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The map label for this gene is speE [H]

Identifier: 113474699

GI number: 113474699

Start: 1406490

End: 1407422

Strand: Direct

Name: speE [H]

Synonym: Tery_0886

Alternate gene names: 113474699

Gene position: 1406490-1407422 (Clockwise)

Preceding gene: 113474698

Following gene: 113474700

Centisome position: 18.15

GC content: 41.05

Gene sequence:

>933_bases
ATGGCAAATAACATGGTAGCTTCAGATCTGTGGATAAGTGAGCATATTACACCTTGGGATATTTATGTTCATGGCCTTAC
TAAGGTACTTGCCTACAAAAAAACTGCTTACCAAGAAATGTACATTGTAGAAACAGGTATTTATGGTAAGGCACTGGTTC
TTGATGGTAAATGGCAGTCTTGTACAGGGGATGAATTTCTATACCATGAACCCCTGGTCCATCCTGCTATGATTTGTCAT
GGGTCTCCTCGCAAAGTTCTTGTTCTTGGTGGTGGTGAGGGAGCTACTATTCGGGAAGTTTTCCGTTGGCAAAGTGTAGA
AAAAGTGGCCATGGTTGATATTGATGGAGAAGTAGTAGAAGCTTGTCGTCAATATTTGCCGGAGATGCATGCCAATGCTT
TTGATGACCCCCGCCTCCAGTTGGTCATTGGTGATGCTTTAAAATTTTTGGAGACTTTTGGTGAGAGTTGGGATGTGGTC
ATTTCTGATCTTTCTGATCCTATTGAAGAGGGACCATCATTTCAATTGTTTACAAAGGAATATTTTGAAAAAATTCTGCG
GGTACTTACCCCGGGAGGATTTTTTGTTGTACAGGCAGGTCCTGTTTCCCCTGGTGAAATGAAACTTCATGCTCGAATTG
TTAATACAATGAAGGCTGTATTTCCTTTTGTTCAATCTTATAATAGTAATATTCCTACTTATGGTCGACCTTGGGGTTTT
GCACTGGGCTCTAGGAAAATGGTTAATAGTCGCCCTGAACCAGAAATGATTGATAAACTTCTGAAAGAAAAAACTATTGG
TGGGTTCCGGATGTTGGATGGCACTACCTTATTAGGAATGTTGCAGGTACCACTACATCTGCGAGAAGCGATCGCTACTG
AAACAAAAATCTATACTTTAGCAGAACCTCCAAAATTTGGTCGTCCTGCATAG

Upstream 100 bases:

>100_bases
GTTTATAGATTAAATTTATTAAGAATAAATAGTTATTTTATGATAATAAATACAATAATCATCTTGATTTTAATACATAA
GTTATTTAGGAGTAGGCCTT

Downstream 100 bases:

>100_bases
TAATTATTTTTGAGTAAAACTGGAATTATATGATGGGAAGGCAGAGAGTATAGGTGCCTGATATGGGACTTTTTTTGAGA
GTTTTTACCCTAAGCATAAA

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MANNMVASDLWISEHITPWDIYVHGLTKVLAYKKTAYQEMYIVETGIYGKALVLDGKWQSCTGDEFLYHEPLVHPAMICH
GSPRKVLVLGGGEGATIREVFRWQSVEKVAMVDIDGEVVEACRQYLPEMHANAFDDPRLQLVIGDALKFLETFGESWDVV
ISDLSDPIEEGPSFQLFTKEYFEKILRVLTPGGFFVVQAGPVSPGEMKLHARIVNTMKAVFPFVQSYNSNIPTYGRPWGF
ALGSRKMVNSRPEPEMIDKLLKEKTIGGFRMLDGTTLLGMLQVPLHLREAIATETKIYTLAEPPKFGRPA

Sequences:

>Translated_310_residues
MANNMVASDLWISEHITPWDIYVHGLTKVLAYKKTAYQEMYIVETGIYGKALVLDGKWQSCTGDEFLYHEPLVHPAMICH
GSPRKVLVLGGGEGATIREVFRWQSVEKVAMVDIDGEVVEACRQYLPEMHANAFDDPRLQLVIGDALKFLETFGESWDVV
ISDLSDPIEEGPSFQLFTKEYFEKILRVLTPGGFFVVQAGPVSPGEMKLHARIVNTMKAVFPFVQSYNSNIPTYGRPWGF
ALGSRKMVNSRPEPEMIDKLLKEKTIGGFRMLDGTTLLGMLQVPLHLREAIATETKIYTLAEPPKFGRPA
>Mature_309_residues
ANNMVASDLWISEHITPWDIYVHGLTKVLAYKKTAYQEMYIVETGIYGKALVLDGKWQSCTGDEFLYHEPLVHPAMICHG
SPRKVLVLGGGEGATIREVFRWQSVEKVAMVDIDGEVVEACRQYLPEMHANAFDDPRLQLVIGDALKFLETFGESWDVVI
SDLSDPIEEGPSFQLFTKEYFEKILRVLTPGGFFVVQAGPVSPGEMKLHARIVNTMKAVFPFVQSYNSNIPTYGRPWGFA
LGSRKMVNSRPEPEMIDKLLKEKTIGGFRMLDGTTLLGMLQVPLHLREAIATETKIYTLAEPPKFGRPA

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=252, Percent_Identity=32.5396825396825, Blast_Score=136, Evalue=3e-32,
Organism=Escherichia coli, GI1786312, Length=271, Percent_Identity=35.7933579335793, Blast_Score=176, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI212645616, Length=234, Percent_Identity=35.8974358974359, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI25154106, Length=217, Percent_Identity=37.3271889400922, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323175, Length=228, Percent_Identity=36.8421052631579, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6325326, Length=248, Percent_Identity=37.0967741935484, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24645443, Length=252, Percent_Identity=33.3333333333333, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI21355941, Length=181, Percent_Identity=33.7016574585635, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24663238, Length=250, Percent_Identity=25.2, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21358269, Length=242, Percent_Identity=24.7933884297521, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 34800; Mature: 34669

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANNMVASDLWISEHITPWDIYVHGLTKVLAYKKTAYQEMYIVETGIYGKALVLDGKWQS
CCCCEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEECCCCCC
CTGDEFLYHEPLVHPAMICHGSPRKVLVLGGGEGATIREVFRWQSVEKVAMVDIDGEVVE
CCCCCCCCCCCCCCHHHEECCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEECCHHHHH
ACRQYLPEMHANAFDDPRLQLVIGDALKFLETFGESWDVVISDLSDPIEEGPSFQLFTKE
HHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEHHHH
YFEKILRVLTPGGFFVVQAGPVSPGEMKLHARIVNTMKAVFPFVQSYNSNIPTYGRPWGF
HHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
ALGSRKMVNSRPEPEMIDKLLKEKTIGGFRMLDGTTLLGMLQVPLHLREAIATETKIYTL
CCCCCHHHCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCEEEEE
AEPPKFGRPA
CCCCCCCCCC
>Mature Secondary Structure 
ANNMVASDLWISEHITPWDIYVHGLTKVLAYKKTAYQEMYIVETGIYGKALVLDGKWQS
CCCEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEECCCCCC
CTGDEFLYHEPLVHPAMICHGSPRKVLVLGGGEGATIREVFRWQSVEKVAMVDIDGEVVE
CCCCCCCCCCCCCCHHHEECCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEECCHHHHH
ACRQYLPEMHANAFDDPRLQLVIGDALKFLETFGESWDVVISDLSDPIEEGPSFQLFTKE
HHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEHHHH
YFEKILRVLTPGGFFVVQAGPVSPGEMKLHARIVNTMKAVFPFVQSYNSNIPTYGRPWGF
HHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
ALGSRKMVNSRPEPEMIDKLLKEKTIGGFRMLDGTTLLGMLQVPLHLREAIATETKIYTL
CCCCCHHHCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCEEEEE
AEPPKFGRPA
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA