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The map label for this gene is rpoH [H]

Identifier: 110835430

GI number: 110835430

Start: 2911252

End: 2912121

Strand: Direct

Name: rpoH [H]

Synonym: ABO_2569

Alternate gene names: 110835430

Gene position: 2911252-2912121 (Clockwise)

Preceding gene: 110835429

Following gene: 110835431

Centisome position: 93.31

GC content: 57.13

Gene sequence:

>870_bases
TTGCGCATGACCAACCCAACAACTCAAGCACAGGCGCTCACACTAGCCGTACCAGGCCATGACCTGGACGCCTACATGCG
AGCCGTTAACGCCGTGCCGGTACTCAGCGCAGAAGAAGAGCAACGCTTGGGCGAACGCCTGTATTACGATCAGGACTTGG
ACGCTGCGCGTAGCTTGGTTCTGTCCCACCTACGCTTTGTGGCACACATTGCCCGCAGCTACACCGGTTACGGCCTGCCA
TTAGGTGACCTGGTTCAGGAAGGCAACGTAGGCCTGATGAAAGCCGTAAAACGCTTTGACCCCACCAAGGGCGTGCGGCT
AGTGAGCTTTGCCGTACACTGGATCAAAGCAGAAATTCACGAATATGTGATCAAGAACTGGCGCATCGTGAAAGTGGCGA
CCACCAAGGCTCAGCGCAAATTGTTCTTTAACCTGCGCGGCCAGAAAAAACGCTTGGGCCGCCTGACGTTGGAAGAGGCC
CAGCGCGTGGCCGATGATTTGGGCGTTACTGCCGCACAGGTCAAAGAAATGGAAGGGCGTTTGGGCGCTTACGATGCCAG
TTTCGACGGCCCCACCAATGATGATGATGACAGCACCGTAGTGTCTCCCGCCGCTTATCTGCACAGCGATAACAGCGACC
CTGCTGACCTGCTCGCCGAACAGGATCAACAGCAGCGGGAGAGCCGTCAATTGAGCTCTGCTCTAATGGCGCTGGACGAA
CGTAGCCGCGACATTCTGGAGCGCCGCTGGCTGGCCGACCAGAAATCCACGCTGCAAGAGCTGGCCGATGAGTACAGTGT
TTCCGCCGAGCGGATTCGCCAACTGGAAAACAACGCCATCAAAAAGCTGCGTAACGCCATCGCAGCGTAA

Upstream 100 bases:

>100_bases
ATATTGATGAAAAGGAACTTTTTGGCCTATTAGCACTCTATTAATTGGAGTGCTAATATCAGCTCTCAATTGATACATAG
AGAGTCTTCACAAGGAGACA

Downstream 100 bases:

>100_bases
TGCCAGGTGTCTCACGCGGCACGCAACAGAGAATACAAAAAAGCCCCTCCCGAGGGGCTTTTTTGTAGGCCCAAGCGGCA
GCCTGTCGCTCGAGGGCAAA

Product: RNA polymerase sigma-32 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MRMTNPTTQAQALTLAVPGHDLDAYMRAVNAVPVLSAEEEQRLGERLYYDQDLDAARSLVLSHLRFVAHIARSYTGYGLP
LGDLVQEGNVGLMKAVKRFDPTKGVRLVSFAVHWIKAEIHEYVIKNWRIVKVATTKAQRKLFFNLRGQKKRLGRLTLEEA
QRVADDLGVTAAQVKEMEGRLGAYDASFDGPTNDDDDSTVVSPAAYLHSDNSDPADLLAEQDQQQRESRQLSSALMALDE
RSRDILERRWLADQKSTLQELADEYSVSAERIRQLENNAIKKLRNAIAA

Sequences:

>Translated_289_residues
MRMTNPTTQAQALTLAVPGHDLDAYMRAVNAVPVLSAEEEQRLGERLYYDQDLDAARSLVLSHLRFVAHIARSYTGYGLP
LGDLVQEGNVGLMKAVKRFDPTKGVRLVSFAVHWIKAEIHEYVIKNWRIVKVATTKAQRKLFFNLRGQKKRLGRLTLEEA
QRVADDLGVTAAQVKEMEGRLGAYDASFDGPTNDDDDSTVVSPAAYLHSDNSDPADLLAEQDQQQRESRQLSSALMALDE
RSRDILERRWLADQKSTLQELADEYSVSAERIRQLENNAIKKLRNAIAA
>Mature_289_residues
MRMTNPTTQAQALTLAVPGHDLDAYMRAVNAVPVLSAEEEQRLGERLYYDQDLDAARSLVLSHLRFVAHIARSYTGYGLP
LGDLVQEGNVGLMKAVKRFDPTKGVRLVSFAVHWIKAEIHEYVIKNWRIVKVATTKAQRKLFFNLRGQKKRLGRLTLEEA
QRVADDLGVTAAQVKEMEGRLGAYDASFDGPTNDDDDSTVVSPAAYLHSDNSDPADLLAEQDQQQRESRQLSSALMALDE
RSRDILERRWLADQKSTLQELADEYSVSAERIRQLENNAIKKLRNAIAA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=284, Percent_Identity=60.5633802816901, Blast_Score=335, Evalue=3e-93,
Organism=Escherichia coli, GI1789098, Length=289, Percent_Identity=28.3737024221453, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1789448, Length=241, Percent_Identity=28.2157676348548, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32463; Mature: 32463

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA