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The map label for this gene is radA [H]

Identifier: 110835046

GI number: 110835046

Start: 2494368

End: 2495741

Strand: Reverse

Name: radA [H]

Synonym: ABO_2185

Alternate gene names: 110835046

Gene position: 2495741-2494368 (Counterclockwise)

Preceding gene: 110835047

Following gene: 110835037

Centisome position: 79.99

GC content: 56.55

Gene sequence:

>1374_bases
TTGTTTGTGGCCAAGAAAAAGATCGCGTTTGTATGCACGGATTGCGGAGCGGATTTTCCCAAATGGCAGGGTCAGTGTCC
GGCCTGTAATGCCTGGAATACCTTGCAAGAGTTTGTCCACGATCCTGCTACCCCATCATCAAAAGGGGCGGGCAGCCGGG
GCGGCTTTTCCGGCCAGCTGTCAGAAGTGCAAAATCTCAATGATATTGTGCTCGAAGAAACCCCGCGTATCAGTTCCGGC
ATGGGCGAGCTGGATCGAGTGCTGGGTGGCGGGTTGGTGCCTGGTTCGGCCATTCTGATTGGTGGGCACCCCGGTGCGGG
GAAATCTACCTTGCTACTACAAACGCTGTGTAGGCTCTCTGACTCCCAAAGCTGTTTGTATATCACCGGCGAAGAATCCT
TGTCGCAAGTGGCTATGCGTGCTGACCGCCTGAAGCTACCCAAGGAAAAACTGCGACTGGCGGCGGAGACTGACGTGGAG
CAGATTCTCGAATTGGCCCGCAAGGAGATGCCGAAGGTGTTGGTGGTGGATTCTATCCAGGTCATGTTTCTGGCGGCGTT
GCAGTCGGCGCCGGGCAGTGTGGCTCAGGTTCGTGAATGTGCTGCCGCTCTGACTCGCTTTGCCAAGCAGACGGGAACAG
TGCTGCTGCTGGTGGGCCATGTAACCAAGGATGGCACCCTGGCGGGGCCGAAGGTATTGGAGCACATGATTGATTGTTCC
CTGATGCTGGAAGGATCTACGGATTCGCGCTTTCGTACCCTGCGTGGCCTCAAGAATCGTTTTGGGGCGGTGAACGAGCT
GGGCGTATTTGCCATGACCGGCGAGGGGCTGAAGGAAGTAAAAAATCCTTCCGCCATTTTCCTTAACCGGGCCGAGGAGA
TCGCTTCCGGTTCTCTGGTAACGGTGGTCTGGGAAGGAACCCGTCCGCTGCTGGTGGAGCTGCAGGCACTGGTGGACGCC
TCCCACTTCGGTAACCCTCGGCGTATCTGTGTGGGGTTGGAGGGGAATCGGTTGGCCATGTTGCTGGCGGTACTGCATCG
CCATGGTGGCATTCAGGTAGGCGATCAGGATGTGTTCATGAATGTGGTGGGTGGTGTGAAGGTTACCGAAACGGCTGCAG
ACTTGGCCCAATTGCTGGCTATTGTGTCCAGCTTCCGTGACCGGGCGCTGGAGCGCGAGCTGGTGGTGTTTGGTGAGGTG
GGCTTGTCCGGGGAAATTCGGCCGGTTCCTCAAGGGCAGGAACGGCTTTATGAAGCAGTAAAACACGGCTTTAAAAAAGC
CATTGTGCCGAAAGCCAACTTGCCTAGAAAGCTGCCAGACGGGGTGGACATTATTGGCGTTAGTAACGTGGCTGAGGCGC
TGGAATATCTGTAA

Upstream 100 bases:

>100_bases
TTATTCGCTGCGCTCACCCCTTTGGGGCCGCACTCCGTGCGTTACTCCGCTGCGCTACGTTCCTGCAGCGGCTTTGTAGA
TAGCAATACACAAGAAGGAT

Downstream 100 bases:

>100_bases
TTAGTTGAGAGTGGAAGAGTTAACCGCTCGCGAATCGATTTTTTATTTTAAAGGGTGGAGCCACGTCGAGAGACAGAGCG
CGCTCGCGCTGCCGCTCTAT

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MFVAKKKIAFVCTDCGADFPKWQGQCPACNAWNTLQEFVHDPATPSSKGAGSRGGFSGQLSEVQNLNDIVLEETPRISSG
MGELDRVLGGGLVPGSAILIGGHPGAGKSTLLLQTLCRLSDSQSCLYITGEESLSQVAMRADRLKLPKEKLRLAAETDVE
QILELARKEMPKVLVVDSIQVMFLAALQSAPGSVAQVRECAAALTRFAKQTGTVLLLVGHVTKDGTLAGPKVLEHMIDCS
LMLEGSTDSRFRTLRGLKNRFGAVNELGVFAMTGEGLKEVKNPSAIFLNRAEEIASGSLVTVVWEGTRPLLVELQALVDA
SHFGNPRRICVGLEGNRLAMLLAVLHRHGGIQVGDQDVFMNVVGGVKVTETAADLAQLLAIVSSFRDRALERELVVFGEV
GLSGEIRPVPQGQERLYEAVKHGFKKAIVPKANLPRKLPDGVDIIGVSNVAEALEYL

Sequences:

>Translated_457_residues
MFVAKKKIAFVCTDCGADFPKWQGQCPACNAWNTLQEFVHDPATPSSKGAGSRGGFSGQLSEVQNLNDIVLEETPRISSG
MGELDRVLGGGLVPGSAILIGGHPGAGKSTLLLQTLCRLSDSQSCLYITGEESLSQVAMRADRLKLPKEKLRLAAETDVE
QILELARKEMPKVLVVDSIQVMFLAALQSAPGSVAQVRECAAALTRFAKQTGTVLLLVGHVTKDGTLAGPKVLEHMIDCS
LMLEGSTDSRFRTLRGLKNRFGAVNELGVFAMTGEGLKEVKNPSAIFLNRAEEIASGSLVTVVWEGTRPLLVELQALVDA
SHFGNPRRICVGLEGNRLAMLLAVLHRHGGIQVGDQDVFMNVVGGVKVTETAADLAQLLAIVSSFRDRALERELVVFGEV
GLSGEIRPVPQGQERLYEAVKHGFKKAIVPKANLPRKLPDGVDIIGVSNVAEALEYL
>Mature_457_residues
MFVAKKKIAFVCTDCGADFPKWQGQCPACNAWNTLQEFVHDPATPSSKGAGSRGGFSGQLSEVQNLNDIVLEETPRISSG
MGELDRVLGGGLVPGSAILIGGHPGAGKSTLLLQTLCRLSDSQSCLYITGEESLSQVAMRADRLKLPKEKLRLAAETDVE
QILELARKEMPKVLVVDSIQVMFLAALQSAPGSVAQVRECAAALTRFAKQTGTVLLLVGHVTKDGTLAGPKVLEHMIDCS
LMLEGSTDSRFRTLRGLKNRFGAVNELGVFAMTGEGLKEVKNPSAIFLNRAEEIASGSLVTVVWEGTRPLLVELQALVDA
SHFGNPRRICVGLEGNRLAMLLAVLHRHGGIQVGDQDVFMNVVGGVKVTETAADLAQLLAIVSSFRDRALERELVVFGEV
GLSGEIRPVPQGQERLYEAVKHGFKKAIVPKANLPRKLPDGVDIIGVSNVAEALEYL

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=450, Percent_Identity=67.5555555555556, Blast_Score=624, Evalue=1e-180,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 48980; Mature: 48980

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVAKKKIAFVCTDCGADFPKWQGQCPACNAWNTLQEFVHDPATPSSKGAGSRGGFSGQL
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
SEVQNLNDIVLEETPRISSGMGELDRVLGGGLVPGSAILIGGHPGAGKSTLLLQTLCRLS
HHHCCCHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCC
DSQSCLYITGEESLSQVAMRADRLKLPKEKLRLAAETDVEQILELARKEMPKVLVVDSIQ
CCCCEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHCCCEEEEHHHH
VMFLAALQSAPGSVAQVRECAAALTRFAKQTGTVLLLVGHVTKDGTLAGPKVLEHMIDCS
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHCCE
LMLEGSTDSRFRTLRGLKNRFGAVNELGVFAMTGEGLKEVKNPSAIFLNRAEEIASGSLV
EEEECCCCHHHHHHHHHHHHCCCHHHCCEEEECCCHHHHHCCCCCEEEECHHHHCCCCEE
TVVWEGTRPLLVELQALVDASHFGNPRRICVGLEGNRLAMLLAVLHRHGGIQVGDQDVFM
EEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCEECCHHHHH
NVVGGVKVTETAADLAQLLAIVSSFRDRALERELVVFGEVGLSGEIRPVPQGQERLYEAV
HHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHH
KHGFKKAIVPKANLPRKLPDGVDIIGVSNVAEALEYL
HHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHCC
>Mature Secondary Structure
MFVAKKKIAFVCTDCGADFPKWQGQCPACNAWNTLQEFVHDPATPSSKGAGSRGGFSGQL
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
SEVQNLNDIVLEETPRISSGMGELDRVLGGGLVPGSAILIGGHPGAGKSTLLLQTLCRLS
HHHCCCHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCC
DSQSCLYITGEESLSQVAMRADRLKLPKEKLRLAAETDVEQILELARKEMPKVLVVDSIQ
CCCCEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHCCCEEEEHHHH
VMFLAALQSAPGSVAQVRECAAALTRFAKQTGTVLLLVGHVTKDGTLAGPKVLEHMIDCS
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHCCE
LMLEGSTDSRFRTLRGLKNRFGAVNELGVFAMTGEGLKEVKNPSAIFLNRAEEIASGSLV
EEEECCCCHHHHHHHHHHHHCCCHHHCCEEEECCCHHHHHCCCCCEEEECHHHHCCCCEE
TVVWEGTRPLLVELQALVDASHFGNPRRICVGLEGNRLAMLLAVLHRHGGIQVGDQDVFM
EEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCEECCHHHHH
NVVGGVKVTETAADLAQLLAIVSSFRDRALERELVVFGEVGLSGEIRPVPQGQERLYEAV
HHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHH
KHGFKKAIVPKANLPRKLPDGVDIIGVSNVAEALEYL
HHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1327967; 8759876; 7610040; 9278503 [H]