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The map label for this gene is nirB1 [H]

Identifier: 110833716

GI number: 110833716

Start: 952436

End: 954415

Strand: Direct

Name: nirB1 [H]

Synonym: ABO_0855

Alternate gene names: 110833716

Gene position: 952436-954415 (Clockwise)

Preceding gene: 110833715

Following gene: 110833717

Centisome position: 30.53

GC content: 57.78

Gene sequence:

>1980_bases
GTGGAAGCAAAACGTTATCAGCCCCAACCCCGGGTACCTGATGCCCAGGCCGAACAGCCCGTCATCATTGTTGGCAATGG
CCCGGCGGGAATACAGTGCCTGGTGGAGTTGCGCCGCCGTGGTGTGACCACACCAGTGAAGCTGTTCGGTGCAGAGCATT
GGGCCCCGTATGATCGCGTCAAGCTTTCCACCTTTCTTTTCGGATCCACCGACTTTGATGATCTCACCAACTTGCCCCCT
GAACTGGATGACTCGGTAGAGCATCTGGTTGGGCGGCCTGTTACCGGGATTAATCCCGGCCGTCGCTGGGTAGAGGACAG
ACTCGGTCAGCGTCACCACTACAGTAGCCTGGTTCTGGCGCTGGGCAGTGACGCCCATATTCCTTCCATACCGGGCACAA
CCTTGTCTGGTGTGTTGCGTTTTCGCGATATGGCCGACGCCCAAGCGTTAATCGCCCGTCGCTTGCGAAGTACCCATACC
GTTGTCATTGGCGGCGGTCTTCTCGGTATTGAAGCTGCCCACGGATTGTGTCGTTTCGGCACCAACGTCACCTTGATTCA
GCATGCCGATCGGTTGATGAATCGCCAGCTTGATAGTGAAGCGGCCAGCATGGTGGCCAATAACCTGCGCGAAGCCGGTG
TGGATATACGGCTCAATACCAGTGTCCGGCGTATTAAGGGAACGGAACGTGTGGAGTCAGTGGTGCTTGCGGATGGCAGT
GAGCTGCCTTGCGACACGGTGGTCTTCGCAACGGGAATTGCCCCGCGGACAGCACTGGCCCGCAAGGCGGGGCTCAAGGT
TGCTAATGGTATTCGTGTCACTGCGAGCATGCAGACCTCAGATCCGCATATTTATGCGGTGGGCGAGTGCGCCCAGTTTG
AAGATCGGGTATGCGGCCTAGTGATGCCGGCACTGCAACAGGCCCGGGTCGCTGCGGGATGCCTTGCCGGCAGTGATGAC
AGTTATACACCGGTGGCTGATAGCACTTGGCTAAAAGTTTTGTCGTTGGAGGTATTCAGTGCCGGGCGCTTTACTGACGA
AGAGCGGGGCATGGTGCATCGCAGTCTTGTTTACCACGATAGCAATGCTGGCATTTATCGTGCCTTGATGTTGCGCGCCG
GGCGGTTGGTGGGCGTGGTTTCCGTGGGCTCTTGGGCGGAACGTCAGAAAGTGCTCAATCGCATCGCCGATGGTCGTCGC
ATACAGCCTTGGGAAGTATTGCGTTTCCGCTTCACTGGTGAGCTGAATGAAGGGGGCGCCGGGCAAGTGAGCGAATGGCC
GGCCGCCACCGTGATCTGTCAGTGCCGGGGGCTGTCCCGTCGCCAACTACAGGATTTCATGGCCGACGGCTGTGACGTGG
CCACCCTGAAATCTGTGAGCGGCGCCGGCACGGTATGTGGTGGCTGTGAGCCGCTGCTGGATAGTTTGTGCAGTGGTGGT
GCAGCTGCCGCCCGTAAGGTGCCTGGGGCCACCGCGCTAGCCATTATCTCAGCCGTTGCCGCGTTTGTTATGGCATTGGC
CTTCTGGTTGCCGTCACCGGCTCCGGCCAGCTCGGTACAGAATATTTCTCTGTTTGAACAATTTTCCCGCAACGGCTTGT
GGCGACAGTGGAGCGGCTATGCCGTGTTGGCCGTTTCTGTGCTGCTGTTGCTGATGTCCTTGAAAAAACGTTTTCCGAAA
GTGCCCATGGGGAGTTTTACGGGGTGGCGCATTGCCCATGCAGTACTGGCTTTGCTTGCTTTGTTAGCACTGTATTGGCA
CACCGGTTTTTCATTGGGGGAAAACCTAAACCGCTGGCTGATGTTGAGTTTTATCGGTGCCGCCATGGTTGGCATTACTG
CGGCGCTGCTAACCCGTCTGCAGGTTGCCATGAACGGAGTGGGGTGGCTGCGACAAGGCAGCACTTGGCTGCACATTCTG
GTGTGCTGGCCGCTGCCGACGCTACTGATCTTTCACATCGTTTCGGCGTACTACTTCTAA

Upstream 100 bases:

>100_bases
GATGACTTCAAGTCTGATACCCGCAAGTACTGGGCCTGGGCAGAACTGAAATTCTGATCCCCGACCTTCCCCGTTAGATC
CTCAGAAAAGGAGGTGTCAG

Downstream 100 bases:

>100_bases
AGGGCGGGTGCGGTGATGGGCAGTAAGAAACATCCTTCGGCGTTGTGGGCTATCTGGCTGTTTGGTTCGGTCGGCTTGGC
CATTTGGTTGAGTCTTCAAT

Product: nitrite reductase [NAD(P)H]

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 659; Mature: 659

Protein sequence:

>659_residues
MEAKRYQPQPRVPDAQAEQPVIIVGNGPAGIQCLVELRRRGVTTPVKLFGAEHWAPYDRVKLSTFLFGSTDFDDLTNLPP
ELDDSVEHLVGRPVTGINPGRRWVEDRLGQRHHYSSLVLALGSDAHIPSIPGTTLSGVLRFRDMADAQALIARRLRSTHT
VVIGGGLLGIEAAHGLCRFGTNVTLIQHADRLMNRQLDSEAASMVANNLREAGVDIRLNTSVRRIKGTERVESVVLADGS
ELPCDTVVFATGIAPRTALARKAGLKVANGIRVTASMQTSDPHIYAVGECAQFEDRVCGLVMPALQQARVAAGCLAGSDD
SYTPVADSTWLKVLSLEVFSAGRFTDEERGMVHRSLVYHDSNAGIYRALMLRAGRLVGVVSVGSWAERQKVLNRIADGRR
IQPWEVLRFRFTGELNEGGAGQVSEWPAATVICQCRGLSRRQLQDFMADGCDVATLKSVSGAGTVCGGCEPLLDSLCSGG
AAAARKVPGATALAIISAVAAFVMALAFWLPSPAPASSVQNISLFEQFSRNGLWRQWSGYAVLAVSVLLLLMSLKKRFPK
VPMGSFTGWRIAHAVLALLALLALYWHTGFSLGENLNRWLMLSFIGAAMVGITAALLTRLQVAMNGVGWLRQGSTWLHIL
VCWPLPTLLIFHIVSAYYF

Sequences:

>Translated_659_residues
MEAKRYQPQPRVPDAQAEQPVIIVGNGPAGIQCLVELRRRGVTTPVKLFGAEHWAPYDRVKLSTFLFGSTDFDDLTNLPP
ELDDSVEHLVGRPVTGINPGRRWVEDRLGQRHHYSSLVLALGSDAHIPSIPGTTLSGVLRFRDMADAQALIARRLRSTHT
VVIGGGLLGIEAAHGLCRFGTNVTLIQHADRLMNRQLDSEAASMVANNLREAGVDIRLNTSVRRIKGTERVESVVLADGS
ELPCDTVVFATGIAPRTALARKAGLKVANGIRVTASMQTSDPHIYAVGECAQFEDRVCGLVMPALQQARVAAGCLAGSDD
SYTPVADSTWLKVLSLEVFSAGRFTDEERGMVHRSLVYHDSNAGIYRALMLRAGRLVGVVSVGSWAERQKVLNRIADGRR
IQPWEVLRFRFTGELNEGGAGQVSEWPAATVICQCRGLSRRQLQDFMADGCDVATLKSVSGAGTVCGGCEPLLDSLCSGG
AAAARKVPGATALAIISAVAAFVMALAFWLPSPAPASSVQNISLFEQFSRNGLWRQWSGYAVLAVSVLLLLMSLKKRFPK
VPMGSFTGWRIAHAVLALLALLALYWHTGFSLGENLNRWLMLSFIGAAMVGITAALLTRLQVAMNGVGWLRQGSTWLHIL
VCWPLPTLLIFHIVSAYYF
>Mature_659_residues
MEAKRYQPQPRVPDAQAEQPVIIVGNGPAGIQCLVELRRRGVTTPVKLFGAEHWAPYDRVKLSTFLFGSTDFDDLTNLPP
ELDDSVEHLVGRPVTGINPGRRWVEDRLGQRHHYSSLVLALGSDAHIPSIPGTTLSGVLRFRDMADAQALIARRLRSTHT
VVIGGGLLGIEAAHGLCRFGTNVTLIQHADRLMNRQLDSEAASMVANNLREAGVDIRLNTSVRRIKGTERVESVVLADGS
ELPCDTVVFATGIAPRTALARKAGLKVANGIRVTASMQTSDPHIYAVGECAQFEDRVCGLVMPALQQARVAAGCLAGSDD
SYTPVADSTWLKVLSLEVFSAGRFTDEERGMVHRSLVYHDSNAGIYRALMLRAGRLVGVVSVGSWAERQKVLNRIADGRR
IQPWEVLRFRFTGELNEGGAGQVSEWPAATVICQCRGLSRRQLQDFMADGCDVATLKSVSGAGTVCGGCEPLLDSLCSGG
AAAARKVPGATALAIISAVAAFVMALAFWLPSPAPASSVQNISLFEQFSRNGLWRQWSGYAVLAVSVLLLLMSLKKRFPK
VPMGSFTGWRIAHAVLALLALLALYWHTGFSLGENLNRWLMLSFIGAAMVGITAALLTRLQVAMNGVGWLRQGSTWLHIL
VCWPLPTLLIFHIVSAYYF

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=278, Percent_Identity=28.7769784172662, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI226437568, Length=278, Percent_Identity=28.7769784172662, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI65787454, Length=278, Percent_Identity=28.7769784172662, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1789765, Length=466, Percent_Identity=33.0472103004292, Blast_Score=173, Evalue=4e-44,
Organism=Escherichia coli, GI1789065, Length=372, Percent_Identity=30.3763440860215, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788892, Length=277, Percent_Identity=28.8808664259928, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87082354, Length=174, Percent_Identity=28.735632183908, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=277, Percent_Identity=26.7148014440433, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI32564386, Length=337, Percent_Identity=25.5192878338279, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24585130, Length=292, Percent_Identity=27.3972602739726, Blast_Score=112, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24639257, Length=320, Percent_Identity=28.75, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281359715, Length=320, Percent_Identity=28.75, Blast_Score=110, Evalue=4e-24,
Organism=Drosophila melanogaster, GI281359713, Length=320, Percent_Identity=28.75, Blast_Score=110, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24639250, Length=320, Percent_Identity=28.75, Blast_Score=110, Evalue=4e-24,
Organism=Drosophila melanogaster, GI18543267, Length=320, Percent_Identity=28.75, Blast_Score=110, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24639252, Length=320, Percent_Identity=28.75, Blast_Score=110, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 71437; Mature: 71437

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAKRYQPQPRVPDAQAEQPVIIVGNGPAGIQCLVELRRRGVTTPVKLFGAEHWAPYDRV
CCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHE
KLSTFLFGSTDFDDLTNLPPELDDSVEHLVGRPVTGINPGRRWVEDRLGQRHHYSSLVLA
EEEEEEECCCCHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHEE
LGSDAHIPSIPGTTLSGVLRFRDMADAQALIARRLRSTHTVVIGGGLLGIEAAHGLCRFG
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHC
TNVTLIQHADRLMNRQLDSEAASMVANNLREAGVDIRLNTSVRRIKGTERVESVVLADGS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHCCHHHHHHHHEECCC
ELPCDTVVFATGIAPRTALARKAGLKVANGIRVTASMQTSDPHIYAVGECAQFEDRVCGL
CCCCCCEEEECCCCHHHHHHHHHCCEEECCEEEEEEEECCCCCEEEECCHHHHHHHHHHH
VMPALQQARVAAGCLAGSDDSYTPVADSTWLKVLSLEVFSAGRFTDEERGMVHRSLVYHD
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHCHHHHHHEEEC
SNAGIYRALMLRAGRLVGVVSVGSWAERQKVLNRIADGRRIQPWEVLRFRFTGELNEGGA
CCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHEEEEEECCCCCCCC
GQVSEWPAATVICQCRGLSRRQLQDFMADGCDVATLKSVSGAGTVCGGCEPLLDSLCSGG
CCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCC
AAAARKVPGATALAIISAVAAFVMALAFWLPSPAPASSVQNISLFEQFSRNGLWRQWSGY
CHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCH
AVLAVSVLLLLMSLKKRFPKVPMGSFTGWRIAHAVLALLALLALYWHTGFSLGENLNRWL
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHH
MLSFIGAAMVGITAALLTRLQVAMNGVGWLRQGSTWLHILVCWPLPTLLIFHIVSAYYF
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEAKRYQPQPRVPDAQAEQPVIIVGNGPAGIQCLVELRRRGVTTPVKLFGAEHWAPYDRV
CCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHE
KLSTFLFGSTDFDDLTNLPPELDDSVEHLVGRPVTGINPGRRWVEDRLGQRHHYSSLVLA
EEEEEEECCCCHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHEE
LGSDAHIPSIPGTTLSGVLRFRDMADAQALIARRLRSTHTVVIGGGLLGIEAAHGLCRFG
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHC
TNVTLIQHADRLMNRQLDSEAASMVANNLREAGVDIRLNTSVRRIKGTERVESVVLADGS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHCCHHHHHHHHEECCC
ELPCDTVVFATGIAPRTALARKAGLKVANGIRVTASMQTSDPHIYAVGECAQFEDRVCGL
CCCCCCEEEECCCCHHHHHHHHHCCEEECCEEEEEEEECCCCCEEEECCHHHHHHHHHHH
VMPALQQARVAAGCLAGSDDSYTPVADSTWLKVLSLEVFSAGRFTDEERGMVHRSLVYHD
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHCHHHHHHEEEC
SNAGIYRALMLRAGRLVGVVSVGSWAERQKVLNRIADGRRIQPWEVLRFRFTGELNEGGA
CCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHEEEEEECCCCCCCC
GQVSEWPAATVICQCRGLSRRQLQDFMADGCDVATLKSVSGAGTVCGGCEPLLDSLCSGG
CCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCC
AAAARKVPGATALAIISAVAAFVMALAFWLPSPAPASSVQNISLFEQFSRNGLWRQWSGY
CHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCH
AVLAVSVLLLLMSLKKRFPKVPMGSFTGWRIAHAVLALLALLALYWHTGFSLGENLNRWL
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHH
MLSFIGAAMVGITAALLTRLQVAMNGVGWLRQGSTWLHILVCWPLPTLLIFHIVSAYYF
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]