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The map label for this gene is pcaF [H]

Identifier: 110833844

GI number: 110833844

Start: 1107771

End: 1108979

Strand: Direct

Name: pcaF [H]

Synonym: ABO_0983

Alternate gene names: 110833844

Gene position: 1107771-1108979 (Clockwise)

Preceding gene: 110833843

Following gene: 110833845

Centisome position: 35.5

GC content: 57.4

Gene sequence:

>1209_bases
ATGCTTGATGCCTATATCTACGATGGTCTGCGTACCCCTTTTGGTCGCCATGGCGGCGTTCTGGCATCGGTACGCCCGGA
TGACCTCTTGGCCGGAGTGATTCGCGAACTGGTACAGCGAAATGGCTTTGCGGCTTCTCTGTATGAAGATGTTGTCATTG
GTAATACTAATCAGGCGGGGGAAGATGCCCGTAATGTGGCTCGTCATGCCGGGTTATTAGCTGGGTTGCCGCAGTCCGTG
GCGGGGATCACCGTGAATAGGTTATGTGGCAGCGGCCTCGCCGCAACATTGGATGCGGCACGTATGGTGACGGTGGGCGA
GGGCGAGTTGATCATTGCCGGTGGTACGGAATCGATGAGCCGTGCCCCTTATGTAATGGCCAAAGCAGAAAAAGCGTTTA
GCCGGGAGATGACAGTTTTCGATAGCACTATCGGCGCCCGCTTCCCTAACCCGAAAGTGCTCAAGCAGTTTGGTGCCGAT
ACTATGCCGCAGACGGCACAGAATGTAGCGCACGACCTGGGTATTAGCCGTGAAGATGCGGATGCTTTTGCTATACAGTC
TCAACAACGCTATGAGGTCGCCCGTGAGCAAGGGTTTTATCAGCAGGAAGTGTTACCGGTTACGGTGCCCCAGGGGGGCA
AGGCGACACCACTGCGTGTGGCTGACGACGAACATCCCCGCCCCGGAACCTGCGTTGATAAGTTGCGACAACTGCCTGCT
ATTGTCGATGGGGGCATTATTACTGCGGGTAATGCGTCTGGGATAAATGACGGGGCGGCGGCAGTGATTGTGGGTTCGCG
TAGGGCTGGTGTGGCGGCGGGTATTGCTCCCCGTGCTCGAGTTCTGTCTGGTGCGGTTGCCGGAGTGGCGCCTCGCGTTA
TGGGCCTAGGCCCGGTGGATGCGTGCAACAAGGCATTGGCCCGGGCGGGCCTGGCGTTAGCTCAGATGGATGTGATTGAA
ATTAATGAGGCGTTTGCCGCCCAAGTGCTGGGCTGCTGTAAGCAGTTGGGCTTGGCTGGGGATGATGAGCGCCTGAACCC
AAATGGTGGCGCTATTGCTGTCGGCCATCCGTTAGGTGCGTCCGGTGCGCGTTTGGTCTTGACTGCCATGCGTCAGTTGG
AGCGAACCCGCGGTCGTTATGCACTTCTTAGTTTGTGCATTGGCGTAGGTCAGGGCGTTGCCATGGTGCTGGAGAGGGTA
GATTCATGA

Upstream 100 bases:

>100_bases
GCTTTGTGTGATTGAACAGAATGAAATGGGTGAGAGAGGCCTTTCTACCTACATATCGAGTCTCTTTGCCGCTTGAGCGT
GTAACTCCTGGGAGGATGTA

Downstream 100 bases:

>100_bases
GTGTTTTTGTATGGAATGATGCGCTATTGCTGGAAACTCAGCTCAGCGACGATGAGCGGATGATCCGTGATGCAGCGCAG
GATTACTGCCAAAGTAAACT

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MLDAYIYDGLRTPFGRHGGVLASVRPDDLLAGVIRELVQRNGFAASLYEDVVIGNTNQAGEDARNVARHAGLLAGLPQSV
AGITVNRLCGSGLAATLDAARMVTVGEGELIIAGGTESMSRAPYVMAKAEKAFSREMTVFDSTIGARFPNPKVLKQFGAD
TMPQTAQNVAHDLGISREDADAFAIQSQQRYEVAREQGFYQQEVLPVTVPQGGKATPLRVADDEHPRPGTCVDKLRQLPA
IVDGGIITAGNASGINDGAAAVIVGSRRAGVAAGIAPRARVLSGAVAGVAPRVMGLGPVDACNKALARAGLALAQMDVIE
INEAFAAQVLGCCKQLGLAGDDERLNPNGGAIAVGHPLGASGARLVLTAMRQLERTRGRYALLSLCIGVGQGVAMVLERV
DS

Sequences:

