BacMap

Definition	Alcanivorax borkumensis SK2 chromosome, complete genome.
Accession	NC_008260
Length	3,120,143

Click here to switch to the map view.

The map label for this gene is braF [H]

Identifier: 110835373

GI number: 110835373

Start: 2849348

End: 2850193

Strand: Direct

Name: braF [H]

Synonym: ABO_2512

Alternate gene names: 110835373

Gene position: 2849348-2850193 (Clockwise)

Preceding gene: 110835372

Following gene: 110835374

Centisome position: 91.32

GC content: 54.49

Gene sequence:

>846_bases
ATGAATGCACTGGATAACCTGCGCGAGACTTTTCGCCGTGATCAGGTATTCGATTTCATGCGCCAAGGCACCCGCCCAGT
GCTCCCAGGGCAACTGGATTCTGGTACCAGTAAAAATATTCTCTACATGGAAGATATCACCGTCAGCTTTGATGGTTTCA
AAGCGATCAATGATTTGACTCTGTATATCAAAACCGGCGAATTACGCTGCATCATCGGCCCCAACGGGGCCGGTAAATCC
ACCATGATGGATGTGATCACCGGCAAGACCCGTCCGGATAGCGGCACCTGTTTTTTCGGCCAGACCCTGGACCTCACCCG
CATGAGTGAAACGGACATTGCCACCGCCGGCATCGGCCGAAAATTCCAGAAGCCCACCGTGTTCCCGGAACACACCGCTT
TCGAGAACCTGGAGCTGGCCATGCACAGCCCCAAGGGCCCCTGGCATTCTCTTTTCACTCGGCTCAGCGGCGAGCAAACA
AACTGGATTGAAGAAACGCTATACACCGTCGGGCTGTTAGGCGAGCGCAACAAGGTGGCTGGGCTGCTGTCTCATGGCCA
GAAACAATGGCTGGAAATCGGCATGGTACTGATGCAAAAACCGCAGCTGCTACTGGTGGACGAACCCATCGCCGGCATGA
CCCGCCAAGAAGTCGAGCGCACCGGAGAGTTGCTGAAAAGCCTTGCCGGCAAACATTCCGTCGTTGTCGTCGAGCATGAC
ATGGAGTTTATTCGTTCCATCGCCAGCACCGTCACGGTACTTCACCAGGGCAGTGTGCTGGCCGAAGGCACCATGGAGCA
AGTACAGAACGATCCGAAAGTGATTGAAGTGTATTTGGGCGAATAA

Upstream 100 bases:

>100_bases
TAGTGGGTTTGGTATCGGAAAATTGGGAAAAAATAAAAGCGCGATTGAATCGAAACAACAAACCATCCGGCTCCGCCAGA
CCTGCAGAAGGAGACCTGTC

Downstream 100 bases:

>100_bases
ATTCGCAGCTACACACTGCCACATTAGACAGAGACAGGAATATTTTTTGATTGCCGGTTTTTTAGACTTTTTCCCTATGC
ACGGCGAGAAGTCTGGCCGG

Product: branched-chain amino acid ABC transporter ATP-binding protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MNALDNLRETFRRDQVFDFMRQGTRPVLPGQLDSGTSKNILYMEDITVSFDGFKAINDLTLYIKTGELRCIIGPNGAGKS
TMMDVITGKTRPDSGTCFFGQTLDLTRMSETDIATAGIGRKFQKPTVFPEHTAFENLELAMHSPKGPWHSLFTRLSGEQT
NWIEETLYTVGLLGERNKVAGLLSHGQKQWLEIGMVLMQKPQLLLVDEPIAGMTRQEVERTGELLKSLAGKHSVVVVEHD
MEFIRSIASTVTVLHQGSVLAEGTMEQVQNDPKVIEVYLGE

Sequences:

>Translated_281_residues
MNALDNLRETFRRDQVFDFMRQGTRPVLPGQLDSGTSKNILYMEDITVSFDGFKAINDLTLYIKTGELRCIIGPNGAGKS
TMMDVITGKTRPDSGTCFFGQTLDLTRMSETDIATAGIGRKFQKPTVFPEHTAFENLELAMHSPKGPWHSLFTRLSGEQT
NWIEETLYTVGLLGERNKVAGLLSHGQKQWLEIGMVLMQKPQLLLVDEPIAGMTRQEVERTGELLKSLAGKHSVVVVEHD
MEFIRSIASTVTVLHQGSVLAEGTMEQVQNDPKVIEVYLGE
>Mature_281_residues
MNALDNLRETFRRDQVFDFMRQGTRPVLPGQLDSGTSKNILYMEDITVSFDGFKAINDLTLYIKTGELRCIIGPNGAGKS
TMMDVITGKTRPDSGTCFFGQTLDLTRMSETDIATAGIGRKFQKPTVFPEHTAFENLELAMHSPKGPWHSLFTRLSGEQT
NWIEETLYTVGLLGERNKVAGLLSHGQKQWLEIGMVLMQKPQLLLVDEPIAGMTRQEVERTGELLKSLAGKHSVVVVEHD
MEFIRSIASTVTVLHQGSVLAEGTMEQVQNDPKVIEVYLGE

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4674

COG function: function code R; Uncharacterized ABC-type transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI171184400, Length=228, Percent_Identity=27.1929824561404, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI105990541, Length=244, Percent_Identity=26.2295081967213, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI27881501, Length=221, Percent_Identity=23.0769230769231, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI30795238, Length=221, Percent_Identity=23.0769230769231, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI27262624, Length=218, Percent_Identity=24.7706422018349, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI27262626, Length=218, Percent_Identity=24.7706422018349, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI45446740, Length=254, Percent_Identity=24.8031496062992, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI47078218, Length=254, Percent_Identity=24.8031496062992, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI116734710, Length=215, Percent_Identity=24.1860465116279, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1789864, Length=254, Percent_Identity=34.251968503937, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1790525, Length=242, Percent_Identity=29.3388429752066, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788897, Length=227, Percent_Identity=30.8370044052863, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788225, Length=243, Percent_Identity=28.8065843621399, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1786398, Length=226, Percent_Identity=28.3185840707965, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1787370, Length=240, Percent_Identity=29.1666666666667, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1790544, Length=242, Percent_Identity=26.4462809917355, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1790190, Length=247, Percent_Identity=29.1497975708502, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790739, Length=237, Percent_Identity=29.535864978903, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87081791, Length=235, Percent_Identity=25.9574468085106, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788761, Length=247, Percent_Identity=27.5303643724696, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87081709, Length=241, Percent_Identity=25.7261410788382, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788210, Length=239, Percent_Identity=28.0334728033473, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1787792, Length=233, Percent_Identity=27.0386266094421, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790467, Length=228, Percent_Identity=28.5087719298246, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1789593, Length=246, Percent_Identity=25.609756097561, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI87082268, Length=244, Percent_Identity=27.0491803278689, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788472, Length=247, Percent_Identity=27.9352226720648, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787089, Length=228, Percent_Identity=29.3859649122807, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1789991, Length=226, Percent_Identity=26.9911504424779, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787029, Length=239, Percent_Identity=26.3598326359833, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI48995001, Length=226, Percent_Identity=27.8761061946903, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786872, Length=243, Percent_Identity=26.3374485596708, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1789586, Length=244, Percent_Identity=24.5901639344262, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790162, Length=239, Percent_Identity=25.5230125523013, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787182, Length=240, Percent_Identity=25, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788506, Length=256, Percent_Identity=25.390625, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081834, Length=211, Percent_Identity=25.5924170616114, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786803, Length=237, Percent_Identity=25.7383966244726, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787164, Length=235, Percent_Identity=25.9574468085106, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87082267, Length=240, Percent_Identity=25.8333333333333, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786319, Length=219, Percent_Identity=26.027397260274, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789672, Length=238, Percent_Identity=22.6890756302521, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787500, Length=254, Percent_Identity=25.9842519685039, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789032, Length=229, Percent_Identity=25.764192139738, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI48994943, Length=232, Percent_Identity=26.7241379310345, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786703, Length=187, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787547, Length=225, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786345, Length=246, Percent_Identity=23.1707317073171, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789873, Length=160, Percent_Identity=28.75, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788644, Length=254, Percent_Identity=27.1653543307087, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787712, Length=221, Percent_Identity=23.9819004524887, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789962, Length=227, Percent_Identity=25.5506607929515, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787972, Length=238, Percent_Identity=26.890756302521, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87081782, Length=231, Percent_Identity=24.6753246753247, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787105, Length=248, Percent_Identity=26.2096774193548, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786654, Length=242, Percent_Identity=26.4462809917355, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788002, Length=225, Percent_Identity=24, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786253, Length=244, Percent_Identity=24.1803278688525, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787548, Length=252, Percent_Identity=21.8253968253968, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71983523, Length=241, Percent_Identity=26.9709543568465, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71983510, Length=241, Percent_Identity=26.9709543568465, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71983516, Length=241, Percent_Identity=26.9709543568465, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI115533608, Length=210, Percent_Identity=27.6190476190476, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17510237, Length=220, Percent_Identity=26.8181818181818, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17565586, Length=217, Percent_Identity=24.4239631336406, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28574150, Length=210, Percent_Identity=26.6666666666667, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI116007328, Length=210, Percent_Identity=26.6666666666667, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI116007184, Length=215, Percent_Identity=21.8604651162791, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI221500365, Length=215, Percent_Identity=21.8604651162791, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28574533, Length=206, Percent_Identity=27.6699029126214, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 31303; Mature: 31303

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALDNLRETFRRDQVFDFMRQGTRPVLPGQLDSGTSKNILYMEDITVSFDGFKAINDLT
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCEEECEEE
LYIKTGELRCIIGPNGAGKSTMMDVITGKTRPDSGTCFFGQTLDLTRMSETDIATAGIGR
EEEEECCEEEEECCCCCCHHHHHHHHCCCCCCCCCCEEECCCCCCEECCCCCHHHHCCCC
KFQKPTVFPEHTAFENLELAMHSPKGPWHSLFTRLSGEQTNWIEETLYTVGLLGERNKVA
CCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHH
GLLSHGQKQWLEIGMVLMQKPQLLLVDEPIAGMTRQEVERTGELLKSLAGKHSVVVVEHD
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECC
MEFIRSIASTVTVLHQGSVLAEGTMEQVQNDPKVIEVYLGE
HHHHHHHHHHHHHHHCCCEEECCHHHHHCCCCEEEEEEECC
>Mature Secondary Structure
MNALDNLRETFRRDQVFDFMRQGTRPVLPGQLDSGTSKNILYMEDITVSFDGFKAINDLT
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCEEECEEE
LYIKTGELRCIIGPNGAGKSTMMDVITGKTRPDSGTCFFGQTLDLTRMSETDIATAGIGR
EEEEECCEEEEECCCCCCHHHHHHHHCCCCCCCCCCEEECCCCCCEECCCCCHHHHCCCC
KFQKPTVFPEHTAFENLELAMHSPKGPWHSLFTRLSGEQTNWIEETLYTVGLLGERNKVA
CCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHH
GLLSHGQKQWLEIGMVLMQKPQLLLVDEPIAGMTRQEVERTGELLKSLAGKHSVVVVEHD
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECC
MEFIRSIASTVTVLHQGSVLAEGTMEQVQNDPKVIEVYLGE
HHHHHHHHHHHHHHHCCCEEECCHHHHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]