Click here to switch to the map view.

The map label for this gene is mesJ [H]

Identifier: 110834021

GI number: 110834021

Start: 1319937

End: 1321238

Strand: Direct

Name: mesJ [H]

Synonym: ABO_1160

Alternate gene names: 110834021

Gene position: 1319937-1321238 (Clockwise)

Preceding gene: 110834020

Following gene: 110834022

Centisome position: 42.3

GC content: 60.37

Gene sequence:

>1302_bases
ATGTCTGATGCTGCTAGGTTCAGCCAGCGCTTGGTTGAACAGGCCCCAGACGGGCCACTGTTGCTTGCCTTAAGCGGTGG
CCTCGATTCCTCTCTATTGCTCCACCTGCTTGTCCAAGCAGGTTTAGCCCCGCGCCTTTGCGCTGTCCATATCGATCATC
AACTGCAGGCGGATAGTCGCGCTTGGTCGCGTTTCTGTGGGGCCTTGGCGGATAAGTATGATATTGCGCTGACGATTGAA
CCGGTCACCGTGAGCATGGCTGAAGGGCTGGAAGCGGGTGCTCGGGACGCCCGCTACCAAGTGCTATTGCCACTGGCTCG
AGCCAAAGGCGCGACGCTGGTGCTGGCACACCATCGCAATGATCAAGCGGAAACGGTGTTGTTCCGGTTGCTACGGGGAG
CGGGGGTACAGGGGCTGTCAGCCATGGGTCAGCACAGCCAGCAACAGGGTGTAACGCTCTGGCGACCGTTACTTGGCCTG
GGTCAGCATGTTCTTGAAAAATGGGGGGCAGAATTATCGCTTAATTGGTTGGAAGACCCCAGTAACCAGGACCAAAGCCT
GCGACGTAATTATCTTCGCCATACTATCCTTCCGGCACTGCGCGAGCGTTGGCCCGGTGCTGACGCCACGCTTGCCCGGG
CCGCAGAGCACATGGCTGAGGCCAATCAATTGCTGACAGAAGTCGCGGTCCAGGATGCCGCAAGCATAGGCGTATCCGGG
GCTGTTCTGCCGCTGCCGCGCCTGGCGGAGTTATCCTTGGCGCGCCAACGTAATGTACTGCGGTGGTGGCTGCAGAAAAA
CCGGGCCTCCGCCCCCAGCGCTGCCGTTCTTGAACAGATTCGAGTGTTGCATCTGGCAGCAGAGGATAGCCAAGCCCGAG
TCGAGTGGGGCGAATATGCGGTTCGCGTGCATCAGGGAGCTCTACACTTTGCTCGTCGTGAAGCCTTCCAGCCCTGGCAA
GGTGACATACTCTGGCCTGAAGGCGAGGCACCGCCGGACTTGCCCCGTTGGCGTTGGACACAGCACGCTGTGGAAGGCGC
AGTATGGGTGCTGCGACCTACTGGGGATATTCAACTCCGCTCATTCACCGGTGCCCTGACTCTGCGGCGCAATGGCTTGC
GCCAGAAAATCAAAGAGCTCTGGCGTGCGGCAGGCGTGCCGCCTTGGCAACGCCGGCAGTGGCCGCTACTTTGCCGAGAT
GGCGAGGTGGTGAGTGTGCCGCTGGTGGGGCTGGCCGATGGCGAAGCGCTCGAGCGCGCTGAGCGCTGGTATTTATTACC
GGCTGGTTCTGCGTCTGACTAA

Upstream 100 bases:

>100_bases
AATCGCTTTGTTCCATGGAGACTGACGCCCTCATTAACCGCCGTTACGAACGTTTGATGAGTTACGGCAACGTGGTTAGC
GACCCCGCCGACGAATAGTC

Downstream 100 bases:

>100_bases
TAATCCTGTCGGGTTGCCTGTGTGGCACCGGCTCATTGTCCGTTCTGTCTCACAGGGAAATACGACAGGTAGCCTCGTTT
CCTGCTGGCGGTTCAGACAA

Product: cell cycle protein MesJ

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSDAARFSQRLVEQAPDGPLLLALSGGLDSSLLLHLLVQAGLAPRLCAVHIDHQLQADSRAWSRFCGALADKYDIALTIE
PVTVSMAEGLEAGARDARYQVLLPLARAKGATLVLAHHRNDQAETVLFRLLRGAGVQGLSAMGQHSQQQGVTLWRPLLGL
GQHVLEKWGAELSLNWLEDPSNQDQSLRRNYLRHTILPALRERWPGADATLARAAEHMAEANQLLTEVAVQDAASIGVSG
AVLPLPRLAELSLARQRNVLRWWLQKNRASAPSAAVLEQIRVLHLAAEDSQARVEWGEYAVRVHQGALHFARREAFQPWQ
GDILWPEGEAPPDLPRWRWTQHAVEGAVWVLRPTGDIQLRSFTGALTLRRNGLRQKIKELWRAAGVPPWQRRQWPLLCRD
GEVVSVPLVGLADGEALERAERWYLLPAGSASD

Sequences:

>Translated_433_residues
MSDAARFSQRLVEQAPDGPLLLALSGGLDSSLLLHLLVQAGLAPRLCAVHIDHQLQADSRAWSRFCGALADKYDIALTIE
PVTVSMAEGLEAGARDARYQVLLPLARAKGATLVLAHHRNDQAETVLFRLLRGAGVQGLSAMGQHSQQQGVTLWRPLLGL
GQHVLEKWGAELSLNWLEDPSNQDQSLRRNYLRHTILPALRERWPGADATLARAAEHMAEANQLLTEVAVQDAASIGVSG
AVLPLPRLAELSLARQRNVLRWWLQKNRASAPSAAVLEQIRVLHLAAEDSQARVEWGEYAVRVHQGALHFARREAFQPWQ
GDILWPEGEAPPDLPRWRWTQHAVEGAVWVLRPTGDIQLRSFTGALTLRRNGLRQKIKELWRAAGVPPWQRRQWPLLCRD
GEVVSVPLVGLADGEALERAERWYLLPAGSASD
>Mature_432_residues
SDAARFSQRLVEQAPDGPLLLALSGGLDSSLLLHLLVQAGLAPRLCAVHIDHQLQADSRAWSRFCGALADKYDIALTIEP
VTVSMAEGLEAGARDARYQVLLPLARAKGATLVLAHHRNDQAETVLFRLLRGAGVQGLSAMGQHSQQQGVTLWRPLLGLG
QHVLEKWGAELSLNWLEDPSNQDQSLRRNYLRHTILPALRERWPGADATLARAAEHMAEANQLLTEVAVQDAASIGVSGA
VLPLPRLAELSLARQRNVLRWWLQKNRASAPSAAVLEQIRVLHLAAEDSQARVEWGEYAVRVHQGALHFARREAFQPWQG
DILWPEGEAPPDLPRWRWTQHAVEGAVWVLRPTGDIQLRSFTGALTLRRNGLRQKIKELWRAAGVPPWQRRQWPLLCRDG
EVVSVPLVGLADGEALERAERWYLLPAGSASD

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=428, Percent_Identity=35.981308411215, Blast_Score=178, Evalue=8e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 47975; Mature: 47844

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAARFSQRLVEQAPDGPLLLALSGGLDSSLLLHLLVQAGLAPRLCAVHIDHQLQADSR
CCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCHHCCHHH
AWSRFCGALADKYDIALTIEPVTVSMAEGLEAGARDARYQVLLPLARAKGATLVLAHHRN
HHHHHHHHHCCCEEEEEEEECEEEEHHHHHCCCCCCCCEEEEEEHHCCCCCEEEEEECCC
DQAETVLFRLLRGAGVQGLSAMGQHSQQQGVTLWRPLLGLGQHVLEKWGAELSLNWLEDP
CHHHHHHHHHHHCCCCCHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
SNQDQSLRRNYLRHTILPALRERWPGADATLARAAEHMAEANQLLTEVAVQDAASIGVSG
CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AVLPLPRLAELSLARQRNVLRWWLQKNRASAPSAAVLEQIRVLHLAAEDSQARVEWGEYA
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEECCCCCCHHHHHHHH
VRVHQGALHFARREAFQPWQGDILWPEGEAPPDLPRWRWTQHAVEGAVWVLRPTGDIQLR
HHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCHHHHHHHCCEEEEEECCCCEEEE
SFTGALTLRRNGLRQKIKELWRAAGVPPWQRRQWPLLCRDGEVVSVPLVGLADGEALERA
EECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCEEEEEEEECCCCHHHHHH
ERWYLLPAGSASD
HCEEEECCCCCCC
>Mature Secondary Structure 
SDAARFSQRLVEQAPDGPLLLALSGGLDSSLLLHLLVQAGLAPRLCAVHIDHQLQADSR
CHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCHHCCHHH
AWSRFCGALADKYDIALTIEPVTVSMAEGLEAGARDARYQVLLPLARAKGATLVLAHHRN
HHHHHHHHHCCCEEEEEEEECEEEEHHHHHCCCCCCCCEEEEEEHHCCCCCEEEEEECCC
DQAETVLFRLLRGAGVQGLSAMGQHSQQQGVTLWRPLLGLGQHVLEKWGAELSLNWLEDP
CHHHHHHHHHHHCCCCCHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
SNQDQSLRRNYLRHTILPALRERWPGADATLARAAEHMAEANQLLTEVAVQDAASIGVSG
CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AVLPLPRLAELSLARQRNVLRWWLQKNRASAPSAAVLEQIRVLHLAAEDSQARVEWGEYA
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEECCCCCCHHHHHHHH
VRVHQGALHFARREAFQPWQGDILWPEGEAPPDLPRWRWTQHAVEGAVWVLRPTGDIQLR
HHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCHHHHHHHCCEEEEEECCCCEEEE
SFTGALTLRRNGLRQKIKELWRAAGVPPWQRRQWPLLCRDGEVVSVPLVGLADGEALERA
EECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCEEEEEEEECCCCHHHHHH
ERWYLLPAGSASD
HCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA