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The map label for this gene is cobA [H]

Identifier: 113476840

GI number: 113476840

Start: 5100035

End: 5101573

Strand: Reverse

Name: cobA [H]

Synonym: Tery_3325

Alternate gene names: 113476840

Gene position: 5101573-5100035 (Counterclockwise)

Preceding gene: 113476853

Following gene: 113476831

Centisome position: 65.83

GC content: 32.88

Gene sequence:

>1539_bases
ATGAAAGTATATATCATTGGAGCCGGACCCGGAAATAAAGAATATTTAACCCTCAAAGCACATGCCCTAATTACTCAAGG
GGAAGTATTAATTTATGATGCCTTAATTGATGACTCTATTTTAGAACTAGCACCAGAAAATTGTTTAAAAATAGAGATGG
GTAAACGTGGCGGTAAACCCAGTATATCTCAGTCAGAAATTAATAGCTTATTAATAGAAAAATATAATTTAGGCAAACAA
GTAATTAGACTAAAAGGAGGAGACCCCTTTATTTTTGGTCGTGCTACTCCAGAAATTCAAGCATTAATTAAAGCAGGTTG
TGAATTTGAAATAGTACCAGGAATTTCCTCTACATTAGCTGCACCTTTACTAGCAAATATTCCTTTAACAGATACAGTAA
TGAGTAGAGCTTTTGTCATCTTAACTGCTCATGACATAAAAGTATTAGACTGGGAAACAATATCTAAAATTGAAACTTTA
GTAATTTTAATGGGGGCAAAAAATTTAGGTGAAATTGTACATCAGTTATTAAAACACCAAAGAAAACCTCAAACTCCTGT
AGCTATTATCAAAAATTGTGCAACTCCTAAACAACAAATTTGGATTAGTAATTTGAGCAATATTGCTAAACAAACTTATG
CTGAATATTTATCCCCTTGTGTGATAATAGTAGGAGAAGTAGTCAGATTAAGAGATTATTTACAATATCCAAATAACATG
GTTAGAGAAAAGTCAGAAGCCAAAACAGAGATAGCACCTTTAGCAAATAAAACTATTTTAGTAACTCGTTCAGCAGGTCA
GTCAAGTAAGTTTAGCGAACTTCTCAAAAAACAAGGAGCAAAAGTAATTGAAATGCCAGCTTTAGTTATTACTCCCCCTT
CAAGTTGGCATGATTTAGACCTAGGTATTACTAATATAAATAGTTTTGATTGGTTAATTCTTACTTCTACAAATGGAGTA
GACTATTTCTTTGAAAGATTAGCAGAGAAAGGTAAAGATAGTCGTGCTTTAGCTGGAGTAAAAATAGCAGTAGTTGGTAA
AAAAACTGCAGAAAGTTTGAAGACTTGTGGTTTAGTACCAGATTTTATTCCACCTAACTTTGTTGCTGATTCTTTAGTAG
CGAATTTTCCCGAAAAATTGAGTGGGAAAAAGATTTTATTTCCCAGAGTAGAAACTGGTGGTCGAGAAGTATTAGTTAAG
GAATTAAATAGTCAAGGTGCAGAGGTAATAGAAGTAGCTGCTTATGAGTCTAGTTGCCCAGAAAATATTTTACCCGAAGC
TTTGTTAGCCCTACAGAATTATCACATAGATGTTATTACTTTTGCTAGTTCTAAAACTGTCAAAAACTTTTGTTATTTAA
TTGGGGAAAATAAGGATAAATTACCCGAAGATTTATTAGAAAATATTTGTATTGCTTCTATCGGACCTCAAACTTCTCAA
AGTTGTCATAGTCTATTAGGTAGAGTAGATGTGGAAGCTCAAGAATATACTTTGGATGGATTGACTAAAGCTATTATTGA
GTGGGTAAGAGTAGCTTAA

Upstream 100 bases:

>100_bases
CAGCCTCCTAACCTAAACCCATAGTTATAAAACCAATGTTTAGCTTTACTGCGTTATATCCAACCTACTTTTTACCGATA
ACTAATAACTAATAACTGAA

Downstream 100 bases:

>100_bases
AGATTAAAAGTCAAAGTTCAAAATTTCAAAGTAAGATAAATATAGTAATTCCCATACTATTGAGCTACAAAATTATTTGG
TGGCGCCCGAATAGTTAATA

Product: uroporphyrinogen-III synthase / uroporphyrinogen-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MKVYIIGAGPGNKEYLTLKAHALITQGEVLIYDALIDDSILELAPENCLKIEMGKRGGKPSISQSEINSLLIEKYNLGKQ
VIRLKGGDPFIFGRATPEIQALIKAGCEFEIVPGISSTLAAPLLANIPLTDTVMSRAFVILTAHDIKVLDWETISKIETL
VILMGAKNLGEIVHQLLKHQRKPQTPVAIIKNCATPKQQIWISNLSNIAKQTYAEYLSPCVIIVGEVVRLRDYLQYPNNM
VREKSEAKTEIAPLANKTILVTRSAGQSSKFSELLKKQGAKVIEMPALVITPPSSWHDLDLGITNINSFDWLILTSTNGV
DYFFERLAEKGKDSRALAGVKIAVVGKKTAESLKTCGLVPDFIPPNFVADSLVANFPEKLSGKKILFPRVETGGREVLVK
ELNSQGAEVIEVAAYESSCPENILPEALLALQNYHIDVITFASSKTVKNFCYLIGENKDKLPEDLLENICIASIGPQTSQ
SCHSLLGRVDVEAQEYTLDGLTKAIIEWVRVA

Sequences:

>Translated_512_residues
MKVYIIGAGPGNKEYLTLKAHALITQGEVLIYDALIDDSILELAPENCLKIEMGKRGGKPSISQSEINSLLIEKYNLGKQ
VIRLKGGDPFIFGRATPEIQALIKAGCEFEIVPGISSTLAAPLLANIPLTDTVMSRAFVILTAHDIKVLDWETISKIETL
VILMGAKNLGEIVHQLLKHQRKPQTPVAIIKNCATPKQQIWISNLSNIAKQTYAEYLSPCVIIVGEVVRLRDYLQYPNNM
VREKSEAKTEIAPLANKTILVTRSAGQSSKFSELLKKQGAKVIEMPALVITPPSSWHDLDLGITNINSFDWLILTSTNGV
DYFFERLAEKGKDSRALAGVKIAVVGKKTAESLKTCGLVPDFIPPNFVADSLVANFPEKLSGKKILFPRVETGGREVLVK
ELNSQGAEVIEVAAYESSCPENILPEALLALQNYHIDVITFASSKTVKNFCYLIGENKDKLPEDLLENICIASIGPQTSQ
SCHSLLGRVDVEAQEYTLDGLTKAIIEWVRVA
>Mature_512_residues
MKVYIIGAGPGNKEYLTLKAHALITQGEVLIYDALIDDSILELAPENCLKIEMGKRGGKPSISQSEINSLLIEKYNLGKQ
VIRLKGGDPFIFGRATPEIQALIKAGCEFEIVPGISSTLAAPLLANIPLTDTVMSRAFVILTAHDIKVLDWETISKIETL
VILMGAKNLGEIVHQLLKHQRKPQTPVAIIKNCATPKQQIWISNLSNIAKQTYAEYLSPCVIIVGEVVRLRDYLQYPNNM
VREKSEAKTEIAPLANKTILVTRSAGQSSKFSELLKKQGAKVIEMPALVITPPSSWHDLDLGITNINSFDWLILTSTNGV
DYFFERLAEKGKDSRALAGVKIAVVGKKTAESLKTCGLVPDFIPPNFVADSLVANFPEKLSGKKILFPRVETGGREVLVK
ELNSQGAEVIEVAAYESSCPENILPEALLALQNYHIDVITFASSKTVKNFCYLIGENKDKLPEDLLENICIASIGPQTSQ
SCHSLLGRVDVEAQEYTLDGLTKAIIEWVRVA

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG1587

COG function: function code H; Uroporphyrinogen-III synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=245, Percent_Identity=37.5510204081633, Blast_Score=166, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6322922, Length=231, Percent_Identity=30.3030303030303, Blast_Score=94, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 56161; Mature: 56161

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVYIIGAGPGNKEYLTLKAHALITQGEVLIYDALIDDSILELAPENCLKIEMGKRGGKP
CEEEEEECCCCCCCEEEEEEEEEEECCCEEEEEEHHCCHHHHHCCCCCEEEEECCCCCCC
SISQSEINSLLIEKYNLGKQVIRLKGGDPFIFGRATPEIQALIKAGCEFEIVPGISSTLA
CCCHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCHHHHHHHHCCCCEEEECCCHHHHH
APLLANIPLTDTVMSRAFVILTAHDIKVLDWETISKIETLVILMGAKNLGEIVHQLLKHQ
HHHHCCCCCHHHHHHCEEEEEEECCEEEECHHHHHHHHHEEEEECCCHHHHHHHHHHHHC
RKPQTPVAIIKNCATPKQQIWISNLSNIAKQTYAEYLSPCVIIVGEVVRLRDYLQYPNNM
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VREKSEAKTEIAPLANKTILVTRSAGQSSKFSELLKKQGAKVIEMPALVITPPSSWHDLD
HHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCEEECCEEEEECCCCCCEEE
LGITNINSFDWLILTSTNGVDYFFERLAEKGKDSRALAGVKIAVVGKKTAESLKTCGLVP
CEEECCCCEEEEEEECCCCHHHHHHHHHHCCCCCCEECCEEEEEECCHHHHHHHHCCCCC
DFIPPNFVADSLVANFPEKLSGKKILFPRVETGGREVLVKELNSQGAEVIEVAAYESSCP
CCCCCHHHHHHHHHCCHHHHCCCEEEECCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCC
ENILPEALLALQNYHIDVITFASSKTVKNFCYLIGENKDKLPEDLLENICIASIGPQTSQ
HHHHHHHHHHHHCCCEEEEEECCCHHHHHHHEEECCCCCCCHHHHHHHHHEECCCCCCHH
SCHSLLGRVDVEAQEYTLDGLTKAIIEWVRVA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVYIIGAGPGNKEYLTLKAHALITQGEVLIYDALIDDSILELAPENCLKIEMGKRGGKP
CEEEEEECCCCCCCEEEEEEEEEEECCCEEEEEEHHCCHHHHHCCCCCEEEEECCCCCCC
SISQSEINSLLIEKYNLGKQVIRLKGGDPFIFGRATPEIQALIKAGCEFEIVPGISSTLA
CCCHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCHHHHHHHHCCCCEEEECCCHHHHH
APLLANIPLTDTVMSRAFVILTAHDIKVLDWETISKIETLVILMGAKNLGEIVHQLLKHQ
HHHHCCCCCHHHHHHCEEEEEEECCEEEECHHHHHHHHHEEEEECCCHHHHHHHHHHHHC
RKPQTPVAIIKNCATPKQQIWISNLSNIAKQTYAEYLSPCVIIVGEVVRLRDYLQYPNNM
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VREKSEAKTEIAPLANKTILVTRSAGQSSKFSELLKKQGAKVIEMPALVITPPSSWHDLD
HHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCEEECCEEEEECCCCCCEEE
LGITNINSFDWLILTSTNGVDYFFERLAEKGKDSRALAGVKIAVVGKKTAESLKTCGLVP
CEEECCCCEEEEEEECCCCHHHHHHHHHHCCCCCCEECCEEEEEECCHHHHHHHHCCCCC
DFIPPNFVADSLVANFPEKLSGKKILFPRVETGGREVLVKELNSQGAEVIEVAAYESSCP
CCCCCHHHHHHHHHCCHHHHCCCEEEECCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCC
ENILPEALLALQNYHIDVITFASSKTVKNFCYLIGENKDKLPEDLLENICIASIGPQTSQ
HHHHHHHHHHHHCCCEEEEEECCCHHHHHHHEEECCCCCCCHHHHHHHHHEECCCCCCHH
SCHSLLGRVDVEAQEYTLDGLTKAIIEWVRVA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8123787 [H]