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The map label for this gene is trmH [C]

Identifier: 113475890

GI number: 113475890

Start: 3498006

End: 3498710

Strand: Direct

Name: trmH [C]

Synonym: Tery_2249

Alternate gene names: 113475890

Gene position: 3498006-3498710 (Clockwise)

Preceding gene: 113475889

Following gene: 113475892

Centisome position: 45.13

GC content: 33.05

Gene sequence:

>705_bases
ATGGAAATAGGAAAAAAACAACAATTAATTACTTATTTAAGTCAATTTATAACTCCAGCTCGTCGGGAAAAAATCTTATC
AGTTTTACAGCAACGAACCCGCGACATTACTGTTATTTTAGAAGATATTTATCAGCCCCACAATGCTAGTGCAGTCCTGA
GAAGTTGTGACAGTTTTGGTGTACAAGATGTGCACATTGTAGAGAATAAAAATATTTTTACAATCACTCAGAGTGTAACT
ATTGGTTCAGATCGTTGGTTAACATTATATCGTTATAATGAACAAAGTATAAACAATACAGAAGAATGTTTAAAGAGATT
AAAAAATGAGGGCTATGTAATAGCAGCAACAACTCCCTATAAAGAGCATATAACCATTGAGGAATTATCTGTAAATACAA
AAGTAGCGTTAATGTTTGGTTCAGAAATAAATGGGTTATCAGAATATGCTAAAAACAATGCAGATGTATCGGTCAAAATT
CCCATGGTGGGGTTTAGTGAAAGTTTTAATATTTCTGTTAGCGCCGCTCTATGTTTATATAATTTGATGAGTCGCCTCAA
AAAAGAGCATAATAATTGGAAATTAACGGAGACAGAAAAATTAGATTTGGAATTAAATTGGTTGCAGAAATCTATTAAAG
GCGGGGAACTTATTGAGAAGAAATTTTTCAGAGATATTTATCAGACTACAGGCGGTGGGGAGTAG

Upstream 100 bases:

>100_bases
TTGTAGTCATATAAATAAAAGCTTTTTCCATAATTTTGAATCTTATTTATTCTGGCTTCACTTCTACTTTATCTACTCAT
ATAGTTGAAATTAATGTTGA

Downstream 100 bases:

>100_bases
GAAATATAGGGAGATAGAAAACAATATTTAGTCAGGTAGGGTGCGTATTTACGGCTTAAATTTAAGCTGTATTTCTCTCA
CGCACCATTTTAGCTGTGTT

Product: tRNA/rRNA methyltransferase SpoU

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MEIGKKQQLITYLSQFITPARREKILSVLQQRTRDITVILEDIYQPHNASAVLRSCDSFGVQDVHIVENKNIFTITQSVT
IGSDRWLTLYRYNEQSINNTEECLKRLKNEGYVIAATTPYKEHITIEELSVNTKVALMFGSEINGLSEYAKNNADVSVKI
PMVGFSESFNISVSAALCLYNLMSRLKKEHNNWKLTETEKLDLELNWLQKSIKGGELIEKKFFRDIYQTTGGGE

Sequences:

>Translated_234_residues
MEIGKKQQLITYLSQFITPARREKILSVLQQRTRDITVILEDIYQPHNASAVLRSCDSFGVQDVHIVENKNIFTITQSVT
IGSDRWLTLYRYNEQSINNTEECLKRLKNEGYVIAATTPYKEHITIEELSVNTKVALMFGSEINGLSEYAKNNADVSVKI
PMVGFSESFNISVSAALCLYNLMSRLKKEHNNWKLTETEKLDLELNWLQKSIKGGELIEKKFFRDIYQTTGGGE
>Mature_234_residues
MEIGKKQQLITYLSQFITPARREKILSVLQQRTRDITVILEDIYQPHNASAVLRSCDSFGVQDVHIVENKNIFTITQSVT
IGSDRWLTLYRYNEQSINNTEECLKRLKNEGYVIAATTPYKEHITIEELSVNTKVALMFGSEINGLSEYAKNNADVSVKI
PMVGFSESFNISVSAALCLYNLMSRLKKEHNNWKLTETEKLDLELNWLQKSIKGGELIEKKFFRDIYQTTGGGE

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=164, Percent_Identity=30.4878048780488, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1790623, Length=145, Percent_Identity=27.5862068965517, Blast_Score=71, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 26779; Mature: 26779

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIGKKQQLITYLSQFITPARREKILSVLQQRTRDITVILEDIYQPHNASAVLRSCDSFG
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHCC
VQDVHIVENKNIFTITQSVTIGSDRWLTLYRYNEQSINNTEECLKRLKNEGYVIAATTPY
CCEEEEEECCCEEEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHCCCCEEEEEECCC
KEHITIEELSVNTKVALMFGSEINGLSEYAKNNADVSVKIPMVGFSESFNISVSAALCLY
CCCEEEEEECCCEEEEEEECCCCCHHHHHHCCCCCEEEEEEEEECCCCCCEEHHHHHHHH
NLMSRLKKEHNNWKLTETEKLDLELNWLQKSIKGGELIEKKFFRDIYQTTGGGE
HHHHHHHHHCCCCEEEECCEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEIGKKQQLITYLSQFITPARREKILSVLQQRTRDITVILEDIYQPHNASAVLRSCDSFG
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHCC
VQDVHIVENKNIFTITQSVTIGSDRWLTLYRYNEQSINNTEECLKRLKNEGYVIAATTPY
CCEEEEEECCCEEEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHCCCCEEEEEECCC
KEHITIEELSVNTKVALMFGSEINGLSEYAKNNADVSVKIPMVGFSESFNISVSAALCLY
CCCEEEEEECCCEEEEEEECCCCCHHHHHHCCCCCEEEEEEEEECCCCCCEEHHHHHHHH
NLMSRLKKEHNNWKLTETEKLDLELNWLQKSIKGGELIEKKFFRDIYQTTGGGE
HHHHHHHHHCCCCEEEECCEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]