTTTCCGAGCAAGCTAAACAGCATACT
CGTGAAGTTCTGACACAGCATGTTGCTGTTTCAGACATTGTCATTACCACTGCTCAAGTTCCTGGTAAAAAAGCACCATT
GCTAGTAACTAAGGAAATGGTAGAGCAAATGAAACCAGGAACAGTAATAGTTGATATGGCAGCTGGACAAGGAGGTAATT
GTGAATGTACTCAACCAGGTAAAGATGTACAGTGGAATGGCGTGACTATTATTGGTCCTTTAAATTTACCAGCAACTATG
CCAGTTCACGCTAGTGAATTATATGCTAAAAATATTTCTGCTTTACTCAAGTTGATGATTAAAGATGGAGAACTCAATCT
TGACTTTGAAGATGATATCATTGCTGGTGCTTGTGTTACCCATGGAGGTGAAATTCGCAATCAAAAAGTCAAAGATGCTC
TAAGAGTTACTATTGGTGGTTGA

Upstream 100 bases:

>100_bases
TTAAAGATAGCAATGAGCAACGAAATATAAGCCTGATAATTTTAGATAATAAGCAAATAGTAAAAAACTATAATCTAAAG
ACTGCTTTAAGGGAGAATTT

Downstream 100 bases:

>100_bases
TCAAAAAAGTATTAAGTGATCGGCATTCAGCAGGAGGGTTTTTTATTGTTAAGAAGGAGATTTTCACAAAGGTAGACTAC
AAAAAACTTCTGCACTCTAC

Product: NAD(P)(+) transhydrogenase

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MKISVAKEILVNECRVALVPDVIAKLVKQGLEIQVEAGAGEKSFFFDRAYEQAGAKIISDSNALWGKTKVLLKIGVLEES
EVDKLREGSIVISFLNPLGNPTIVKRLAERKVTAFSMELIPRTSRAQVMDALSSQANIGGYKAVLLASAALPKFFPMLTT
AAGTIKPAKVLVMGAGVAGLQAIATARRLGAVVEAFDIRPEVKEQVQSLGAKFVDVSLKEDTVAEGGYAKELSEQAKQHT
REVLTQHVAVSDIVITTAQVPGKKAPLLVTKEMVEQMKPGTVIVDMAAGQGGNCECTQPGKDVQWNGVTIIGPLNLPATM
PVHASELYAKNISALLKLMIKDGELNLDFEDDIIAGACVTHGGEIRNQKVKDALRVTIGG

Sequences:

>Translated_380_residues
MKISVAKEILVNECRVALVPDVIAKLVKQGLEIQVEAGAGEKSFFFDRAYEQAGAKIISDSNALWGKTKVLLKIGVLEES
EVDKLREGSIVISFLNPLGNPTIVKRLAERKVTAFSMELIPRTSRAQVMDALSSQANIGGYKAVLLASAALPKFFPMLTT
AAGTIKPAKVLVMGAGVAGLQAIATARRLGAVVEAFDIRPEVKEQVQSLGAKFVDVSLKEDTVAEGGYAKELSEQAKQHT
REVLTQHVAVSDIVITTAQVPGKKAPLLVTKEMVEQMKPGTVIVDMAAGQGGNCECTQPGKDVQWNGVTIIGPLNLPATM
PVHASELYAKNISALLKLMIKDGELNLDFEDDIIAGACVTHGGEIRNQKVKDALRVTIGG
>Mature_380_residues
MKISVAKEILVNECRVALVPDVIAKLVKQGLEIQVEAGAGEKSFFFDRAYEQAGAKIISDSNALWGKTKVLLKIGVLEES
EVDKLREGSIVISFLNPLGNPTIVKRLAERKVTAFSMELIPRTSRAQVMDALSSQANIGGYKAVLLASAALPKFFPMLTT
AAGTIKPAKVLVMGAGVAGLQAIATARRLGAVVEAFDIRPEVKEQVQSLGAKFVDVSLKEDTVAEGGYAKELSEQAKQHT
REVLTQHVAVSDIVITTAQVPGKKAPLLVTKEMVEQMKPGTVIVDMAAGQGGNCECTQPGKDVQWNGVTIIGPLNLPATM
PVHASELYAKNISALLKLMIKDGELNLDFEDDIIAGACVTHGGEIRNQKVKDALRVTIGG

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=375, Percent_Identity=43.4666666666667, Blast_Score=284, Evalue=1e-76,
Organism=Homo sapiens, GI122939153, Length=375, Percent_Identity=43.4666666666667, Blast_Score=284, Evalue=1e-76,
Organism=Escherichia coli, GI1787887, Length=368, Percent_Identity=46.7391304347826, Blast_Score=333, Evalue=9e-93,
Organism=Caenorhabditis elegans, GI17550456, Length=366, Percent_Identity=43.9890710382514, Blast_Score=303, Evalue=8e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR016040
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 40534; Mature: 40534

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISVAKEILVNECRVALVPDVIAKLVKQGLEIQVEAGAGEKSFFFDRAYEQAGAKIISD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCEEECC
SNALWGKTKVLLKIGVLEESEVDKLREGSIVISFLNPLGNPTIVKRLAERKVTAFSMELI
CCCCCCCEEEEEEEECCCCHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHEEHHHC
PRTSRAQVMDALSSQANIGGYKAVLLASAALPKFFPMLTTAAGTIKPAKVLVMGAGVAGL
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHH
QAIATARRLGAVVEAFDIRPEVKEQVQSLGAKFVDVSLKEDTVAEGGYAKELSEQAKQHT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHH
REVLTQHVAVSDIVITTAQVPGKKAPLLVTKEMVEQMKPGTVIVDMAAGQGGNCECTQPG
HHHHHHHHHHHHEEEEEECCCCCCCCEEEEHHHHHHCCCCEEEEEECCCCCCCEEECCCC
KDVQWNGVTIIGPLNLPATMPVHASELYAKNISALLKLMIKDGELNLDFEDDIIAGACVT
CCEEECCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHH
HGGEIRNQKVKDALRVTIGG
CCCHHHHHHHHHHHEEEECC
>Mature Secondary Structure
MKISVAKEILVNECRVALVPDVIAKLVKQGLEIQVEAGAGEKSFFFDRAYEQAGAKIISD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCEEECC
SNALWGKTKVLLKIGVLEESEVDKLREGSIVISFLNPLGNPTIVKRLAERKVTAFSMELI
CCCCCCCEEEEEEEECCCCHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHEEHHHC
PRTSRAQVMDALSSQANIGGYKAVLLASAALPKFFPMLTTAAGTIKPAKVLVMGAGVAGL
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHH
QAIATARRLGAVVEAFDIRPEVKEQVQSLGAKFVDVSLKEDTVAEGGYAKELSEQAKQHT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHH
REVLTQHVAVSDIVITTAQVPGKKAPLLVTKEMVEQMKPGTVIVDMAAGQGGNCECTQPG
HHHHHHHHHHHHEEEEEECCCCCCCCEEEEHHHHHHCCCCEEEEEECCCCCCCEEECCCC
KDVQWNGVTIIGPLNLPATMPVHASELYAKNISALLKLMIKDGELNLDFEDDIIAGACVT
CCEEECCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHH
HGGEIRNQKVKDALRVTIGG
CCCHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]