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The map label for this gene is nirB [H]

Identifier: 110833947

GI number: 110833947

Start: 1229424

End: 1231955

Strand: Direct

Name: nirB [H]

Synonym: ABO_1086

Alternate gene names: 110833947

Gene position: 1229424-1231955 (Clockwise)

Preceding gene: 110833944

Following gene: 110833948

Centisome position: 39.4

GC content: 55.92

Gene sequence:

>2532_bases
ATGGCAAAGCAACAACTTCTTCTGATCGGCCACGGGATGGTCGGACAACGTTATCTAGAGTCTCTGGTAGCCCTGGGTGG
CCGAGAGCACTTTGATATTACGGTACTGTGTGCCGAGCCGCTGCCGGCCTATGACCGAGTAGGGCTGACCAGTTATTTTT
CCGGCAAGACCGCCGACGACCTGTCGCTAGTGCCGGAAGGGTTTTTCGAAGAGCACGGCATCACGCTGAAACTTAACACC
AAGGTCAACGCGATTGATCGCGCGGGCAAAACGGTCACCACCAATACGGGTGAAACCCTGCCTTACGACAAGGTCGTGCT
GGCCACCGGCTCCTATCCTTTCATCCCGCCGATTCCCGGCAACGACCGCGACAACTGCTTTGTGTACCGCACCATTGATG
ACCTGGATGCCATGATTGCTGCCAGCAAGAACGGCAAGGTGGGCGCCGTGGTCGGTGGAGGGTTGCTGGGTTTAGAAGCG
GCTAAGGCGCTTCATGACTTGGGGCTTGAAACGCATGTGGTGGAATTCGCTCCCCGGCTGATGGCGGTGCAAGTGGATGA
TTTTGGCGGCGGCTTGCTGAAAGACAAGATTCAGGAGCTGGGTGTCACCGTGCACACTAGCACCGGTACACAAGATATTG
TCGACGGTGAAACCTGCACTCATCGAATGAACTTCGGTGACGACAAGTATTTGGAAACCGATGTCATTGTTTTCTCCGCC
GGGATCCGTCCTCAGGACGAATTGGGCAGGGCTTGTGAGCTGGCCATCGGCGAGCGCGGCGGCATTGTGGTCAATAACCA
ATGTGTCACTTCCGACGAGCATATTTATGCGATCGGTGAAGTGGCGCTATGGGAAGGGCGCATTTTCGGGCTGGTGGCGC
CGGGTTACCAGATGGCAGAAGTGGCCGCAGAACATCTGATAGGAAAAGCAGAAAAGCAGTTTGCCGGTGCGGACATGAGC
ACCAAGCTGAAGCTGATGGGCGTGGATGTGGCATCCATCGGAGATGCTCACGGCAGCACGCCGGGCTGCCGCAGTACCGT
GTTTATGGATCAAACCGCGGGTATCTACAAAAAAGCGGTGATCAGCGCGTGTGGCAAGAAACTGTTGGGTGCGGTGTTGG
TGGGTGATGCTGCCGATTACGGTAACTTGTTGCAACTTTGCCTGAATGAAATGGACTTGCCCGGTGATCCGGCGGCCCTG
GTGGCACCGGTGTCGGACGGCGCGCCTCTGCTGGGCGCCGATGCATTGCCCGATTCGGCGCAGATTTGTTCCTGTAATAA
CGTCTCCAAGGGCGACCTGTGTGCGGCCATCGACGATGGCGCCCAGACCGTGGGTGACTTGAAAGCTTGCACCAAGGCAT
CGGCTACCTGTGGGGGTTGCTCCGCGCTGGTGAAACAGGTGCTGGATAGTGAGCTGGAAAAACGCGGTGTGGAAGTGAAC
AACCATCTCTGCGAACACTTTCCGTATTCCCGTCAAGAAATCTATCACCTGGTACGCGTCGGCCAGATCACCACTTTTGA
TGCCTTTCTGGAAGCCCATGGTAGCGGCGATGGTTGTGACATCTGTAAACCGACCGTGGCCAACGTGCTGGCTTCCTGCT
GGAACGACTGGGTGCTCAACAAACCCCATGCTGGCCTGCAGGACACCAATGATTACTTCCTTGGCAACATGCAGCGCGAC
GGCACGTATTCCATTGTGCCCCGGGTGGCCGGCGGTGAAATCACGCCGGACAAACTGATTGTGCTGGGCGAGGTGGCCAA
GCAATACGATCTCTATACCAAGATTACGGGTGGCCAGCGGGTGGACTTGTTCGGTGCCCGTGTGGAGCAGCTGCCGGAAA
TCTGGAAGATTTTGGTCGATGCCGGTTTTGAAACTGGGCACGCCTACGGTAAATCCCTGCGCACGGTGAAGTCCTGTGTG
GGCTCTACCTGGTGTCGTTATGGGGTGAAAGATTCCGTGGGCACCGCTATTCAAGTGGAGGACCGCTACAAAGGCCTGCG
TAGCCCGCACAAGATCAAAATGGCGGTGAGTGGCTGTACCCGCGAATGTGCAGAAGCTCAGAGCAAAGACGTGGGCGTAA
TTGCCACGGAGAAAGGCTGGAACCTGTATGTGTGCGGTAACGGGGGTATGCGCCCGCGCCATGCGGATCTGTTTGCCTCC
GATCTCACCGATGAACAGCTGATTAAAACCATTGATCGTTTCTTGATGTTCTACATCAAAACAGCGGATCGTTTGCAGCG
CACCAGTGTCTGGCTGGAAAACTTAGAAGGCGGGTTGGATTACCTGAAAGACGTGATCCTCAACGATTCCTTGGGCATGG
GCGATGAGCTTGAAGCACAGATGCAACACGTGATCGAGACATACCAGTGTGAGTGGAAGTCCACCATCGAAAATCCCGAA
GCGGTGAAACGTTTCAATCACTTTATCAATGATACCGCTGAAGCGGATGACAACCTGACCTACGTGCGTGAACGTGGCCA
GCGACGTCCCGCCTACGAGCATGAACGTATCGAGCTGGTTGAGGAGGCGTAA

Upstream 100 bases:

>100_bases
ATAAAAAAGGAAATAACCTTATTTATTTCAATGGGTTAGTTAGTTGGCATAAGGCTTGCTGTAGTGGTGGTGTCAGTTAC
GCACCGCGAGGGTGAGCCTT

Downstream 100 bases:

>100_bases
GCCATGAACAATGCAGTAGCGGTTGAATGGAAAACAATCTGCAGCGTCGACGATGTGTTGCCCGGCACCGGAGTGGGAGT
ACGCCTGGCCACGGGGCAGG

Product: nitrite reductase [NAD(P)H]

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 843; Mature: 842

Protein sequence:

>843_residues
MAKQQLLLIGHGMVGQRYLESLVALGGREHFDITVLCAEPLPAYDRVGLTSYFSGKTADDLSLVPEGFFEEHGITLKLNT
KVNAIDRAGKTVTTNTGETLPYDKVVLATGSYPFIPPIPGNDRDNCFVYRTIDDLDAMIAASKNGKVGAVVGGGLLGLEA
AKALHDLGLETHVVEFAPRLMAVQVDDFGGGLLKDKIQELGVTVHTSTGTQDIVDGETCTHRMNFGDDKYLETDVIVFSA
GIRPQDELGRACELAIGERGGIVVNNQCVTSDEHIYAIGEVALWEGRIFGLVAPGYQMAEVAAEHLIGKAEKQFAGADMS
TKLKLMGVDVASIGDAHGSTPGCRSTVFMDQTAGIYKKAVISACGKKLLGAVLVGDAADYGNLLQLCLNEMDLPGDPAAL
VAPVSDGAPLLGADALPDSAQICSCNNVSKGDLCAAIDDGAQTVGDLKACTKASATCGGCSALVKQVLDSELEKRGVEVN
NHLCEHFPYSRQEIYHLVRVGQITTFDAFLEAHGSGDGCDICKPTVANVLASCWNDWVLNKPHAGLQDTNDYFLGNMQRD
GTYSIVPRVAGGEITPDKLIVLGEVAKQYDLYTKITGGQRVDLFGARVEQLPEIWKILVDAGFETGHAYGKSLRTVKSCV
GSTWCRYGVKDSVGTAIQVEDRYKGLRSPHKIKMAVSGCTRECAEAQSKDVGVIATEKGWNLYVCGNGGMRPRHADLFAS
DLTDEQLIKTIDRFLMFYIKTADRLQRTSVWLENLEGGLDYLKDVILNDSLGMGDELEAQMQHVIETYQCEWKSTIENPE
AVKRFNHFINDTAEADDNLTYVRERGQRRPAYEHERIELVEEA

Sequences:

>Translated_843_residues
MAKQQLLLIGHGMVGQRYLESLVALGGREHFDITVLCAEPLPAYDRVGLTSYFSGKTADDLSLVPEGFFEEHGITLKLNT
KVNAIDRAGKTVTTNTGETLPYDKVVLATGSYPFIPPIPGNDRDNCFVYRTIDDLDAMIAASKNGKVGAVVGGGLLGLEA
AKALHDLGLETHVVEFAPRLMAVQVDDFGGGLLKDKIQELGVTVHTSTGTQDIVDGETCTHRMNFGDDKYLETDVIVFSA
GIRPQDELGRACELAIGERGGIVVNNQCVTSDEHIYAIGEVALWEGRIFGLVAPGYQMAEVAAEHLIGKAEKQFAGADMS
TKLKLMGVDVASIGDAHGSTPGCRSTVFMDQTAGIYKKAVISACGKKLLGAVLVGDAADYGNLLQLCLNEMDLPGDPAAL
VAPVSDGAPLLGADALPDSAQICSCNNVSKGDLCAAIDDGAQTVGDLKACTKASATCGGCSALVKQVLDSELEKRGVEVN
NHLCEHFPYSRQEIYHLVRVGQITTFDAFLEAHGSGDGCDICKPTVANVLASCWNDWVLNKPHAGLQDTNDYFLGNMQRD
GTYSIVPRVAGGEITPDKLIVLGEVAKQYDLYTKITGGQRVDLFGARVEQLPEIWKILVDAGFETGHAYGKSLRTVKSCV
GSTWCRYGVKDSVGTAIQVEDRYKGLRSPHKIKMAVSGCTRECAEAQSKDVGVIATEKGWNLYVCGNGGMRPRHADLFAS
DLTDEQLIKTIDRFLMFYIKTADRLQRTSVWLENLEGGLDYLKDVILNDSLGMGDELEAQMQHVIETYQCEWKSTIENPE
AVKRFNHFINDTAEADDNLTYVRERGQRRPAYEHERIELVEEA
>Mature_842_residues
AKQQLLLIGHGMVGQRYLESLVALGGREHFDITVLCAEPLPAYDRVGLTSYFSGKTADDLSLVPEGFFEEHGITLKLNTK
VNAIDRAGKTVTTNTGETLPYDKVVLATGSYPFIPPIPGNDRDNCFVYRTIDDLDAMIAASKNGKVGAVVGGGLLGLEAA
KALHDLGLETHVVEFAPRLMAVQVDDFGGGLLKDKIQELGVTVHTSTGTQDIVDGETCTHRMNFGDDKYLETDVIVFSAG
IRPQDELGRACELAIGERGGIVVNNQCVTSDEHIYAIGEVALWEGRIFGLVAPGYQMAEVAAEHLIGKAEKQFAGADMST
KLKLMGVDVASIGDAHGSTPGCRSTVFMDQTAGIYKKAVISACGKKLLGAVLVGDAADYGNLLQLCLNEMDLPGDPAALV
APVSDGAPLLGADALPDSAQICSCNNVSKGDLCAAIDDGAQTVGDLKACTKASATCGGCSALVKQVLDSELEKRGVEVNN
HLCEHFPYSRQEIYHLVRVGQITTFDAFLEAHGSGDGCDICKPTVANVLASCWNDWVLNKPHAGLQDTNDYFLGNMQRDG
TYSIVPRVAGGEITPDKLIVLGEVAKQYDLYTKITGGQRVDLFGARVEQLPEIWKILVDAGFETGHAYGKSLRTVKSCVG
STWCRYGVKDSVGTAIQVEDRYKGLRSPHKIKMAVSGCTRECAEAQSKDVGVIATEKGWNLYVCGNGGMRPRHADLFASD
LTDEQLIKTIDRFLMFYIKTADRLQRTSVWLENLEGGLDYLKDVILNDSLGMGDELEAQMQHVIETYQCEWKSTIENPEA
VKRFNHFINDTAEADDNLTYVRERGQRRPAYEHERIELVEEA

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=327, Percent_Identity=27.217125382263, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI226437568, Length=327, Percent_Identity=27.217125382263, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI21389617, Length=327, Percent_Identity=27.217125382263, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI1789765, Length=847, Percent_Identity=58.6776859504132, Blast_Score=1010, Evalue=0.0,
Organism=Escherichia coli, GI1789065, Length=305, Percent_Identity=25.5737704918033, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI1788892, Length=244, Percent_Identity=24.1803278688525, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17559934, Length=281, Percent_Identity=27.4021352313167, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24585130, Length=285, Percent_Identity=22.8070175438596, Blast_Score=77, Evalue=7e-14,
Organism=Drosophila melanogaster, GI281359715, Length=324, Percent_Identity=25.9259259259259, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281359713, Length=324, Percent_Identity=25.9259259259259, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24639250, Length=324, Percent_Identity=25.9259259259259, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI18543267, Length=324, Percent_Identity=25.9259259259259, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24639252, Length=324, Percent_Identity=25.9259259259259, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24639257, Length=324, Percent_Identity=25.9259259259259, Blast_Score=72, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR012748
- InterPro:   IPR017881
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327
- InterPro:   IPR017941 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 91356; Mature: 91224

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00022 EGF_1 ; PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQQLLLIGHGMVGQRYLESLVALGGREHFDITVLCAEPLPAYDRVGLTSYFSGKTADD
CCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHCCCHHHCCCCCCCC
LSLVPEGFFEEHGITLKLNTKVNAIDRAGKTVTTNTGETLPYDKVVLATGSYPFIPPIPG
HHHCCCCHHHHCCEEEEECCCCCHHHHCCCEEECCCCCCCCCCCEEEEECCCCCCCCCCC
NDRDNCFVYRTIDDLDAMIAASKNGKVGAVVGGGLLGLEAAKALHDLGLETHVVEFAPRL
CCCCCEEEEEEHHHHHHHHEECCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHCCCE
MAVQVDDFGGGLLKDKIQELGVTVHTSTGTQDIVDGETCTHRMNFGDDKYLETDVIVFSA
EEEEECCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCHHEEECCCCCCCEEEEEEEEEEC
GIRPQDELGRACELAIGERGGIVVNNQCVTSDEHIYAIGEVALWEGRIFGLVAPGYQMAE
CCCCHHHHCCCEEEEECCCCCEEECCEEECCCCCEEEEEEEEEECCEEEEEECCCHHHHH
VAAEHLIGKAEKQFAGADMSTKLKLMGVDVASIGDAHGSTPGCRSTVFMDQTAGIYKKAV
HHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHCCCCCCCCCCCCEEEEECCHHHHHHHHH
ISACGKKLLGAVLVGDAADYGNLLQLCLNEMDLPGDPAALVAPVSDGAPLLGADALPDSA
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
QICSCNNVSKGDLCAAIDDGAQTVGDLKACTKASATCGGCSALVKQVLDSELEKRGVEVN
CEECCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHH
NHLCEHFPYSRQEIYHLVRVGQITTFDAFLEAHGSGDGCDICKPTVANVLASCWNDWVLN
HHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCC
KPHAGLQDTNDYFLGNMQRDGTYSIVPRVAGGEITPDKLIVLGEVAKQYDLYTKITGGQR
CCCCCCCCCCCCEECCCCCCCCEEEEEECCCCCCCCCCEEEEHHHHHHHHHEEEECCCCE
VDLFGARVEQLPEIWKILVDAGFETGHAYGKSLRTVKSCVGSTWCRYGVKDSVGTAIQVE
EEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEH
DRYKGLRSPHKIKMAVSGCTRECAEAQSKDVGVIATEKGWNLYVCGNGGMRPRHADLFAS
HHHHCCCCCCEEEEEHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCCCCHHHHHHH
DLTDEQLIKTIDRFLMFYIKTADRLQRTSVWLENLEGGLDYLKDVILNDSLGMGDELEAQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
MQHVIETYQCEWKSTIENPEAVKRFNHFINDTAEADDNLTYVRERGQRRPAYEHERIELV
HHHHHHHHHCCHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHC
EEA
CCC
>Mature Secondary Structure 
AKQQLLLIGHGMVGQRYLESLVALGGREHFDITVLCAEPLPAYDRVGLTSYFSGKTADD
CCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHCCCHHHCCCCCCCC
LSLVPEGFFEEHGITLKLNTKVNAIDRAGKTVTTNTGETLPYDKVVLATGSYPFIPPIPG
HHHCCCCHHHHCCEEEEECCCCCHHHHCCCEEECCCCCCCCCCCEEEEECCCCCCCCCCC
NDRDNCFVYRTIDDLDAMIAASKNGKVGAVVGGGLLGLEAAKALHDLGLETHVVEFAPRL
CCCCCEEEEEEHHHHHHHHEECCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHCCCE
MAVQVDDFGGGLLKDKIQELGVTVHTSTGTQDIVDGETCTHRMNFGDDKYLETDVIVFSA
EEEEECCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCHHEEECCCCCCCEEEEEEEEEEC
GIRPQDELGRACELAIGERGGIVVNNQCVTSDEHIYAIGEVALWEGRIFGLVAPGYQMAE
CCCCHHHHCCCEEEEECCCCCEEECCEEECCCCCEEEEEEEEEECCEEEEEECCCHHHHH
VAAEHLIGKAEKQFAGADMSTKLKLMGVDVASIGDAHGSTPGCRSTVFMDQTAGIYKKAV
HHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHCCCCCCCCCCCCEEEEECCHHHHHHHHH
ISACGKKLLGAVLVGDAADYGNLLQLCLNEMDLPGDPAALVAPVSDGAPLLGADALPDSA
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
QICSCNNVSKGDLCAAIDDGAQTVGDLKACTKASATCGGCSALVKQVLDSELEKRGVEVN
CEECCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHH
NHLCEHFPYSRQEIYHLVRVGQITTFDAFLEAHGSGDGCDICKPTVANVLASCWNDWVLN
HHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCC
KPHAGLQDTNDYFLGNMQRDGTYSIVPRVAGGEITPDKLIVLGEVAKQYDLYTKITGGQR
CCCCCCCCCCCCEECCCCCCCCEEEEEECCCCCCCCCCEEEEHHHHHHHHHEEEECCCCE
VDLFGARVEQLPEIWKILVDAGFETGHAYGKSLRTVKSCVGSTWCRYGVKDSVGTAIQVE
EEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEH
DRYKGLRSPHKIKMAVSGCTRECAEAQSKDVGVIATEKGWNLYVCGNGGMRPRHADLFAS
HHHHCCCCCCEEEEEHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCCCCHHHHHHH
DLTDEQLIKTIDRFLMFYIKTADRLQRTSVWLENLEGGLDYLKDVILNDSLGMGDELEAQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
MQHVIETYQCEWKSTIENPEAVKRFNHFINDTAEADDNLTYVRERGQRRPAYEHERIELV
HHHHHHHHHCCHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHC
EEA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8468296 [H]