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The map label for this gene is cbiF [H]

Identifier: 113475244

GI number: 113475244

Start: 2369008

End: 2369859

Strand: Reverse

Name: cbiF [H]

Synonym: Tery_1552

Alternate gene names: 113475244

Gene position: 2369859-2369008 (Counterclockwise)

Preceding gene: 113475245

Following gene: 113475243

Centisome position: 30.58

GC content: 39.79

Gene sequence:

>852_bases
GTGCATCTACCATTCCATTTATCTGAAGATAAAGACTCATATCCGGAAAAATTAATGAACGATACTAAAAGTCTCACACC
AGCAGTTTACATTTTAGGGGCAGGCCCTGGAGCTCCGGACTTATTAACGGTGAAGGCTGACAAAATCTTAACTACAGCAG
ATGTAATTCTATATGCAGATTCTTTGGTACCCAAACAAATGTTAAGGAATGTGCGTCATGATGCTGAGATTATCACAACG
GCTAATAAAACTTTAGAAGAAATTTTACCTTTAATGATTGACCGAGTCAGAAAAGGTCTTTCTGTGGTGCGCCTTCATTC
TGGAGACCCTAGTTTATATGGGGCGGTTTATGAACAAATGCAACTTTTGGCTGAAGCGGGAATTGATTTTGAGATTGTGC
CTGGAATTAGTGCATTTCAGGATGCAGCAGCTAAGTTAAAGGTTGAGTTGACTGTGCCTGGGTTGGTACAGACTATTATT
TTAACAAGAATTAGTGGGAAAGCAACTGCTGTTCCTTCAAGGGAAGAATTGGGTAGTTTGGCAGCTCATGGAGCCAGTTT
ATGTTTATATTTGAGTGCCCGATATGTGTGGGAAGCACAAGAAAAGTTGATGGAACATTATTCGGCTGACATACCTGTGG
CGATATGTTATAGGTTGGGATGGCCTGATGAGAAAATTTGGGTTGTGCCTTTGGAGAAAATGGCCCTTATGACTGAAGAG
AAACAGTTAGTTAGAACCACACTTTATGTGATTAGTCCTGCTTTGGAGAAGCTGGAAAAAGTAGGTCGCTCTGGTCTTTA
TAATCCTGAACATAATCATTTATTTCGAGGAAAACATAACAGAAAAAATTAA

Upstream 100 bases:

>100_bases
TTGCTAGATGTTTTACCCCCAGAAGTTAAAGAAACAAACTGAACTAAACTAAGTGAAAAATTAGAAAAGTCCTAGACATA
AAGCCTAGGACTAAACCAGG

Downstream 100 bases:

>100_bases
ATTTAACTAATAGCAAATGATTCCAAAGTTTAAAGATAAATAGCTTTAATTAATCGAGACTTTATAATTCCTGAAGTGGA
TGGAATTGATTAATTATGAC

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MHLPFHLSEDKDSYPEKLMNDTKSLTPAVYILGAGPGAPDLLTVKADKILTTADVILYADSLVPKQMLRNVRHDAEIITT
ANKTLEEILPLMIDRVRKGLSVVRLHSGDPSLYGAVYEQMQLLAEAGIDFEIVPGISAFQDAAAKLKVELTVPGLVQTII
LTRISGKATAVPSREELGSLAAHGASLCLYLSARYVWEAQEKLMEHYSADIPVAICYRLGWPDEKIWVVPLEKMALMTEE
KQLVRTTLYVISPALEKLEKVGRSGLYNPEHNHLFRGKHNRKN

Sequences:

>Translated_283_residues
MHLPFHLSEDKDSYPEKLMNDTKSLTPAVYILGAGPGAPDLLTVKADKILTTADVILYADSLVPKQMLRNVRHDAEIITT
ANKTLEEILPLMIDRVRKGLSVVRLHSGDPSLYGAVYEQMQLLAEAGIDFEIVPGISAFQDAAAKLKVELTVPGLVQTII
LTRISGKATAVPSREELGSLAAHGASLCLYLSARYVWEAQEKLMEHYSADIPVAICYRLGWPDEKIWVVPLEKMALMTEE
KQLVRTTLYVISPALEKLEKVGRSGLYNPEHNHLFRGKHNRKN
>Mature_283_residues
MHLPFHLSEDKDSYPEKLMNDTKSLTPAVYILGAGPGAPDLLTVKADKILTTADVILYADSLVPKQMLRNVRHDAEIITT
ANKTLEEILPLMIDRVRKGLSVVRLHSGDPSLYGAVYEQMQLLAEAGIDFEIVPGISAFQDAAAKLKVELTVPGLVQTII
LTRISGKATAVPSREELGSLAAHGASLCLYLSARYVWEAQEKLMEHYSADIPVAICYRLGWPDEKIWVVPLEKMALMTEE
KQLVRTTLYVISPALEKLEKVGRSGLYNPEHNHLFRGKHNRKN

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=209, Percent_Identity=33.9712918660287, Blast_Score=98, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6322922, Length=211, Percent_Identity=25.5924170616114, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 31465; Mature: 31465

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLPFHLSEDKDSYPEKLMNDTKSLTPAVYILGAGPGAPDLLTVKADKILTTADVILYAD
CCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHH
SLVPKQMLRNVRHDAEIITTANKTLEEILPLMIDRVRKGLSVVRLHSGDPSLYGAVYEQM
HCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHH
QLLAEAGIDFEIVPGISAFQDAAAKLKVELTVPGLVQTIILTRISGKATAVPSREELGSL
HHHHHCCCCEEEECCCHHHHHHHHEEEEEEECHHHHHHHHHHHCCCCCCCCCCHHHHHHH
AAHGASLCLYLSARYVWEAQEKLMEHYSADIPVAICYRLGWPDEKIWVVPLEKMALMTEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEHHHHHHHHHH
KQLVRTTLYVISPALEKLEKVGRSGLYNPEHNHLFRGKHNRKN
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCC
>Mature Secondary Structure
MHLPFHLSEDKDSYPEKLMNDTKSLTPAVYILGAGPGAPDLLTVKADKILTTADVILYAD
CCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHH
SLVPKQMLRNVRHDAEIITTANKTLEEILPLMIDRVRKGLSVVRLHSGDPSLYGAVYEQM
HCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHH
QLLAEAGIDFEIVPGISAFQDAAAKLKVELTVPGLVQTIILTRISGKATAVPSREELGSL
HHHHHCCCCEEEECCCHHHHHHHHEEEEEEECHHHHHHHHHHHCCCCCCCCCCHHHHHHH
AAHGASLCLYLSARYVWEAQEKLMEHYSADIPVAICYRLGWPDEKIWVVPLEKMALMTEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEHHHHHHHHHH
KQLVRTTLYVISPALEKLEKVGRSGLYNPEHNHLFRGKHNRKN
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]