Click here to switch to the map view.

The map label for this gene is tilS [H]

Identifier: 113476777

GI number: 113476777

Start: 4986242

End: 4987276

Strand: Reverse

Name: tilS [H]

Synonym: Tery_3251

Alternate gene names: 113476777

Gene position: 4987276-4986242 (Counterclockwise)

Preceding gene: 113476778

Following gene: 113476775

Centisome position: 64.35

GC content: 34.59

Gene sequence:

>1035_bases
ATGCCATATCATAAATCTTCCCACCAAAATTGGACACCACTCCATGCCCAACTACATCAAACATTACGTCAAAGAAAATT
ATTACCAAAAAATCAAAGAATATTAATCGCAGTATCCGGTGGCCAGGATTCTCTATGTCTAACTAAATTATTATTAGATT
TACAATCAAAATGGGGTTGGTATTTAGCAGTTGCTCATTGTGACCATAAATGGCGATCTGACTCAGAAGCTAATGCTAAC
TATGTCAAAAAAATAGCCATAAATTGGCAACTTCCATCTTATGTAGAAACAGCAGATAAAATTTATAAAAGTGAAGCAGA
AGCTAGAAAATGGCGTTATCAAGTTTTGAGTAAAATTGCTTTAGAATTTAATTTTCAATACATTGTAACTGGCCATACAG
CTAGCGATCGCGCAGAAACTCTCTTATATAATCTCATTCGTGGTAGTGGAACTGAAGGCCTACAAGCTCTAACTTGGCAA
CGTCCCCTAATTGAGAATTTCTTACCCTTACTTGTACGTCCTCTATTAGAAATTACTCGCACCCAAACAAGTAACTTTTG
TCAAGAGCAAAAAATCAGAATTTGGGAAGATTCTACAAATCAGGATTTGCATTATGCTAGAAATCGTATTCGTCTGGAAT
TATTTCCTATTTTACGTCAGTTTAATCCTCAAGTTGAACTAGCATTAGCAAAAACAGCAGAAATACTTAGTGCTGAAGTT
AAATATTTTGAAAATGAGACAGAACAGTTATTAGAAAGGGTGACAAAAATAGATACAAATAATTTCAATCTTTCTCAATT
TGAATCGCAACCAGTCAAATATTTTATCAATCGTTTAGTATTACTTCAAGTACCTCTAGCATTACAACGTAGAGTCATGA
GACATATCTTACAGAAAGTTTTACCTGTAAATCCTAACTTCAGTCATATTGAAAAGCTAACAACCCTAGTTAATGCTCCT
AACCATTCTCAAACAGACCCTTTTCCTGGAGGAGCGATCGCCAAAGTTAAACACCCCTGGATTATTCTAGTATAA

Upstream 100 bases:

>100_bases
AGCTTAGGTTACAAATATGGTCTAAGGAATTTGACAACATTTTAATACCAGGAATTCAATTCGGATAGAGGGGAAAAAAT
GCTAACAGTACCTTAAAATT

Downstream 100 bases:

>100_bases
ATAACTACTACTCCTTATTTAATTCGACAAAATTCTTTTTTAAAATTACCATTATTATTGAAAAGCAGTTAAAAATTTTC
TGGTGCAGTATTTCAGTTGC

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MPYHKSSHQNWTPLHAQLHQTLRQRKLLPKNQRILIAVSGGQDSLCLTKLLLDLQSKWGWYLAVAHCDHKWRSDSEANAN
YVKKIAINWQLPSYVETADKIYKSEAEARKWRYQVLSKIALEFNFQYIVTGHTASDRAETLLYNLIRGSGTEGLQALTWQ
RPLIENFLPLLVRPLLEITRTQTSNFCQEQKIRIWEDSTNQDLHYARNRIRLELFPILRQFNPQVELALAKTAEILSAEV
KYFENETEQLLERVTKIDTNNFNLSQFESQPVKYFINRLVLLQVPLALQRRVMRHILQKVLPVNPNFSHIEKLTTLVNAP
NHSQTDPFPGGAIAKVKHPWIILV

Sequences:

>Translated_344_residues
MPYHKSSHQNWTPLHAQLHQTLRQRKLLPKNQRILIAVSGGQDSLCLTKLLLDLQSKWGWYLAVAHCDHKWRSDSEANAN
YVKKIAINWQLPSYVETADKIYKSEAEARKWRYQVLSKIALEFNFQYIVTGHTASDRAETLLYNLIRGSGTEGLQALTWQ
RPLIENFLPLLVRPLLEITRTQTSNFCQEQKIRIWEDSTNQDLHYARNRIRLELFPILRQFNPQVELALAKTAEILSAEV
KYFENETEQLLERVTKIDTNNFNLSQFESQPVKYFINRLVLLQVPLALQRRVMRHILQKVLPVNPNFSHIEKLTTLVNAP
NHSQTDPFPGGAIAKVKHPWIILV
>Mature_343_residues
PYHKSSHQNWTPLHAQLHQTLRQRKLLPKNQRILIAVSGGQDSLCLTKLLLDLQSKWGWYLAVAHCDHKWRSDSEANANY
VKKIAINWQLPSYVETADKIYKSEAEARKWRYQVLSKIALEFNFQYIVTGHTASDRAETLLYNLIRGSGTEGLQALTWQR
PLIENFLPLLVRPLLEITRTQTSNFCQEQKIRIWEDSTNQDLHYARNRIRLELFPILRQFNPQVELALAKTAEILSAEVK
YFENETEQLLERVTKIDTNNFNLSQFESQPVKYFINRLVLLQVPLALQRRVMRHILQKVLPVNPNFSHIEKLTTLVNAPN
HSQTDPFPGGAIAKVKHPWIILV

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=217, Percent_Identity=33.6405529953917, Blast_Score=98, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 40074; Mature: 39942

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYHKSSHQNWTPLHAQLHQTLRQRKLLPKNQRILIAVSGGQDSLCLTKLLLDLQSKWGW
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCE
YLAVAHCDHKWRSDSEANANYVKKIAINWQLPSYVETADKIYKSEAEARKWRYQVLSKIA
EEEEEECCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEFNFQYIVTGHTASDRAETLLYNLIRGSGTEGLQALTWQRPLIENFLPLLVRPLLEITR
HHCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHH
TQTSNFCQEQKIRIWEDSTNQDLHYARNRIRLELFPILRQFNPQVELALAKTAEILSAEV
HHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
KYFENETEQLLERVTKIDTNNFNLSQFESQPVKYFINRLVLLQVPLALQRRVMRHILQKV
HHHHCHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LPVNPNFSHIEKLTTLVNAPNHSQTDPFPGGAIAKVKHPWIILV
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCEEEEC
>Mature Secondary Structure 
PYHKSSHQNWTPLHAQLHQTLRQRKLLPKNQRILIAVSGGQDSLCLTKLLLDLQSKWGW
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCE
YLAVAHCDHKWRSDSEANANYVKKIAINWQLPSYVETADKIYKSEAEARKWRYQVLSKIA
EEEEEECCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEFNFQYIVTGHTASDRAETLLYNLIRGSGTEGLQALTWQRPLIENFLPLLVRPLLEITR
HHCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHH
TQTSNFCQEQKIRIWEDSTNQDLHYARNRIRLELFPILRQFNPQVELALAKTAEILSAEV
HHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
KYFENETEQLLERVTKIDTNNFNLSQFESQPVKYFINRLVLLQVPLALQRRVMRHILQKV
HHHHCHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LPVNPNFSHIEKLTTLVNAPNHSQTDPFPGGAIAKVKHPWIILV
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]