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The map label for this gene is pykA [H]

Identifier: 110835482

GI number: 110835482

Start: 2965863

End: 2967356

Strand: Direct

Name: pykA [H]

Synonym: ABO_2621

Alternate gene names: 110835482

Gene position: 2965863-2967356 (Clockwise)

Preceding gene: 110835481

Following gene: 110835483

Centisome position: 95.06

GC content: 59.24

Gene sequence:

>1494_bases
ATGATTGCCGGAAAGATACGCATGCCTCCTCGCACCTCTCAGCACCCGCTGCGCCGCACCAAAATTGTTGCCACCGTTGG
CCCCGCCTCTGCCCCCCTCTCCATGCTCGATACGCTGATTGGGGCGGGCGTTAATGTGTTTCGGCTGAACTTTTCCCACG
GCACCGCAGACACCCATCGCGATACCGCCGAACAGATTCGCCATTGCGCCGAGCAACGCGGCGAACACGTAGCGATACTG
GCCGACCTGCAAGGGCCTAAGATCCGCATTGGCTGCTTTCGCGACGGCCCCATCATGCTCACCGCCGGCCAACCCTTTAC
GCTTGATACTGCTCTGTCAGATGACGCAGGCGATACCGAGCAGGTCGGCGTCAGTTACCCACCACTACCCAGCGACTGTG
AACCCGGCGATATCCTGCTGCTCAATGACGGCCTGCTAGAACTCGAAGTAAACACCATCAACCGCACCCGGGTGGAATGC
ACGGTGCGCGTGGGCGGTGAACTCTCGGATCATAAAGGCGTAAACCGCAAAGGTGGCGGCCTCAGTGCCCGCGCCCTTAC
CGAGAAAGACCTGGCGGATATCATCACCGCCGCCGACATGGAGGTGGATTTTCTCGCGCTCTCCTTTCCCCGCGACGCGG
AGGATGTAAACGAAGCACGCCGCCGTTATCAGGAAGCCGGCGGCAAAGGTGACATCATTGCCAAAATCGAACGCGCCGAA
GCCGTGGCAGACGACGACACCTTGGATGCCATTATCAATGCCTCCGACGGAGTGATGGTGGCGCGTGGCGATTTGGCCGT
GGAAATTGGTGATGCGGAACTTGTCGGCGTGCAGAAGCACATCATCCGCCGGGCACGAGCGTTAAATCGCTTCGTGATTA
CTGCCACCCAGATGATGGAATCCATGATCCACAGCCCACAACCCACCCGGGCAGAGGTCTCAGACGTAGCCAACGCGGTA
CTCGACGGCACCGACGCCGTCATGCTGTCAGCGGAAACCGCGGTCGGCGACTTTCCCAAAGAAACCGTACAGGCCATGGA
CCGGATCATCCTTGGCGCCGAGCGTACCTGGCAGGAACGCAGGGCTGATCGGCCGATCAACGACAACGCCGACAACATTG
ACGAAGTGATCGCCATGGCCGCCATGCAGGCAGCCAACCGACTCAGCAACGTGGCCGCCATTATTGCCATGACGGAAACT
GGTAACACCCCGCGGCTAATGTCTCGATTACGCTCTGGCATGCCCATCTATGCATTCACCCCCTACCCCTCCAGCCAGCG
CCGAGTCGCCATTTTACGTGGTGTATATGCCATGCCCTTCGACAGTCACGCACTGGCTAGCGACACCGTCAACCGCAAAG
CGGTGAGCATGTTAGAAGACATGGGCGTAGTGCAGACTGGCGACCGGATACTGATCACCAAGGGCAATTATGCCGACGCC
CACGGTGGCACCAATACCCTGAAAATCGTCGAGGTCGGCAGTAACATTCAATAA

Upstream 100 bases:

>100_bases
CACCGGCCTGCGAGACCCTTTTGAGAAATCATGCTGACACCACAAAGAAGCCTGCAGACTGTCATCGTTCGTTCGCTTCT
GCGTGGTATGCTAATTCGAA

Downstream 100 bases:

>100_bases
TTCTTTTGCGGAGCCTTCATGCCATTTCCGTTGTGAAGGCTCCGGCTCTGCACCCTAAAGAGCAAAACGCTCGCTCTGCC
AAAAAAACCGCAAATTTTTT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MIAGKIRMPPRTSQHPLRRTKIVATVGPASAPLSMLDTLIGAGVNVFRLNFSHGTADTHRDTAEQIRHCAEQRGEHVAIL
ADLQGPKIRIGCFRDGPIMLTAGQPFTLDTALSDDAGDTEQVGVSYPPLPSDCEPGDILLLNDGLLELEVNTINRTRVEC
TVRVGGELSDHKGVNRKGGGLSARALTEKDLADIITAADMEVDFLALSFPRDAEDVNEARRRYQEAGGKGDIIAKIERAE
AVADDDTLDAIINASDGVMVARGDLAVEIGDAELVGVQKHIIRRARALNRFVITATQMMESMIHSPQPTRAEVSDVANAV
LDGTDAVMLSAETAVGDFPKETVQAMDRIILGAERTWQERRADRPINDNADNIDEVIAMAAMQAANRLSNVAAIIAMTET
GNTPRLMSRLRSGMPIYAFTPYPSSQRRVAILRGVYAMPFDSHALASDTVNRKAVSMLEDMGVVQTGDRILITKGNYADA
HGGTNTLKIVEVGSNIQ

Sequences:

>Translated_497_residues
MIAGKIRMPPRTSQHPLRRTKIVATVGPASAPLSMLDTLIGAGVNVFRLNFSHGTADTHRDTAEQIRHCAEQRGEHVAIL
ADLQGPKIRIGCFRDGPIMLTAGQPFTLDTALSDDAGDTEQVGVSYPPLPSDCEPGDILLLNDGLLELEVNTINRTRVEC
TVRVGGELSDHKGVNRKGGGLSARALTEKDLADIITAADMEVDFLALSFPRDAEDVNEARRRYQEAGGKGDIIAKIERAE
AVADDDTLDAIINASDGVMVARGDLAVEIGDAELVGVQKHIIRRARALNRFVITATQMMESMIHSPQPTRAEVSDVANAV
LDGTDAVMLSAETAVGDFPKETVQAMDRIILGAERTWQERRADRPINDNADNIDEVIAMAAMQAANRLSNVAAIIAMTET
GNTPRLMSRLRSGMPIYAFTPYPSSQRRVAILRGVYAMPFDSHALASDTVNRKAVSMLEDMGVVQTGDRILITKGNYADA
HGGTNTLKIVEVGSNIQ
>Mature_497_residues
MIAGKIRMPPRTSQHPLRRTKIVATVGPASAPLSMLDTLIGAGVNVFRLNFSHGTADTHRDTAEQIRHCAEQRGEHVAIL
ADLQGPKIRIGCFRDGPIMLTAGQPFTLDTALSDDAGDTEQVGVSYPPLPSDCEPGDILLLNDGLLELEVNTINRTRVEC
TVRVGGELSDHKGVNRKGGGLSARALTEKDLADIITAADMEVDFLALSFPRDAEDVNEARRRYQEAGGKGDIIAKIERAE
AVADDDTLDAIINASDGVMVARGDLAVEIGDAELVGVQKHIIRRARALNRFVITATQMMESMIHSPQPTRAEVSDVANAV
LDGTDAVMLSAETAVGDFPKETVQAMDRIILGAERTWQERRADRPINDNADNIDEVIAMAAMQAANRLSNVAAIIAMTET
GNTPRLMSRLRSGMPIYAFTPYPSSQRRVAILRGVYAMPFDSHALASDTVNRKAVSMLEDMGVVQTGDRILITKGNYADA
HGGTNTLKIVEVGSNIQ

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=503, Percent_Identity=35.7852882703777, Blast_Score=262, Evalue=7e-70,
Organism=Homo sapiens, GI33286420, Length=503, Percent_Identity=35.7852882703777, Blast_Score=262, Evalue=7e-70,
Organism=Homo sapiens, GI33286418, Length=509, Percent_Identity=35.9528487229862, Blast_Score=259, Evalue=4e-69,
Organism=Homo sapiens, GI10835121, Length=497, Percent_Identity=36.0160965794769, Blast_Score=257, Evalue=1e-68,
Organism=Homo sapiens, GI32967597, Length=497, Percent_Identity=36.0160965794769, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI310128732, Length=302, Percent_Identity=35.7615894039735, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI310128730, Length=302, Percent_Identity=35.7615894039735, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI310128736, Length=248, Percent_Identity=34.2741935483871, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI310128734, Length=248, Percent_Identity=34.2741935483871, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=33.4883720930233, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=54.3933054393305, Blast_Score=487, Evalue=1e-139,
Organism=Escherichia coli, GI1787965, Length=479, Percent_Identity=37.5782881002088, Blast_Score=277, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17544584, Length=493, Percent_Identity=36.7139959432049, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17506829, Length=497, Percent_Identity=36.0160965794769, Blast_Score=244, Evalue=8e-65,
Organism=Caenorhabditis elegans, GI71984413, Length=497, Percent_Identity=36.0160965794769, Blast_Score=244, Evalue=9e-65,
Organism=Caenorhabditis elegans, GI71984406, Length=497, Percent_Identity=36.0160965794769, Blast_Score=244, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI17506831, Length=497, Percent_Identity=36.0160965794769, Blast_Score=243, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6324923, Length=489, Percent_Identity=33.7423312883436, Blast_Score=241, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6319279, Length=494, Percent_Identity=34.412955465587, Blast_Score=241, Evalue=1e-64,
Organism=Drosophila melanogaster, GI28571814, Length=492, Percent_Identity=35.3658536585366, Blast_Score=265, Evalue=4e-71,
Organism=Drosophila melanogaster, GI24648964, Length=492, Percent_Identity=35.3658536585366, Blast_Score=265, Evalue=5e-71,
Organism=Drosophila melanogaster, GI24581235, Length=488, Percent_Identity=33.6065573770492, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24648966, Length=451, Percent_Identity=35.4767184035477, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24646914, Length=315, Percent_Identity=35.2380952380952, Blast_Score=154, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 53607; Mature: 53607

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAGKIRMPPRTSQHPLRRTKIVATVGPASAPLSMLDTLIGAGVNVFRLNFSHGTADTHR
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCH
DTAEQIRHCAEQRGEHVAILADLQGPKIRIGCFRDGPIMLTAGQPFTLDTALSDDAGDTE
HHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCCEEEECCCCEEEECCCCCCCCCCH
QVGVSYPPLPSDCEPGDILLLNDGLLELEVNTINRTRVECTVRVGGELSDHKGVNRKGGG
HCCCCCCCCCCCCCCCCEEEEECCEEEEEEEECCCEEEEEEEEECCCCCCCCCCCCCCCC
LSARALTEKDLADIITAADMEVDFLALSFPRDAEDVNEARRRYQEAGGKGDIIAKIERAE
CEEHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHH
AVADDDTLDAIINASDGVMVARGDLAVEIGDAELVGVQKHIIRRARALNRFVITATQMME
HHCCCCHHHHHEECCCCEEEEECCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
SMIHSPQPTRAEVSDVANAVLDGTDAVMLSAETAVGDFPKETVQAMDRIILGAERTWQER
HHHCCCCCCHHHHHHHHHHHHCCCCEEEEEECHHCCCCCHHHHHHHHHHHHCCHHHHHHH
RADRPINDNADNIDEVIAMAAMQAANRLSNVAAIIAMTETGNTPRLMSRLRSGMPIYAFT
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCHHHHHHHHCCCCEEEEC
PYPSSQRRVAILRGVYAMPFDSHALASDTVNRKAVSMLEDMGVVQTGDRILITKGNYADA
CCCCCCCEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHCCCEECCCEEEEEECCCCCC
HGGTNTLKIVEVGSNIQ
CCCCCEEEEEECCCCCC
>Mature Secondary Structure
MIAGKIRMPPRTSQHPLRRTKIVATVGPASAPLSMLDTLIGAGVNVFRLNFSHGTADTHR
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCH
DTAEQIRHCAEQRGEHVAILADLQGPKIRIGCFRDGPIMLTAGQPFTLDTALSDDAGDTE
HHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCCEEEECCCCEEEECCCCCCCCCCH
QVGVSYPPLPSDCEPGDILLLNDGLLELEVNTINRTRVECTVRVGGELSDHKGVNRKGGG
HCCCCCCCCCCCCCCCCEEEEECCEEEEEEEECCCEEEEEEEEECCCCCCCCCCCCCCCC
LSARALTEKDLADIITAADMEVDFLALSFPRDAEDVNEARRRYQEAGGKGDIIAKIERAE
CEEHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHH
AVADDDTLDAIINASDGVMVARGDLAVEIGDAELVGVQKHIIRRARALNRFVITATQMME
HHCCCCHHHHHEECCCCEEEEECCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
SMIHSPQPTRAEVSDVANAVLDGTDAVMLSAETAVGDFPKETVQAMDRIILGAERTWQER
HHHCCCCCCHHHHHHHHHHHHCCCCEEEEEECHHCCCCCHHHHHHHHHHHHCCHHHHHHH
RADRPINDNADNIDEVIAMAAMQAANRLSNVAAIIAMTETGNTPRLMSRLRSGMPIYAFT
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCHHHHHHHHCCCCEEEEC
PYPSSQRRVAILRGVYAMPFDSHALASDTVNRKAVSMLEDMGVVQTGDRILITKGNYADA
CCCCCCCEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHCCCEECCCEEEEEECCCCCC
HGGTNTLKIVEVGSNIQ
CCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]