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The map label for this gene is clpR [H]

Identifier: 113475005

GI number: 113475005

Start: 1930019

End: 1930708

Strand: Direct

Name: clpR [H]

Synonym: Tery_1246

Alternate gene names: 113475005

Gene position: 1930019-1930708 (Clockwise)

Preceding gene: 113475003

Following gene: 113475006

Centisome position: 24.9

GC content: 34.93

Gene sequence:

>690_bases
ATGAAATCACCGATCAAAGCAGTTCAAACAAGCTCTTACTATGGAGACGTCTCTTTTCGTACACCACCCCCTGATTTAGA
ATCTTTAATGTTAAAAGAGCGAATCGTTTATTTGGGGCTACCATTATTTTCATCAGATGAAGTCAAAAGAAATGTCGGCA
TTGATGTTACTCAATTAATTATTGCTCAACTACTTTATTTACAATTTGATAACCCCGAAAAACCAATTTATTTTTATATC
AACTCTACTGGCACTTCTTGGTATACAGGAGATGCAATTGGTTTTGAAACAGAAGCATTTGCTATTTGTGACACCATGAG
TTATGTAAAACCACCAATTCATACAATATGTATTGGTCAAGCTATGGGTACAGCAGCAATGATTTTGTCAGCAGGGACAA
AAGGATATCGGGCAAGTTTACCTCATGCTACAATAGTTCTCAATCAAGCCAAAACAGGAGCAAGGGGTCAAGCAACGGAT
ATTTCCATTAGAGCAAAAGAAGTTTTAGTTAATAAGACAACTTTTTTAGATATTCTCTCGAAAAATACAGGTCAATCAGT
AGAAAAGATTGCTAAAGATACAGATAGAATGCTCTACATGACTCCAGAAATAGCTAAAGAATATGGTCTAATAGATCAAG
TTTTAGCAAGTAGAAAAGAACTACCAAAACCTTTACCTGCTAATGTTTAA

Upstream 100 bases:

>100_bases
CAATTATATCATAACCCATAACCAATTGTATCCACTACAATAATATTGCCTAGGCTAATATAATTAAAAGATAGTACAAT
ACATCAATAGGAATTATCTG

Downstream 100 bases:

>100_bases
GTGATAAACAAAATTAACAAGTTTTGGCAAGTAGAAAAGAACTATCCAGTATCAAGACTCAAAAATTAGGTCTCAAGCCT
TTCCTTCTAAAAGGATAAAA

Product: ATP-dependent Clp protease-like protein

Products: NA

Alternate protein names: Endopeptidase Clp-like [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MKSPIKAVQTSSYYGDVSFRTPPPDLESLMLKERIVYLGLPLFSSDEVKRNVGIDVTQLIIAQLLYLQFDNPEKPIYFYI
NSTGTSWYTGDAIGFETEAFAICDTMSYVKPPIHTICIGQAMGTAAMILSAGTKGYRASLPHATIVLNQAKTGARGQATD
ISIRAKEVLVNKTTFLDILSKNTGQSVEKIAKDTDRMLYMTPEIAKEYGLIDQVLASRKELPKPLPANV

Sequences:

>Translated_229_residues
MKSPIKAVQTSSYYGDVSFRTPPPDLESLMLKERIVYLGLPLFSSDEVKRNVGIDVTQLIIAQLLYLQFDNPEKPIYFYI
NSTGTSWYTGDAIGFETEAFAICDTMSYVKPPIHTICIGQAMGTAAMILSAGTKGYRASLPHATIVLNQAKTGARGQATD
ISIRAKEVLVNKTTFLDILSKNTGQSVEKIAKDTDRMLYMTPEIAKEYGLIDQVLASRKELPKPLPANV
>Mature_229_residues
MKSPIKAVQTSSYYGDVSFRTPPPDLESLMLKERIVYLGLPLFSSDEVKRNVGIDVTQLIIAQLLYLQFDNPEKPIYFYI
NSTGTSWYTGDAIGFETEAFAICDTMSYVKPPIHTICIGQAMGTAAMILSAGTKGYRASLPHATIVLNQAKTGARGQATD
ISIRAKEVLVNKTTFLDILSKNTGQSVEKIAKDTDRMLYMTPEIAKEYGLIDQVLASRKELPKPLPANV

Specific function: Has lost the two conserved residues (Ser and His) proposed to be part of the active site. Therefore it could be inactive [H]

COG id: COG0740

COG function: function code OU; Protease subunit of ATP-dependent Clp proteases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S14 family [H]

Homologues:

Organism=Homo sapiens, GI5174419, Length=191, Percent_Identity=42.4083769633508, Blast_Score=146, Evalue=2e-35,
Organism=Escherichia coli, GI1786641, Length=216, Percent_Identity=39.3518518518519, Blast_Score=150, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17538017, Length=198, Percent_Identity=35.8585858585859, Blast_Score=121, Evalue=3e-28,
Organism=Drosophila melanogaster, GI20129427, Length=191, Percent_Identity=42.4083769633508, Blast_Score=145, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001907 [H]

Pfam domain/function: PF00574 CLP_protease [H]

EC number: 3.4.21.92

Molecular weight: Translated: 25233; Mature: 25233

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSPIKAVQTSSYYGDVSFRTPPPDLESLMLKERIVYLGLPLFSSDEVKRNVGIDVTQLI
CCCCHHHHHCCCEECCEECCCCCCCHHHHHHHHHHHHEECCCCCCCHHHHHCCCCHHHHH
IAQLLYLQFDNPEKPIYFYINSTGTSWYTGDAIGFETEAFAICDTMSYVKPPIHTICIGQ
HHHHHHHCCCCCCCCEEEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHCCCHHEEEHHH
AMGTAAMILSAGTKGYRASLPHATIVLNQAKTGARGQATDISIRAKEVLVNKTTFLDILS
HHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHH
KNTGQSVEKIAKDTDRMLYMTPEIAKEYGLIDQVLASRKELPKPLPANV
CCCCHHHHHHHHCCCCEEEECHHHHHHHCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKSPIKAVQTSSYYGDVSFRTPPPDLESLMLKERIVYLGLPLFSSDEVKRNVGIDVTQLI
CCCCHHHHHCCCEECCEECCCCCCCHHHHHHHHHHHHEECCCCCCCHHHHHCCCCHHHHH
IAQLLYLQFDNPEKPIYFYINSTGTSWYTGDAIGFETEAFAICDTMSYVKPPIHTICIGQ
HHHHHHHCCCCCCCCEEEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHCCCHHEEEHHH
AMGTAAMILSAGTKGYRASLPHATIVLNQAKTGARGQATDISIRAKEVLVNKTTFLDILS
HHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHH
KNTGQSVEKIAKDTDRMLYMTPEIAKEYGLIDQVLASRKELPKPLPANV
CCCCHHHHHHHHCCCCEEEECHHHHHHHCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP imidodiphosphate; dATP degradation, [C]

Metal ions: Ca2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 800 [C]

Specific activity: NA

Km value (mM): 0.21 {ATP}} 1.3 {N-succinyl-Leu-Tyr} [C]

Substrates: NA

Specific reaction: NA

General reaction: Hydrolysis of peptide bonds [C]

Inhibitor: Diisopropyl fluorophosphate ClpAP; Fluorosulfonyl benzoyl adenosine; Kappa-Casein; Mg2+; NEM hydrolysis; Neohydrin; Succinyl -Leu-Tyr4-methyl coumarin7-amide; Xaa-Tyr-Leu-Tyr-Trp [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]