Click here to switch to the map view.

The map label for this gene is kefA [H]

Identifier: 113474640

GI number: 113474640

Start: 1287239

End: 1289170

Strand: Direct

Name: kefA [H]

Synonym: Tery_0812

Alternate gene names: 113474640

Gene position: 1287239-1289170 (Clockwise)

Preceding gene: 113474638

Following gene: 113474642

Centisome position: 16.61

GC content: 40.27

Gene sequence:

>1932_bases
ATGGCAAGACTTCAGAAATATAAGTTGAGATTTGCCCTTGTAGTTAGCATCAGTTTACTATTAGTATTTCTACTCTTTCC
TGTCCAAGCTAAAGATACAACCAGTGTCAAAGTTCCAGTAGTATTAGATGGTCGAGAATTGTTAGAAGTTGGTGCTTTAG
GAAAACTGACAGCTCAGGAGAGAGCTAAATTTACGACATCAATTCTCAAGTCAGCCTTAGCAGATAGTATTGAACGAGGA
ACACCTCCAGATGTGAAAGTGGTTCGCTCTAATCAAAATGTGCTATTGAAAGTCGATAGTCGCCCCTTAGTGGAGATAGC
AGAAGATACCTTCAACTCCATGTACTCCGAAATGCAGGTGCAAATGTGGCAGAAACAACTGCAGCAGGGTTTGGAAAAAA
GTTGGCGGGAACGCACACCAGCTTATACCCGTTGGGCAGTCAAAATGCTGACTATTGCGTTTTTAATTACTCTGTTGCTT
CAGGGGGGACTCGTATTGCTGTTTCGTTATGTTCTACGCCAGAAGATAAAAAATCCTCACAGACGCGGGGGTTCTTTACG
ACTATTAGGTTTGGTATTATTGCAAATAGCAATATGGGCGATATTTATCAATTATGGAGTTCAATTATTTCCCATCACTA
GAGGCTGGTTTTACGAGATTTTTCAACTTTTAGACAATACATTTAATGCCAGAATGTTTCAGTTGGGAGAAGAAACCATT
TCTCTCAACCGAATATTAACTATCGCCCTCACTATCGCTGCACTTTGGATCGGAGTAGGTTGGCTTGTTAGTATCCTCAA
ATCTCGCTTGCTTCCCCTCACAGGAGCAGAAACCAGTCACCAAGATACAATTGCTTTTTTTCTTCAATACGGACTGCTAT
CCGTAGGAGCACTCCTAATTCTCAATGCTGGAGGGGTAGATTTTCAATCTCTAGCTCTCTTACTGGGAGCCCTTGGAGTT
GGTATTGGTTTTGGATTACAAAATATTGTTAAAGACTTCATTTGTGGGTTAATTTTAATTGTTGCCCGTCCGATAAAAGT
TGGAGAGTTGGTTCAGGTAGGAAATTTTCAAGGTTTAGTTCAACGCATCGGTGCTAGAACTACTGATATTTCTAATGTCG
AACGCTATATTATTACTATTCCTAATTCTCGCTTTATTGAAGGGGAAGTTCTCAACTGGAACCGCAGTGGTATAACTAGA
GTAAAAGCTTATGTGAAAATTCCTTATGGAACAGATATTGATTTTGTCTACAAAGTTCTACTTGCTGCGGCACAAGTGGA
ACATCCAGATATTCTCCGCCATCCTCCACCCAAAGTAAAATTTCGGGAATATGTAGAGAATGGGTTGCAGTTTCGGATGG
TGGCTTTTATCCGCGATCCTCTTAAGCAACTAGAGGTGAGAACCCACCTGTACAAACAAATAGAAAAATATTTACGAAAG
TATGGAGTTGAAGTTGCTCGCCGCCGACAAGATATACATCTCAAAGACCTCGATCCTGAAATCTATGGTGGAGAGCGATC
GCAAACTTCTGCAGAAGATCGACTTCTTTCTCAAGAGATGCTATCTATTGAACCACCATCTATTAAAGAAGAGTACGACT
GGGAGGCGATATCTACTGCCATGGGTGGAGAGAATGGAGTTTCTATCAAAGATAGACGGTTTCACTTTAAGATCTTCAAA
AATGTCTTTTTAGGTTCGGAGGCAGTAGAGTGGTTAATGATTAATGAACGAGCTACTAGGGAAGAAGCTATTCTGATGGG
GGAGTTAATGTTGCAACAGGGGATAATTCACCACGTTTTGGATGAACATAATTTTAAGGATGAGCCTCTTTTTTATCGCT
TTTATAATCATGAAGTTGAAGCCAATATTAATACTAATGCTAATGGCTCGGAGCATGGAATTGATTCAAAGTTACAAGAT
GATGCTGAATAA

Upstream 100 bases:

>100_bases
TAAAGACAAAGTTAGGAGTATGTCAAAATATATGATAATATTATACAGTTTTTAATACAATATGCCCCAAATATAAAATT
AATTCTGTACAGGTACCTAT

Downstream 100 bases:

>100_bases
TAATTTTTTTTCTGGTTCTATTCTTTGATCAAGAGTAAGATCATGTCCGGATAATGAGTTATCAAAAAGTATATGGTTTG
TAGTAGGGCTTTAGCTCTAG

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 643; Mature: 642

Protein sequence:

>643_residues
MARLQKYKLRFALVVSISLLLVFLLFPVQAKDTTSVKVPVVLDGRELLEVGALGKLTAQERAKFTTSILKSALADSIERG
TPPDVKVVRSNQNVLLKVDSRPLVEIAEDTFNSMYSEMQVQMWQKQLQQGLEKSWRERTPAYTRWAVKMLTIAFLITLLL
QGGLVLLFRYVLRQKIKNPHRRGGSLRLLGLVLLQIAIWAIFINYGVQLFPITRGWFYEIFQLLDNTFNARMFQLGEETI
SLNRILTIALTIAALWIGVGWLVSILKSRLLPLTGAETSHQDTIAFFLQYGLLSVGALLILNAGGVDFQSLALLLGALGV
GIGFGLQNIVKDFICGLILIVARPIKVGELVQVGNFQGLVQRIGARTTDISNVERYIITIPNSRFIEGEVLNWNRSGITR
VKAYVKIPYGTDIDFVYKVLLAAAQVEHPDILRHPPPKVKFREYVENGLQFRMVAFIRDPLKQLEVRTHLYKQIEKYLRK
YGVEVARRRQDIHLKDLDPEIYGGERSQTSAEDRLLSQEMLSIEPPSIKEEYDWEAISTAMGGENGVSIKDRRFHFKIFK
NVFLGSEAVEWLMINERATREEAILMGELMLQQGIIHHVLDEHNFKDEPLFYRFYNHEVEANINTNANGSEHGIDSKLQD
DAE

Sequences:

>Translated_643_residues
MARLQKYKLRFALVVSISLLLVFLLFPVQAKDTTSVKVPVVLDGRELLEVGALGKLTAQERAKFTTSILKSALADSIERG
TPPDVKVVRSNQNVLLKVDSRPLVEIAEDTFNSMYSEMQVQMWQKQLQQGLEKSWRERTPAYTRWAVKMLTIAFLITLLL
QGGLVLLFRYVLRQKIKNPHRRGGSLRLLGLVLLQIAIWAIFINYGVQLFPITRGWFYEIFQLLDNTFNARMFQLGEETI
SLNRILTIALTIAALWIGVGWLVSILKSRLLPLTGAETSHQDTIAFFLQYGLLSVGALLILNAGGVDFQSLALLLGALGV
GIGFGLQNIVKDFICGLILIVARPIKVGELVQVGNFQGLVQRIGARTTDISNVERYIITIPNSRFIEGEVLNWNRSGITR
VKAYVKIPYGTDIDFVYKVLLAAAQVEHPDILRHPPPKVKFREYVENGLQFRMVAFIRDPLKQLEVRTHLYKQIEKYLRK
YGVEVARRRQDIHLKDLDPEIYGGERSQTSAEDRLLSQEMLSIEPPSIKEEYDWEAISTAMGGENGVSIKDRRFHFKIFK
NVFLGSEAVEWLMINERATREEAILMGELMLQQGIIHHVLDEHNFKDEPLFYRFYNHEVEANINTNANGSEHGIDSKLQD
DAE
>Mature_642_residues
ARLQKYKLRFALVVSISLLLVFLLFPVQAKDTTSVKVPVVLDGRELLEVGALGKLTAQERAKFTTSILKSALADSIERGT
PPDVKVVRSNQNVLLKVDSRPLVEIAEDTFNSMYSEMQVQMWQKQLQQGLEKSWRERTPAYTRWAVKMLTIAFLITLLLQ
GGLVLLFRYVLRQKIKNPHRRGGSLRLLGLVLLQIAIWAIFINYGVQLFPITRGWFYEIFQLLDNTFNARMFQLGEETIS
LNRILTIALTIAALWIGVGWLVSILKSRLLPLTGAETSHQDTIAFFLQYGLLSVGALLILNAGGVDFQSLALLLGALGVG
IGFGLQNIVKDFICGLILIVARPIKVGELVQVGNFQGLVQRIGARTTDISNVERYIITIPNSRFIEGEVLNWNRSGITRV
KAYVKIPYGTDIDFVYKVLLAAAQVEHPDILRHPPPKVKFREYVENGLQFRMVAFIRDPLKQLEVRTHLYKQIEKYLRKY
GVEVARRRQDIHLKDLDPEIYGGERSQTSAEDRLLSQEMLSIEPPSIKEEYDWEAISTAMGGENGVSIKDRRFHFKIFKN
VFLGSEAVEWLMINERATREEAILMGELMLQQGIIHHVLDEHNFKDEPLFYRFYNHEVEANINTNANGSEHGIDSKLQDD
AE

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI189571664, Length=68, Percent_Identity=50, Blast_Score=71, Evalue=4e-12,
Organism=Homo sapiens, GI34452732, Length=80, Percent_Identity=40, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI47578115, Length=75, Percent_Identity=46.6666666666667, Blast_Score=70, Evalue=8e-12,
Organism=Homo sapiens, GI47578117, Length=75, Percent_Identity=46.6666666666667, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI155030204, Length=74, Percent_Identity=37.8378378378378, Blast_Score=68, Evalue=3e-11,
Organism=Homo sapiens, GI155030206, Length=74, Percent_Identity=37.8378378378378, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1786670, Length=238, Percent_Identity=32.3529411764706, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI2367355, Length=231, Percent_Identity=30.3030303030303, Blast_Score=108, Evalue=2e-24,
Organism=Escherichia coli, GI1789291, Length=235, Percent_Identity=24.6808510638298, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6322598, Length=74, Percent_Identity=40.5405405405405, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 73136; Mature: 73005

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS50186 DEP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLQKYKLRFALVVSISLLLVFLLFPVQAKDTTSVKVPVVLDGRELLEVGALGKLTAQE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHHCCCCHHHHHH
RAKFTTSILKSALADSIERGTPPDVKVVRSNQNVLLKVDSRPLVEIAEDTFNSMYSEMQV
HHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
QMWQKQLQQGLEKSWRERTPAYTRWAVKMLTIAFLITLLLQGGLVLLFRYVLRQKIKNPH
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHH
RRGGSLRLLGLVLLQIAIWAIFINYGVQLFPITRGWFYEIFQLLDNTFNARMFQLGEETI
HCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLNRILTIALTIAALWIGVGWLVSILKSRLLPLTGAETSHQDTIAFFLQYGLLSVGALLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHE
LNAGGVDFQSLALLLGALGVGIGFGLQNIVKDFICGLILIVARPIKVGELVQVGNFQGLV
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHH
QRIGARTTDISNVERYIITIPNSRFIEGEVLNWNRSGITRVKAYVKIPYGTDIDFVYKVL
HHHCCCCCCCCCCCEEEEECCCCCEECCEEECCCCCCCEEEEEEEECCCCCCHHHHHHHH
LAAAQVEHPDILRHPPPKVKFREYVENGLQFRMVAFIRDPLKQLEVRTHLYKQIEKYLRK
HHHHHCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGVEVARRRQDIHLKDLDPEIYGGERSQTSAEDRLLSQEMLSIEPPSIKEEYDWEAISTA
HCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
MGGENGVSIKDRRFHFKIFKNVFLGSEAVEWLMINERATREEAILMGELMLQQGIIHHVL
CCCCCCCCCCHHHHHHHHHHHHHCCHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
DEHNFKDEPLFYRFYNHEVEANINTNANGSEHGIDSKLQDDAE
HCCCCCCCCEEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ARLQKYKLRFALVVSISLLLVFLLFPVQAKDTTSVKVPVVLDGRELLEVGALGKLTAQE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHHCCCCHHHHHH
RAKFTTSILKSALADSIERGTPPDVKVVRSNQNVLLKVDSRPLVEIAEDTFNSMYSEMQV
HHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
QMWQKQLQQGLEKSWRERTPAYTRWAVKMLTIAFLITLLLQGGLVLLFRYVLRQKIKNPH
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHH
RRGGSLRLLGLVLLQIAIWAIFINYGVQLFPITRGWFYEIFQLLDNTFNARMFQLGEETI
HCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLNRILTIALTIAALWIGVGWLVSILKSRLLPLTGAETSHQDTIAFFLQYGLLSVGALLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHE
LNAGGVDFQSLALLLGALGVGIGFGLQNIVKDFICGLILIVARPIKVGELVQVGNFQGLV
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHH
QRIGARTTDISNVERYIITIPNSRFIEGEVLNWNRSGITRVKAYVKIPYGTDIDFVYKVL
HHHCCCCCCCCCCCEEEEECCCCCEECCEEECCCCCCCEEEEEEEECCCCCCHHHHHHHH
LAAAQVEHPDILRHPPPKVKFREYVENGLQFRMVAFIRDPLKQLEVRTHLYKQIEKYLRK
HHHHHCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGVEVARRRQDIHLKDLDPEIYGGERSQTSAEDRLLSQEMLSIEPPSIKEEYDWEAISTA
HCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
MGGENGVSIKDRRFHFKIFKNVFLGSEAVEWLMINERATREEAILMGELMLQQGIIHHVL
CCCCCCCCCCHHHHHHHHHHHHHCCHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
DEHNFKDEPLFYRFYNHEVEANINTNANGSEHGIDSKLQDDAE
HCCCCCCCCEEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]