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The map label for this gene is alkJ [H]

Identifier: 110835571

GI number: 110835571

Start: 3066633

End: 3068240

Strand: Direct

Name: alkJ [H]

Synonym: ABO_2710

Alternate gene names: 110835571

Gene position: 3066633-3068240 (Clockwise)

Preceding gene: 110835570

Following gene: 110835599

Centisome position: 98.29

GC content: 52.05

Gene sequence:

>1608_bases
ATGTACGATTACATTATTGTAGGAGCCGGGTCGGCTGGCTGTGTGTTGGCTAACCGCCTTAGTGCGGATACTTCAAAACG
CGTAGCGCTGATCGAAGCCGGCCCACGGGATAAAAATCCCCTAATTCACATGCCTATTGGCATCGCTCTCCTCGCCAACA
ATAGAAAACTCAACTGGGCGCTAGAAACCGAGCCTCAAGAACACTTGAAAGGGCGCCAACTGTTTTGGCCCCGCGGAAAG
ACGCTTGGTGGCTCTTCTTCTATTAATGCCATGGTGTATATCAGGGGCCATAAAGCCGACTACGATCATTGGGGCCAAGT
TGCCGGTAACAACAACCTCTGGGGTTGGGATCGTGCATTAACATTATTTCGTCGGGTGGAAGACAACCAACGCCTTGGCG
CAGACCCATACCACGGCAAAGATGGTGAGCTCACTGTAAGCGAATTAAAATCGATCAACCCGCTGAGCCGTGATTTTGTT
CGAGCAGCGCCTCACGTAGACCTGCCCGTGAACACAGACTTTAACGGCAAATCACAAGACGGATTGGGGCTTTACCAAGT
AACGCAGAAAAATGGCCAGCGCTGGAGTTCAGCGCAAGCATTTTTGCGTGCCGCTGAGAGCCGCTCTAATCTTGACGTGC
TAACCGATGCTCGAGTAACCCGCGTGGCTATGGAGGGTAAGCGAGCGGTTGGCGTGACCCTGAAACAGGGAAGTGAATAT
CGCCAGCTGAGACTCAATGCCGGCGGCGAAGTCATCCTGTCTGGTGGTGCAGTTAATTCACCACAGCTCCTCCTGCTGTC
TGGCATCGGGGATAGTAAAGAGCTTGCGAAACACGGCATTCCGCTAGTTCATCACCTTCCCGAAGTCGGCCAGAATTTGG
CCGATCATCTGGACATCACGATCATGCACACGGCGAACTCTCGTTTGCCCATTGGCGTTGCACCCAGCTTCTTATTCCGT
GGGGTGAGCGCACTTTTCTCCTATATCTTTGCGCGACGTGGTTTTCTTACCAGTAATGTTGCCGAGTCTGGCGGCTTTGT
GAAATCGGACCCTTCGTCTGAGCGACCCAATGTGCAATTTCACTTTTTGCCCACTTACCTGAAGGATCATGGCCGAAAAG
TAATGGCAGGCTATGGCTACACTTTGCACATTTGCGATTTGTTGCCGAAAAGCCGAGGCTTCATTGGATTGCAAAGCCCT
GACCCATTGGCCAATCCGCTGATTCAGCCTAACTATCTGAGCGACCCCGAAGATATCAAAACAATGATATCCGCCATTAA
GTTTGGGCGACGCATTCTCGGAGCACCAACAATGGCGCTTCATAGTAAACGGGAAGTTATGCCGGGAGAGTCCGTATCTA
CGGACGCTCAACTAGCAGACTTTATCCGTGAAAATGCTGAAACCATCTACCACCCTGTTGGCACTTGTCGTATGGGGGCC
GACCCTGATTCCGTTGTCGATCCGGAACTGAAAGTCAGAGGCGTTGAAGGGCTAAGAGTTGTCGATGCCTCGATAATGCC
CAGCTTGGTGGCGGGTAACACGAACGCACCCACAATGATGATTGCCGAAAATGCGGCCGACATCCTGCTGGGAAAGGTCC
AAGTGTAA

Upstream 100 bases:

>100_bases
CCATCTCCTTTTCCCTCCCTACACTGGCTGGGTTCGGCAGCTTATAAATGCTGCTGTTCGTATTTTAGGCTGAGTATTAA
CAAATAATAAGAGAGTTATT

Downstream 100 bases:

>100_bases
ACCCGGGCGACGTAAGCCACCGGGCATACTAATGACATCGGCCATTCAGGCGTACGAACTACTGAAAGCGATATAAGGTA
GTCGTACTTGATCAGATGAT

Product: alcohol dehydrogenase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MYDYIIVGAGSAGCVLANRLSADTSKRVALIEAGPRDKNPLIHMPIGIALLANNRKLNWALETEPQEHLKGRQLFWPRGK
TLGGSSSINAMVYIRGHKADYDHWGQVAGNNNLWGWDRALTLFRRVEDNQRLGADPYHGKDGELTVSELKSINPLSRDFV
RAAPHVDLPVNTDFNGKSQDGLGLYQVTQKNGQRWSSAQAFLRAAESRSNLDVLTDARVTRVAMEGKRAVGVTLKQGSEY
RQLRLNAGGEVILSGGAVNSPQLLLLSGIGDSKELAKHGIPLVHHLPEVGQNLADHLDITIMHTANSRLPIGVAPSFLFR
GVSALFSYIFARRGFLTSNVAESGGFVKSDPSSERPNVQFHFLPTYLKDHGRKVMAGYGYTLHICDLLPKSRGFIGLQSP
DPLANPLIQPNYLSDPEDIKTMISAIKFGRRILGAPTMALHSKREVMPGESVSTDAQLADFIRENAETIYHPVGTCRMGA
DPDSVVDPELKVRGVEGLRVVDASIMPSLVAGNTNAPTMMIAENAADILLGKVQV

Sequences:

>Translated_535_residues
MYDYIIVGAGSAGCVLANRLSADTSKRVALIEAGPRDKNPLIHMPIGIALLANNRKLNWALETEPQEHLKGRQLFWPRGK
TLGGSSSINAMVYIRGHKADYDHWGQVAGNNNLWGWDRALTLFRRVEDNQRLGADPYHGKDGELTVSELKSINPLSRDFV
RAAPHVDLPVNTDFNGKSQDGLGLYQVTQKNGQRWSSAQAFLRAAESRSNLDVLTDARVTRVAMEGKRAVGVTLKQGSEY
RQLRLNAGGEVILSGGAVNSPQLLLLSGIGDSKELAKHGIPLVHHLPEVGQNLADHLDITIMHTANSRLPIGVAPSFLFR
GVSALFSYIFARRGFLTSNVAESGGFVKSDPSSERPNVQFHFLPTYLKDHGRKVMAGYGYTLHICDLLPKSRGFIGLQSP
DPLANPLIQPNYLSDPEDIKTMISAIKFGRRILGAPTMALHSKREVMPGESVSTDAQLADFIRENAETIYHPVGTCRMGA
DPDSVVDPELKVRGVEGLRVVDASIMPSLVAGNTNAPTMMIAENAADILLGKVQV
>Mature_535_residues
MYDYIIVGAGSAGCVLANRLSADTSKRVALIEAGPRDKNPLIHMPIGIALLANNRKLNWALETEPQEHLKGRQLFWPRGK
TLGGSSSINAMVYIRGHKADYDHWGQVAGNNNLWGWDRALTLFRRVEDNQRLGADPYHGKDGELTVSELKSINPLSRDFV
RAAPHVDLPVNTDFNGKSQDGLGLYQVTQKNGQRWSSAQAFLRAAESRSNLDVLTDARVTRVAMEGKRAVGVTLKQGSEY
RQLRLNAGGEVILSGGAVNSPQLLLLSGIGDSKELAKHGIPLVHHLPEVGQNLADHLDITIMHTANSRLPIGVAPSFLFR
GVSALFSYIFARRGFLTSNVAESGGFVKSDPSSERPNVQFHFLPTYLKDHGRKVMAGYGYTLHICDLLPKSRGFIGLQSP
DPLANPLIQPNYLSDPEDIKTMISAIKFGRRILGAPTMALHSKREVMPGESVSTDAQLADFIRENAETIYHPVGTCRMGA
DPDSVVDPELKVRGVEGLRVVDASIMPSLVAGNTNAPTMMIAENAADILLGKVQV

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=543, Percent_Identity=45.1197053406998, Blast_Score=452, Evalue=1e-127,
Organism=Escherichia coli, GI1786503, Length=536, Percent_Identity=40.2985074626866, Blast_Score=395, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17532301, Length=550, Percent_Identity=37.8181818181818, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24642042, Length=568, Percent_Identity=38.9084507042254, Blast_Score=344, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24642055, Length=574, Percent_Identity=38.1533101045296, Blast_Score=335, Evalue=7e-92,
Organism=Drosophila melanogaster, GI45551458, Length=574, Percent_Identity=38.6759581881533, Blast_Score=332, Evalue=4e-91,
Organism=Drosophila melanogaster, GI45549471, Length=574, Percent_Identity=38.6759581881533, Blast_Score=331, Evalue=9e-91,
Organism=Drosophila melanogaster, GI24642059, Length=567, Percent_Identity=39.5061728395062, Blast_Score=330, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24642048, Length=575, Percent_Identity=36.3478260869565, Blast_Score=308, Evalue=5e-84,
Organism=Drosophila melanogaster, GI17137792, Length=555, Percent_Identity=36.3963963963964, Blast_Score=295, Evalue=7e-80,
Organism=Drosophila melanogaster, GI24650267, Length=584, Percent_Identity=36.3013698630137, Blast_Score=292, Evalue=3e-79,
Organism=Drosophila melanogaster, GI24642037, Length=579, Percent_Identity=35.5785837651123, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24642035, Length=578, Percent_Identity=35.2941176470588, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24642039, Length=562, Percent_Identity=33.8078291814947, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI18859995, Length=567, Percent_Identity=33.8624338624339, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI18859993, Length=566, Percent_Identity=32.1554770318021, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24642051, Length=569, Percent_Identity=30.2284710017575, Blast_Score=239, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24645930, Length=581, Percent_Identity=29.4320137693632, Blast_Score=165, Evalue=9e-41,
Organism=Drosophila melanogaster, GI24642057, Length=575, Percent_Identity=31.1304347826087, Blast_Score=153, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 58299; Mature: 58299

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDYIIVGAGSAGCVLANRLSADTSKRVALIEAGPRDKNPLIHMPIGIALLANNRKLNWA
CCEEEEEECCCCCEEEEHHHCCCCCCEEEEEECCCCCCCCEEEECCEEEEEECCCEEEEE
LETEPQEHLKGRQLFWPRGKTLGGSSSINAMVYIRGHKADYDHWGQVAGNNNLWGWDRAL
ECCCHHHHCCCCEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHH
TLFRRVEDNQRLGADPYHGKDGELTVSELKSINPLSRDFVRAAPHVDLPVNTDFNGKSQD
HHHHHHCCCCCCCCCCCCCCCCCEEHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
GLGLYQVTQKNGQRWSSAQAFLRAAESRSNLDVLTDARVTRVAMEGKRAVGVTLKQGSEY
CCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEECCCHHHHHHHCCCCEEEEEECCCCCE
RQLRLNAGGEVILSGGAVNSPQLLLLSGIGDSKELAKHGIPLVHHLPEVGQNLADHLDIT
EEEEECCCCEEEEECCCCCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCEE
IMHTANSRLPIGVAPSFLFRGVSALFSYIFARRGFLTSNVAESGGFVKSDPSSERPNVQF
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCEECCCCCCCCCCEEE
HFLPTYLKDHGRKVMAGYGYTLHICDLLPKSRGFIGLQSPDPLANPLIQPNYLSDPEDIK
EEECHHHHHCCCEEEECCCCEEEEEHHCCCCCCEEECCCCCHHHCCCCCCCCCCCHHHHH
TMISAIKFGRRILGAPTMALHSKREVMPGESVSTDAQLADFIRENAETIYHPVGTCRMGA
HHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCEEECCC
DPDSVVDPELKVRGVEGLRVVDASIMPSLVAGNTNAPTMMIAENAADILLGKVQV
CCCCCCCCCEEEECCCCEEEECHHHCCHHHCCCCCCCEEEEECCCCEEEEEEECC
>Mature Secondary Structure
MYDYIIVGAGSAGCVLANRLSADTSKRVALIEAGPRDKNPLIHMPIGIALLANNRKLNWA
CCEEEEEECCCCCEEEEHHHCCCCCCEEEEEECCCCCCCCEEEECCEEEEEECCCEEEEE
LETEPQEHLKGRQLFWPRGKTLGGSSSINAMVYIRGHKADYDHWGQVAGNNNLWGWDRAL
ECCCHHHHCCCCEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHH
TLFRRVEDNQRLGADPYHGKDGELTVSELKSINPLSRDFVRAAPHVDLPVNTDFNGKSQD
HHHHHHCCCCCCCCCCCCCCCCCEEHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
GLGLYQVTQKNGQRWSSAQAFLRAAESRSNLDVLTDARVTRVAMEGKRAVGVTLKQGSEY
CCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEECCCHHHHHHHCCCCEEEEEECCCCCE
RQLRLNAGGEVILSGGAVNSPQLLLLSGIGDSKELAKHGIPLVHHLPEVGQNLADHLDIT
EEEEECCCCEEEEECCCCCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCEE
IMHTANSRLPIGVAPSFLFRGVSALFSYIFARRGFLTSNVAESGGFVKSDPSSERPNVQF
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCEECCCCCCCCCCEEE
HFLPTYLKDHGRKVMAGYGYTLHICDLLPKSRGFIGLQSPDPLANPLIQPNYLSDPEDIK
EEECHHHHHCCCEEEECCCCEEEEEHHCCCCCCEEECCCCCHHHCCCCCCCCCCCHHHHH
TMISAIKFGRRILGAPTMALHSKREVMPGESVSTDAQLADFIRENAETIYHPVGTCRMGA
HHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCEEECCC
DPDSVVDPELKVRGVEGLRVVDASIMPSLVAGNTNAPTMMIAENAADILLGKVQV
CCCCCCCCCEEEECCCCEEEECHHHCCHHHCCCCCCCEEEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]