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The map label for this gene is rph [H]

Identifier: 113475741

GI number: 113475741

Start: 3258626

End: 3259342

Strand: Direct

Name: rph [H]

Synonym: Tery_2089

Alternate gene names: 113475741

Gene position: 3258626-3259342 (Clockwise)

Preceding gene: 113475738

Following gene: 113475742

Centisome position: 42.05

GC content: 39.05

Gene sequence:

>717_bases
ATGTCTTGGCAACGTCCAGATGGTAGAAAGTCAAACCAATTACGTCCTATTAGTTTTGAATTAGATTACACTAAATTTGC
CACCAGTTCTGTCTTAACCAAGTTTGGTGAGACAAAGGTACTCTGTAATGTCACTATCCAACCAGGAGTACCCAAATTTT
TGGCAGATACAGGCCAGGGTTGGTTAACTGCAGAATACAGAATGTTACCAGCTGCAACTTCCGAACGTCAACAACGGGAA
TTTATGAAATTATCTGGGCGAACTCAAGAAATTCAAAGGTTAATTGGACGCAGTTTAAGAGCTGCTCTAGATATGAATTT
ATTAGGGGAACGTACTATAATAGTAGATGCCGATGTTTTGCAAGCAGATGCAGGTACTCGCACTGTTTCTATTACTGGAG
GATTCGTGGCAGTAGCAAATGCTATTAGCAAGTTGATAGACCAGGGAAAGTTGGAGCGATCGCCTATTTTGCATCAAGTT
GCTGCTGTATCAGTAGGGTTATTAGAAAAAGAACCTTATTTAGATTTGAATTATCCTGAAGATGTCGCCGCAGAAATTGA
TTTTAACGTGGTGATGAATGACCAACCTGCTTTAATTGAGGTTCAGGGAACAGCAGAAATAGGTAGCTTTAGTCGTACTC
AACTTAATCAAATTTTAGATTTAGCAGAAGCAGGAATTAAAAAATTATTTGATGCCCAACGTCAAGTTATCTCTTGA

Upstream 100 bases:

>100_bases
CTCGAAAAAGTATCTGAAGAAGAACTTATAAATTGATGTGAAGGAGCGATTAGCTAGTTAAGTATATAAACTACATAATT
CATAAAAAAAATAATTTATT

Downstream 100 bases:

>100_bases
TTAGATACAAAGTTTTTCAAAGGCTTTATCCTTTTGAGAATCAAAAAAGTGCCAGGATTTTTGCCAAGGCTTGGGCAAAA
AAAATCAAGGGATCATTTTT

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MSWQRPDGRKSNQLRPISFELDYTKFATSSVLTKFGETKVLCNVTIQPGVPKFLADTGQGWLTAEYRMLPAATSERQQRE
FMKLSGRTQEIQRLIGRSLRAALDMNLLGERTIIVDADVLQADAGTRTVSITGGFVAVANAISKLIDQGKLERSPILHQV
AAVSVGLLEKEPYLDLNYPEDVAAEIDFNVVMNDQPALIEVQGTAEIGSFSRTQLNQILDLAEAGIKKLFDAQRQVIS

Sequences:

>Translated_238_residues
MSWQRPDGRKSNQLRPISFELDYTKFATSSVLTKFGETKVLCNVTIQPGVPKFLADTGQGWLTAEYRMLPAATSERQQRE
FMKLSGRTQEIQRLIGRSLRAALDMNLLGERTIIVDADVLQADAGTRTVSITGGFVAVANAISKLIDQGKLERSPILHQV
AAVSVGLLEKEPYLDLNYPEDVAAEIDFNVVMNDQPALIEVQGTAEIGSFSRTQLNQILDLAEAGIKKLFDAQRQVIS
>Mature_237_residues
SWQRPDGRKSNQLRPISFELDYTKFATSSVLTKFGETKVLCNVTIQPGVPKFLADTGQGWLTAEYRMLPAATSERQQREF
MKLSGRTQEIQRLIGRSLRAALDMNLLGERTIIVDADVLQADAGTRTVSITGGFVAVANAISKLIDQGKLERSPILHQVA
AVSVGLLEKEPYLDLNYPEDVAAEIDFNVVMNDQPALIEVQGTAEIGSFSRTQLNQILDLAEAGIKKLFDAQRQVIS

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=210, Percent_Identity=56.1904761904762, Blast_Score=235, Evalue=1e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 26267; Mature: 26136

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWQRPDGRKSNQLRPISFELDYTKFATSSVLTKFGETKVLCNVTIQPGVPKFLADTGQG
CCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHCCCC
WLTAEYRMLPAATSERQQREFMKLSGRTQEIQRLIGRSLRAALDMNLLGERTIIVDADVL
EEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHH
QADAGTRTVSITGGFVAVANAISKLIDQGKLERSPILHQVAAVSVGLLEKEPYLDLNYPE
CCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
DVAAEIDFNVVMNDQPALIEVQGTAEIGSFSRTQLNQILDLAEAGIKKLFDAQRQVIS
HHHHEEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SWQRPDGRKSNQLRPISFELDYTKFATSSVLTKFGETKVLCNVTIQPGVPKFLADTGQG
CCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHCCCC
WLTAEYRMLPAATSERQQREFMKLSGRTQEIQRLIGRSLRAALDMNLLGERTIIVDADVL
EEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHH
QADAGTRTVSITGGFVAVANAISKLIDQGKLERSPILHQVAAVSVGLLEKEPYLDLNYPE
CCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
DVAAEIDFNVVMNDQPALIEVQGTAEIGSFSRTQLNQILDLAEAGIKKLFDAQRQVIS
HHHHEEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA