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The map label for this gene is colS [H]

Identifier: 110833690

GI number: 110833690

Start: 921301

End: 922560

Strand: Direct

Name: colS [H]

Synonym: ABO_0829

Alternate gene names: 110833690

Gene position: 921301-922560 (Clockwise)

Preceding gene: 110833689

Following gene: 110833694

Centisome position: 29.53

GC content: 52.3

Gene sequence:

>1260_bases
ATGAAAAAAACCGGCGTCCAGCATCGTATAACCCGTGTCTATCTGGTTCAGTTATTGCTGATCAGTCTGGCCACCGTGCT
GGGTGTATGGGCGACGGCCAATATCATTGAGCATGTGTTGGTCAAACAGGCCCTAATCAAGGAGGCCGATCATTATTGGT
CTTTGTTTGAGGCAAATTCAACGCAACCACGGCCCAATACCCGCCACTTATTCGGTTTGCTGACCAGTGACGCGGTGCAT
GATGCCGTGCCGAAGGTACTCATGGATCTACCTGATGGCTATCAACGTGTGGATCTAGCCGGAGAACGGCCTATTGTTTA
TATCGAGCATCGCGATGACGCCCGGCTTTATTTAATCTTTGATGAAAAGCGCTTGTCAGTGCTGGCATTCATGTTCGGGA
TTTTACCACTGACGGGGGTGCTGTTGGTGATCTATATCACCTCGTTTCTCGGTTGGAAAAAATCCCGCGATTTACTCTCT
CCACTGGTGCAGCTTTCTGATGCGTTGCGACAGACTCCGGTAAACGACCCAAGCGTGGCTCGCCCTGATCTAAGCGAAAT
AGCGGCGGACGCCGGCAGTGAAGTGGCTGTGCTGGTTACCGCTTTGGATGGTTATGCCAATCGGTTAGTGTCGTTTGTAG
AGCGTGAGCGCCAGTTTACCCGTGATGCCAGCCATGAGTTACGTACGCCACTGGCCGTGTTTCGAGCCAACCTGGAATTG
CTGGTGATTCAGATTGGGGATCGGCCACTGATACGGCGTATGGATGACACGGTTGATGATATGGAGGCGACGTTGGAGAC
ATTGCTGATGCTGGCTCGTACCGAGCAGCATAGCCAGCAAAGTGAAGACGTGATTGTTAATGATCTGTCGGTGAATTTGA
TCGAACGCCTTAGCCCGTTGGCCGAACGCAAGCACATTCGATTACAGGTCCGGCAGACTGCACTGTTGAGTGTGAATTGC
CCGGAAGCTGCGTTGACCATTGTGCTGACAAACCTGGTTCGCAATGCCATCAACTACAGTGGATCGGGTGATGTCACCAT
CGTGGTAATGGAGCAAGCGGTGCAGGTTGTGGATTATGGTGCGGGCATGGATGCGGAAGAATTATCGCGCATCATGCAAC
CTTTTGAGCGTGGTACGAGCATCGAAAGCGGCCATGGGTTGGGGTTGGCGATTGTGCAGCGCCTGTGCGAACGCTATCGC
TGGTCGTTGATGGTAGCTAGTGAACCCGGTAAAGGCACGGAAGTCAGAGTGCAATTCTAA

Upstream 100 bases:

>100_bases
CAAGAGTGGTTTGTTGCACACTACCGTTTCAGTAAAGCTGTTGTCAGTTGAGCCGGCGATATTGCGGCCATTGATGTAAC
GTTTTGATGATGAGTTATGC

Downstream 100 bases:

>100_bases
ATGTATATTTCTTCTCTGCGGAGCGCATTCTTATATCGCCATTTCCTGGGCTCTGTTTAGGGGGCGCTGTTCAATCCGGA
CCAGTAGTTGTTTGGCGTTT

Product: ColS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MKKTGVQHRITRVYLVQLLLISLATVLGVWATANIIEHVLVKQALIKEADHYWSLFEANSTQPRPNTRHLFGLLTSDAVH
DAVPKVLMDLPDGYQRVDLAGERPIVYIEHRDDARLYLIFDEKRLSVLAFMFGILPLTGVLLVIYITSFLGWKKSRDLLS
PLVQLSDALRQTPVNDPSVARPDLSEIAADAGSEVAVLVTALDGYANRLVSFVERERQFTRDASHELRTPLAVFRANLEL
LVIQIGDRPLIRRMDDTVDDMEATLETLLMLARTEQHSQQSEDVIVNDLSVNLIERLSPLAERKHIRLQVRQTALLSVNC
PEAALTIVLTNLVRNAINYSGSGDVTIVVMEQAVQVVDYGAGMDAEELSRIMQPFERGTSIESGHGLGLAIVQRLCERYR
WSLMVASEPGKGTEVRVQF

Sequences:

>Translated_419_residues
MKKTGVQHRITRVYLVQLLLISLATVLGVWATANIIEHVLVKQALIKEADHYWSLFEANSTQPRPNTRHLFGLLTSDAVH
DAVPKVLMDLPDGYQRVDLAGERPIVYIEHRDDARLYLIFDEKRLSVLAFMFGILPLTGVLLVIYITSFLGWKKSRDLLS
PLVQLSDALRQTPVNDPSVARPDLSEIAADAGSEVAVLVTALDGYANRLVSFVERERQFTRDASHELRTPLAVFRANLEL
LVIQIGDRPLIRRMDDTVDDMEATLETLLMLARTEQHSQQSEDVIVNDLSVNLIERLSPLAERKHIRLQVRQTALLSVNC
PEAALTIVLTNLVRNAINYSGSGDVTIVVMEQAVQVVDYGAGMDAEELSRIMQPFERGTSIESGHGLGLAIVQRLCERYR
WSLMVASEPGKGTEVRVQF
>Mature_419_residues
MKKTGVQHRITRVYLVQLLLISLATVLGVWATANIIEHVLVKQALIKEADHYWSLFEANSTQPRPNTRHLFGLLTSDAVH
DAVPKVLMDLPDGYQRVDLAGERPIVYIEHRDDARLYLIFDEKRLSVLAFMFGILPLTGVLLVIYITSFLGWKKSRDLLS
PLVQLSDALRQTPVNDPSVARPDLSEIAADAGSEVAVLVTALDGYANRLVSFVERERQFTRDASHELRTPLAVFRANLEL
LVIQIGDRPLIRRMDDTVDDMEATLETLLMLARTEQHSQQSEDVIVNDLSVNLIERLSPLAERKHIRLQVRQTALLSVNC
PEAALTIVLTNLVRNAINYSGSGDVTIVVMEQAVQVVDYGAGMDAEELSRIMQPFERGTSIESGHGLGLAIVQRLCERYR
WSLMVASEPGKGTEVRVQF

Specific function: Member of the two-component regulatory system BasS/BasR Autophosphorylates and activates BasR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790551, Length=274, Percent_Identity=31.7518248175182, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789403, Length=345, Percent_Identity=27.8260869565217, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI145693157, Length=226, Percent_Identity=27.8761061946903, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790346, Length=239, Percent_Identity=28.0334728033473, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786600, Length=217, Percent_Identity=30.4147465437788, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788393, Length=281, Percent_Identity=27.4021352313167, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082128, Length=229, Percent_Identity=27.5109170305677, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1789808, Length=292, Percent_Identity=29.1095890410959, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 46922; Mature: 46922

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTGVQHRITRVYLVQLLLISLATVLGVWATANIIEHVLVKQALIKEADHYWSLFEANS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TQPRPNTRHLFGLLTSDAVHDAVPKVLMDLPDGYQRVDLAGERPIVYIEHRDDARLYLIF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCEEEEEECCCCEEEEEE
DEKRLSVLAFMFGILPLTGVLLVIYITSFLGWKKSRDLLSPLVQLSDALRQTPVNDPSVA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
RPDLSEIAADAGSEVAVLVTALDGYANRLVSFVERERQFTRDASHELRTPLAVFRANLEL
CCCHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCEE
LVIQIGDRPLIRRMDDTVDDMEATLETLLMLARTEQHSQQSEDVIVNDLSVNLIERLSPL
EEEEECCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHH
AERKHIRLQVRQTALLSVNCPEAALTIVLTNLVRNAINYSGSGDVTIVVMEQAVQVVDYG
HHHHHHEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHCC
AGMDAEELSRIMQPFERGTSIESGHGLGLAIVQRLCERYRWSLMVASEPGKGTEVRVQF
CCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCEEEEEECCCCCCCEEEEEC
>Mature Secondary Structure
MKKTGVQHRITRVYLVQLLLISLATVLGVWATANIIEHVLVKQALIKEADHYWSLFEANS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TQPRPNTRHLFGLLTSDAVHDAVPKVLMDLPDGYQRVDLAGERPIVYIEHRDDARLYLIF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCEEEEEECCCCEEEEEE
DEKRLSVLAFMFGILPLTGVLLVIYITSFLGWKKSRDLLSPLVQLSDALRQTPVNDPSVA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
RPDLSEIAADAGSEVAVLVTALDGYANRLVSFVERERQFTRDASHELRTPLAVFRANLEL
CCCHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCEE
LVIQIGDRPLIRRMDDTVDDMEATLETLLMLARTEQHSQQSEDVIVNDLSVNLIERLSPL
EEEEECCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHH
AERKHIRLQVRQTALLSVNCPEAALTIVLTNLVRNAINYSGSGDVTIVVMEQAVQVVDYG
HHHHHHEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHCC
AGMDAEELSRIMQPFERGTSIESGHGLGLAIVQRLCERYRWSLMVASEPGKGTEVRVQF
CCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCEEEEEECCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282725; 7610040; 9278503 [H]