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The map label for this gene is tolB [H]

Identifier: 110833619

GI number: 110833619

Start: 852425

End: 853747

Strand: Direct

Name: tolB [H]

Synonym: ABO_0758

Alternate gene names: 110833619

Gene position: 852425-853747 (Clockwise)

Preceding gene: 110833618

Following gene: 110833620

Centisome position: 27.32

GC content: 52.76

Gene sequence:

>1323_bases
ATGATAGGTGTGTTAAGGATGCAGCAAACCTGTAAACGAAAGATATGGATGCAAATCAGCGTCGCTTTGGTGACATCTTT
GTGGATGATCAGTGCGCAGGCTGAGTTGCTCATCAAGGTAACTGAGGGCCGTGTGGATGCGGTGCCGATCGCTGTAGTGC
CTTTTGATGGTGCAAAAAATGCAGAGGAAGATGTCTCCGCGATCGTTCAGGCGGACCTGCACCGCAGCGGTCAGTTTAAA
CCGCTGCCGCCGCAAGACATGCTCAGCCGCCCCCGTAATGAGCAAGAGATGATTTATCGGGATTTCAAAGTGCTGGGCAC
AGAGTATGTGATCACCGGCAGCATGAAGCGTCAGGAAACCGGCGGTTACGAGGTCGGTTATGCACTGTTTGATGTGGCGC
GGCAAAAGAAATTGATGTCGGAAACCTATCGCCCGCCGGCGTCCCAGCTACGTGATGTGGCCCATGCCATCAGCGATCGT
ATCTATGAGCAGCTCACTGGGATCCCTGGAGCGTTCTCTACGAAAATTCTTTATGTGACCCATAATCGCCATGATGCCAA
TCCGTATCAGCTGCAATATGCCGATGCGGATGGCCACCGAGTAACGACCATCTTGCGTAGTAACGAACCGATAATGAGCC
CTACCTGGTCGCCAGATGGCAGGAAAATTGCTTACGTTTCCTTTGAAGATCAGGGGCGGCCAGGTATTTATATTCACAAC
CTGGCCACCACTGAACGGGAGAAGGTGTCCAGCTTTAGCGGCATTAACGGTGCACCAGACTGGTCGCCAGATGGTCAGCA
CTTGGCGTTGACCTTGTCCCGTGACGGCAATCCCGAAATTTATATGTTGAATCTGTCGACCAAGGTGTTGACCCGGTTAA
CGAACAATTACGGCATTGATACCGAGCCGCGTTGGTTGCCCGATGGCGAACACTTGGTGTTCACTTCCAGCCGTTCCGGG
GGGCCACAGATTTACAAACTGAACATAAATGATAAGTCGGTTCGGCGGGTTTCGTTCCAGGGCGGCTATAATGCGCGCTC
GGATGTAACCCCAGACGGCCGCTATCTCGTTTATGTGCATCGACGAAATGGAAATTACAATGTCGGTGTGCAGGACCTCC
AGCGTGGCACATTTAATATTGTGACCCGTACCGATATGGATGAATCGCCCAGTGTTGCGCCCAATGGAAGTATGGTAATT
TACGGGACGCAACATGGGGGAGCGGGGGTTCTGGAAGCCGTATCCATTGATGGCAGAGTCAAAGTTGAGCTGCCGTCGAA
GGAAGGGGAAGTCAGAGAGCCGGCATGGTCGCCGTTCCTCTGA

Upstream 100 bases:

>100_bases
ATGCAGTCAAGCTAGCCAGCCCACTACCGGTACCTGATGGATCGCTTTTTAACGAACAGTTTCGCTCACTGGTGATCGAA
TTCGATCCTGGAGTTGCACA

Downstream 100 bases:

>100_bases
GTGGTGCTCGAGTCCAATACTAGGAAATTATAGGAGTTTTTCGCATGCGTTCATTACTAAACCCGCTGTTTGCTCTGGTT
CTGGCAGCTGCATTGGCAGC

Product: tolB protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MIGVLRMQQTCKRKIWMQISVALVTSLWMISAQAELLIKVTEGRVDAVPIAVVPFDGAKNAEEDVSAIVQADLHRSGQFK
PLPPQDMLSRPRNEQEMIYRDFKVLGTEYVITGSMKRQETGGYEVGYALFDVARQKKLMSETYRPPASQLRDVAHAISDR
IYEQLTGIPGAFSTKILYVTHNRHDANPYQLQYADADGHRVTTILRSNEPIMSPTWSPDGRKIAYVSFEDQGRPGIYIHN
LATTEREKVSSFSGINGAPDWSPDGQHLALTLSRDGNPEIYMLNLSTKVLTRLTNNYGIDTEPRWLPDGEHLVFTSSRSG
GPQIYKLNINDKSVRRVSFQGGYNARSDVTPDGRYLVYVHRRNGNYNVGVQDLQRGTFNIVTRTDMDESPSVAPNGSMVI
YGTQHGGAGVLEAVSIDGRVKVELPSKEGEVREPAWSPFL

Sequences:

>Translated_440_residues
MIGVLRMQQTCKRKIWMQISVALVTSLWMISAQAELLIKVTEGRVDAVPIAVVPFDGAKNAEEDVSAIVQADLHRSGQFK
PLPPQDMLSRPRNEQEMIYRDFKVLGTEYVITGSMKRQETGGYEVGYALFDVARQKKLMSETYRPPASQLRDVAHAISDR
IYEQLTGIPGAFSTKILYVTHNRHDANPYQLQYADADGHRVTTILRSNEPIMSPTWSPDGRKIAYVSFEDQGRPGIYIHN
LATTEREKVSSFSGINGAPDWSPDGQHLALTLSRDGNPEIYMLNLSTKVLTRLTNNYGIDTEPRWLPDGEHLVFTSSRSG
GPQIYKLNINDKSVRRVSFQGGYNARSDVTPDGRYLVYVHRRNGNYNVGVQDLQRGTFNIVTRTDMDESPSVAPNGSMVI
YGTQHGGAGVLEAVSIDGRVKVELPSKEGEVREPAWSPFL
>Mature_440_residues
MIGVLRMQQTCKRKIWMQISVALVTSLWMISAQAELLIKVTEGRVDAVPIAVVPFDGAKNAEEDVSAIVQADLHRSGQFK
PLPPQDMLSRPRNEQEMIYRDFKVLGTEYVITGSMKRQETGGYEVGYALFDVARQKKLMSETYRPPASQLRDVAHAISDR
IYEQLTGIPGAFSTKILYVTHNRHDANPYQLQYADADGHRVTTILRSNEPIMSPTWSPDGRKIAYVSFEDQGRPGIYIHN
LATTEREKVSSFSGINGAPDWSPDGQHLALTLSRDGNPEIYMLNLSTKVLTRLTNNYGIDTEPRWLPDGEHLVFTSSRSG
GPQIYKLNINDKSVRRVSFQGGYNARSDVTPDGRYLVYVHRRNGNYNVGVQDLQRGTFNIVTRTDMDESPSVAPNGSMVI
YGTQHGGAGVLEAVSIDGRVKVELPSKEGEVREPAWSPFL

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=418, Percent_Identity=40.6698564593301, Blast_Score=327, Evalue=6e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 49163; Mature: 49163

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGVLRMQQTCKRKIWMQISVALVTSLWMISAQAELLIKVTEGRVDAVPIAVVPFDGAKN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEEECCCCCC
AEEDVSAIVQADLHRSGQFKPLPPQDMLSRPRNEQEMIYRDFKVLGTEYVITGSMKRQET
CHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCCCCC
GGYEVGYALFDVARQKKLMSETYRPPASQLRDVAHAISDRIYEQLTGIPGAFSTKILYVT
CCEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
HNRHDANPYQLQYADADGHRVTTILRSNEPIMSPTWSPDGRKIAYVSFEDQGRPGIYIHN
ECCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEE
LATTEREKVSSFSGINGAPDWSPDGQHLALTLSRDGNPEIYMLNLSTKVLTRLTNNYGID
CCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCHHHHHHHHHCCCCC
TEPRWLPDGEHLVFTSSRSGGPQIYKLNINDKSVRRVSFQGGYNARSDVTPDGRYLVYVH
CCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCEEEEEE
RRNGNYNVGVQDLQRGTFNIVTRTDMDESPSVAPNGSMVIYGTQHGGAGVLEAVSIDGRV
ECCCCCCCCHHHHCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCEE
KVELPSKEGEVREPAWSPFL
EEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIGVLRMQQTCKRKIWMQISVALVTSLWMISAQAELLIKVTEGRVDAVPIAVVPFDGAKN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEEECCCCCC
AEEDVSAIVQADLHRSGQFKPLPPQDMLSRPRNEQEMIYRDFKVLGTEYVITGSMKRQET
CHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCCCCC
GGYEVGYALFDVARQKKLMSETYRPPASQLRDVAHAISDRIYEQLTGIPGAFSTKILYVT
CCEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
HNRHDANPYQLQYADADGHRVTTILRSNEPIMSPTWSPDGRKIAYVSFEDQGRPGIYIHN
ECCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEE
LATTEREKVSSFSGINGAPDWSPDGQHLALTLSRDGNPEIYMLNLSTKVLTRLTNNYGID
CCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCHHHHHHHHHCCCCC
TEPRWLPDGEHLVFTSSRSGGPQIYKLNINDKSVRRVSFQGGYNARSDVTPDGRYLVYVH
CCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCEEEEEE
RRNGNYNVGVQDLQRGTFNIVTRTDMDESPSVAPNGSMVIYGTQHGGAGVLEAVSIDGRV
ECCCCCCCCHHHHCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCEE
KVELPSKEGEVREPAWSPFL
EEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA