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The map label for this gene is amtB1 [H]

Identifier: 110833985

GI number: 110833985

Start: 1276250

End: 1277536

Strand: Direct

Name: amtB1 [H]

Synonym: ABO_1124

Alternate gene names: 110833985

Gene position: 1276250-1277536 (Clockwise)

Preceding gene: 110833984

Following gene: 110833986

Centisome position: 40.9

GC content: 55.24

Gene sequence:

>1287_bases
ATGAAAAAATTGATTGCTGTATTGTTGATGCTGCCCTCGTTGGCTCTGGCCGATGGCCTGGATACCGGTGACAGCGCCTG
GATTCTCACTGCCACCGCGTTGGTGTTATTTATGACGCTGCCGGGTTTGAGCCTGTTCTACGGTGGGCTGGTACGCAGCA
AGAATATCCTCTCGGTGTTGATGCAGTGTTTTGCTATTACCTGCGCTGTGTCGCTAGTGTGGTTGGTCTGTGGTTATTCT
CTCGCTTTTTCCGATGGGGGCAGCATGAATGCCTGGATTGGTGGCTTGGACCATGTTTTCTTTTCTGGAATCAACCGCGA
TACCTTGGAAGGCACCTTACCGAAAAGTTTGCATGGGTTGTTTCAGATGACCTTTGCGATCATCACCCCGGCGCTGATCG
TCGGTGCCTTTGCCGAGCGTATGCGCTTCGGTCCCATGCTGGCGTTTTCTATTCTCTGGTTGTTGGGTGTGTATATTCCT
GTTTGTCATTGGGTTTGGGGCGGCGGCTGGTTAGCCGATCTGGGGCTGTATGATTTTGCCGGAGGCACCGTGGTGCATAT
CACCGCCGGTGTGGCGGCGTTGGTGGCAGCGGTGGTGCTCGGTAACCGTAAAGGCTTCCCCACCCAAGGCATGATGCCGC
ACAACATGACCATGACGGTAACCGGTGCCGGCATGTTGTGGGTGGGCTGGTTCGGCTTTAACGGTGGTTCTGCGTTAGCC
GCCAATGGTGATGCAGCCATGGCGATGCTGGTAACGCACATTTCTGCCGCCGCCGGTTCACTGGCCTGGATGACCATGGA
ATGGAAAAAATTCGGTAAGCCGTCTGCTCTGGGCATTGCCACGGGCATGGTTGCAGGGTTGGGAACCATTACCCCGGCGT
CGGGCTTTGTTAGTCCGGCCGCTGCGCTGGTGATTGGTCTATCGGCTGGCGTGATCTGTTTTTACATGGTGATCTTTGTT
AAGCACAAGCTGAAAATCGATGACTCCCTTGATGTTTTCCCTGTCCACGGTATTGGCGGCATCCTTGGCACTTTGTTGGC
AGGGGTGTTTGCCTCTACGGAGTTGGGTCTGTTCTCCGGCTACGGGTTTGCCAAACCGGAATACACCATGCTGGATCAGC
TGGGTGTGCAGGCGTTGGGTGTGGTGGCAACCTTTGTGTACACCGCCTTGGTGACGTGGGGCTTGCTCAAGCTGGTAGGC
TTGTTTGCACCGTTACGGGTCACCGCCGAGGAAGAGACCCAGGGCCTGGATATTGCGTTGCATGAAGAGCGCGGTTATGA
CCTTTGA

Upstream 100 bases:

>100_bases
GGCAAGATTGGTGACGGTAAAATTTTTGTGACGGATCTTGAGCAGGTTATCCGTATCCGTACCGGAGAAATTGGCAGCGA
AGCCATTTAAGGGGCCCATA

Downstream 100 bases:

>100_bases
TTACAGCACTGAAATCCGATTATCAAGCAACCGATAAGCTCGGAATTTTGCCAGGCTTTTTTGGCCTGTAAGGACTCATC
TGCGGCTATTGCCACCCTCT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKKLIAVLLMLPSLALADGLDTGDSAWILTATALVLFMTLPGLSLFYGGLVRSKNILSVLMQCFAITCAVSLVWLVCGYS
LAFSDGGSMNAWIGGLDHVFFSGINRDTLEGTLPKSLHGLFQMTFAIITPALIVGAFAERMRFGPMLAFSILWLLGVYIP
VCHWVWGGGWLADLGLYDFAGGTVVHITAGVAALVAAVVLGNRKGFPTQGMMPHNMTMTVTGAGMLWVGWFGFNGGSALA
ANGDAAMAMLVTHISAAAGSLAWMTMEWKKFGKPSALGIATGMVAGLGTITPASGFVSPAAALVIGLSAGVICFYMVIFV
KHKLKIDDSLDVFPVHGIGGILGTLLAGVFASTELGLFSGYGFAKPEYTMLDQLGVQALGVVATFVYTALVTWGLLKLVG
LFAPLRVTAEEETQGLDIALHEERGYDL

Sequences:

>Translated_428_residues
MKKLIAVLLMLPSLALADGLDTGDSAWILTATALVLFMTLPGLSLFYGGLVRSKNILSVLMQCFAITCAVSLVWLVCGYS
LAFSDGGSMNAWIGGLDHVFFSGINRDTLEGTLPKSLHGLFQMTFAIITPALIVGAFAERMRFGPMLAFSILWLLGVYIP
VCHWVWGGGWLADLGLYDFAGGTVVHITAGVAALVAAVVLGNRKGFPTQGMMPHNMTMTVTGAGMLWVGWFGFNGGSALA
ANGDAAMAMLVTHISAAAGSLAWMTMEWKKFGKPSALGIATGMVAGLGTITPASGFVSPAAALVIGLSAGVICFYMVIFV
KHKLKIDDSLDVFPVHGIGGILGTLLAGVFASTELGLFSGYGFAKPEYTMLDQLGVQALGVVATFVYTALVTWGLLKLVG
LFAPLRVTAEEETQGLDIALHEERGYDL
>Mature_428_residues
MKKLIAVLLMLPSLALADGLDTGDSAWILTATALVLFMTLPGLSLFYGGLVRSKNILSVLMQCFAITCAVSLVWLVCGYS
LAFSDGGSMNAWIGGLDHVFFSGINRDTLEGTLPKSLHGLFQMTFAIITPALIVGAFAERMRFGPMLAFSILWLLGVYIP
VCHWVWGGGWLADLGLYDFAGGTVVHITAGVAALVAAVVLGNRKGFPTQGMMPHNMTMTVTGAGMLWVGWFGFNGGSALA
ANGDAAMAMLVTHISAAAGSLAWMTMEWKKFGKPSALGIATGMVAGLGTITPASGFVSPAAALVIGLSAGVICFYMVIFV
KHKLKIDDSLDVFPVHGIGGILGTLLAGVFASTELGLFSGYGFAKPEYTMLDQLGVQALGVVATFVYTALVTWGLLKLVG
LFAPLRVTAEEETQGLDIALHEERGYDL

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=429, Percent_Identity=45.2214452214452, Blast_Score=340, Evalue=9e-95,
Organism=Caenorhabditis elegans, GI17550180, Length=445, Percent_Identity=28.314606741573, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17550182, Length=443, Percent_Identity=28.6681715575621, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI32564042, Length=428, Percent_Identity=25.9345794392523, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI133931100, Length=445, Percent_Identity=24.7191011235955, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324187, Length=430, Percent_Identity=37.4418604651163, Blast_Score=237, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6325396, Length=416, Percent_Identity=33.6538461538462, Blast_Score=217, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6321559, Length=412, Percent_Identity=32.0388349514563, Blast_Score=200, Evalue=4e-52,
Organism=Drosophila melanogaster, GI161078314, Length=424, Percent_Identity=28.5377358490566, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45035; Mature: 45035

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLIAVLLMLPSLALADGLDTGDSAWILTATALVLFMTLPGLSLFYGGLVRSKNILSVL
CHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHH
MQCFAITCAVSLVWLVCGYSLAFSDGGSMNAWIGGLDHVFFSGINRDTLEGTLPKSLHGL
HHHHHHHHHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
FQMTFAIITPALIVGAFAERMRFGPMLAFSILWLLGVYIPVCHWVWGGGWLADLGLYDFA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHCC
GGTVVHITAGVAALVAAVVLGNRKGFPTQGMMPHNMTMTVTGAGMLWVGWFGFNGGSALA
CCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCEEEEEEEEECCCCEEE
ANGDAAMAMLVTHISAAAGSLAWMTMEWKKFGKPSALGIATGMVAGLGTITPASGFVSPA
CCCHHHHHHHHHHHHHHCCCHHEEEEEHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHH
AALVIGLSAGVICFYMVIFVKHKLKIDDSLDVFPVHGIGGILGTLLAGVFASTELGLFSG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCHHCC
YGFAKPEYTMLDQLGVQALGVVATFVYTALVTWGLLKLVGLFAPLRVTAEEETQGLDIAL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCEEEE
HEERGYDL
ECCCCCCC
>Mature Secondary Structure
MKKLIAVLLMLPSLALADGLDTGDSAWILTATALVLFMTLPGLSLFYGGLVRSKNILSVL
CHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHH
MQCFAITCAVSLVWLVCGYSLAFSDGGSMNAWIGGLDHVFFSGINRDTLEGTLPKSLHGL
HHHHHHHHHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
FQMTFAIITPALIVGAFAERMRFGPMLAFSILWLLGVYIPVCHWVWGGGWLADLGLYDFA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHCC
GGTVVHITAGVAALVAAVVLGNRKGFPTQGMMPHNMTMTVTGAGMLWVGWFGFNGGSALA
CCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCEEEEEEEEECCCCEEE
ANGDAAMAMLVTHISAAAGSLAWMTMEWKKFGKPSALGIATGMVAGLGTITPASGFVSPA
CCCHHHHHHHHHHHHHHCCCHHEEEEEHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHH
AALVIGLSAGVICFYMVIFVKHKLKIDDSLDVFPVHGIGGILGTLLAGVFASTELGLFSG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCHHCC
YGFAKPEYTMLDQLGVQALGVVATFVYTALVTWGLLKLVGLFAPLRVTAEEETQGLDIAL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCEEEE
HEERGYDL
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]