>Translated_402_residues
MLDAYIYDGLRTPFGRHGGVLASVRPDDLLAGVIRELVQRNGFAASLYEDVVIGNTNQAGEDARNVARHAGLLAGLPQSV
AGITVNRLCGSGLAATLDAARMVTVGEGELIIAGGTESMSRAPYVMAKAEKAFSREMTVFDSTIGARFPNPKVLKQFGAD
TMPQTAQNVAHDLGISREDADAFAIQSQQRYEVAREQGFYQQEVLPVTVPQGGKATPLRVADDEHPRPGTCVDKLRQLPA
IVDGGIITAGNASGINDGAAAVIVGSRRAGVAAGIAPRARVLSGAVAGVAPRVMGLGPVDACNKALARAGLALAQMDVIE
INEAFAAQVLGCCKQLGLAGDDERLNPNGGAIAVGHPLGASGARLVLTAMRQLERTRGRYALLSLCIGVGQGVAMVLERV
DS
>Mature_402_residues
MLDAYIYDGLRTPFGRHGGVLASVRPDDLLAGVIRELVQRNGFAASLYEDVVIGNTNQAGEDARNVARHAGLLAGLPQSV
AGITVNRLCGSGLAATLDAARMVTVGEGELIIAGGTESMSRAPYVMAKAEKAFSREMTVFDSTIGARFPNPKVLKQFGAD
TMPQTAQNVAHDLGISREDADAFAIQSQQRYEVAREQGFYQQEVLPVTVPQGGKATPLRVADDEHPRPGTCVDKLRQLPA
IVDGGIITAGNASGINDGAAAVIVGSRRAGVAAGIAPRARVLSGAVAGVAPRVMGLGPVDACNKALARAGLALAQMDVIE
INEAFAAQVLGCCKQLGLAGDDERLNPNGGAIAVGHPLGASGARLVLTAMRQLERTRGRYALLSLCIGVGQGVAMVLERV
DS

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=400, Percent_Identity=38.5, Blast_Score=278, Evalue=6e-75,
Organism=Homo sapiens, GI148539872, Length=397, Percent_Identity=38.2871536523929, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI4501853, Length=409, Percent_Identity=37.1638141809291, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI4557237, Length=404, Percent_Identity=32.6732673267327, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI4504327, Length=423, Percent_Identity=28.8416075650118, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI194353979, Length=399, Percent_Identity=29.0726817042606, Blast_Score=131, Evalue=1e-30,
Organism=Escherichia coli, GI1787663, Length=402, Percent_Identity=58.2089552238806, Blast_Score=450, Evalue=1e-128,
Organism=Escherichia coli, GI1788554, Length=404, Percent_Identity=42.3267326732673, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI48994986, Length=407, Percent_Identity=41.2776412776413, Blast_Score=265, Evalue=5e-72,
Organism=Escherichia coli, GI87082165, Length=409, Percent_Identity=39.8533007334963, Blast_Score=261, Evalue=4e-71,
Organism=Escherichia coli, GI1788683, Length=423, Percent_Identity=31.4420803782506, Blast_Score=151, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI133906874, Length=401, Percent_Identity=36.1596009975062, Blast_Score=259, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI25147385, Length=398, Percent_Identity=33.1658291457286, Blast_Score=187, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI17535921, Length=399, Percent_Identity=32.8320802005013, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17551802, Length=431, Percent_Identity=30.3944315545244, Blast_Score=161, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17535917, Length=404, Percent_Identity=28.960396039604, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6322031, Length=404, Percent_Identity=34.6534653465347, Blast_Score=202, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6325229, Length=406, Percent_Identity=32.512315270936, Blast_Score=181, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=39.4014962593516, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI17648125, Length=403, Percent_Identity=38.2133995037221, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24640423, Length=396, Percent_Identity=34.0909090909091, Blast_Score=198, Evalue=6e-51,
Organism=Drosophila melanogaster, GI17137578, Length=427, Percent_Identity=30.2107728337237, Blast_Score=162, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41737; Mature: 41737

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDAYIYDGLRTPFGRHGGVLASVRPDDLLAGVIRELVQRNGFAASLYEDVVIGNTNQAG
CCCHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHEECCCCCCC
EDARNVARHAGLLAGLPQSVAGITVNRLCGSGLAATLDAARMVTVGEGELIIAGGTESMS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHEEEEECCCCEEEECCCCCCC
RAPYVMAKAEKAFSREMTVFDSTIGARFPNPKVLKQFGADTMPQTAQNVAHDLGISREDA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCC
DAFAIQSQQRYEVAREQGFYQQEVLPVTVPQGGKATPLRVADDEHPRPGTCVDKLRQLPA
HHHHHCCHHHHHHHHHCCCHHHCCEEEECCCCCCCCCEEECCCCCCCCCHHHHHHHHCCH
IVDGGIITAGNASGINDGAAAVIVGSRRAGVAAGIAPRARVLSGAVAGVAPRVMGLGPVD
HHCCCEEECCCCCCCCCCCEEEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCHH
ACNKALARAGLALAQMDVIEINEAFAAQVLGCCKQLGLAGDDERLNPNGGAIAVGHPLGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCC
SGARLVLTAMRQLERTRGRYALLSLCIGVGQGVAMVLERVDS
CCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MLDAYIYDGLRTPFGRHGGVLASVRPDDLLAGVIRELVQRNGFAASLYEDVVIGNTNQAG
CCCHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHEECCCCCCC
EDARNVARHAGLLAGLPQSVAGITVNRLCGSGLAATLDAARMVTVGEGELIIAGGTESMS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHEEEEECCCCEEEECCCCCCC
RAPYVMAKAEKAFSREMTVFDSTIGARFPNPKVLKQFGADTMPQTAQNVAHDLGISREDA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCC
DAFAIQSQQRYEVAREQGFYQQEVLPVTVPQGGKATPLRVADDEHPRPGTCVDKLRQLPA
HHHHHCCHHHHHHHHHCCCHHHCCEEEECCCCCCCCCEEECCCCCCCCCHHHHHHHHCCH
IVDGGIITAGNASGINDGAAAVIVGSRRAGVAAGIAPRARVLSGAVAGVAPRVMGLGPVD
HHCCCEEECCCCCCCCCCCEEEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCHH
ACNKALARAGLALAQMDVIEINEAFAAQVLGCCKQLGLAGDDERLNPNGGAIAVGHPLGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCC
SGARLVLTAMRQLERTRGRYALLSLCIGVGQGVAMVLERVDS
CCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 10766858 [H]