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The map label for this gene is hisH [H]

Identifier: 110835141

GI number: 110835141

Start: 2599559

End: 2600200

Strand: Reverse

Name: hisH [H]

Synonym: ABO_2280

Alternate gene names: 110835141

Gene position: 2600200-2599559 (Counterclockwise)

Preceding gene: 110835142

Following gene: 110835140

Centisome position: 83.34

GC content: 57.32

Gene sequence:

>642_bases
ATGAGCGAAGTCGTTGCCGTTATTGATTACGGAATGGGTAACCTGCACTCCGCAGGTAAGGCGCTGGAGAAAGTCGCCGA
TGGCCAGAAAATCATTATTACCGGCGATCCGGCGCAGGTGCGTGCAGCGGACCGGGTGGTTTTTCCTGGTGTGGGTGCTA
TTCGTGACTGCATCGCGGTTCTGAAAGACACCGGCCTGGATCAGGCCATCCGTGATGTGGCGGCGGCGGGCAAACCGCTG
TTGGGCATCTGTGTCGGGATGCAGGCAATGATGGCCCGTAGTGAAGAAAATCACGGGGTGGATTGCCTGGGCATCTTCGA
CGGCCAGGTGACTTTTTTCGGTGAGCAGTTCAGCGATACCGGTGTTCGCTTGAAAGTGCCGCACATGGGCTGGAACCAGG
TACAGCAGTGCATGGCCCATCCTATGTGGGCCGGTATCGACAACCATACCCGGTTTTATTTTGTGCACAGTTACTGCGTT
ACCGGCCTGCCCGATGACGCTGTGGCAGGGCGCTGTGATTATGGTTTGAGTTTTGCGGCAGCCGCGGTTCAAGGCAACGT
GTTTGCGGTGCAGTTCCACCCGGAAAAAAGCGCGGATGCAGGCCTCGCCCTGTTGGAAAACTTCCTGCGCTGGCAGCCAT
AA

Upstream 100 bases:

>100_bases
CTCGTATGGATGGTATTACGCCCTCCACGAAGGGCACCCTGAGCGAAAGTGGCGATAGCCAGTAAGCAGCCCAGTAGCAA
TTACAAGGACAGCCCCGAGT

Downstream 100 bases:

>100_bases
AAGACAGCAGGCTTGCACGCTAGGGTTTCTCTCTACGGTGTTCGTATAACGGGCGCGGCGGGGGAGGGATGCCGATTTCC
ATGAATTTAAATGAAGAGAT

Product: glutamine amidotransferase subunit hisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit 1; IGP synthase subunit hisH 1; ImGP synthase subunit hisH 1; IGPS subunit hisH 1 [H]

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MSEVVAVIDYGMGNLHSAGKALEKVADGQKIIITGDPAQVRAADRVVFPGVGAIRDCIAVLKDTGLDQAIRDVAAAGKPL
LGICVGMQAMMARSEENHGVDCLGIFDGQVTFFGEQFSDTGVRLKVPHMGWNQVQQCMAHPMWAGIDNHTRFYFVHSYCV
TGLPDDAVAGRCDYGLSFAAAAVQGNVFAVQFHPEKSADAGLALLENFLRWQP

Sequences:

>Translated_213_residues
MSEVVAVIDYGMGNLHSAGKALEKVADGQKIIITGDPAQVRAADRVVFPGVGAIRDCIAVLKDTGLDQAIRDVAAAGKPL
LGICVGMQAMMARSEENHGVDCLGIFDGQVTFFGEQFSDTGVRLKVPHMGWNQVQQCMAHPMWAGIDNHTRFYFVHSYCV
TGLPDDAVAGRCDYGLSFAAAAVQGNVFAVQFHPEKSADAGLALLENFLRWQP
>Mature_212_residues
SEVVAVIDYGMGNLHSAGKALEKVADGQKIIITGDPAQVRAADRVVFPGVGAIRDCIAVLKDTGLDQAIRDVAAAGKPLL
GICVGMQAMMARSEENHGVDCLGIFDGQVTFFGEQFSDTGVRLKVPHMGWNQVQQCMAHPMWAGIDNHTRFYFVHSYCVT
GLPDDAVAGRCDYGLSFAAAAVQGNVFAVQFHPEKSADAGLALLENFLRWQP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=206, Percent_Identity=39.3203883495146, Blast_Score=139, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6319725, Length=219, Percent_Identity=34.703196347032, Blast_Score=125, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22868; Mature: 22737

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVVAVIDYGMGNLHSAGKALEKVADGQKIIITGDPAQVRAADRVVFPGVGAIRDCIAV
CCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHCCEEECCCCHHHHHHHHH
LKDTGLDQAIRDVAAAGKPLLGICVGMQAMMARSEENHGVDCLGIFDGQVTFFGEQFSDT
HHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEECCCCCCC
GVRLKVPHMGWNQVQQCMAHPMWAGIDNHTRFYFVHSYCVTGLPDDAVAGRCDYGLSFAA
CCEEEECCCCHHHHHHHHCCCCCCCCCCCEEEEEEEEEEECCCCCCHHCCCCCCCHHHHH
AAVQGNVFAVQFHPEKSADAGLALLENFLRWQP
HHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEVVAVIDYGMGNLHSAGKALEKVADGQKIIITGDPAQVRAADRVVFPGVGAIRDCIAV
CCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHCCEEECCCCHHHHHHHHH
LKDTGLDQAIRDVAAAGKPLLGICVGMQAMMARSEENHGVDCLGIFDGQVTFFGEQFSDT
HHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEECCCCCCC
GVRLKVPHMGWNQVQQCMAHPMWAGIDNHTRFYFVHSYCVTGLPDDAVAGRCDYGLSFAA
CCEEEECCCCHHHHHHHHCCCCCCCCCCCEEEEEEEEEEECCCCCCHHCCCCCCCHHHHH
AAVQGNVFAVQFHPEKSADAGLALLENFLRWQP
HHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]