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The map label for this gene is ispG

Identifier: 113477916

GI number: 113477916

Start: 6977513

End: 6978733

Strand: Reverse

Name: ispG

Synonym: Tery_4522

Alternate gene names: 113477916

Gene position: 6978733-6977513 (Counterclockwise)

Preceding gene: 113477917

Following gene: 113477915

Centisome position: 90.05

GC content: 41.77

Gene sequence:

>1221_bases
ATGCAAACCCTAGCAAACCCAAGCAAAACCCCAATAAACCAAGCTGTATTTGACACTACTATCCATCGGCGAAAAACTCG
TTCTGTCAAAGTGGGTGATATAACCATCGGTGGTGGCCATCCAGTTGTAGTTCAGTCAATGATCAACGAAGATACTCTTG
ACATTGAGAGATCCGTAGCTGCTATTCGGCGTTTACATGAAATAGGTTGCGAAATTGTTCGTGTCACTGTCCCTAGTATA
GCCCATGCTAAATCTCTAGGAGAAATCAAGCAAAAATTAGCAGAAACCTACAAACCTGTCCCACTGGTTGCCGACGTTCA
TCATAACGGCATGAAAATTGCTTTAGAAGTGGCCAAACAGGTTGATAAAGTGCGAATTAATCCTGGTTTATATGTATTTG
AAAAGCCACAAGCTGAGAGAAACGAATATACTCAGGCAGAATTTGAAGAAATAGGTGATAAAATTCGCCAAACTCTAAAG
CCCTTAGTAGTCTCTCTCAGAGACCAAAACAAAGCTATGCGCATTGGAGTTAATCATGGCTCCCTTTCTGAAAGGATGCT
GTTTACTTATGGGGACACCCCAGAAGGTATGGTAGAGTCTGCCTTAGAATTTATCAAAATTTGTGAATCTTTAAAATACT
ACAATTTGGTAATTTCTCTGAAAGCTTCACGGGTGCCGGTAATGCTAGCTGCTTATCGATTAATGGTGAAGCGGATGGAT
GAACTAGGTATGCCCTACCCCCTACATTTGGGTGTGACAGAAGCGGGGGATGGAGATTATGGTCGAGTTAAGTCTACTGC
TGGTATTGGCACACTGTTAGCAGAAGGAATAGGGGATACAATTCGCGTATCTTTAACTGAAGCACCCGAAAAAGAGATTC
CAGTTTGTTACAGTATTTTGCAAGCCTTGGGTCTGCGAAAAACAATGGTAGAATATGTAGCTTGTCCCTCCTGTGGCCGA
ACCTTATTTAATCTGGAAAATGTTCTTCATGAAGTGCGGGAAGCAACAAAACATCTAACAGGATTGGATATTGCTGTCAT
GGGTTGTATTGTTAATGGTCCAGGAGAAATGGCTGATGCAGATTATGGTTATGTAGGCAAGCAATCTGGCTATATCTCGT
TATATCGTGGGCGGGAAGAAATTAAGAAAGTCCACGAATCTCAAGGAGTTGAAGAATTGATTAATTTAATTAAAGCTGAT
GGGCGTTGGGTTGAACCGTAG

Upstream 100 bases:

>100_bases
AATTATAAAGATCAAATTCAGCAGTTATATGTCAACCTGAAATATAGGAAAATAATAAACTACTAAACCGTTCGCTAGAG
AAAAACAAAGATATTATATT

Downstream 100 bases:

>100_bases
TTTCTAATCAATCTGTTTGTGCTAGGAGTGTATTTTTACTACACTCCTAAACTAATTATCTGATGCTCTTGCCAAAATAA
AATTAATTGTTGATGAATGT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MQTLANPSKTPINQAVFDTTIHRRKTRSVKVGDITIGGGHPVVVQSMINEDTLDIERSVAAIRRLHEIGCEIVRVTVPSI
AHAKSLGEIKQKLAETYKPVPLVADVHHNGMKIALEVAKQVDKVRINPGLYVFEKPQAERNEYTQAEFEEIGDKIRQTLK
PLVVSLRDQNKAMRIGVNHGSLSERMLFTYGDTPEGMVESALEFIKICESLKYYNLVISLKASRVPVMLAAYRLMVKRMD
ELGMPYPLHLGVTEAGDGDYGRVKSTAGIGTLLAEGIGDTIRVSLTEAPEKEIPVCYSILQALGLRKTMVEYVACPSCGR
TLFNLENVLHEVREATKHLTGLDIAVMGCIVNGPGEMADADYGYVGKQSGYISLYRGREEIKKVHESQGVEELINLIKAD
GRWVEP

Sequences:

>Translated_406_residues
MQTLANPSKTPINQAVFDTTIHRRKTRSVKVGDITIGGGHPVVVQSMINEDTLDIERSVAAIRRLHEIGCEIVRVTVPSI
AHAKSLGEIKQKLAETYKPVPLVADVHHNGMKIALEVAKQVDKVRINPGLYVFEKPQAERNEYTQAEFEEIGDKIRQTLK
PLVVSLRDQNKAMRIGVNHGSLSERMLFTYGDTPEGMVESALEFIKICESLKYYNLVISLKASRVPVMLAAYRLMVKRMD
ELGMPYPLHLGVTEAGDGDYGRVKSTAGIGTLLAEGIGDTIRVSLTEAPEKEIPVCYSILQALGLRKTMVEYVACPSCGR
TLFNLENVLHEVREATKHLTGLDIAVMGCIVNGPGEMADADYGYVGKQSGYISLYRGREEIKKVHESQGVEELINLIKAD
GRWVEP
>Mature_406_residues
MQTLANPSKTPINQAVFDTTIHRRKTRSVKVGDITIGGGHPVVVQSMINEDTLDIERSVAAIRRLHEIGCEIVRVTVPSI
AHAKSLGEIKQKLAETYKPVPLVADVHHNGMKIALEVAKQVDKVRINPGLYVFEKPQAERNEYTQAEFEEIGDKIRQTLK
PLVVSLRDQNKAMRIGVNHGSLSERMLFTYGDTPEGMVESALEFIKICESLKYYNLVISLKASRVPVMLAAYRLMVKRMD
ELGMPYPLHLGVTEAGDGDYGRVKSTAGIGTLLAEGIGDTIRVSLTEAPEKEIPVCYSILQALGLRKTMVEYVACPSCGR
TLFNLENVLHEVREATKHLTGLDIAVMGCIVNGPGEMADADYGYVGKQSGYISLYRGREEIKKVHESQGVEELINLIKAD
GRWVEP

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=355, Percent_Identity=39.4366197183099, Blast_Score=237, Evalue=8e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_TRIEI (Q10W70)

Other databases:

- EMBL:   CP000393
- RefSeq:   YP_723977.1
- ProteinModelPortal:   Q10W70
- STRING:   Q10W70
- GeneID:   4246176
- GenomeReviews:   CP000393_GR
- KEGG:   ter:Tery_4522
- NMPDR:   fig|203124.1.peg.3281
- eggNOG:   COG0821
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- PhylomeDB:   Q10W70
- ProtClustDB:   PRK00366
- BioCyc:   TERY203124:TERY_4522-MONOMER
- HAMAP:   MF_00159
- InterPro:   IPR016425
- InterPro:   IPR004588
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE

EC number: =1.17.7.1

Molecular weight: Translated: 44925; Mature: 44925

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTLANPSKTPINQAVFDTTIHRRKTRSVKVGDITIGGGHPVVVQSMINEDTLDIERSVA
CCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHCCCHHHHHHHHH
AIRRLHEIGCEIVRVTVPSIAHAKSLGEIKQKLAETYKPVPLVADVHHNGMKIALEVAKQ
HHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHH
VDKVRINPGLYVFEKPQAERNEYTQAEFEEIGDKIRQTLKPLVVSLRDQNKAMRIGVNHG
HHHEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
SLSERMLFTYGDTPEGMVESALEFIKICESLKYYNLVISLKASRVPVMLAAYRLMVKRMD
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHH
ELGMPYPLHLGVTEAGDGDYGRVKSTAGIGTLLAEGIGDTIRVSLTEAPEKEIPVCYSIL
HCCCCCEEEECCEECCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHH
QALGLRKTMVEYVACPSCGRTLFNLENVLHEVREATKHLTGLDIAVMGCIVNGPGEMADA
HHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
DYGYVGKQSGYISLYRGREEIKKVHESQGVEELINLIKADGRWVEP
CCCCCCCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQTLANPSKTPINQAVFDTTIHRRKTRSVKVGDITIGGGHPVVVQSMINEDTLDIERSVA
CCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHCCCHHHHHHHHH
AIRRLHEIGCEIVRVTVPSIAHAKSLGEIKQKLAETYKPVPLVADVHHNGMKIALEVAKQ
HHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHH
VDKVRINPGLYVFEKPQAERNEYTQAEFEEIGDKIRQTLKPLVVSLRDQNKAMRIGVNHG
HHHEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
SLSERMLFTYGDTPEGMVESALEFIKICESLKYYNLVISLKASRVPVMLAAYRLMVKRMD
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHH
ELGMPYPLHLGVTEAGDGDYGRVKSTAGIGTLLAEGIGDTIRVSLTEAPEKEIPVCYSIL
HCCCCCEEEECCEECCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHH
QALGLRKTMVEYVACPSCGRTLFNLENVLHEVREATKHLTGLDIAVMGCIVNGPGEMADA
HHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
DYGYVGKQSGYISLYRGREEIKKVHESQGVEELINLIKADGRWVEP
CCCCCCCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA