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The map label for this gene is rnfE [H]

Identifier: 110834475

GI number: 110834475

Start: 1847888

End: 1848604

Strand: Direct

Name: rnfE [H]

Synonym: ABO_1614

Alternate gene names: 110834475

Gene position: 1847888-1848604 (Clockwise)

Preceding gene: 110834474

Following gene: 110834476

Centisome position: 59.22

GC content: 56.76

Gene sequence:

>717_bases
GTGATGAGTGATGTGAACTACAAAAAGATCAGCATGGACGGCTTGTGGAATAACAACCCGGCCACCGTGCAGATTCTCGG
CCTGTGCCCTTTGCTGGCAGTTACCGGGTCCGTGGTCAACGCCATGGGCCTGGGCCTGGCAACGCTGGTGGTGCTGATGG
GATCCAACGCCTGCGTATCACTGATTCGTCATTACGTTACCGATGCTGTGCGCCTGCCTGCCTTCGTAATGATCATCGCG
GCCATGGTAACCGTGATCGAAATGCTGATGCAGGCTTACACCTTTGAGCTGTATGAAATCCTCGGCATTTTCATTCCGCT
GATCGTAACCAACTGCACCGTGCTGGGCCGCGCCGATGCCTTCGCCAGTAAAAATGCGGTTCTGCCCGCTATGGTGGACG
GCGTGATGATGGGCATGGGCTTTTTGGTGATCTTGCTGATTCTCGGTGGCATGCGCGAACTGGTAGGCCAAGGCACACTG
TTCAGCAACATGCAGCTGCTCTTCGGCCCCATGGCTGCCCATTGGGAGCTCACGGTCATCGATAACTACGGCGGTTTCCT
GCTCGCCATCTTGCCGCCCGGCGCCTTCCTTGGGTTGGGGCTGATCATCGCGGGTAAAAACGTCATCGACAAGCGCATCA
AAGAAGTCCAGAAACGCAAAGCCAGCGACGAACCTAAAACCAGCCGTCGGGTGCGCACCACCGGAAAAGTCGGCTAG

Upstream 100 bases:

>100_bases
CGTGCGGTAGTCGGCGCCGTGCACCGCGCGCTACAGTATTTCTCGGCTAACCAGAACCACGTCTTCAGCGCCCCTGCAGA
CACCACCCCGGTGGAGACCT

Downstream 100 bases:

>100_bases
CCAAGCCTGTCGCCTTCTTGCCCTCCTACGCCCTCTTCGTAGGAGCGGCTCAACCAACAAAAGTGGCCACCAAAACCTCA
ATGGTGCCGCGCCCATTTGC

Product: electron transport complex RsxE subunit

Products: NA

Alternate protein names: Nitrogen fixation protein rnfE [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MMSDVNYKKISMDGLWNNNPATVQILGLCPLLAVTGSVVNAMGLGLATLVVLMGSNACVSLIRHYVTDAVRLPAFVMIIA
AMVTVIEMLMQAYTFELYEILGIFIPLIVTNCTVLGRADAFASKNAVLPAMVDGVMMGMGFLVILLILGGMRELVGQGTL
FSNMQLLFGPMAAHWELTVIDNYGGFLLAILPPGAFLGLGLIIAGKNVIDKRIKEVQKRKASDEPKTSRRVRTTGKVG

Sequences:

>Translated_238_residues
MMSDVNYKKISMDGLWNNNPATVQILGLCPLLAVTGSVVNAMGLGLATLVVLMGSNACVSLIRHYVTDAVRLPAFVMIIA
AMVTVIEMLMQAYTFELYEILGIFIPLIVTNCTVLGRADAFASKNAVLPAMVDGVMMGMGFLVILLILGGMRELVGQGTL
FSNMQLLFGPMAAHWELTVIDNYGGFLLAILPPGAFLGLGLIIAGKNVIDKRIKEVQKRKASDEPKTSRRVRTTGKVG
>Mature_238_residues
MMSDVNYKKISMDGLWNNNPATVQILGLCPLLAVTGSVVNAMGLGLATLVVLMGSNACVSLIRHYVTDAVRLPAFVMIIA
AMVTVIEMLMQAYTFELYEILGIFIPLIVTNCTVLGRADAFASKNAVLPAMVDGVMMGMGFLVILLILGGMRELVGQGTL
FSNMQLLFGPMAAHWELTVIDNYGGFLLAILPPGAFLGLGLIIAGKNVIDKRIKEVQKRKASDEPKTSRRVRTTGKVG

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase [H]

COG id: COG4660

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfE

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family [H]

Homologues:

Organism=Escherichia coli, GI1787919, Length=214, Percent_Identity=50.4672897196262, Blast_Score=199, Evalue=1e-52,
Organism=Escherichia coli, GI1787914, Length=142, Percent_Identity=35.2112676056338, Blast_Score=78, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003667
- InterPro:   IPR010968 [H]

Pfam domain/function: PF02508 Rnf-Nqr [H]

EC number: NA

Molecular weight: Translated: 25592; Mature: 25592

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
6.7 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
6.7 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSDVNYKKISMDGLWNNNPATVQILGLCPLLAVTGSVVNAMGLGLATLVVLMGSNACVS
CCCCCCCCEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
LIRHYVTDAVRLPAFVMIIAAMVTVIEMLMQAYTFELYEILGIFIPLIVTNCTVLGRADA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCHH
FASKNAVLPAMVDGVMMGMGFLVILLILGGMRELVGQGTLFSNMQLLFGPMAAHWELTVI
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHHCCCCCCEEEEEE
DNYGGFLLAILPPGAFLGLGLIIAGKNVIDKRIKEVQKRKASDEPKTSRRVRTTGKVG
ECCCCEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MMSDVNYKKISMDGLWNNNPATVQILGLCPLLAVTGSVVNAMGLGLATLVVLMGSNACVS
CCCCCCCCEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
LIRHYVTDAVRLPAFVMIIAAMVTVIEMLMQAYTFELYEILGIFIPLIVTNCTVLGRADA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCHH
FASKNAVLPAMVDGVMMGMGFLVILLILGGMRELVGQGTLFSNMQLLFGPMAAHWELTVI
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHHCCCCCCEEEEEE
DNYGGFLLAILPPGAFLGLGLIIAGKNVIDKRIKEVQKRKASDEPKTSRRVRTTGKVG
ECCCCEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA