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The map label for this gene is nfdA [H]

Identifier: 110833566

GI number: 110833566

Start: 787293

End: 789050

Strand: Direct

Name: nfdA [H]

Synonym: ABO_0705

Alternate gene names: 110833566

Gene position: 787293-789050 (Clockwise)

Preceding gene: 110833557

Following gene: 110833569

Centisome position: 25.23

GC content: 58.42

Gene sequence:

>1758_bases
ATGCACCGGCCAGCATTGAGAGAGAGAGCGATGAAGAAAGCTGGGTTGTGGGTTGTGGTGGTGGTGATCGCCTTGCTGAT
CTTAACCGCGGTAGGGGTTCATCGTTGGTTTAATCCGCCGGCGCCGGCGGCCCAACTGTTCTTCAATGGTACGGTGCTGA
CCATGGACCCGCAGCAGCCAGTAGCAGACGCGATGCTAGTTAAGCATGGTGAAATCGTTGCGTTGGGTGAGGCCGAAGTG
TTGCGTCAGCAGGTCAGCCGCGATGTGATGGTGATCGATTTGGAGGGCGGCACGCTAATGCCCGGGTTTATTGAAGCGCA
TGGATATTTCCCCGGTGAAGGGTTATCGGCAGTGGCGGTGGATGCTAACAGTCCTCCCATTGGCGATTTACACAGCATAG
ACACTTTGCTGGAGCGCTTGAGTCTTTTGGCGCAGCGTCGCTCAGAAGGGGTGCTGCTTGCCTACGGTTTTGACCACACG
GCTATCGGTGAGCAACGTTACCCCACCCGCTCGGAGTTGGATTCAGTAAGCGAGACGCGACCGATTGTGGTGATGCATCG
CTCCGGGCATTTTGGGGTCGTTAATTCCGCCGCGCTGAGCAGTATGGGGATCAAGGAAGATGTCACAGATCCTACCGGTG
GTTTTTATGGGCGCGATGAAACCGGGCGGCTCAATGGCTTGCTAGCAGAAGCGGCGTTTTATCCAGTGCGTAGCAAGGCA
TTGGCGTTGGCAAGCATGGACCTTTTGAAGGTGTTGTCCCGGGCATCGCATATCTATCTGTCCCGTGGCATTACTTTTGC
GCAGAATGGATTGGCAGACCCGGCAGCGATTCGAGCTTTGGAACCGGCGCTACAGTTCGGGCTGATTCCGCTGCGCTTGA
GTCTCTGGCCTAACACGGAAGCGGAACAACAGCGACAGTCTGGGAAACTCGACATTCCGGAGGGCGATCGTATTCACCTG
GGGCCGGTGAAACTGGTCAGCGAGGGTTCGCTGCAAGGCTACAGTGGTTTTTTCGGCTCGCCGTATTATTCGTTGCCGGA
TGATTCCCCAGAGGACGATTTGGGGGGGGGCGCGTTATCTCAGCAGGCGCTTAATGATCAGGTGCAACAGTTTCATTGCC
AGGGCCGGCAGGTGGCCGTGCATGCCAATGGCCATGCCTCCATTGATCAGAGCTTGAATGCTATCGCCGGTGCACGATCG
GCCTGCCCGCAGGTGCGAGTGCGGCCGGTGTTGATTCATGTGCAAATGGCCACCCCGGAGCAGCTGCAAAGAATGGTGGC
GCTGGATGTGATTCCTTCTTTCTTCAATGTCGATATGTCTTACTGGGGCGATCGCCACCAGGATATTTTCCCGGGGCAGG
AGCCGGCACGGCAGGTAAGTCCGCTGGCACAAGCGGCACAAGCCGGCTTGCGCTTTACCCTGCATGCAGATTCCCCAATG
GTACCGTTTGAGCCGTTGCAGCTGGTGTGGAACGCGGTTGAACGCAAGACCGCCTCTGGCGCTTTGTTGGGCCCGGCACA
GGCAGTAGGCGTGGAGCGGGCGTTGCAAGCGGTGACAGTGGATGCGGCTAAACAGCTTGGGGTGGCCGATCAGGTGGGGC
AGTTGCGCCCAGGAATGCGTGCGGACTTGGTTTGGATGGACCGGGATCCACGGGAAGCCATTGGTGACTGGAGGGAGATT
CAGGTGCTAGGCACGTGGGTGGGAGGGGTGCGCCGCTACCCGAGCGGGCAGGCGGAGCGGCCAAGGTCAGCCATGTAG

Upstream 100 bases:

>100_bases
AAAGCAGAAGGGGTTTTGGCCTTAGAGACAGCAAGCTTGTTCTCCGCGGTGGAGAAGGGCAGAGTCTGCCCTTAATGAGT
GAAGGCTTGTGGGTGGCTAA

Downstream 100 bases:

>100_bases
CCCCCGTTTGCGCAGAGCGCGGGGTCGGCGCTCTGCGCAAAGCGCGTTGAATTAGCGCTAGTGCCAAGTTCCTTCTAAAT
TTTCACATTGATCGAGGAAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MHRPALRERAMKKAGLWVVVVVIALLILTAVGVHRWFNPPAPAAQLFFNGTVLTMDPQQPVADAMLVKHGEIVALGEAEV
LRQQVSRDVMVIDLEGGTLMPGFIEAHGYFPGEGLSAVAVDANSPPIGDLHSIDTLLERLSLLAQRRSEGVLLAYGFDHT
AIGEQRYPTRSELDSVSETRPIVVMHRSGHFGVVNSAALSSMGIKEDVTDPTGGFYGRDETGRLNGLLAEAAFYPVRSKA
LALASMDLLKVLSRASHIYLSRGITFAQNGLADPAAIRALEPALQFGLIPLRLSLWPNTEAEQQRQSGKLDIPEGDRIHL
GPVKLVSEGSLQGYSGFFGSPYYSLPDDSPEDDLGGGALSQQALNDQVQQFHCQGRQVAVHANGHASIDQSLNAIAGARS
ACPQVRVRPVLIHVQMATPEQLQRMVALDVIPSFFNVDMSYWGDRHQDIFPGQEPARQVSPLAQAAQAGLRFTLHADSPM
VPFEPLQLVWNAVERKTASGALLGPAQAVGVERALQAVTVDAAKQLGVADQVGQLRPGMRADLVWMDRDPREAIGDWREI
QVLGTWVGGVRRYPSGQAERPRSAM

Sequences:

>Translated_585_residues
MHRPALRERAMKKAGLWVVVVVIALLILTAVGVHRWFNPPAPAAQLFFNGTVLTMDPQQPVADAMLVKHGEIVALGEAEV
LRQQVSRDVMVIDLEGGTLMPGFIEAHGYFPGEGLSAVAVDANSPPIGDLHSIDTLLERLSLLAQRRSEGVLLAYGFDHT
AIGEQRYPTRSELDSVSETRPIVVMHRSGHFGVVNSAALSSMGIKEDVTDPTGGFYGRDETGRLNGLLAEAAFYPVRSKA
LALASMDLLKVLSRASHIYLSRGITFAQNGLADPAAIRALEPALQFGLIPLRLSLWPNTEAEQQRQSGKLDIPEGDRIHL
GPVKLVSEGSLQGYSGFFGSPYYSLPDDSPEDDLGGGALSQQALNDQVQQFHCQGRQVAVHANGHASIDQSLNAIAGARS
ACPQVRVRPVLIHVQMATPEQLQRMVALDVIPSFFNVDMSYWGDRHQDIFPGQEPARQVSPLAQAAQAGLRFTLHADSPM
VPFEPLQLVWNAVERKTASGALLGPAQAVGVERALQAVTVDAAKQLGVADQVGQLRPGMRADLVWMDRDPREAIGDWREI
QVLGTWVGGVRRYPSGQAERPRSAM
>Mature_585_residues
MHRPALRERAMKKAGLWVVVVVIALLILTAVGVHRWFNPPAPAAQLFFNGTVLTMDPQQPVADAMLVKHGEIVALGEAEV
LRQQVSRDVMVIDLEGGTLMPGFIEAHGYFPGEGLSAVAVDANSPPIGDLHSIDTLLERLSLLAQRRSEGVLLAYGFDHT
AIGEQRYPTRSELDSVSETRPIVVMHRSGHFGVVNSAALSSMGIKEDVTDPTGGFYGRDETGRLNGLLAEAAFYPVRSKA
LALASMDLLKVLSRASHIYLSRGITFAQNGLADPAAIRALEPALQFGLIPLRLSLWPNTEAEQQRQSGKLDIPEGDRIHL
GPVKLVSEGSLQGYSGFFGSPYYSLPDDSPEDDLGGGALSQQALNDQVQQFHCQGRQVAVHANGHASIDQSLNAIAGARS
ACPQVRVRPVLIHVQMATPEQLQRMVALDVIPSFFNVDMSYWGDRHQDIFPGQEPARQVSPLAQAAQAGLRFTLHADSPM
VPFEPLQLVWNAVERKTASGALLGPAQAVGVERALQAVTVDAAKQLGVADQVGQLRPGMRADLVWMDRDPREAIGDWREI
QVLGTWVGGVRRYPSGQAERPRSAM

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 63399; Mature: 63399

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRPALRERAMKKAGLWVVVVVIALLILTAVGVHRWFNPPAPAAQLFFNGTVLTMDPQQP
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEECCEEEEECCCCC
VADAMLVKHGEIVALGEAEVLRQQVSRDVMVIDLEGGTLMPGFIEAHGYFPGEGLSAVAV
HHHHHHHCCCCEEEECCHHHHHHHHCCCEEEEEECCCEECCCHHEECCCCCCCCCEEEEE
DANSPPIGDLHSIDTLLERLSLLAQRRSEGVLLAYGFDHTAIGEQRYPTRSELDSVSETR
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCC
PIVVMHRSGHFGVVNSAALSSMGIKEDVTDPTGGFYGRDETGRLNGLLAEAAFYPVRSKA
CEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LALASMDLLKVLSRASHIYLSRGITFAQNGLADPAAIRALEPALQFGLIPLRLSLWPNTE
HHHHHHHHHHHHHHHHHEEEECCCCHHHCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCH
AEQQRQSGKLDIPEGDRIHLGPVKLVSEGSLQGYSGFFGSPYYSLPDDSPEDDLGGGALS
HHHHHHCCCCCCCCCCEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
QQALNDQVQQFHCQGRQVAVHANGHASIDQSLNAIAGARSACPQVRVRPVLIHVQMATPE
HHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCHH
QLQRMVALDVIPSFFNVDMSYWGDRHQDIFPGQEPARQVSPLAQAAQAGLRFTLHADSPM
HHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCC
VPFEPLQLVWNAVERKTASGALLGPAQAVGVERALQAVTVDAAKQLGVADQVGQLRPGMR
CCCHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCC
ADLVWMDRDPREAIGDWREIQVLGTWVGGVRRYPSGQAERPRSAM
EEEEEECCCHHHHHCCCEEEEEEEHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MHRPALRERAMKKAGLWVVVVVIALLILTAVGVHRWFNPPAPAAQLFFNGTVLTMDPQQP
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEECCEEEEECCCCC
VADAMLVKHGEIVALGEAEVLRQQVSRDVMVIDLEGGTLMPGFIEAHGYFPGEGLSAVAV
HHHHHHHCCCCEEEECCHHHHHHHHCCCEEEEEECCCEECCCHHEECCCCCCCCCEEEEE
DANSPPIGDLHSIDTLLERLSLLAQRRSEGVLLAYGFDHTAIGEQRYPTRSELDSVSETR
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCC
PIVVMHRSGHFGVVNSAALSSMGIKEDVTDPTGGFYGRDETGRLNGLLAEAAFYPVRSKA
CEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LALASMDLLKVLSRASHIYLSRGITFAQNGLADPAAIRALEPALQFGLIPLRLSLWPNTE
HHHHHHHHHHHHHHHHHEEEECCCCHHHCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCH
AEQQRQSGKLDIPEGDRIHLGPVKLVSEGSLQGYSGFFGSPYYSLPDDSPEDDLGGGALS
HHHHHHCCCCCCCCCCEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
QQALNDQVQQFHCQGRQVAVHANGHASIDQSLNAIAGARSACPQVRVRPVLIHVQMATPE
HHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCHH
QLQRMVALDVIPSFFNVDMSYWGDRHQDIFPGQEPARQVSPLAQAAQAGLRFTLHADSPM
HHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCC
VPFEPLQLVWNAVERKTASGALLGPAQAVGVERALQAVTVDAAKQLGVADQVGQLRPGMR
CCCHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCC
ADLVWMDRDPREAIGDWREIQVLGTWVGGVRRYPSGQAERPRSAM
EEEEEECCCHHHHHCCCEEEEEEEHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA