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The map label for this gene is truB

Identifier: 113475954

GI number: 113475954

Start: 3592521

End: 3593426

Strand: Direct

Name: truB

Synonym: Tery_2319

Alternate gene names: 113475954

Gene position: 3592521-3593426 (Clockwise)

Preceding gene: 113475953

Following gene: 113475956

Centisome position: 46.35

GC content: 37.2

Gene sequence:

>906_bases
ATGCAAGGATTTCTCAATCTTAACAAACCATCGGGCATGACTTCCCATGACTGTGTAGCAAAAGTACGAAAGCTACTACG
TCTCAAGCGTGTTGGACATGGAGGAACTCTTGACCCTGCTGCTACTGGTGTTTTACCTATTGCAGTGGGAAAAGCAACCA
GGTTATTACAGTTTCTACCTTCAGAAAAAGCTTATTTTGGTACAGTTAAATTTGGTATAATTACTACTACTGATGACTTA
GAGGGAGAAATTGTTCAATCTGCACCAGTACCAGAATTAATGTTGTCTGATATTGAAAAAGTATTACCAAATTTCCTAGG
TAAAATAGAGCAAGTGCCACCTAGTTATAGTGCAATACAGGTAGAAGGAAAGCGACTTTATGATTTAGCGCGTCAGGGAA
AAACAGTTGAGGTTCCTACCCGTATTGTTGAAGTGTTTAATATAAAAATTCTTGACTGGCGAAGTGGAGATTTTCCAGAA
GTAGACTTGGCGATCGCTTGTGGTACAGGAACTTATATTCGAGCGATCGCTCGCGATCTAGGTAGAACTTTAAGTATTGG
CGGAACTTTAGCTGCTTTAACCAGAATAGAAAGTAGTGGTTTTTCTGAGGAAAATAGTCTCACTTTTGCAGAAGTGGAAA
ATCAACTACAGGAAAATAAGTTTAGTCTTTTATCTATAGAAATAGCATTGGCACATTTACCAGTGATAGTCTTAATTACT
GAAGATGCAAAAAGATGGTGTCAAGGTCAACGTTTACCCTATCAAGTAAAATGGCAGATAGAAACTAAAAAAGCTGAGCA
CTATTTCCGAGTATACAATTATAAAGGTGACTTTCTAGGTATTGGGCAGTTAATCAAATCTGAAGAAAATTTATTATTAG
CACCTCAAGTTGTAATTAGTAATTAG

Upstream 100 bases:

>100_bases
TTTTCTGGCAATAGTTATTGGAGTCGATGAAGAGGACCTTGACTTCATAACTATCTGAGACAACATGATCACAAAAGATT
AAGTTTGAAATAACAATATA

Downstream 100 bases:

>100_bases
AGTCGCTTTGGAATTGATAAGGTATAAAGTTTTACGAGTTTAGGAGTTTAGAGAATAAGTAATATTAAACATGGTAGCGT
TAGTATAATTCCATATAGAG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MQGFLNLNKPSGMTSHDCVAKVRKLLRLKRVGHGGTLDPAATGVLPIAVGKATRLLQFLPSEKAYFGTVKFGIITTTDDL
EGEIVQSAPVPELMLSDIEKVLPNFLGKIEQVPPSYSAIQVEGKRLYDLARQGKTVEVPTRIVEVFNIKILDWRSGDFPE
VDLAIACGTGTYIRAIARDLGRTLSIGGTLAALTRIESSGFSEENSLTFAEVENQLQENKFSLLSIEIALAHLPVIVLIT
EDAKRWCQGQRLPYQVKWQIETKKAEHYFRVYNYKGDFLGIGQLIKSEENLLLAPQVVISN

Sequences:

>Translated_301_residues
MQGFLNLNKPSGMTSHDCVAKVRKLLRLKRVGHGGTLDPAATGVLPIAVGKATRLLQFLPSEKAYFGTVKFGIITTTDDL
EGEIVQSAPVPELMLSDIEKVLPNFLGKIEQVPPSYSAIQVEGKRLYDLARQGKTVEVPTRIVEVFNIKILDWRSGDFPE
VDLAIACGTGTYIRAIARDLGRTLSIGGTLAALTRIESSGFSEENSLTFAEVENQLQENKFSLLSIEIALAHLPVIVLIT
EDAKRWCQGQRLPYQVKWQIETKKAEHYFRVYNYKGDFLGIGQLIKSEENLLLAPQVVISN
>Mature_301_residues
MQGFLNLNKPSGMTSHDCVAKVRKLLRLKRVGHGGTLDPAATGVLPIAVGKATRLLQFLPSEKAYFGTVKFGIITTTDDL
EGEIVQSAPVPELMLSDIEKVLPNFLGKIEQVPPSYSAIQVEGKRLYDLARQGKTVEVPTRIVEVFNIKILDWRSGDFPE
VDLAIACGTGTYIRAIARDLGRTLSIGGTLAALTRIESSGFSEENSLTFAEVENQLQENKFSLLSIEIALAHLPVIVLIT
EDAKRWCQGQRLPYQVKWQIETKKAEHYFRVYNYKGDFLGIGQLIKSEENLLLAPQVVISN

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=250, Percent_Identity=33.2, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI4503337, Length=208, Percent_Identity=30.7692307692308, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI215599015, Length=208, Percent_Identity=30.7692307692308, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI2367200, Length=219, Percent_Identity=37.4429223744292, Blast_Score=145, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17553978, Length=206, Percent_Identity=28.6407766990291, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324037, Length=146, Percent_Identity=38.3561643835616, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323204, Length=215, Percent_Identity=30.2325581395349, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281364189, Length=206, Percent_Identity=31.5533980582524, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364187, Length=206, Percent_Identity=31.5533980582524, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364185, Length=206, Percent_Identity=31.5533980582524, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364183, Length=206, Percent_Identity=31.5533980582524, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI62471759, Length=206, Percent_Identity=31.5533980582524, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17975520, Length=206, Percent_Identity=31.5533980582524, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_TRIEI (Q112N2)

Other databases:

- EMBL:   CP000393
- RefSeq:   YP_722015.1
- ProteinModelPortal:   Q112N2
- SMR:   Q112N2
- STRING:   Q112N2
- GeneID:   4245201
- GenomeReviews:   CP000393_GR
- KEGG:   ter:Tery_2319
- NMPDR:   fig|203124.1.peg.4799
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- PhylomeDB:   Q112N2
- ProtClustDB:   PRK02755
- BioCyc:   TERY203124:TERY_2319-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 33313; Mature: 33313

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: ACT_SITE 38-38

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGFLNLNKPSGMTSHDCVAKVRKLLRLKRVGHGGTLDPAATGVLPIAVGKATRLLQFLP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCEEEEEHHHHHHHHHHCC
SEKAYFGTVKFGIITTTDDLEGEIVQSAPVPELMLSDIEKVLPNFLGKIEQVPPSYSAIQ
CCCCEEEEEEEEEEEECCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
VEGKRLYDLARQGKTVEVPTRIVEVFNIKILDWRSGDFPEVDLAIACGTGTYIRAIARDL
ECCHHHHHHHHCCCEEEHHHHHHHHHEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHH
GRTLSIGGTLAALTRIESSGFSEENSLTFAEVENQLQENKFSLLSIEIALAHLPVIVLIT
CCEEECCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCEEEEEEEEEEHHCCEEEEEE
EDAKRWCQGQRLPYQVKWQIETKKAEHYFRVYNYKGDFLGIGQLIKSEENLLLAPQVVIS
CCHHHHHCCCCCCEEEEEEEECCCCCCEEEEEECCCCEEEHHHHHCCCCCEEEECEEEEC
N
C
>Mature Secondary Structure
MQGFLNLNKPSGMTSHDCVAKVRKLLRLKRVGHGGTLDPAATGVLPIAVGKATRLLQFLP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCEEEEEHHHHHHHHHHCC
SEKAYFGTVKFGIITTTDDLEGEIVQSAPVPELMLSDIEKVLPNFLGKIEQVPPSYSAIQ
CCCCEEEEEEEEEEEECCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
VEGKRLYDLARQGKTVEVPTRIVEVFNIKILDWRSGDFPEVDLAIACGTGTYIRAIARDL
ECCHHHHHHHHCCCEEEHHHHHHHHHEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHH
GRTLSIGGTLAALTRIESSGFSEENSLTFAEVENQLQENKFSLLSIEIALAHLPVIVLIT
CCEEECCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCEEEEEEEEEEHHCCEEEEEE
EDAKRWCQGQRLPYQVKWQIETKKAEHYFRVYNYKGDFLGIGQLIKSEENLLLAPQVVIS
CCHHHHHCCCCCCEEEEEEEECCCCCCEEEEEECCCCEEEHHHHHCCCCCEEEECEEEEC
N
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA