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The map label for this gene is hisF

Identifier: 110835139

GI number: 110835139

Start: 2597921

End: 2598697

Strand: Reverse

Name: hisF

Synonym: ABO_2278

Alternate gene names: 110835139

Gene position: 2598697-2597921 (Counterclockwise)

Preceding gene: 110835140

Following gene: 110835135

Centisome position: 83.29

GC content: 57.27

Gene sequence:

>777_bases
ATGGGTTTAGCCAAACGCATTATTCCGTGTCTGGATGTGGACGCCGGCCGAGTGGTCAAGGGCGTGCAATTCGTGGATAT
CCGTGACGCCGGTGACCCGGTGGAAATTGCCAAGCGCTATAACGACGCTGGGGCGGATGAAATTACCTTTTTGGACATCA
CCGCCAGCCATCAAGAGCGCGATACCACCCTGCACACGGTGGAACAGATCGCCAGCCAGGTGTTTATCCCGCTGACCGTG
GGTGGCGGTGTGCGTGCGTTGGAGGATATTCGTAACTTGCTGAATGCCGGTGCCGACAAGGTGGCGATCAACTCTGCCGC
GGTGCATAACCCGGAGTTTGTTCGTGAGGCGGCGGAGCGGTTTGGTTCCCAATGTATTGTGGTGGCGATTGATGCGAAAA
AAGTCAGCGCGGAAAGCGATACCAACCGCTGGGAAATCTTCACCCACGGCGGTCGTAAACCCACTGGGCTGGATGCGGTG
GAATGGGCCCGGAAAATGACCGACTACGGCGCGGGTGAAATTCTGTTGACCAGCATGGACCGCGATGGCACCAAGAATGG
TTTTGATATTGCCCTGACTCGAGCCATTGCCGATGCGGTCCCAGTCCCGGTGATTGCCTCTGGCGGGGTTGGCAACCTAC
AGCACTTGGTTGATGGTGTGCAGCAGGGCGGTGCTGATGCGGTGTTGGCGGCGTCGATTTTCCATTTCGGAGAGTATTCA
GTGCAGCAAGCCAAGGAGTTTATGCAGTCTGCCGGCGTGGAAATGCGCCTAGGCTAA

Upstream 100 bases:

>100_bases
GCTATCACGGGCCGTGCCATCTATGAGAACACCCTGGACTTTGCCGATGGTCAGGCGTTGTCCGATGAGTTGAGCGGCGC
CTGAGCGCCGGGGACGTATT

Downstream 100 bases:

>100_bases
CCGGTGTTTTCTTCTTGTCGGGCGGATTACACCCTTCCTGTCATCACTTTAGAGCGTTGCGTACTGGTCATAAATTAGCC
CCACTTTTCTCTTGGCTTGG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MGLAKRIIPCLDVDAGRVVKGVQFVDIRDAGDPVEIAKRYNDAGADEITFLDITASHQERDTTLHTVEQIASQVFIPLTV
GGGVRALEDIRNLLNAGADKVAINSAAVHNPEFVREAAERFGSQCIVVAIDAKKVSAESDTNRWEIFTHGGRKPTGLDAV
EWARKMTDYGAGEILLTSMDRDGTKNGFDIALTRAIADAVPVPVIASGGVGNLQHLVDGVQQGGADAVLAASIFHFGEYS
VQQAKEFMQSAGVEMRLG

Sequences:

>Translated_258_residues
MGLAKRIIPCLDVDAGRVVKGVQFVDIRDAGDPVEIAKRYNDAGADEITFLDITASHQERDTTLHTVEQIASQVFIPLTV
GGGVRALEDIRNLLNAGADKVAINSAAVHNPEFVREAAERFGSQCIVVAIDAKKVSAESDTNRWEIFTHGGRKPTGLDAV
EWARKMTDYGAGEILLTSMDRDGTKNGFDIALTRAIADAVPVPVIASGGVGNLQHLVDGVQQGGADAVLAASIFHFGEYS
VQQAKEFMQSAGVEMRLG
>Mature_257_residues
GLAKRIIPCLDVDAGRVVKGVQFVDIRDAGDPVEIAKRYNDAGADEITFLDITASHQERDTTLHTVEQIASQVFIPLTVG
GGVRALEDIRNLLNAGADKVAINSAAVHNPEFVREAAERFGSQCIVVAIDAKKVSAESDTNRWEIFTHGGRKPTGLDAVE
WARKMTDYGAGEILLTSMDRDGTKNGFDIALTRAIADAVPVPVIASGGVGNLQHLVDGVQQGGADAVLAASIFHFGEYSV
QQAKEFMQSAGVEMRLG

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=259, Percent_Identity=40.5405405405405, Blast_Score=205, Evalue=3e-54,
Organism=Escherichia coli, GI87082028, Length=249, Percent_Identity=28.9156626506024, Blast_Score=97, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319725, Length=316, Percent_Identity=34.8101265822785, Blast_Score=166, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_ALCBS (Q0VM72)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_693998.1
- ProteinModelPortal:   Q0VM72
- SMR:   Q0VM72
- STRING:   Q0VM72
- GeneID:   4212202
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_2278
- NMPDR:   fig|393595.12.peg.2280
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   Q0VM72
- ProtClustDB:   PRK02083
- BioCyc:   ABOR393595:ABO_2278-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27543; Mature: 27412

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: ACT_SITE 12-12 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLAKRIIPCLDVDAGRVVKGVQFVDIRDAGDPVEIAKRYNDAGADEITFLDITASHQER
CCCHHHHHHHCCCCCCHHHCCEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEEECCCCHH
DTTLHTVEQIASQVFIPLTVGGGVRALEDIRNLLNAGADKVAINSAAVHNPEFVREAAER
HHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCCEEECCHHCCCHHHHHHHHHH
FGSQCIVVAIDAKKVSAESDTNRWEIFTHGGRKPTGLDAVEWARKMTDYGAGEILLTSMD
HCCCEEEEEEECCEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECC
RDGTKNGFDIALTRAIADAVPVPVIASGGVGNLQHLVDGVQQGGADAVLAASIFHFGEYS
CCCCCCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCHH
VQQAKEFMQSAGVEMRLG
HHHHHHHHHHCCCEEECC
>Mature Secondary Structure 
GLAKRIIPCLDVDAGRVVKGVQFVDIRDAGDPVEIAKRYNDAGADEITFLDITASHQER
CCHHHHHHHCCCCCCHHHCCEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEEECCCCHH
DTTLHTVEQIASQVFIPLTVGGGVRALEDIRNLLNAGADKVAINSAAVHNPEFVREAAER
HHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCCEEECCHHCCCHHHHHHHHHH
FGSQCIVVAIDAKKVSAESDTNRWEIFTHGGRKPTGLDAVEWARKMTDYGAGEILLTSMD
HCCCEEEEEEECCEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECC
RDGTKNGFDIALTRAIADAVPVPVIASGGVGNLQHLVDGVQQGGADAVLAASIFHFGEYS
CCCCCCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCHH
VQQAKEFMQSAGVEMRLG
HHHHHHHHHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA