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The map label for this gene is miaB

Identifier: 110834795

GI number: 110834795

Start: 2209581

End: 2210918

Strand: Direct

Name: miaB

Synonym: ABO_1934

Alternate gene names: 110834795

Gene position: 2209581-2210918 (Clockwise)

Preceding gene: 110834791

Following gene: 110834796

Centisome position: 70.82

GC content: 55.53

Gene sequence:

>1338_bases
ATGGCACGTAAGCTGTTTATCCAGACCCACGGCTGCCAGATGAACGAGTATGACTCCACTCGTATGGTGGACCTGCTCGA
ATCCAGCCACGGCCTGGAGCCCACCGACAATCCCGAAGAGGCGGATGTGCTGCTTCTCAACACCTGCTCTATCCGGGAAA
AAGCCCAGGAAAAGGTCTTCCACCAACTCGGTCGCTGGAAAAAACTGAAAGACGCCAAGCCCGATATGATTATCGGCGTG
GGCGGCTGTGTGGCCAGCCAAGAGGGCGACGCTATCCGCGACCGCGCCCCCTATGTGGACGTAGTCTTTGGCCCGCAAAC
ACTGCATCGCCTGCCCGGCCTCATTACTCAGGCCGCCAGCACCCGTGAGCTGGCCATCGATGTAACCTTCCCGGAAATTG
AAAAGTTCGACAACCTTCCAGAACCCAGTGTAGACGGCCCCTCCGCCTTTGTATCCATAATGGAGGGCTGCTCCAAATAT
TGCACCTTCTGCGTAGTGCCCTATACCCGCGGTGAAGAAGTCAGCCGTCCGGTGCAACCCGTCCTCAAAGAAATCCAGCA
CCTGGCCGACATGGGCGTTCGCGAGGTTAACTTGTTAGGCCAAAACGTGAACGCCTACCAAGGCGTGGGGGCCGACGGCG
ATACACTGGATCTGGCAGACCTGATCCGACTAATCCGGGATATCGACGGTATTGACCGCATTCGCTACACCACCAGCCAC
CCAGTGGAATTCTCTGAAGCGCTGATTCAAGTGTACGAAGACGTGCCCGAGCTGGTCAGCCACCTGCATCTGCCGGTGCA
ATCTGGCTCCGACCGTATCCTGGCGATGATGAAACGCAACCACATGGTGCTGGAATACAAGAGCAAGCTGCGCAAGCTCA
AACGCATCCGTCCGGATATTTCCTTTAGCTCGGATTTCATCATCGGCTTTCCCGGCGAAACAGACCGGGATTTCGAAGAC
ACTATGAACCTGATCCATGACATCGGCTTCGACATGTCATTCAGCTTTATCTACAGCGCCCGCCCAGGCACGCCCGCAGC
GGACCTGCCCGACGATGTGGATATGGAAGTAAAGAAGCAGCGCTTGGCCATTCTCCAGCAACGCATCAACCAGAATGTCC
AGGATATCTCCCGCAAAATGGTGGGCAGTACCCAGCGCATTCTTGTAGACGGCTTCTCCAAGAAGGACCCCGGCCAGCTA
AAAGGGCGCACCGAGAACAACCGAGTAGTTAACTTCCAGTGTGATGACACCGACCTAATTGGTAAATTCGTAGATGTGAC
CATTGCAAAGGCTTACAGCAATTCATTGCTAGGGACTGATCCGCGCAACCCGAGTTAA

Upstream 100 bases:

>100_bases
ACATACAGCAGTTTTCCCATGATTCCTCCGGTTCGCAGTTTGCCGCAGTCAGCGCTATAATCCGCGCCCATTTTCACTCT
ATTTGACGGTTAGGCAACCC

Downstream 100 bases:

>100_bases
TCCCCGGCCTTGCGCACTCATCCCATTATTTCTCTTCATTCCAACAAAGCAGTTTTTAATGGATGCTCATATAGACTAGA
GCCTTGTTCGTGCAACCCAG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MARKLFIQTHGCQMNEYDSTRMVDLLESSHGLEPTDNPEEADVLLLNTCSIREKAQEKVFHQLGRWKKLKDAKPDMIIGV
GGCVASQEGDAIRDRAPYVDVVFGPQTLHRLPGLITQAASTRELAIDVTFPEIEKFDNLPEPSVDGPSAFVSIMEGCSKY
CTFCVVPYTRGEEVSRPVQPVLKEIQHLADMGVREVNLLGQNVNAYQGVGADGDTLDLADLIRLIRDIDGIDRIRYTTSH
PVEFSEALIQVYEDVPELVSHLHLPVQSGSDRILAMMKRNHMVLEYKSKLRKLKRIRPDISFSSDFIIGFPGETDRDFED
TMNLIHDIGFDMSFSFIYSARPGTPAADLPDDVDMEVKKQRLAILQQRINQNVQDISRKMVGSTQRILVDGFSKKDPGQL
KGRTENNRVVNFQCDDTDLIGKFVDVTIAKAYSNSLLGTDPRNPS

Sequences:

>Translated_445_residues
MARKLFIQTHGCQMNEYDSTRMVDLLESSHGLEPTDNPEEADVLLLNTCSIREKAQEKVFHQLGRWKKLKDAKPDMIIGV
GGCVASQEGDAIRDRAPYVDVVFGPQTLHRLPGLITQAASTRELAIDVTFPEIEKFDNLPEPSVDGPSAFVSIMEGCSKY
CTFCVVPYTRGEEVSRPVQPVLKEIQHLADMGVREVNLLGQNVNAYQGVGADGDTLDLADLIRLIRDIDGIDRIRYTTSH
PVEFSEALIQVYEDVPELVSHLHLPVQSGSDRILAMMKRNHMVLEYKSKLRKLKRIRPDISFSSDFIIGFPGETDRDFED
TMNLIHDIGFDMSFSFIYSARPGTPAADLPDDVDMEVKKQRLAILQQRINQNVQDISRKMVGSTQRILVDGFSKKDPGQL
KGRTENNRVVNFQCDDTDLIGKFVDVTIAKAYSNSLLGTDPRNPS
>Mature_444_residues
ARKLFIQTHGCQMNEYDSTRMVDLLESSHGLEPTDNPEEADVLLLNTCSIREKAQEKVFHQLGRWKKLKDAKPDMIIGVG
GCVASQEGDAIRDRAPYVDVVFGPQTLHRLPGLITQAASTRELAIDVTFPEIEKFDNLPEPSVDGPSAFVSIMEGCSKYC
TFCVVPYTRGEEVSRPVQPVLKEIQHLADMGVREVNLLGQNVNAYQGVGADGDTLDLADLIRLIRDIDGIDRIRYTTSHP
VEFSEALIQVYEDVPELVSHLHLPVQSGSDRILAMMKRNHMVLEYKSKLRKLKRIRPDISFSSDFIIGFPGETDRDFEDT
MNLIHDIGFDMSFSFIYSARPGTPAADLPDDVDMEVKKQRLAILQQRINQNVQDISRKMVGSTQRILVDGFSKKDPGQLK
GRTENNRVVNFQCDDTDLIGKFVDVTIAKAYSNSLLGTDPRNPS

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=479, Percent_Identity=35.2818371607516, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI28872782, Length=479, Percent_Identity=35.2818371607516, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI93277076, Length=405, Percent_Identity=29.3827160493827, Blast_Score=152, Evalue=8e-37,
Organism=Escherichia coli, GI1786882, Length=438, Percent_Identity=68.2648401826484, Blast_Score=624, Evalue=1e-180,
Organism=Escherichia coli, GI1787057, Length=427, Percent_Identity=27.6346604215457, Blast_Score=148, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17553146, Length=465, Percent_Identity=32.9032258064516, Blast_Score=243, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI71996771, Length=244, Percent_Identity=29.9180327868852, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI21356207, Length=477, Percent_Identity=33.1236897274633, Blast_Score=262, Evalue=4e-70,
Organism=Drosophila melanogaster, GI19922432, Length=446, Percent_Identity=26.9058295964126, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_ALCBS (Q0VN66)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_693654.1
- ProteinModelPortal:   Q0VN66
- SMR:   Q0VN66
- STRING:   Q0VN66
- GeneID:   4211750
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_1934
- NMPDR:   fig|393595.12.peg.1935
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   Q0VN66
- ProtClustDB:   PRK14325
- BioCyc:   ABOR393595:ABO_1934-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49900; Mature: 49769

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKLFIQTHGCQMNEYDSTRMVDLLESSHGLEPTDNPEEADVLLLNTCSIREKAQEKVF
CCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHH
HQLGRWKKLKDAKPDMIIGVGGCVASQEGDAIRDRAPYVDVVFGPQTLHRLPGLITQAAS
HHHHHHHHHCCCCCCEEEECCCEECCCCCCCHHCCCCEEEEEECHHHHHHHHHHHHHCCC
TRELAIDVTFPEIEKFDNLPEPSVDGPSAFVSIMEGCSKYCTFCVVPYTRGEEVSRPVQP
CCEEEEEEECHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCCCHHCCCHHH
VLKEIQHLADMGVREVNLLGQNVNAYQGVGADGDTLDLADLIRLIRDIDGIDRIRYTTSH
HHHHHHHHHHCCCHHHHHHCCCCCHHCCCCCCCCCCCHHHHHHHHHHCCCHHHEEECCCC
PVEFSEALIQVYEDVPELVSHLHLPVQSGSDRILAMMKRNHMVLEYKSKLRKLKRIRPDI
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEHHHHHHHHHHHHHCCCC
SFSSDFIIGFPGETDRDFEDTMNLIHDIGFDMSFSFIYSARPGTPAADLPDDVDMEVKKQ
CCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHH
RLAILQQRINQNVQDISRKMVGSTQRILVDGFSKKDPGQLKGRTENNRVVNFQCDDTDLI
HHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHHH
GKFVDVTIAKAYSNSLLGTDPRNPS
HHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
ARKLFIQTHGCQMNEYDSTRMVDLLESSHGLEPTDNPEEADVLLLNTCSIREKAQEKVF
CCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHH
HQLGRWKKLKDAKPDMIIGVGGCVASQEGDAIRDRAPYVDVVFGPQTLHRLPGLITQAAS
HHHHHHHHHCCCCCCEEEECCCEECCCCCCCHHCCCCEEEEEECHHHHHHHHHHHHHCCC
TRELAIDVTFPEIEKFDNLPEPSVDGPSAFVSIMEGCSKYCTFCVVPYTRGEEVSRPVQP
CCEEEEEEECHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCCCHHCCCHHH
VLKEIQHLADMGVREVNLLGQNVNAYQGVGADGDTLDLADLIRLIRDIDGIDRIRYTTSH
HHHHHHHHHHCCCHHHHHHCCCCCHHCCCCCCCCCCCHHHHHHHHHHCCCHHHEEECCCC
PVEFSEALIQVYEDVPELVSHLHLPVQSGSDRILAMMKRNHMVLEYKSKLRKLKRIRPDI
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEHHHHHHHHHHHHHCCCC
SFSSDFIIGFPGETDRDFEDTMNLIHDIGFDMSFSFIYSARPGTPAADLPDDVDMEVKKQ
CCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHH
RLAILQQRINQNVQDISRKMVGSTQRILVDGFSKKDPGQLKGRTENNRVVNFQCDDTDLI
HHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHHH
GKFVDVTIAKAYSNSLLGTDPRNPS
HHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA