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The map label for this gene is livK [H]

Identifier: 110680749

GI number: 110680749

Start: 3453181

End: 3454350

Strand: Direct

Name: livK [H]

Synonym: RD1_3591

Alternate gene names: 110680749

Gene position: 3453181-3454350 (Clockwise)

Preceding gene: 110680748

Following gene: 110680754

Centisome position: 83.55

GC content: 59.15

Gene sequence:

>1170_bases
ATGAAACACCTTTTGACGGCCACGACACTGGCACTCAGCCTTGGCACAACGGCGATCGCAGATGAAATCACGATCGGCTT
TGCAATCGCAAAGTCCGGCTGGGTCGAAGCCTACGACGCGCCCGCAGTGGCAGCCGCGCGCATCCGCATTGACGAAATCA
ACGCAGCAGGCGGTCTGCTGGGCAAGCAGATCAGATGGATCGAAGCCGACACGAAAAGCGATCAGGCGCAATCTGCAAGC
GCAGGCCTACAGATGGTGGACGAAGGTGCAGACATGTTGATCGTGTCCTGTGACTACGACTTCGGCGCGCCTGCGGCTTT
GGCGGCTGAAGGAGCTGGGCTCAACTCCTTCTTCCTGTGTGCCGAAGACATCAAGGCGGGCATTCAGGGCGTTGGCCCCA
ACAGTTTCTCGGCCTCCGTTCTCGCAGCCGTTCAGGGCGCGACGGTTGCGGAATGGTCCGTGCAGGAGCGCGATGCCAAA
ACCGGCTACGTGCTTCTGGATACGACCATTGAATATAACAAGGGCATCTGCACCGGCTTTGACTGGATGTTCCCCAAGGC
AGGGGGCGAGATTGTGGGCACCGACACTTTCCTGAACGGTGACGCCTCAATTGCGAGCCAGATCACCCGCATCAAAAGCC
TCGCTGAGGAGCCGGACGTGATCATGCTGTGTTCCTACATTCCGGGTGCGGCCTCTGCCGTGCGTCAGATCCGGGCTGCC
GGGATCAATTCGACGATCCTCAATGGTTCTGCGGTGGACGGTTCTTACTGGCTTGACGCGGCGCCTGATCTGTCGAACTT
CATCGTACCCGTTCAGGGGTCCATCTATGGCGATGACACACGCCCTGCCGTGAACGCGCTTCGCGATGCATTTGAGGCGG
CAACAGGCGCAGGCCCGTCCAGCACCTATGCCTATCCGGGTTACCTGCTGATTGATCTGTGGGCAAAGGCGGTTGAACGC
GCCGGCACCGTCGAGGCATCGGCTGTAACCGCTGAACTGGAAAAAATGGACAACGAGCCAACGGCCTTTGGTCCGCGCAG
CTTCTCGGATCAACTCCACCACCAGAATACGGCAGAGATGCAGATCGTGGAAATCACCAATGGCAAGCCGGGCGTGATTG
GCAGCTTCACGATCAGCGAGCCTGTGCCGCTGGATGTGCTGTTGAAGTAG

Upstream 100 bases:

>100_bases
CTGCGTCCCACCGGGCTGACAGGGGGGCGTGAACTCCGTCTGCGGATCGGTGGTCGCTGACGGGGAAACAACAACACCAA
CACTCAACGGGAGAAGACAT

Downstream 100 bases:

>100_bases
AGTCCGTTTTCCATGGTCAAAGCAGCAGAGTGCCCCAATCCGGGGCACTCTTTTTTTCTGTAAGGCTTGTGAGAGTACCC
CGCAGACACCCAAGGAACAC

Product: branched-chain amino acid ABC transporter, periplasmic binding protein, putative

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: L-BP; LS-BP [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MKHLLTATTLALSLGTTAIADEITIGFAIAKSGWVEAYDAPAVAAARIRIDEINAAGGLLGKQIRWIEADTKSDQAQSAS
AGLQMVDEGADMLIVSCDYDFGAPAALAAEGAGLNSFFLCAEDIKAGIQGVGPNSFSASVLAAVQGATVAEWSVQERDAK
TGYVLLDTTIEYNKGICTGFDWMFPKAGGEIVGTDTFLNGDASIASQITRIKSLAEEPDVIMLCSYIPGAASAVRQIRAA
GINSTILNGSAVDGSYWLDAAPDLSNFIVPVQGSIYGDDTRPAVNALRDAFEAATGAGPSSTYAYPGYLLIDLWAKAVER
AGTVEASAVTAELEKMDNEPTAFGPRSFSDQLHHQNTAEMQIVEITNGKPGVIGSFTISEPVPLDVLLK

Sequences:

>Translated_389_residues
MKHLLTATTLALSLGTTAIADEITIGFAIAKSGWVEAYDAPAVAAARIRIDEINAAGGLLGKQIRWIEADTKSDQAQSAS
AGLQMVDEGADMLIVSCDYDFGAPAALAAEGAGLNSFFLCAEDIKAGIQGVGPNSFSASVLAAVQGATVAEWSVQERDAK
TGYVLLDTTIEYNKGICTGFDWMFPKAGGEIVGTDTFLNGDASIASQITRIKSLAEEPDVIMLCSYIPGAASAVRQIRAA
GINSTILNGSAVDGSYWLDAAPDLSNFIVPVQGSIYGDDTRPAVNALRDAFEAATGAGPSSTYAYPGYLLIDLWAKAVER
AGTVEASAVTAELEKMDNEPTAFGPRSFSDQLHHQNTAEMQIVEITNGKPGVIGSFTISEPVPLDVLLK
>Mature_389_residues
MKHLLTATTLALSLGTTAIADEITIGFAIAKSGWVEAYDAPAVAAARIRIDEINAAGGLLGKQIRWIEADTKSDQAQSAS
AGLQMVDEGADMLIVSCDYDFGAPAALAAEGAGLNSFFLCAEDIKAGIQGVGPNSFSASVLAAVQGATVAEWSVQERDAK
TGYVLLDTTIEYNKGICTGFDWMFPKAGGEIVGTDTFLNGDASIASQITRIKSLAEEPDVIMLCSYIPGAASAVRQIRAA
GINSTILNGSAVDGSYWLDAAPDLSNFIVPVQGSIYGDDTRPAVNALRDAFEAATGAGPSSTYAYPGYLLIDLWAKAVER
AGTVEASAVTAELEKMDNEPTAFGPRSFSDQLHHQNTAEMQIVEITNGKPGVIGSFTISEPVPLDVLLK

Specific function: This protein is a component of the leucine-specific transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids in E.coli [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=350, Percent_Identity=24, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40682; Mature: 40682

Theoretical pI: Translated: 4.11; Mature: 4.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLLTATTLALSLGTTAIADEITIGFAIAKSGWVEAYDAPAVAAARIRIDEINAAGGLL
CCCHHHHHHHHHHCCCHHHHHHHEEEEEEECCCCCEECCCCCEEEEEEEEEECCCCCCCC
GKQIRWIEADTKSDQAQSASAGLQMVDEGADMLIVSCDYDFGAPAALAAEGAGLNSFFLC
CCEEEEEECCCCCCHHHHHHHCHHEECCCCCEEEEEECCCCCCCHHHHCCCCCCCEEEEE
AEDIKAGIQGVGPNSFSASVLAAVQGATVAEWSVQERDAKTGYVLLDTTIEYNKGICTGF
HHHHHHHHCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEEEEECCCCEECCC
DWMFPKAGGEIVGTDTFLNGDASIASQITRIKSLAEEPDVIMLCSYIPGAASAVRQIRAA
CCCCCCCCCEEEECCEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHC
GINSTILNGSAVDGSYWLDAAPDLSNFIVPVQGSIYGDDTRPAVNALRDAFEAATGAGPS
CCCCEEECCCCCCCCEEECCCCCCCCCEEEECCCEECCCCCHHHHHHHHHHHHHCCCCCC
STYAYPGYLLIDLWAKAVERAGTVEASAVTAELEKMDNEPTAFGPRSFSDQLHHQNTAEM
CCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCE
QIVEITNGKPGVIGSFTISEPVPLDVLLK
EEEEECCCCCCEEEEEECCCCCCCEEEEC
>Mature Secondary Structure
MKHLLTATTLALSLGTTAIADEITIGFAIAKSGWVEAYDAPAVAAARIRIDEINAAGGLL
CCCHHHHHHHHHHCCCHHHHHHHEEEEEEECCCCCEECCCCCEEEEEEEEEECCCCCCCC
GKQIRWIEADTKSDQAQSASAGLQMVDEGADMLIVSCDYDFGAPAALAAEGAGLNSFFLC
CCEEEEEECCCCCCHHHHHHHCHHEECCCCCEEEEEECCCCCCCHHHHCCCCCCCEEEEE
AEDIKAGIQGVGPNSFSASVLAAVQGATVAEWSVQERDAKTGYVLLDTTIEYNKGICTGF
HHHHHHHHCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEEEEECCCCEECCC
DWMFPKAGGEIVGTDTFLNGDASIASQITRIKSLAEEPDVIMLCSYIPGAASAVRQIRAA
CCCCCCCCCEEEECCEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHC
GINSTILNGSAVDGSYWLDAAPDLSNFIVPVQGSIYGDDTRPAVNALRDAFEAATGAGPS
CCCCEEECCCCCCCCEEECCCCCCCCCEEEECCCEECCCCCHHHHHHHHHHHHHCCCCCC
STYAYPGYLLIDLWAKAVERAGTVEASAVTAELEKMDNEPTAFGPRSFSDQLHHQNTAEM
CCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCE
QIVEITNGKPGVIGSFTISEPVPLDVLLK
EEEEECCCCCCEEEEEECCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3891753; 4077929; 2195019; 8041620; 9278503; 6990419; 9298646; 2649683 [H]