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The map label for this gene is estA [H]

Identifier: 110834756

GI number: 110834756

Start: 2173069

End: 2173737

Strand: Direct

Name: estA [H]

Synonym: ABO_1895

Alternate gene names: 110834756

Gene position: 2173069-2173737 (Clockwise)

Preceding gene: 110834755

Following gene: 110834757

Centisome position: 69.65

GC content: 55.75

Gene sequence:

>669_bases
ATGAGCGAGCTATTACGTTTTGTTGAAATCGAACCCGCCACAGAAGCCACCGCCAGCGTTATCTGGCTGCACGGCCTAGG
TGCCAGTGGCCACGATTTCGAACCTATTGTGCCTGAGCTGCAGCTGCCGGCAGATTTAGCCGTACGGTTTGTGTTTCCTC
ACGCACCGCAAATCCCCGTTACCGTCAATGGCGGCATGGTCATGCCTGCCTGGTACGATATTCTCGCCATGGATATCGAT
CGCAAAGTTGACGAGGCAGGAGTACTGGCATCTGCCAGAGCGGTGGAGATGCTAATCGAGCGAGAAATCGCCCGAGGCAT
CCCCAGCAAACGGATCATCATCGCCGGTTTTTCTCAGGGTGGCGCCGTTGCCTATCAAGCCGCTTTGCGCTACCCCAAAC
CCCTTGCCGGCCTGCTCACCCTGTCCACCTATATGGCCATGCCGGTAATACCCAGCGATGCCAACGCCACACTGCCGGTT
ATGATTTGCCACGGCAGCATGGATCCGGTGGTTCCTGAGCAATTAGGCCAACGAGCCGCAGCCACCCTGAGTGAGCTGGG
TTACTCACCACAATACAAAAGCTATCCTATGGAGCACATGGTCTGCCTAGAGCAAATTCGGGATATTGGCCAATGGCTGA
ATGAACGGCTAATGCCGCCTAACCAATGA

Upstream 100 bases:

>100_bases
ACGCACTGGAAGACCAAACCCAGGCGCTGGAAAAATGCCTCGACGAAGCCCGTTGTCAGCTTGAAGCCGTACGCATCATT
GTCGCGGGAGGAGATTAACC

Downstream 100 bases:

>100_bases
CGAATTCATAAGTAAAACACAGCTTCAAATCTGTCGCAGACAGACCAGTAAAGAGGCGAGTACTTGGCGTCTGCGACGCC
TTCCAGCTTCTGAGCTTTAA

Product: phospholipase/carboxylesterase family protein

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MSELLRFVEIEPATEATASVIWLHGLGASGHDFEPIVPELQLPADLAVRFVFPHAPQIPVTVNGGMVMPAWYDILAMDID
RKVDEAGVLASARAVEMLIEREIARGIPSKRIIIAGFSQGGAVAYQAALRYPKPLAGLLTLSTYMAMPVIPSDANATLPV
MICHGSMDPVVPEQLGQRAAATLSELGYSPQYKSYPMEHMVCLEQIRDIGQWLNERLMPPNQ

Sequences:

>Translated_222_residues
MSELLRFVEIEPATEATASVIWLHGLGASGHDFEPIVPELQLPADLAVRFVFPHAPQIPVTVNGGMVMPAWYDILAMDID
RKVDEAGVLASARAVEMLIEREIARGIPSKRIIIAGFSQGGAVAYQAALRYPKPLAGLLTLSTYMAMPVIPSDANATLPV
MICHGSMDPVVPEQLGQRAAATLSELGYSPQYKSYPMEHMVCLEQIRDIGQWLNERLMPPNQ
>Mature_221_residues
SELLRFVEIEPATEATASVIWLHGLGASGHDFEPIVPELQLPADLAVRFVFPHAPQIPVTVNGGMVMPAWYDILAMDIDR
KVDEAGVLASARAVEMLIEREIARGIPSKRIIIAGFSQGGAVAYQAALRYPKPLAGLLTLSTYMAMPVIPSDANATLPVM
ICHGSMDPVVPEQLGQRAAATLSELGYSPQYKSYPMEHMVCLEQIRDIGQWLNERLMPPNQ

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI9966764, Length=219, Percent_Identity=39.2694063926941, Blast_Score=162, Evalue=2e-40,
Organism=Homo sapiens, GI5453722, Length=217, Percent_Identity=36.8663594470046, Blast_Score=145, Evalue=2e-35,
Organism=Homo sapiens, GI20270341, Length=195, Percent_Identity=28.7179487179487, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17508057, Length=212, Percent_Identity=34.9056603773585, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6323147, Length=228, Percent_Identity=35.0877192982456, Blast_Score=129, Evalue=5e-31,
Organism=Drosophila melanogaster, GI45553055, Length=218, Percent_Identity=34.4036697247706, Blast_Score=140, Evalue=8e-34,
Organism=Drosophila melanogaster, GI45553057, Length=218, Percent_Identity=34.4036697247706, Blast_Score=140, Evalue=8e-34,
Organism=Drosophila melanogaster, GI21357257, Length=218, Percent_Identity=34.4036697247706, Blast_Score=140, Evalue=8e-34,
Organism=Drosophila melanogaster, GI161078179, Length=224, Percent_Identity=27.6785714285714, Blast_Score=93, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 24247; Mature: 24115

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELLRFVEIEPATEATASVIWLHGLGASGHDFEPIVPELQLPADLAVRFVFPHAPQIPV
CCHHHHHHHCCCCHHHHHEEEEEEECCCCCCCCCCCCCCCCCCHHHEEEEECCCCCCCEE
TVNGGMVMPAWYDILAMDIDRKVDEAGVLASARAVEMLIEREIARGIPSKRIIIAGFSQG
EECCCEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
GAVAYQAALRYPKPLAGLLTLSTYMAMPVIPSDANATLPVMICHGSMDPVVPEQLGQRAA
CHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHH
ATLSELGYSPQYKSYPMEHMVCLEQIRDIGQWLNERLMPPNQ
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SELLRFVEIEPATEATASVIWLHGLGASGHDFEPIVPELQLPADLAVRFVFPHAPQIPV
CHHHHHHHCCCCHHHHHEEEEEEECCCCCCCCCCCCCCCCCCHHHEEEEECCCCCCCEE
TVNGGMVMPAWYDILAMDIDRKVDEAGVLASARAVEMLIEREIARGIPSKRIIIAGFSQG
EECCCEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
GAVAYQAALRYPKPLAGLLTLSTYMAMPVIPSDANATLPVMICHGSMDPVVPEQLGQRAA
CHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHH
ATLSELGYSPQYKSYPMEHMVCLEQIRDIGQWLNERLMPPNQ
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA